An evaluation of membrane properties and process characteristics of a scaled-up pressure retarded osmosis (PRO) process

YesThis work presents a systematic evaluation of the membrane and process characteristics of a scaled-up pressure retarded osmosis (PRO). In order to meet pre-defined membrane economic viability ( ≥ 5 W/m2), different operating conditions and design parameters are studied with respect to the increase of the process scale, including the initial flow rates of the draw and feed solution, operating pressure, membrane permeability-selectivity, structural parameter, and the efficiency of the high-pressure pump (HP), energy recovery device (ERD) and hydro-turbine (HT). The numerical results indicate that the performance of the scaled-up PRO process is significantly dependent on the dimensionless flow rate. Furthermore, with the increase of the specific membrane scale, the accumulated solute leakage becomes important. The membrane to achieve the optimal performance moves to the low permeability in order to mitigate the reverse solute permeation. Additionally, the counter-current flow scheme is capable to increase the process performance with a higher permeable and less selectable membrane compared to the co-current flow scheme. Finally, the inefficiencies of the process components move the optimal APD occurring at a higher dimensionless flow rate to reduce the energy losses in the pressurization and at a higher specific membrane scale to increase energy generation

Revealing the role of regulatory T cells in the tumor microenvironment of lung adenocarcinoma: a novel prognostic and immunotherapeutic signature

BackgroundRegulatory T cells (Tregs), are a key class of cell types in the immune system. In the tumor microenvironment (TME), the presence of Tregs has important implications for immune response and tumor development. Relatively little is known about the role of Tregs in lung adenocarcinoma (LUAD).MethodsTregs were identified using but single-cell RNA sequencing (scRNA-seq) analysis and interactions between Tregs and other cells in the TME were investigated. Next, we used multiple bulk RNA-seq datasets to construct risk models based on marker genes of Tregs and explored differences in prognosis, mutational landscape, immune cell infiltration and immunotherapy between high- and low-risk groups, and finally, qRT-PCR and cell function experiments were performed to validate the model genes.ResultsThe cellchat analysis showed that MIF-(CD74+CXCR4) pairs play a key role in the interaction of Tregs with other cell subpopulations, and the Tregs-associated signatures (TRAS) could well classify multiple LUAD cohorts into high- and low-risk groups. Immunotherapy may offer greater potential benefits to the low-risk group, as indicated by their superior survival, increased infiltration of immune cells, and heightened expression of immune checkpoints. Finally, the experiment verified that the model genes LTB and PTTG1 were relatively highly expressed in cancer tissues, while PTPRC was relatively highly expressed in paracancerous tissues. Colony Formation assay confirmed that knockdown of PTTG1 reduced the proliferation ability of LUAD cellsConclusionTRAS were constructed using scRNA-seq and bulk RNA-seq to distinguish patient risk subgroups, which may provide assistance in the clinical management of LUAD patients

Identification of rare DNA variants in mitochondrial disorders with improved array-based sequencing.

A common goal in the discovery of rare functional DNA variants via medical resequencing is to incur a relatively lower proportion of false positive base-calls. We developed a novel statistical method for resequencing arrays (SRMA, sequence robust multi-array analysis) to increase the accuracy of detecting rare variants and reduce the costs in subsequent sequence verifications required in medical applications. SRMA includes single and multi-array analysis and accounts for technical variables as well as the possibility of both low- and high-frequency genomic variation. The confidence of each base-call was ranked using two quality measures. In comparison to Sanger capillary sequencing, we achieved a false discovery rate of 2% (false positive rate 1.2 × 10⁻⁵, false negative rate 5%), which is similar to automated second-generation sequencing technologies. Applied to the analysis of 39 nuclear candidate genes in disorders of mitochondrial DNA (mtDNA) maintenance, we confirmed mutations in the DNA polymerase gamma POLG in positive control cases, and identified novel rare variants in previously undiagnosed cases in the mitochondrial topoisomerase TOP1MT, the mismatch repair enzyme MUTYH, and the apurinic-apyrimidinic endonuclease APEX2. Some patients carried rare heterozygous variants in several functionally interacting genes, which could indicate synergistic genetic effects in these clinically similar disorders

Emissions of volatile organic compounds (VOCs) from cooking and their speciation: A case study for Shanghai with implications for China

Cooking emission is one of sources for ambient volatile organic compounds (VOCs), which is deleterious to air quality, climate and human health. These emissions are especially of great interest in large cities of East and Southeast Asia. We conducted a case study in which VOC emissions from kitchen extraction stacks have been sampled in total 57 times in the Megacity Shanghai. To obtain representative data, we sampled VOC emissions from kitchens, including restaurants of seven common cuisine types, canteens, and family kitchens. VOC species profiles and their chemical reactivities have been determined. The results showed that 51.26% ± 23.87% of alkane and 24.33 ± 11.69% of oxygenated VOCs (O-VOCs) dominate the VOC cooking emissions. Yet, the VOCs with the largest ozone formation potential (OFP) and secondary organic aerosol potential (SOAP) were from the alkene and aromatic categories, accounting for 6.8–97.0% and 73.8–98.0%, respectively. Barbequing has the most potential of harming people's heath due to its significant higher emissions of acetaldehyde, hexanal, and acrolein. Methodologies for calculating VOC emission factors (EF) for restaurants that take into account VOCs emitted per person (EFperson), per kitchen stove (EFkitchen stove) and per hour (EFhour) are developed and discussed. Methodologies for deriving VOC emission inventories (S) from restaurants are further defined and discussed based on two categories: cuisine types (Stype) and restaurant scales (Sscale). The range of Stype and Sscale are 4124.33–7818.04 t/year and 1355.11–2402.21 t/year, respectively. We also found that Stype and Sscale for 100,000 people are 17.07–32.36 t/year and 5.61–9.95 t/year, respectively. Based on Environmental Kuznets Curve, the annual total amount of VOCs emissions from catering industry in different provinces in China was estimated, which was 5680.53 t/year, 6122.43 t/year, and 66,244.59 t/year for Shangdong and Guangdong provinces and whole China, respectively. Large and medium-scaled restaurants should be paid more attention with respect to regulation of VOCs

Controlled synthesis of monodisperse gold nanorods with different aspect ratios in the presence of aromatic additives

This paper reports the synthesis of monodisperse gold nanorods (GNRs) via a simple seeded growth approach in the presence of different aromatic additives, such as 7-bromo-3-hydroxy-2-naphthoic acid (7-BrHNA), 3-hydroxy-2-naphthoic acid (HNA), 5-bromosalicylic acid (5-BrSA), salicylic acid (SA) or phenol (PhOH). Effects of the aromatic additives and hydrochloric acid (HCl) on the structure and optical properties of the synthesized GNRs were investigated. The longitudinal surface plasmon resonance (LSPR) peak wavelength of the resulting GNRs was found to be dependent on the aromatic additive in the following sequence: 5-BrSA (778 nm) > 7-BrHNA (706 nm) > SA (688 nm) > HNA (676 nm) > PhOH (638 nm) without addition of HCl, but this was changed to 7-BrHNA (920 nm) > SA (890 nm) > HNA (872 nm) > PhOH (858 nm) > 5-BrSA (816 nm) or 7-BrHNA (1005 nm) > PhOH (995 nm) > SA (990 nm) > HNA (980 nm) > 5-BrSA (815 nm) with the addition of HCl or HNO3 respectively. The LSPR peak wavelength was increased with the increasing concentration of 7-BrHNA without HCl addition, however, there was a maximum LSPR peak wavelength when HCl was added. Interestingly, the LSPR peak wavelength was also increased with amount of HCl added. The results presented here thus established a simple approach to synthesize monodisperse GNRs of different LSPR wavelength

Functional study of SR splicing factors in a cellular genetic system

Pre-mRNA splicing is a critical and highly regulated process in eukaryotic gene expression. Splicing reaction is conducted by hundreds of protein factors, which form a structure called spliceosome. The SR protein family is a group of regulatory splicing factors whose functions have been well-studied in biochemical systems. Typical SR proteins contain one or two RNA recognition motifs at the N-terminus, and serine-arginine repeats at the C-terminus. SR proteins also play important roles in other steps of mRNA metabolism in the cell. Despite extensive biochemical characterization, the mechanism of SR protein action in the cellular context remains largely elusive. Inactivation of SR proteins by gene targeting indicates that the SR proteins are required for animal development and cell viability. However, the mechanism behind is far from clear. To study how SR proteins act in mammalian cells, we constructed two mouse embryo fibroblast (MEF) cell lines in which the endogenous genes for the SR protein ASF/SF2 and SC35 were each deleted and complemented by prospective exogenous genes expressed from a tetracycline-controlled promoter. Addition of tetracycline/ doxycycline in cell culture efficiently turns off the expression of these SR proteins. The inducible cell lines have been used as a genetic model in functional studies. Several interesting findings observed in these genetic systems form the basis of this dissertation: 1) In mammals, both SR proteins are uniquely required for survival of proliferating cells but dispensable in non-dividing cells. 2) RNA binding motif is required, while RS domain is disposable, for ASF/SF2 function in vivo. 3) Recycling SR proteins in cells is controlled by a sorting mechanism regulated by the phosphorylation state of RS domain. 4) SR proteins are critical for transcriptional elongation in mammalian cells. 5) The elongation defects trigger extensive double- stranded DNA breaks, leading to ATM-mediated activation of p53, inducing p21 expression, and cell cycle arrest. Our research has shed light on a number of fundamental questions regarding the function of SR proteins in regulated mRNA processing, maintenance of genomic stability and cell proliferation in vertebrates. Moreover, our work has established an experimental platform for future exploration of the cellular functions of SR protein

Explore the potential molecular mechanism of polycystic ovarian syndrome by protein–protein interaction network analysis

Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders prevailing in reproductive age women, present in 3–15% population of women worldwide. Although there are many studies on PCOS, its underlying mechanism remains to be determined. The present study was to construct protein–protein interaction networks based on the potential disease-causing genes for PCOS and characterize the underlying molecular mechanisms of PCOS using the networks. PCOS-associated genes were extracted from DisGeNet and the protein–protein interaction networks (PPIN) of PCOS were constructed using the String Database. Then we utilized MCODE algorithm to analyse the hub-gene modules from the PPIN. Finally, the major biological functions and signaling pathways involved in the hub modules were explored by functional enrichment analysis. A total of 522 candidate genes associated to PCOS were extracted from DisGeNET database. The PPIN constructed using the genes we have collected above included 488 genes and 2767 interaction relationships. Moreover, seven major gene modules were obtained after analyzing the PPIN with the use of MCODE plug-in. The major modules generated were enriched in certain biological functions such as cancer and cell proliferation and apoptosis, regulation of lipid and glucose metabolism, cell cycle and so on. The integrated analysis performed in the current study revealed that these hub modules and their related genes are closely associated to the pathogenesis of PCOS, which may probably provide novel insights for the treatment of PCOS and the study of its latent pathogenic mechanism. The relationship between several of the key genes including ALB, TOP2A, PTGER3, NPB and BRD2 in the modules and PCOS has not been investigated previously and it remains to be verified by further research of large sample, multi-center and multi-ethnic