As Redes de Museus como uma ferramenta de preservação do patrimônio cultural da Medicina no Brasil

SIAM. Series Iberoamericanas de Museología. Año 3, Vol.

Aumento de la fertilidad mediante técnicas de procesamiento avanzado de semen: caso clínico

Current equine reproductive clinic aims to improve subfertile stallions semen quality, (which is furthermore affected by the processing protocol) and maintain this quality during storage. The aim of this paper is to present a case referred to the Hospital Clínico Veterinario Complutense, of a stallion with low sperm quality, and 0% fertility rate in the first batch of mares served. Due to these results it was decided to perform a special semen processing protocol, developed by our laboratory, in order to increase its quality throughout a colloidal centrifugation technique with EquiPure® This system is able to increase semen quality and maintain itduring cooling preservation, obtaining results of around 57,1% of total fertility, which validates this technique as an effective method for subfertile stallions semen processing.La clínica reproductiva equina actual persigue mejorar la calidad seminal de sementales subfértiles, (la cual, además, se ve afectada por el procesamiento del semen) y que ésta se conserve durante el almacenamiento. El objetivo de este trabajo es presentar un caso clínico remitido al Hospital Clínico Veterinario Complutense, sobre un semental que presenta una baja calidad seminal, siendo la fertilidad del primer lote de yeguas del 0%. Ante estos resultados se decide realizar un procesado del semen, desarrollado por nuestro laboratorio, enfocado a aumentar su calidad mediante un protocolo de centrifugación coloidal con EquiPure® (Nidacon International AB). La aplicación de este protocolo consigue aumentar la calidad seminal y mantenerla durante la conservación en refrigeración, obteniéndose unos resultados de fertilidad total del 57,1%, lo que lo valida como método efectivo para el procesado del semen en casos de sementales subfértiles

Functional role of endothelial adhesion molecules in the early stages of brain metastasis

BACKGROUND: Cellular adhesion molecules (CAMs), which are normally associated with leukocyte trafficking, have also been shown to play an essential role in tumor metastasis to non-CNS sites. However, the role played by CAMs in brain metastasis is largely unexplored. It is known that leukocyte recruitment to the brain is very atypical and that mechanisms of disease in peripheral tissues cannot be extrapolated to the brain. Here, we have established the spatiotemporal expression of 12 key CAMs in the initial phases of tumor seeding in 2 different models of brain metastasis. METHODS: BALB/c or SCID mice were injected intracardially (10(5) cells/100 μL phosphate-buffered saline with either 4T1-GFP or MDA231BR-GFP cells, respectively (n = 4–6/group), and expression of the CAMs was determined by immunohistochemistry and immunofluorescence colocalisation. RESULTS: Endothelial expression of E-selectin, VCAM-1, ALCAM, ICAM-1, VLA-4, and β(4) integrin was markedly increased early in tumor seeding. At the same time, the natural ligands to these adhesion molecules were highly expressed on the metastatic tumor cells both in vitro and in vivo. Two of these ligands showed particularly high tumor cell expression (ALCAM and VLA-4), and consequently their functional role in tumor seeding was determined. Antibody neutralization of either ALCAM or VLA-4 significantly reduced tumor seeding within the brain (>60% decrease in tumor number/mm(2) brain; P < .05–0.01). CONCLUSIONS: These findings suggest that ALCAM/ALCAM and VLA-4/VCAM-1 interactions play an important functional role in the early stages of metastasis seeding in the brain. Moreover, this work identifies a specific subset of ligand-receptor interactions that may yield new therapeutic and diagnostic targets for brain metastasis

Memórias da Lepra: em busca de significados – contribuições para um debate

O texto pretende contribuir para o debate acerca da questão da memória dos ex-portadores de Lepra, abordados no artigo de Keila Auxiliadora Carvalho "Tempo de Lembrar: As Memórias dos Portadores de Lepra sobre o isolamento compulsório". Traz uma pequena revisão bibliográfica, situa o trabalho da autora e discute alguns pontos no intuito de apresentar questões que possam contribuir para aprofundar algumas análises sobre o tema.

Retreating to nature : rethinking 'therapeutic landscapes'

There is a long history of removing oneself from ‘society’ in order to recuperate or repair. This paper considers a yoga and massage retreat in Southern Spain, and what opportunities this retreat experience might offer for recuperation and the creation of healthy bodies. The paper positions ‘nature’ as an active participant, and as ‘enrolled’ in the experiences of the retreat as a ‘therapeutic landscape’, and questions how and what particular aspects of yoga practice (in intimate relation with place) give rise to therapeutic experiences