The city inhabits me: space, topology, and Gabriele Basilico’s Milan: Ritratti di Fabbriche

The 1975 New Topographics exhibition has been inscribed into photographic history as a starting point to which nearly all visually cognate practices can be traced back. This has held back more subtle and nuanced readings of much English and European work of the same era, particularly in the English-language press. Set in a more extended historical and geographical context, the work in the exhibition can be understood as part of a wider process of photographic exploration that took place alongside shifting patterns of production and consumption that transformed the global landscape in the decades following World War II. The exhibition also set out a specific position regarding the nature of topographic photography itself. Although New Topographics did not take an explicitly critical stance vis-a-vis landscape, one of its most enduring legacies has been the emergence of a ‘new topographics’ aesthetic that is understood as critically engaged simply by virtue of its distanced, deadpan style. To argue that particular photographers work in the topographic mode is thus to overlook the socio-political and geographical specificities of the places they represent, in favour of formal similarities. This paper examines Gabriele Basilico’s first project – Milan: Ritratti di Fabbriche (1978-80) – the photographs themselves, the context out of which they emerged, their presentation in book form, and Basilico’s own approach to the environments in which he photographed. As I argue, Milan: Ritratti di Fabbriche shares less than we might assume with the New Topographics work. Rather, it embodies a way of understanding and representing space as topological: heterogeneous and fluid, composed of multiple and often contradictory objects, processes and agents

The Feminine Awkward: Graceless Bodies and the Performance of Femininity in Fashion Photographs

Recent fashion photography has been preoccupied with awkward and uncomfortable poses. In fact, awkwardness – a negative affect comprising emotional and bodily discomfort – is part of the history of fashion photography. The ‘feminine awkward’ is proposed here as a formal and critical idiom for unpacking the relations that exist between the model, the photographic frame, and the camera, and for considering the way that the image is experienced by the viewer. The meaning of awkward fashion photographs is not only communicated directly, through various signifying practices; it also arises out of deeper emotional and bodily responses that accompany signification. The feminine awkward emerges most openly into fashion images during periods when gender norms are most forcefully contested, interfering with the trajectories of desire to which fashion images typically give rise. Culturally, it is aligned with paradigm shifts in social attitudes: with changing expectations of how a feminine subject should look and she should act. Looking at fashion photographs through the optic of the feminine awkward opens up new ways of thinking about the way that fashion photography simultaneously participates in, and unsettles, the production of gendered bodies

Determination of phototropism by UV-B radiation

UV-B phototropism in etiolated Arabidopsis seedlings has only been shown recently and needs further exploration. Here we elaborate on how to generate a customized setup with a unilateral UV-B light source, the required plant materials, different growth substrates, and a framework for data analysis

Empathy at Play:Embodying Posthuman Subjectivities in Gaming

In this article, we address the need for a posthuman account of the relationship between the avatar and player. We draw on a particular line of posthumanist theory associated closely with the work of Karen Barad, Rosi Braidotti and N. Katherine Hayles that suggests a constantly permeable, fluid and extended subjectivity, displacing the boundaries between human and other. In doing so, we propose a posthuman concept of empathy in gameplay, and we apply this concept to data from the first author’s 18-month ethnographic field notes of gameplay in the MMORPG World of Warcraft. Exploring these data through our analysis of posthuman empathy, we demonstrate the entanglement of avatar–player, machine–human relationship. We show how empathy allows us to understand this relationship as constantly negotiated and in process, producing visceral reactions in the intra-connected avatar–player subject as well as moments of co-produced in-game action that require ‘affective matching’ between subjective and embodied experiences. We argue that this account of the avatar–player relationship extends research in game culture, providing a horizontal, non-hierarchical discussion of its most necessary interaction

Epigenetic regulation during cancer transitions across 11 tumour types

Chromatin accessibility is essential in regulating gene expression and cellular identity, and alterations in accessibility have been implicated in driving cancer initiation, progression and metastasi

Combining the Tyrosine Kinase Inhibitor Cabozantinib and the mTORC1/2 Inhibitor Sapanisertib Blocks ERK Pathway Activity and Suppresses Tumor Growth in Renal Cell Carcinoma.

UNLABELLED: Current treatment approaches for renal cell carcinoma (RCC) face challenges in achieving durable tumor responses due to tumor heterogeneity and drug resistance. Combination therapies that leverage tumor molecular profiles could offer an avenue for enhancing treatment efficacy and addressing the limitations of current therapies. To identify effective strategies for treating RCC, we selected ten drugs guided by tumor biology to test in six RCC patient-derived xenograft (PDX) models. The multitargeted tyrosine kinase inhibitor (TKI) cabozantinib and mTORC1/2 inhibitor sapanisertib emerged as the most effective drugs, particularly when combined. The combination demonstrated favorable tolerability and inhibited tumor growth or induced tumor regression in all models, including two from patients who experienced treatment failure with FDA-approved TKI and immunotherapy combinations. In cabozantinib-treated samples, imaging analysis revealed a significant reduction in vascular density, and single-nucleus RNA sequencing (snRNA-seq) analysis indicated a decreased proportion of endothelial cells in the tumors. SnRNA-seq data further identified a tumor subpopulation enriched with cell-cycle activity that exhibited heightened sensitivity to the cabozantinib and sapanisertib combination. Conversely, activation of the epithelial-mesenchymal transition pathway, detected at the protein level, was associated with drug resistance in residual tumors following combination treatment. The combination effectively restrained ERK phosphorylation and reduced expression of ERK downstream transcription factors and their target genes implicated in cell-cycle control and apoptosis. This study highlights the potential of the cabozantinib plus sapanisertib combination as a promising treatment approach for patients with RCC, particularly those whose tumors progressed on immune checkpoint inhibitors and other TKIs. SIGNIFICANCE: The molecular-guided therapeutic strategy of combining cabozantinib and sapanisertib restrains ERK activity to effectively suppress growth of renal cell carcinomas, including those unresponsive to immune checkpoint inhibitors

Network centrality and organizational aspirations: A behavioral interaction in the context of international strategic alliances

Whereas social network analysis has been associated with organizational aspirations, little is known on how firm's structural positioning, and particularly network centrality, affects organizational aspirations to engage in international strategic alliances (ISA). This study examines the impact of network centrality on firm's internationalization behavior within the ISA domain in response to the performance-aspiration gap. We build on social and behavioral perspectives to predict that network centrality and performance-based aspirations will be associated with the number of ISA the firm engages in. Using a sample of 7760 alliance collaborations from the top 81 global pharmaceutical firms for the period of 1991-2012, we find supporting evidence for most of our arguments

Spatially restricted drivers and transitional cell populations cooperate with the microenvironment in untreated and chemo-resistant pancreatic cancer

Pancreatic ductal adenocarcinoma is a lethal disease with limited treatment options and poor survival. We studied 83 spatial samples from 31 patients (11 treatment-naïve and 20 treated) using single-cell/nucleus RNA sequencing, bulk-proteogenomics, spatial transcriptomics and cellular imaging. Subpopulations of tumor cells exhibited signatures of proliferation, KRAS signaling, cell stress and epithelial-to-mesenchymal transition. Mapping mutations and copy number events distinguished tumor populations from normal and transitional cells, including acinar-to-ductal metaplasia and pancreatic intraepithelial neoplasia. Pathology-assisted deconvolution of spatial transcriptomic data identified tumor and transitional subpopulations with distinct histological features. We showed coordinated expression of TIGIT in exhausted and regulatory T cells and Nectin in tumor cells. Chemo-resistant samples contain a threefold enrichment of inflammatory cancer-associated fibroblasts that upregulate metallothioneins. Our study reveals a deeper understanding of the intricate substructure of pancreatic ductal adenocarcinoma tumors that could help improve therapy for patients with this disease