Food Safety Knowledge and Practice Among Community in Sg. Pelek, Sepang, Selangor Darul Ehsan

Food safety is a basic requirement of food quality. It is an increasingly important public health issue to prevent and control food borne illnesses. A cross-sectional study was designed to determine level of knowledge and practice on food safety, to determine the association between knowledge and practice, and also to identify the association between socio-demographic factors and practice score of the population studied. This study was conducted among adult population in Taman Bahagia, Sungai Pelek, Sepang, Selangor Darul Ehsan. Data were collected using an interviewed structured questionnaire. A stratified random sampling was performed to obtain houses, followed by simple random sampling to select sample in the house. A total of 115 data sets were analysed using Statistical Package for Social Sciences (SPSS) version 20.0. Analysis showed that 35% of respondents have poor level of knowledge on food safety, whereas 27% of the respondents have poor level of practices on food safety. Multiple linear regression revealed that there are a significant association between education level (p<0.001), Adj b=2.57 (95% CI: 1.15, 3.99) and gender (p=0.048), Adj b=1.15 (95% CI: 0.01, 2.29) with practice score on food safety. Therefore, health promotion and education on the importance of practicing food safety at home should be focused to prevent further unwanted health effects

Production of the neutral top-pion πt0\pi_{t}^{0} in association with a high-pTp_{T} jet at the LHCLHC

In the framework of the topcolor-assisted technicolor $(TC2)$ model, we study production of the neutral top-pion $\pi_{t}^{0}$ in association with a high-$p_{T}$ jet at the $LHC$, which proceeds via the partonic processes $gg\longrightarrow \pi_{t}^{0}g$, $gq\longrightarrow \pi_{t}^{0}q$, $q\bar{q}\longrightarrow \pi_{t}^{0}g$, $gb(\bar{b})\longrightarrow \pi_{t}^{0}b(\bar{b})$, and $b\bar{b}\longrightarrow \pi_{t}^{0}g$. We find that it is very challenging to detect the neutral top-pion $\pi_{t}^{0}$ via the process $pp\longrightarrow \pi_{t}^{0}+jet+X\to t\bar{t}+jet+X$, while the possible signatures of $\pi_{t}^{0}$ might be detected via the process $pp\longrightarrow \pi_{t}^{0}+jet+X\to(\bar{t}c+t\bar{c})+jet+X$ at the $LHC$.Comment: 13 pages, 4 figures; typos correcte

The microRNA-29 family in cartilage homeostasis and osteoarthritis

MicroRNAs have been shown to function in cartilage development and homeostasis, as well as in progression of osteoarthritis. The objective of the current study was to identify microRNAs involved in the onset or early progression of osteoarthritis and characterise their function in chondrocytes. MicroRNA expression in mouse knee joints post-DMM surgery was measured over 7 days. Expression of miR-29b-3p was increased at day 1 and regulated in the opposite direction to its potential targets. In a mouse model of cartilage injury and in end-stage human OA cartilage, the miR-29 family were also regulated. SOX9 repressed expression of miR-29a-3p and miR-29b-3p via the 29a/b1 promoter. TGFβ1 decreased expression of miR-29a, b and c (3p) in primary chondrocytes, whilst IL-1β increased (but LPS decreased) their expression. The miR-29 family negatively regulated Smad, NFκB and canonical WNT signalling pathways. Expression profiles revealed regulation of new WNT-related genes. Amongst these, FZD3, FZD5, DVL3, FRAT2, CK2A2 were validated as direct targets of the miR-29 family. These data identify the miR-29 family as microRNAs acting across development and progression of OA. They are regulated by factors which are important in OA and impact on relevant signalling pathways

Adaptation of High-Growth Influenza H5N1 Vaccine Virus in Vero Cells: Implications for Pandemic Preparedness

Current egg-based influenza vaccine production technology can't promptly meet the global demand during an influenza pandemic as shown in the 2009 H1N1 pandemic. Moreover, its manufacturing capacity would be vulnerable during pandemics caused by highly pathogenic avian influenza viruses. Therefore, vaccine production using mammalian cell technology is becoming attractive. Current influenza H5N1 vaccine strain (NIBRG-14), a reassortant virus between A/Vietnam/1194/2004 (H5N1) virus and egg-adapted high-growth A/PR/8/1934 virus, could grow efficiently in eggs and MDCK cells but not Vero cells which is the most popular cell line for manufacturing human vaccines. After serial passages and plaque purifications of the NIBRG-14 vaccine virus in Vero cells, one high-growth virus strain (Vero-15) was generated and can grow over 108 TCID50/ml. In conclusion, one high-growth H5N1 vaccine virus was generated in Vero cells, which can be used to manufacture influenza H5N1 vaccines and prepare reassortant vaccine viruses for other influenza A subtypes

An integrated map of structural variation in 2,504 human genomes

Structural variants are implicated in numerous diseases and make up the majority of varying nucleotides among human genomes. Here we describe an integrated set of eight structural variant classes comprising both balanced and unbalanced variants, which we constructed using short-read DNA sequencing data and statistically phased onto haplotype blocks in 26 human populations. Analysing this set, we identify numerous gene-intersecting structural variants exhibiting population stratification and describe naturally occurring homozygous gene knockouts that suggest the dispensability of a variety of human genes. We demonstrate that structural variants are enriched on haplotypes identified by genome-wide association studies and exhibit enrichment for expression quantitative trait loci. Additionally, we uncover appreciable levels of structural variant complexity at different scales, including genic loci subject to clusters of repeated rearrangement and complex structural variants with multiple breakpoints likely to have formed through individual mutational events. Our catalogue will enhance future studies into structural variant demography, functional impact and disease association. © 2015 Macmillan Publishers Limited. All rights reserved

Publisher Correction: Stroke genetics informs drug discovery and risk prediction across ancestries (Nature, (2022), 611, 7934, (115-123), 10.1038/s41586-022-05165-3)

In the version of this article initially published, the name of the PRECISE4Q Consortium was misspelled as “PRECISEQ” and has now been amended in the HTML and PDF versions of the article. Further, data in the first column of Supplementary Table 55 were mistakenly shifted and have been corrected in the file accompanying the HTML version of the article

Negative feedback regulation of the ERK1/2 MAPK pathway

The extracellular signal-regulated kinase 1/2 (ERK1/2) mitogen-activated protein kinase (MAPK) signalling pathway regulates many cellular functions, including proliferation, differentiation, and transformation. To reliably convert external stimuli into specific cellular responses and to adapt to environmental circumstances, the pathway must be integrated into the overall signalling activity of the cell. Multiple mechanisms have evolved to perform this role. In this review, we will focus on negative feedback mechanisms and examine how they shape ERK1/2 MAPK signalling. We will first discuss the extensive number of negative feedback loops targeting the different components of the ERK1/2 MAPK cascade, specifically the direct posttranslational modification of pathway components by downstream protein kinases and the induction of de novo gene synthesis of specific pathway inhibitors. We will then evaluate how negative feedback modulates the spatiotemporal signalling dynamics of the ERK1/2 pathway regarding signalling amplitude and duration as well as subcellular localisation. Aberrant ERK1/2 activation results in deregulated proliferation and malignant transformation in model systems and is commonly observed in human tumours. Inhibition of the ERK1/2 pathway thus represents an attractive target for the treatment of malignant tumours with increased ERK1/2 activity. We will, therefore, discuss the effect of ERK1/2 MAPK feedback regulation on cancer treatment and how it contributes to reduced clinical efficacy of therapeutic agents and the development of drug resistance