Fatal Disseminated Paracoccidioidomycosis In A Two-year-old Child.

A two year-old female child was admitted at the Pediatric Intensive Care Unit in a septic shock associated with a lymphoproliferative syndrome, with history of fever, adynamia and weight loss during the last two months. On admission, the main clinical and laboratory manifestations were: pallor, jaundice, disseminated enlarged lymph nodes, hepatosplenomegaly, crusted warts on face, anemia, eosinophilia, thrombocytopenia, increased direct and indirect bilirubin, alkaline phosphatase, and gammaglutamyl transpeptidase. A parenteral administration of fluids, dobutamine and mechanical ventilation was started, without improvement of the clinical conditions. A direct examination of exsudate collected from cervical lymph node revealed numerous oval-to-around cells with multiple budding, like a pilot wheel cell, suggesting Paracoccidioides brasiliensis. Even though treatment with intravenous sulfamethoxazole-trimethoprine was soon started, the child died 36 hours after hospital admission. Disseminated paracoccidioidomycosis was confirmed in the autopsy. This is the youngest case of paracoccidioidomycosis in children reported in the literature.4637-

Characterization of \u3ci\u3eNeofabraea actinidiae\u3c/i\u3e and \u3ci\u3eN. brasiliensis\u3c/i\u3e as causal agents of apple bull’s-eye rot in southern Brazil

The causal agents of apple bull’s-eye rot in southern Brazil have recently been described as Neofabraea actinidiae and N. brasiliensis. Isolates of both species were evaluated for response of mycelial growth index (MGI) to different temperatures, enzyme production, mycelial growth inhibition and effective concentrations (EC50 and EC100) of the fungicides triflumizole, pyrimethanil and thiophanate methyl, as well as aggressiveness on fruits of ‘Fuji’ hybrid and ‘Pink Lady’. There was significantly lower mycelium growth in N. brasiliensis compared with N. actinidiae at all temperatures tested. Neither species grew at 3 and 32°C. There were minor differences in production of enzymes in the two species, with all N. brasiliensis isolates showing no production of pectolyase at pH 7. The lowest EC50 and EC100 values were observed with thiophanate methyl. In general, ‘Fuji’ fruits were more susceptible to Neofabraea infection and had larger lesions, while N. brasiliensis isolates showed greater aggressiveness on ‘Fuji’ hybrid and ‘Pink lady’ fruits compared with N. actinidiae. Dans le sud du Brésil, les agents causaux du chancre gloésporien de la pomme ont récemment été décrits comme étant Neofabraea actinidiae et N. brasiliensis. Des isolats des deux espèces ont été évalués en fonction de la réaction de l’indice de croissance mycélienne à différentes températures, de la production enzymatique, de l’inhibition de la croissance mycélienne et des concentrations efficaces (CE50 et CE100) des fongicides triflumizole, pyriméthanil et triophanate méthyle, ainsi qu’en fonction de leur agressivité à l’égard de la pomme hybride Fuji et de la Pink Lady. La croissance mycélienne chez N. brasiliensis était considérablement plus faible que chez N. actinidiae, et ce, à toutes les températures testées. Aucune espèce ne s’est développée à 3 ou à 32°C. Chez les deux espèces, il y a eu de petites différences quant à la production enzymatique, tous les isolats de N. brasiliensis n’affichant aucune production de pectolyase à pH 7. Les plus faibles valeurs de CE50 et de CE100 observées ont été celles du triophanate méthyle. Dans l’ensemble, les Fuji étaient plus sujettes à l’infection causée par Neofabraea et affichaient des lésions plus étendues, tandis que les isolats de N. brasiliensis étaient plus agressifs à l’égard de l’hybride Fuji et de la Pink Lady que ceux de N. actinidiae

Characterization of \u3ci\u3eNeofabraea actinidiae\u3c/i\u3e and \u3ci\u3eN. brasiliensis\u3c/i\u3e as causal agents of apple bull’s-eye rot in southern Brazil

The causal agents of apple bull’s-eye rot in southern Brazil have recently been described as Neofabraea actinidiae and N. brasiliensis. Isolates of both species were evaluated for response of mycelial growth index (MGI) to different temperatures, enzyme production, mycelial growth inhibition and effective concentrations (EC50 and EC100) of the fungicides triflumizole, pyrimethanil and thiophanate methyl, as well as aggressiveness on fruits of ‘Fuji’ hybrid and ‘Pink Lady’. There was significantly lower mycelium growth in N. brasiliensis compared with N. actinidiae at all temperatures tested. Neither species grew at 3 and 32°C. There were minor differences in production of enzymes in the two species, with all N. brasiliensis isolates showing no production of pectolyase at pH 7. The lowest EC50 and EC100 values were observed with thiophanate methyl. In general, ‘Fuji’ fruits were more susceptible to Neofabraea infection and had larger lesions, while N. brasiliensis isolates showed greater aggressiveness on ‘Fuji’ hybrid and ‘Pink lady’ fruits compared with N. actinidiae. Dans le sud du Brésil, les agents causaux du chancre gloésporien de la pomme ont récemment été décrits comme étant Neofabraea actinidiae et N. brasiliensis. Des isolats des deux espèces ont été évalués en fonction de la réaction de l’indice de croissance mycélienne à différentes températures, de la production enzymatique, de l’inhibition de la croissance mycélienne et des concentrations efficaces (CE50 et CE100) des fongicides triflumizole, pyriméthanil et triophanate méthyle, ainsi qu’en fonction de leur agressivité à l’égard de la pomme hybride Fuji et de la Pink Lady. La croissance mycélienne chez N. brasiliensis était considérablement plus faible que chez N. actinidiae, et ce, à toutes les températures testées. Aucune espèce ne s’est développée à 3 ou à 32°C. Chez les deux espèces, il y a eu de petites différences quant à la production enzymatique, tous les isolats de N. brasiliensis n’affichant aucune production de pectolyase à pH 7. Les plus faibles valeurs de CE50 et de CE100 observées ont été celles du triophanate méthyle. Dans l’ensemble, les Fuji étaient plus sujettes à l’infection causée par Neofabraea et affichaient des lésions plus étendues, tandis que les isolats de N. brasiliensis étaient plus agressifs à l’égard de l’hybride Fuji et de la Pink Lady que ceux de N. actinidiae

Synergism and negative interference during co-infection of tomato and Nicotiana benthamiana with two bipartite begomoviruses

In Brazil, at least eight begomoviruses including Tomato rugose mosaic virus (ToRMV) and Tomato yellow spot virus (ToYSV) infect tomatoes. ToYSV symptoms in tomato and Nicotiana benthamiana appear earlier and are more severe compared to those of ToRMV. We investigated the role of several factors in this differential adaptation. To analyze infection kinetics, a single leaf was inoculated and subsequently detached after different periods of time. Viral DNA accumulation was quantified in plants, viral replication was analyzed in protoplasts, and tissue tropism was determined by in situ hybridization. Results indicate that ToYSV establishes a systemic infection and reaches a higher concentration earlier than ToRMV in both hosts. ToRMV negatively interferes with ToYSV during the initial stages of infection, but once systemic infection is established this interference ceases. In N. benthamiana, ToYSV invades the mesophyll, while ToRMV is phloem-restricted. During dual infection in this host, ToYSV releases ToRMV from the phloem

Knitted textile KTPs for instrumented underwater building systems

[Excerpt] The main scope of this study was to assess the potential of knitted textiles for underwater instrumented underwater building systems, using textile knitted preforms (multi-shape; multimaterial; etc…), using natural fibres (Hemp, Linen and Cotton)

A self-organizing map clustering approach to support territorial zoning

This work aims to evaluate three strategies for analyzing clusters of ordinal categorical data (thematic maps) to support the territorial zoning of the Alto Taquari basin, MS/MT. We evaluated a model-based method, another based on the segmentation of the multi-way contingency table, and the last one based on the transformation of ordinal data into intervals and subsequent analysis of clusters from a proposed method of segmentation of the Self-Organizing Map after the neural network training process. The results showed the adequacy of the methods based on the Self-Organizen Map and the segmentation of the contingency table, as these techniques generated unimodal clusters with distinguishable groups.Supported by National Council for Scientific and Technological Development –CNPq, Brazil, and by National Funds through the Portuguese funding agency, FCT -Fundação para a Ciência e a Tecnologia within project 2022.06822.PTDC. The work of Pedro Oliveira was also supported by the doctoral Grant PRT/BD/154311/2022 financed by the Portuguese Foundation for Science and Technology (FCT), and with funds from European Union, under MIT Portugal Progra

Projeto vetor: uma inovação de sucesso no ensino da engenharia / Vector project: a successful innovation in engineering teaching

 O presente trabalho visa descrever a experiência da gestão do Centro Acadêmico sobre o Projeto Vetor, aplicado à disciplina Introdução a Engenharia Civil na Universidade Federal de São João Del Rei, campus Alto Paraopeba, durante o período letivo de 2016/1 a 2016/2. O Projeto Vetor, iniciativa embasada nas necessidades do ensino de Engenharia no Brasil, desenvolve e incentiva a produção de pesquisa através de atividades acadêmicas e práticas que estimulem a busca por conhecimento, aumentando o interesse e o desempenho dos alunos ingressantes do curso