Genetic variants in novel pathways influence blood pressure and cardiovascular disease risk.

Blood pressure is a heritable trait influenced by several biological pathways and responsive to environmental stimuli. Over one billion people worldwide have hypertension (≥140 mm Hg systolic blood pressure or  ≥90 mm Hg diastolic blood pressure). Even small increments in blood pressure are associated with an increased risk of cardiovascular events. This genome-wide association study of systolic and diastolic blood pressure, which used a multi-stage design in 200,000 individuals of European descent, identified sixteen novel loci: six of these loci contain genes previously known or suspected to regulate blood pressure (GUCY1A3-GUCY1B3, NPR3-C5orf23, ADM, FURIN-FES, GOSR2, GNAS-EDN3); the other ten provide new clues to blood pressure physiology. A genetic risk score based on 29 genome-wide significant variants was associated with hypertension, left ventricular wall thickness, stroke and coronary artery disease, but not kidney disease or kidney function. We also observed associations with blood pressure in East Asian, South Asian and African ancestry individuals. Our findings provide new insights into the genetics and biology of blood pressure, and suggest potential novel therapeutic pathways for cardiovascular disease prevention

Eco-friendly organic management of rust red flour beetle, Tribolium castaneum (Herbst) under stored conditions

The results showed that neem leaf powder was the most effective treatment with a record of the highest mean adult mortality of 67.22 per cent and also exhibited the lowest weight loss of 0.67 per cent after 60 days of treatment. It was followed by powders of Eucalyptus (58.89% & 0.87%) and Tulsi (51.11% & 0.80%). The least effectiveness of turmeric rhizome powder against the pest recording minimum adult mortality and maximum weight loss of 30.56% and 1.07% per cent respectively was noticed control against adult mortality (30.56 %), grain weight loss and adult emergence owing to T. castaneum. As a result, 1.5 g/100 g of grains of neem leaf powder can be recommended as a safe control method against T. castaneum in stored wheat. The overall mean adult mortality of T. castaneum in fenvalerate was 72.78 per cent. When it came to treating T. castneum with some inert materials, boric acid treatment was the most effective showed the 100 percent adult mortality, followed by coal ash (71.67 %) and cow dung ash (63.33 %) after 5 days of storage. There was minimum grain weight loss and least adult emergence was recorded in the treatment of coal ash and cow dung ash. Whereas sawdust resulted in the least effective treatment with mean adult mortality of (35.56 %), there was maximum adult emergence seen

Eco-friendly organic management of rust red flour beetle, Tribolium castaneum (Herbst) under stored conditions

The results showed that neem leaf powder was the most effective treatment with a record of the highest mean adult mortality of 67.22 per cent and also exhibited the lowest weight loss of 0.67 per cent after 60 days of treatment. It was followed by powders of Eucalyptus (58.89% & 0.87%) and Tulsi (51.11% & 0.80%). The least effectiveness of turmeric rhizome powder against the pest recording minimum adult mortality and maximum weight loss of 30.56% and 1.07% per cent respectively was noticed control against adult mortality (30.56 %), grain weight loss and adult emergence owing to T. castaneum. As a result, 1.5 g/100 g of grains of neem leaf powder can be recommended as a safe control method against T. castaneum in stored wheat. The overall mean adult mortality of T. castaneum in fenvalerate was 72.78 per cent. When it came to treating T. castneum with some inert materials, boric acid treatment was the most effective showed the 100 percent adult mortality, followed by coal ash (71.67 %) and cow dung ash (63.33 %) after 5 days of storage. There was minimum grain weight loss and least adult emergence was recorded in the treatment of coal ash and cow dung ash. Whereas sawdust resulted in the least effective treatment with mean adult mortality of (35.56 %), there was maximum adult emergence seen

Subchronic memantine induced concurrent functional disconnectivity and altered ultra-structural tissue integrity in the rodent brain: Revealed by multimodal MRI

Background: An effective NMDA antagonist imaging model may find key utility in advancing schizophrenia drug discovery research. We investigated effects of subchronic treatment with the NMDA antagonist memantine by using behavioural observation and multimodal MRI. Methods: Pharmacological MRI (phMRI) was used to map the neuroanatomical binding sites of memantine after acute and subchronic treatment. Resting state fMRI (rs-fMRI) and diffusion MRI were used to study the changes in functional connectivity (FC) and ultra-structural tissue integrity before and after subchronic memantine treatment. Further corroborating behavioural evidences were documented. Results: Dose-dependent phMRI activation was observed in the prelimbic cortex following acute doses of memantine. Subchronic treatment revealed significant effects in the hippocampus, cingulate, prelimbic and retrosplenial cortices. Decreases in FC amongst the hippocampal and frontal cortical structures (prelimbic, cingulate) were apparent through rs-fMRI investigation, indicating a loss of connectivity. Diffusion kurtosis MRI showed decreases in fractional anisotropy and mean diffusivity changes, suggesting ultra-structural changes in the hippocampus and cingulate cortex. Limited behavioural assessment suggested that memantine induced behavioural effects comparable to other NMDA antagonists as measured by locomotor hyperactivity and that the effects could be reversed by antipsychotic drugs. Conclusion: Our findings substantiate the hypothesis that repeated NMDA receptor blockade with nonspecific, noncompetitive NMDA antagonists may lead to functional and ultra-structural alterations, particularly in the hippocampus and cingulate cortex. These changes may underlie the behavioural effects. Furthermore, the present findings underscore the utility and the translational potential of multimodal MR imaging and acute/subchronic memantine model in the search for novel disease-modifying treatments for schizophrenia. \ua9 2013 Springer-Verlag Berlin Heidelberg