Dynamics of solar wind protons reflected by the Moon

Solar system bodies that lack a significant atmosphere and significant internal magnetic fields, such as the Moon and asteroids, have been considered as passive absorbers of the solar wind. However, ion observations near the Moon by the SELENE spacecraft show that a fraction of the impacting solar wind protons are reflected by the surface of the Moon. Using new observations of the velocity spectrum of these reflected protons by the SARA experiment on-board the Chandrayaan-1 spacecraft at the Moon, we show by modeling that the reflection of solar wind protons will affect the global plasma environment. These global perturbations of the ion fluxes and the magnetic fields will depend on microscopic properties of the object's reflecting surface. This solar wind reflection process could explain past ion observations at the Moon, and the process should occur universally at all atmosphereless non-magnetized objects.Comment: 12 pages, 8 figure

A Case Study of Retro Commissioning in a Standard Commercial Office Building in Japan

This paper describes retro commissioning of a standard commercial office building in Japan. The owner's expectations for retro commissioning are realization of energy and cost savings, and controlling the increase in electric power demand, while continuing use of most existing equipment, and maintenance of high efficiency operation. First, the performance of the existing equipment was checked using the BEMS during retro commissioning program phase. Next, optimal selection of a system and heat source equipment was performed using the simulation in the design phase. Furthermore, the verification of the performance of the refrigeration machine installed was carried out as a Functional Performance Test. And the economic effect by a repair work was verified during the operation phase. The simple payback of the project was about six years

Quantum Effects in Small-Capacitance Single Josephson Junctions

We have measured the current-voltage (I-V) characteristics of small-capacitance single Josephson junctions at low temperatures (T=0.02-0.6 K), where the strength of the coupling between the single junction and the electromagnetic environment was controlled with one-dimensional arrays of dc SQUIDs. The single-junction I-V curve is sensitive to the impedance of the environment, which can be tuned IN SITU. We have observed Coulomb blockade of Cooper-pair tunneling and even a region of negative differential resistance, when the zero-bias resistance R_0' of the SQUID arrays is much higher than the quantum resistance R_K = h/e^2 = 26 kohm. The negative differential resistance is evidence of coherent single-Cooper-pair tunneling within the theory of current-biased single Josephson junctions. Based on the theory, we have calculated the I-V curves numerically in order to compare with the experimental ones at R_0' >> R_K. The numerical calculation agrees with the experiments qualitatively. We also discuss the R_0' dependence of the single-Josephson-junction I-V curve in terms of the superconductor-insulator transition driven by changing the coupling to the environment.Comment: 11 pages with 14 embedded figures, RevTeX4, final versio

Export of functional Streptomyces coelicolor alditol oxidase to the periplasm or cell surface of Escherichia coli and its application in whole-cell biocatalysis

Streptomyces coelicolor A3(2) alditol oxidase (AldO) is a soluble monomeric flavoprotein in which the flavin cofactor is covalently linked to the polypeptide chain. AldO displays high reactivity towards different polyols such as xylitol and sorbitol. These characteristics make AldO industrially relevant, but full biotechnological exploitation of this enzyme is at present restricted by laborious and costly purification steps. To eliminate the need for enzyme purification, this study describes a whole-cell AldO biocatalyst system. To this end, we have directed AldO to the periplasm or cell surface of Escherichia coli. For periplasmic export, AldO was fused to endogenous E. coli signal sequences known to direct their passenger proteins into the SecB, signal recognition particle (SRP), or Twin-arginine translocation (Tat) pathway. In addition, AldO was fused to an ice nucleation protein (INP)-based anchoring motif for surface display. The results show that Tat-exported AldO and INP-surface-displayed AldO are active. The Tat-based system was successfully employed in converting xylitol by whole cells, whereas the use of the INP-based system was most likely restricted by lipopolysaccharide LPS in wild-type cells. It is anticipated that these whole-cell systems will be a valuable tool for further biological and industrial exploitation of AldO and other cofactor-containing enzymes.

The integrin inhibitor cilengitide enhances the anti-glioma efficacy of vasculostatin-expressing oncolytic virus

Oncolytic viral (OV) therapy has been considered as a promising treatment modality for brain tumors. Vasculostatin, the fragment of brain-specific angiogenesis inhibitor-1, shows anti-angiogenic activity against malignant gliomas. Previously, a vasculostatin-expressing oncolytic herpes simplex virus-1, Rapid Antiangiogenesis Mediated By Oncolytic virus (RAMBO), was reported to have a potent antitumor effect. Here, we investigated the therapeutic efficacy of RAMBO and cilengitide, an integrin inhibitor, combination therapy for malignant glioma. In vitro, tube formation was significantly decreased in RAMBO and cilengitide combination treatment compared with RAMBO or cilengitide monotherapy. Moreover, combination treatment induced a synergistic suppressive effect on endothelial cell migration compared with the control virus. RAMBO, combined with cilengitide, induced synergistic cytotoxicity on glioma cells. In the caspase-8 and -9 assays, the relative absorption of U87 Delta EGFR cell clusters treated with cilengitide and with RAMBO was significantly higher than that of those treated with control. In addition, the activity of caspase 3/7 was significantly increased with combination therapy. In vivo, there was a significant increase in the survival of mice treated with combination therapy compared with RAMBO or cilengitide monotherapy. These results indicate that cilengitide enhanced vasculostatin-expressing OV therapy for malignant glioma and provide a rationale for designing future clinical trials combining these two agents

Expansion of magnetic clouds in the outer heliosphere

A large amount of magnetized plasma is frequently ejected from the Sun as coronal mass ejections (CMEs). Some of these ejections are detected in the solar wind as magnetic clouds (MCs) that have flux rope signatures. Magnetic clouds are structures that typically expand in the inner heliosphere. We derive the expansion properties of MCs in the outer heliosphere from one to five astronomical units to compare them with those in the inner heliosphere. We analyze MCs observed by the Ulysses spacecraft using insitu magnetic field and plasma measurements. The MC boundaries are defined in the MC frame after defining the MC axis with a minimum variance method applied only to the flux rope structure. As in the inner heliosphere, a large fraction of the velocity profile within MCs is close to a linear function of time. This is indicative of} a self-similar expansion and a MC size that locally follows a power-law of the solar distance with an exponent called zeta. We derive the value of zeta from the insitu velocity data. We analyze separately the non-perturbed MCs (cases showing a linear velocity profile almost for the full event), and perturbed MCs (cases showing a strongly distorted velocity profile). We find that non-perturbed MCs expand with a similar non-dimensional expansion rate (zeta=1.05+-0.34), i.e. slightly faster than at the solar distance and in the inner heliosphere (zeta=0.91+-0.23). The subset of perturbed MCs expands, as in the inner heliosphere, at a significantly lower rate and with a larger dispersion (zeta=0.28+-0.52) as expected from the temporal evolution found in numerical simulations. This local measure of the expansion also agrees with the distribution with distance of MC size,mean magnetic field, and plasma parameters. The MCs interacting with a strong field region, e.g. another MC, have the most variable expansion rate (ranging from compression to over-expansion)

The change of plasma C-reactive protein and metabolite concentrations, and MPS sick degree score in Landrase selected for resistance to MPS, Large Yorkshire selected for immune performances and the crossbreed

Swine Mycoplasma Hyopneumoniea, hp, is known as a major factor to affect for the specific pneumonia (MPS). This damages is very serious because carrier rate of hp in piglets from 3 to 4 months of age is very high, the rate of piglets that the response of antibody to hp shows positive is 80 % over, and the rate that has very terrible tissue from MPS is 51% in Japanese pig farm. We bred a resistant strain to MPS by selection to decrease MPS pathogenic condition over 5 generations using Landrase (MPS strain), and a high immune performance strain by selection for peripheral phagocytosis, complement activity and antibody production against erysipelatous vaccine using Large Yorkshire (HI strain)