Le rôle de Ctso et d'Acta2, deux gènes candidats suppresseurs de métastase, dans la cascade métastatique

La métastase est la cause principale de décès chez les individus atteints de tumeurs solides. Lors d'un criblage pan-génomique d’ARN interférence, 22 gènes candidats suppresseurs de métastase ont été identifiés (Gobeil et al., 2008). Parmi ceux-ci, le gène de la cathepsine O (Ctso) et celui de l’actine alpha des muscles lisses (Acta2), ont été choisis pour être caractérisés. Les résultats obtenus en faisant des études de perte de fonction par ARN interférence et de gain de fonction via une expression ectopique, suggèrent que Ctso et Acta2 sont impliqués dans l’adhésion et la migration et potentiellement dans la survie/croissance cellulaire ainsi que dans l’invasion des cellules tumorales. De plus, mes résultats démontrent que la protéine Ctso est secrétée par des cellules de mélanome murin, suggérant une fonction extracellulaire. Bien que ces résultats tendent à démonter l’implication de Ctso et Acta2 dans la métastase, d’autres études doivent être accomplies pour approfondir leur rôle

PHYSICO-CHEMICAL AND BACTERIOLOGICAL ANALYSIS OF AUTOMOBILE SERVICE STATION RESIDUE WATERS

ABSTRACT The paper reports the analysis of service station residue water. In the service station used waters are mixed with fuel and other hydrocarbons related with. The collected service station water samples are analyzed for parameters, like Appearance and color, Turbidity , Total dissolved solids, Total hardness , Iron , Manganese, Fluoride, sulphate, phosphate ,TDS, pH, Electrical conductivity, Nitrate, calcium, Chloride, Magnesium, sodium and potassium also analyzed and the bacteriological count is also tested

www.jbino.com STUDIES ON PHYSICOCHEMICAL CHARACTERS OF FOUR PONDS IN TIRUNELVELI DISTRICT

The paper reports the analysis of pond water, which is the source of human consumption. The pond water is contaminated with the domestic wastes, Agricultural wastes and municipal wastes. The collected pond water samples are analyzed for parameters, like Appearance and color

Optimization of some fermentation conditions for the production of extracellular amylases by using Chryseobacterium and Bacillus isolates from organic kitchen wastes

Amylolytic bacterial isolates were obtained by starch-agar plate method from municipal solid wastes. Six amylolytic bacteria were isolated and the best two isolates, named as DY and W1, were selected based on clear zone ratio. The 16S rDNA sequence analysis identified DY and W1 isolates as Chryseobacterium sp. and Bacillus sp., respectively. Amylase production was optimized using basal media. The maximum level of amylase production was achieved from Chryseobacterium and Bacillus isolates after 60 h and 48 h of cultivation, respectively. The optimal temperature, initial pH of the media, agitation and inoculum size were determined for the both isolates. Increased amylase production was observed when basal media were substituted with organic carbon and nitrogen sources. The optimum pH and temperature for amylase activity of the crude amylase of Chryseobacterium sp. were 5.0 and 50 °C, respectively and those of amylase from Bacillus sp. were pH 7.0 and 50 °C, correspondingly. The crude amylase from the Chryseobacterium sp. was stable at pH 5.0–6.0 and up to 40 °C but that from Bacillus sp. was stable at pH 7.0 and up to 30 °C. Amylases from both the isolates lost ∼50% activity when stored at room temperature for two days. Under the optimized fermentation conditions both Chryseobacterium and Bacillus isolates produced almost the similar amount of amylase with organic kitchen wastes compared to the basal media. Results reported herein support the notion that Chryseobacterium sp. and Bacillus sp. can be used to produce industrially important amylases by utilizing organic kitchen wastes