515 research outputs found
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Arthur Garfield Dove's landscape assemblages: a unique intersection of European modernism, American ideas, and nature-based abstraction
In the middle of his career, Arthur Garfield Dove created a smell yet novel body of landscape assemblages. They illustrate Dove's central interest in evoking nature--its motifs and rhythms--through imaginative associations of organic and man-made materials. These works represent Dove's synthesis of contemporary European stylistic and intellectual ideas as well as American philosophies and concerns. They also reflect the influence of Alfred Stieglitz and his circle and the artist Helen Torr, Dove's second wife. This study examines how Dove used a complex interplay of European theory and technique, American ideas and his own nature-based abstract style to create the landscape assemblages, works that are uniquely independent in the history of American art
Isolation of Unknown Genes from Human Bone Marrow by Differental Screening and Single-Pass cDNA Sequences Determination
A cDNA sequencing project was initiated to characterize gene expression in human bone marrow and develop strategies to isolate novel genes. Forty-eight random cDNAs from total human bone marrow were subjected to single-pass DNA sequence analysis to determine a limited complexity of mRNAs expressed in the bone marrow. Overall, 8 cDNAs (17%) showed no similarity to known sequences. Information from DNA sequence analysis was used to develop a differential prescreen to subtract unwanted cDNAs and to enrich for unknown cDNAs. Forty-eight cDNAs that were negative with a complex probe were subject to single-pass DNA sequence determination. Of these prescreened cDNAs, the number of unknown sequences increased to 23 (48%). Unknown cDNAs were also characterized by RNA expression analysis using 25 different human leukemic cell lines. Of 13 unknown cDNAs tested, 10 were expressed in all cell types tested and 3 revealed a hematopoietic lineage-restricted expression pattern. Interestingly, while a total of only 96 bone marrow cDNAs were sequenced, 31 of these cDNAs represent sequences from unknown genes and 12 showed significant similarities to sequences in the data bases. One cDNA revealed a significant similarity to a serine/threonine-protein kinase at the amino acid level (56% identity for 123 amino acids) and may represent a previously unknown kinase. Differential screening techniques coupled with single-pass cDNA sequence analysis may prove to be a powerful and simple technique to examine developmental gene expression
Promoting Teamwork in Translational Medical Teams: Insights and Recommendations from Science and Practice
Translational medical teams are transdisciplinary, highly collaborative, and operate within dynamic environments to solve time-sensitive and complex problems. These teams are tasked with turning observations in the laboratory and clinic into effective interventions that improve the health of individuals and the public. The nature of the problems they seek to solve requires coordination among clinicians, scientists, and experts from various scientific disciplines. Characteristically, translational medical teams have complex compositions, structure, and pluralistic goals, which pose significant challenges and barriers to enacting effective teamwork, compromising team performance. Given these challenges, it is imperative to glean insights from teams research and the science of team science on how to execute efficacious teamwork. Consequently, the purpose of this paper is to discuss specific teamwork processes (i.e., trust, communication, self-correction, backup behavior, shared mental models, and conflict management) that are critical to translational medical team performance and offer mechanisms to better equip such teams. Utilizing a theoretical framework of transdisciplinary teamwork adapted from the science of team science and tailored to translational medical teams, we describe each of these processes, their relation to translational medical team outcomes, and how they can be leveraged to improve teamwork. Such a discussion aims to provide practical guidance for conceptualizing and enhancing teamwork in translational medical teams
Real-time sweat pH monitoring based on a wearable chemical barcode micro-fluidic platform incorporating ionic liquids
This work presents the fabrication, characterisation and the performance of a wearable, robust, flexible
and disposable chemical barcode device based on a micro-fluidic platform that incorporates ionic
liquid polymer gels (ionogels). The device has been applied to the monitoring of the pH of sweat in
real time during an exercise period. The device is an ideal wearable sensor for measuring the pH of
sweat since it does not contents any electronic part for fluidic handle or pH detection and because it can
be directly incorporated into clothing, head- or wristbands, which are in continuous contact with the
skin. In addition, due to the micro-fluidic structure, fresh sweat is continuously passing through the sensing area providing the capability to perform continuous real time analysis. The approach presented here ensures immediate feedback regarding sweat composition. Sweat analysis is attractive for monitoring purposes as it can provide physiological information directly relevant to the health and performance of the wearer without the need for an invasive sampling approac
Methods of Inactivation of SARS-CoV-2 for Downstream Biological Assays
The scientific community has responded to the coronavirus disease 2019 (COVID-19) pandemic by rapidly undertaking research to find effective strategies to reduce the burden of this disease. Encouragingly, researchers from a diverse array of fields are collectively working towards this goal. Research with infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is undertaken in high-containment laboratories; however, it is often desirable to work with samples at lower-containment levels. To facilitate the transfer of infectious samples from high-containment laboratories, we have tested methods commonly used to inactivate virus and prepare the sample for additional experiments. Incubation at 80°C, a range of detergents, Trizol reagents, and UV energies were successful at inactivating a high titer of SARS-CoV-2. Methanol and paraformaldehyde incubation of infected cells also inactivated the virus. These protocols can provide a framework for in-house inactivation of SARS-CoV-2 in other laboratories, ensuring the safe use of samples in lower-containment levels
Second harmonic generation and birefringence of some ternary pnictide semiconductors
A first-principles study of the birefringence and the frequency dependent
second harmonic generation (SHG) coefficients of the ternary pnictide
semiconductors with formula ABC (A = Zn, Cd; B = Si, Ge; C = As, P) with
the chalcopyrite structures was carried out. We show that a simple empirical
observation that a smaller value of the gap is correlated with larger value of
SHG is qualitatively true. However, simple inverse power scaling laws between
gaps and SHG were not found. Instead, the real value of the nonlinear response
is a result of a very delicate balance between different intraband and
interband terms.Comment: 13 pages, 12 figure
BLAST: The Mass Function, Lifetimes, and Properties of Intermediate Mass Cores from a 50 Square Degree Submillimeter Galactic Survey in Vela (l = ~265)
We present first results from an unbiased 50 deg^2 submillimeter Galactic
survey at 250, 350, and 500 micron from the 2006 flight of the Balloon-borne
Large Aperture Submillimeter Telescope (BLAST). The map has resolution ranging
from 36 arcsec to 60 arcsec in the three submillimeter bands spanning the
thermal emission peak of cold starless cores. We determine the temperature,
luminosity, and mass of more than one thousand compact sources in a range of
evolutionary stages and an unbiased statistical characterization of the
population. From comparison with C^(18)O data, we find the dust opacity per gas
mass, kappa r = 0.16 cm^2 g^(-1) at 250 micron, for cold clumps. We find that
2% of the mass of the molecular gas over this diverse region is in cores colder
than 14 K, and that the mass function for these cold cores is consistent with a
power law with index alpha = -3.22 +/- 0.14 over the mass range 14 M_sun < M <
80 M_sun. Additionally, we infer a mass-dependent cold core lifetime of t_c(M)
= 4E6 (M/20 M_sun)^(-0.9) years - longer than what has been found in previous
surveys of either low or high mass cores, and significantly longer than free
fall or likely turbulent decay times. This implies some form of non-thermal
support for cold cores during this early stage of star formation.Comment: Accepted for publication in the Astrophysical Journal. Maps available
at http://blastexperiment.info
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