Effect of pinching and spacing on growth, flowering and yield of African marigold (Tagetes erecta L.) under semi-arid conditions of Haryana

The present study was conducted at experimental orchard of Department of Horticulture, CCS Haryana Agricultural University, Hisar (Haryana), India with a view to optimize pinching time and spacing in African marigold for achieving better growth, flowering and yield. Design of the experiment was factorial Randomized Block Design with three replications. Experiment comprises of two levels of pinching (no pinching & pinching at 28 DAT i.e. Days After Transplanting) and three levels of spacing (40 x 40 cm, 40 x 30 cm & 30 x 30 cm) in all possible combinations. Maximum number of branches/plant (12.11), plant spread (63.59 cm), fresh weight of plant (358.79 g), number of buds/plant, duration of flowering (53.95 days), number of flowers/plant (54.54), stalk length and flower yield/plant (403.68 g) and flower yield/hectare (33.33 t) were significantly recorded in plants pinched at 28 DAT at 5% level of significance, whereas, maximum plant height (73.52 cm), flower diameter (6.38 cm), fresh weight of flower (8.93 g) was obtained in un-pinched plants. In case of spacing, maximum plant spread (64.01 cm), primary branches/plant (11.42), fresh weight of plant (370.20 g), number of buds/plant, number of flowers/plant (51.43), duration of flowering (53.98 days), flower diameter (6.42 cm), fresh weight of flower (9.03 g), flower yield/plant (460.42 g) was recorded at 40 x 40 cm, whereas, maximum plant height (71.76 cm), stalk length, flower yield/hectare (36.34 t) was observed with 30 x 30 cm. Results revealed that plants pinched at 28 DAT with widest spacing (40 x 40 cm) were found best for better growth, flowering and yield of African marigold

Mineralogy of soils of major geomorphic units of north-eastern Haryana, India

The study was carried to determine the mineralogy of soils of different geomorphic units for providing the more detailed information needed to improve agricultural production in north-eastern part of Haryana. The soils of the study area were slightly acidic to strongly alkaline in reaction (6-9.4). The cation exchange capacity and electrical conductivity varied from 3.10-26.80 cmol (+) kg-1 and 0.16-1.20 dSm-1, respectively. In general, the soils were siliceous in nature with SiO2 ranging from 68.60 to 87.90 percent. The soil samples from surface and subsurface diagnostic horizons were studied through X-ray diffraction. In fine sand, quartz was the dominant mineral followed by feldspars, muscovite, hornblende, tourmaline, zircon, biotite, iron ores and sphene. In silt fraction, quartz was the dominant mineral followed by mica, feldspars, chlorite, kaolinite, interstratified and traces of smectite and vermiculite. Semi-quantitative estimation of clay fraction indicated that illite was the single dominant mineral in the clay fraction of these pedons, however, its quantity was less in alluvial plains (28-30 %) compared to Shiwalik hills (36-49 %). Next to illite, a high amount of smectite (14-20 %) and vermiculite (11-17 %) were observed in clays of alluvial plains of Ghaggar (recent and old) whereas in Shiwalik hills (top and valley) these minerals were detected in small amount (6-11 %). Fairly good amount of kaolinite (10-17 %) and small amount of chlorite (4-11 %) were uniformly distributed in soil clays irrespective of geomorphic units showing their detrital origin. Medium intensity broad peaks in higher range diffractograms (14-24 AËš) indicated the presence of regular and irregular interstratified minerals in old alluvial plains of Ghaggar

Proteomic analysis of tomato (Lycopersicon esculentum) pollen

In flowering plants, pollen grains are produced in the anther and released to the external environment with the primary function of delivering sperm cells to the female gametophyte. This study was conducted to identify proteins in tomato pollen and to analyse their roles in relation to pollen function. Tomato is an important crop which is grown worldwide and is an excellent experimental system. Proteins were extracted from pollen, separated by two-dimensional gel electrophoresis (2-DE), and identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and peptide mass fingerprinting. Of the 960 spots observed on Colloidal Coomassie Blue (CCB)-stained 2-DE gels, 190 were selected for analysis. Of these, 158 spots, representing 133 distinct proteins, were identified by searching the NCBInr and Expressed Sequence Tag databases. The identified proteins were classified based on designated functions and the majority included those involved in defence mechanisms, energy conversions, protein synthesis and processing, cytoskeleton formation, Ca(2+) signalling, and as allergens. A number of proteins in tomato pollen were similar to those reported in the pollen of other species; however, several additional proteins with roles in defence mechanisms, metabolic processes, and hormone signalling were identified. The potential roles of the identified proteins in the survival strategy of the small, independent, two-celled pollen grain of tomato, and subsequently in pollen germination and tube growth are discussed

RS-based regional crop identification and mapping: A case study of Barwala sub-branch of Western Yamuna Canal in Haryana (India)

Information on spatial land use is the main input in strategic and tactical plan-making by all agricultural participants in countries like India with agriculture at the mainstay of the economy. In addition, accurately evaluation of the management of crops in a spatio-temporal context, information on cropping systems is also required, but such information on a regional scale are scarcely available. The spectrum of multiannual patterns of land use on cultivable land, however, remains unknown. The paper thus focuses on the mapping of the cropping systems that are actually practiced in Hisar District of Haryana (India). The objective of this research was to use satellite data and Remote Sensing (RS) techniques to identify the cropping pattern of Barwala sub-branch of Sirsa branch of the Western Yamuna Canal in Haryana. To identify classes of interest, handheld GPS was used to collect ground-truth information. Mask of mixed classes was developed to reclassify an image under the mask. Moreover, appropriate classification of images and application of logical combinations helped in generating cropping pattern maps and statistics. Results revealed that major crops identified in the study area were cotton, rice and pearl-millet in Kharif season, which accounts for about 70% of total cultivated area. In the case of Rabi season, wheat and mustard were observed as the major crops covering approximately 57% of total area. RS technology is currently capable of providing cropping pattern with 90% accuracy. The results of the current study could be useful in the land use and efficient water management in the canal command areas in the water-scarce southern Haryana, India. Some crops like guar, pearl-millet, horticultural crops, etc. were also identified during this period but the major crops that were identified during Kharif season were Rice and Cotton

Proteome profile and functional classification of proteins in Arabidopsis thaliana (Landsberg erecta) mature pollen

Proteome analysis of mature Arabidopsis thaliana (Landsberg erecta ecotype) pollen was conducted using two-dimensional gel electrophoresis and mass spectrometry. A total of 960 spots were resolved on pH 4–7 IPG strips and 110 distinct proteins were identified from 150 spots analyzed. The identified proteins were categorized based on their functional role in the pollen, which included proteins involved in energy regulation, defense-related mechanisms, calcium-binding and signaling, cytoskeletal formation, pollen allergens, glycine-rich proteins (GRPs), and late embryogenesis abundant (LEA) proteins. These proteins potentially play important roles in pollen function at maturity and during subsequent germination and tube growth. Some of the proteins identified were related to known pollen-specific transcripts, while some were similar to proteins found in the seed. In this study, 66 new proteins were identified which were not reported in two other recent studies on Arabidopsis pollen, 17 proteins were common in all three studies, and 35 or 26 proteins reported here had an overlap with one or the other two studies. These differences may be attributed to the methods of protein extraction, spot selection for analysis, and the ecotype used. Together, the three studies provide a broad spectrum of the Arabidopsis pollen proteome

Sensitivity Analysis for Decisive Design Parameters for Energy and Indoor Visual Performances of a Glazed Façade Office Building

The large size of a glazed component allows greater access to natural light inside and a wider view of the outdoors while protecting the inside from extreme weather conditions. However, glazed components make buildings energy inefficient compared to opaque components if not designed suitably, and sometimes they create glare discomforts too. In order to protect against excessive natural light and direct sunlight and for privacy, dynamic shading devices are integrated into the glazed façade. In this study, the impact of various glazing and shading design parameters has been investigated by performing uncertainty and sensitivity analyses. The uncertainty analysis indicates that the variance coefficients for the source energy use, lighting energy use, useful daylight illuminance (UDI), and shade-deployed time fraction are in the ranges of 15.04–30.47, 39.05–45.06, 40.57–49.92, and 19.35–52%, respectively. The dispersion in the energy and indoor visual performance is evident by the large variation in the source energy consumption and UDI (500–2000), which vary in the ranges of 250–450 kWh/(m2-year) and 5–90%. Furthermore, a sensitivity analysis identified the window-to-wall ratio (WWR), aspect ratio (ASR), glazing type (Gt), absorptance of the wall (Aw), and shade transmittance (ST) as major influences of the parameters. Each of the identified parameters has a different proportionate impact depending on the façade orientation and performance parameters

In vitro and in vivo protective efficacies of antibodies that neutralize the RNA N-glycosidase activity of Shiga toxin 2

<p>Abstract</p> <p>Backgound</p> <p>Shiga toxin 2 (Stx2), one of two Stx liberated by Stx-producing <it>Escherichia coli</it>, is composed of an A subunit monomer and a B subunit pentamer, and is directly linked with hemolytic uremic syndrome in children. The pentameric B subunit binds to its cell surface receptor Gb₃for toxin internalization, and the A subunit follows intracellular retrograde transport to the cytosol where its RNA <it>N</it>-glycosidase activity (RNA-NGA) shuts down the protein synthesis, and leads to cell death. The present study investigated the ability of 19 Stx2 A subunit-specific human monoclonal antibodies (HuMAbs) to neutralize the RNA-NGA, and the association this neutralizing activity with protection of HeLa cells and mice against Stx2-induced death.</p> <p>Results</p> <p>The HuMAbs that were stronger inhibitors of RNA-NGA were also better at neutralizing Stx2 mediated HeLa cell death, and those that were weaker inhibitors of RNA-NGA activity were also weaker in protecting HeLa cells. These results suggest that the ability of an A subunit-specific antibody to block the RNA-NGA of the toxin is directly related to its ability to neutralize Stx2-mediated HeLa cell death. However, with the exception of the best RNA-NGA blocking antibodies 5C12 and 2F10, the efficacies of antibody neutralization of RNA-NGA of Stx2 did not correlate with their <it>in vivo </it>protective efficacies. The HuMAb 6C3, which neutralized RNA N-glycosidase activity of Stx2 less effectively than the HuMAbs 6D8 and 6B7, protected 100% of the mice against Stx2 challenge at 50 μg/mouse dose. In contrast, the HuMAbs 6D8 and 6B7, which neutralized RNA N-glycosidase activity of Stx2 more effectively than 6C3, protected 20% and 0% mice at that dose, respectively.</p> <p>Conclusions</p> <p>The neutralization efficiency of the RNA-NGA of Stx2 by A subunit-specific antibodies correlate strongly with their abilities to protect HeLa cells against Stx2-mediated toxicity but only the strongest RNA-NGA-neutralizing antibodies correlate very well with both protecting HeLa cells and mice against Stx2 challenge.</p

Prospects for durable resistance against an old soybean enemy: a four-decade journey from Rpp1 (Resistance to Phakopsora pachyrhizi) to Rpp7

Open Access Journal; Published online: 1 July 2019Soybean rust (SBR), caused by Phakopsora spp., is a major global concern for soybean producers. SBR causing fungi are polycyclic and obligate biotrophs, rendering the study of their biology particularly tedious. Over the past four decades, substantial progress has been made towards understanding the epidemiology of the disease, the identification of sources of resistance, and the mapping of soybean loci conferring resistance to P. pachyrhizi (Rpp genes), since this species is particularly well established and widespread in many soybean growing areas. Although host-plant resistance is generally considered as the most desirable solution from an environmental, economic, and social perspective, other disease control approaches such as agronomic practices and chemical application are also important, and influence rust epidemiology as well as the durability of host plant resistance. This review focusses primarily on genetic aspects of SBR management and summarizes the research in the following areas: SBR symptoms, aetiology, pathogenic variation and population structure of Phakopsora populations, expression of soybean resistance to Phakopsora infection, genetics and molecular diagnostics of host resistance to pathogen, and resistance gene deployment approaches. Finally, the role of multidisciplinary strategies is discussed for achieving higher durability of SBR resistance in soybean

Growth analysis of fenugreek (Trigonella foenum-graecum L.) under various levels of farmyard manure and phosphorus

Field investigations were carried out during winter (rabi) season of 1996-97 and 1997-98 to study the effect of different levels of farmyard manure and phosphorus on growth of two fenugreek genotypes in sandy loam soils. The results indicated that genotype NLM was significantly superior to HM-65 in terms of plant height, LAI, LAD CGR and dry matter accumulation at all stages of crop growth, except at 30 DAS. These growth parameters in the genotype NLM resulted in higher seed yield. Plant height, LAI, LAD, CGR and dry matter accumulation were significantly higher with increase of FYM upto 15 t ha-1 at all stages of crop growth, except at 30 DAS. Seed yield was also enhanced significantly with the FYM up to 15 t ha-1. Increasing levels of phosphorus upto 40 kg ha-1 significantly increased the growth parameters and yield of fenugreek. &nbsp

Growth analysis of fenugreek (Trigonella foenum-graecum L.) under various levels of farmyard manure and phosphorus

Field investigations were carried out during winter (rabi) season of 1996-97 and 1997-98 to study the effect of different levels of farmyard manure and phosphorus on growth of two fenugreek genotypes in sandy loam soils. The results indicated that genotype NLM was significantly superior to HM-65 in terms of plant height, LAI, LAD CGR and dry matter accumulation at all stages of crop growth, except at 30 DAS. These growth parameters in the genotype NLM resulted in higher seed yield. Plant height, LAI, LAD, CGR and dry matter accumulation were significantly higher with increase of FYM upto 15 t ha-1 at all stages of crop growth, except at 30 DAS. Seed yield was also enhanced significantly with the FYM up to 15 t ha-1. Increasing levels of phosphorus upto 40 kg ha-1 significantly increased the growth parameters and yield of fenugreek. &nbsp