Watchdogs colaborativos para la detección de nodos maliciosos en redes MANET

En esta Tesis de Master se ha trabajado en la detección de nodos maliciosos que pudieran estar presentes en redes móviles ad-hoc (MANETs), utilizando watchdogs en los nodos que las forman, de tal manera que dichos watchdogs colaboran entre ellos para mejorar la rapidez y exactitud de la detección.Serrat Olmos, MD. (2011). Watchdogs colaborativos para la detección de nodos maliciosos en redes MANET. http://hdl.handle.net/10251/15673Archivo delegad

Maltrato económico hacia mujeres migrantes

Este Trabajo de Fin de Grado se basa en una investigación que tiene como finalidad averiguar si las mujeres migrantes tienen un mayor riesgo de sufrir maltrato económico, tanto en el marco laboral como en el familiar y de pareja, por razones de género y etnia. Se trata de un estudio cualitativo llevado a cabo a través de una exhaustiva documentación bibliográfica respaldada por un testimonio real.<br /

A novel Kluyveromyces marxianus strain with an inducible flocculation phenotype

Flocculation is a very useful phenotype for industrial yeast strains, since it facilitates cell harvest and represents an easy way of cell immobilization in continuous fermentation processes. The present work represents the first time that an inducible flocculation phenotype has been generated in a non flocculent strain of Kluyveromyces marxianus. This was accomplished by expressing Saccharomyces cerevisiae FLO5 gene in K. marxianus CECT 11769 strain. The FLO 5 gene was placed under the control of an EPG promoter, not repressed by glucose and induced by anoxia. Our experimental approach successfully generated two novel K. marxianus flocculent phenotypes: one inducible and one constitutive. The constitutive phenotype originated from deletions in the FLO5 promoter region, indicating the existence of putative upstream repressor site involved in oxygen regulation of the EPG1 promoter. The novel strains here generated had a unique set of characteristics that provided an advantage, over the wild-type strain, for the industrial co-production of ethanol and polygalacturonase.This work was supported by the AECID (Grant no. A/024951/09 and A/ 030029/10)S

Influence of nutritional and environmental factors on ethanol and endopolygalacturonase co-production by Kluyveromyces marxianus CCEBI 2011

Ethanol and endopolygalacturonase (endoPG) are simultaneously produced by the yeast Kluyveromyces marxianusCCEBI 2011. The aim of this study was to determine the optimal combination of seven environmental and nutritional variables, as well as the influence of each one, with respect to the fermentation process in yeast cultures in which sugarcanejuice was the substrate. Simplex sequential optimization showed that after 15 runs the optimal conditions were: pH, 4.6; temperature, 31&ordm;C; total reducing sugars (TRS), 125 g/l; (NH4)2SO4, 2.48 g/l; (NH4)2HPO4, 2.73 g/l; CaCl2, 0.33 g/l andMgSO4&middot;7H2O, 0.54 g/l. Under these conditions, the ethanol concentration was 47.6 g/l and endoPG concentration was 9.8 U/ml, which represented increases of 22% and 10%, respectively, over the concentrations obtained under suboptimal conditions. Temperature and (NH4)2SO4 supplementation were the most significant factors influencing the co-production process. [Int Microbiol 2011; 14(1):41-49

Combined production of electricity and hydrogen from solar energy and its use in the wine sector

© . This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/In the present research, the energy demanded by the wastewater treatment plant of a winery and the pumping station of the irrigation system of a vineyard is supplied by a stand-alone renewable energy system formed by three photovoltaic arrays connected to a microgrid. A relatively small battery maintains the stability and quality of the energy supply acting as a short-term energy storage. Hydrogen is generated in a production and refueling plant specifically designed for this project, and it is eventually used in a plug-in BEV properly modified as a hybrid vehicle by adding a PEM fuel cell. On the one hand, the technical and economic feasibility of the on-site electricity production for the winery and vineyard, compared to the commercial electricity from the grid and diesel gensets, is demonstrated. On the other hand, the diesel savings by the hydrogen generated on site are assessed. The electricity (72 MWh) and hydrogen (1,214 m3) produced in the first year have saved the emission of around 27 tons of equivalent CO2.Peer ReviewedPostprint (author's final draft