Interaction of Vascular Smooth Muscle Cells Under Low Shear Stress

The blood vessel wall consists of three cellular layers, an outer adventitial, a middle medial and an inner intimal layer. When the blood vessel forms in the embryo it begins as a tube composed of a single cell type called endothelial cells. Over time, other cells are recruited from the surrounding tissue to form additional layers on the outer surface of the endothelial tube. The cells that are recruited are called mesenchymal cells. Mesenchymal cells are responsible for the production of connective tissue that holds the blood vessel together and for developing into vascular smooth muscle cells that are responsible for regulating the diameter of the vessel (1) and therefore, blood flow. In a fully developed blood vessel, the endothelial cells make- up the majority of cells in the intimal layer while the mesenchymal cells make-up the majority of cells in the medial and adventitial layers. Within the medial layer of a mature vessel, cells are organized into multiple circular layers of alternating bands of connective tissue and cells. The cell layer is composed of a mixture of mesenchymal cells that have not developed into smooth muscle cells and fully developed smooth muscle cells (2). The assembly and organization of complex tissues is directed in part by a signaling system composed of proteins on the cell surface called adhesion molecules. Adhesion molecules enable cells to recognize each other as well as the composition of the connective tissue in which they reside (3). It was hypothesized that the different cell types that compose the vascular wall possess different adhesion molecules that enable them to recognize each other and through this recognition system, form the complex layered organization of the vascular wall. In other words, the layered organization is an intrinsic property of the cells. If this hypothesis is correct then the different cells that make up the vessel wall, when mixed together, should organize themselves into a layered structure resembling an intact blood vessel. Experiments described below were designed to test this hypothesis

Geometrical and structural properties of an Aeroelastic Research Wing (ARW-2)

Transonic steady and unsteady pressure tests were conducted on a large elastic wing known as the DAST ARW-2 wing. The wing has a supercritical airfoil, an aspect ratio of 10.3, a leading edge sweepback angle of 28.8 deg and is equipped with two inboard and one outboard trailing edge control surfaces. The geometrical and structural characteristics are presented of this elastic wing, using a combination of measured and calculated data, to permit future analyst to compare the experimental surface pressure data with theoretical predictions

Downregulation of FGF Signaling by Spry4 Overexpression Leads to Shape Impairment, Enamel Irregularities, and Delayed Signaling Center Formation in the Mouse Molar.

FGF signaling plays a critical role in tooth development, and mutations in modulators of this pathway produce a number of striking phenotypes. However, many aspects of the role of the FGF pathway in regulating the morphological features and the mineral quality of the dentition remain unknown. Here, we used transgenic mice overexpressing the FGF negative feedback regulator Sprouty4 under the epithelial keratin 14 promoter (K14-Spry4) to achieve downregulation of signaling in the epithelium. This led to highly penetrant defects affecting both cusp morphology and the enamel layer. We characterized the phenotype of erupted molars, identified a developmental delay in K14-Spry4 transgenic embryos, and linked this with changes in the tooth developmental sequence. These data further delineate the role of FGF signaling in the development of the dentition and implicate the pathway in the regulation of tooth mineralization. © 2019 The Authors. JBMR Plus is published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research

Space Station Control Moment Gyroscope Lessons Learned

Four 4760 Nms (3510 ft-lbf-s) Double Gimbal Control Moment Gyroscopes (DGCMG) with unlimited gimbal freedom about each axis were adopted by the International Space Station (ISS) Program as the non-propulsive solution for continuous attitude control. These CMGs with a life expectancy of approximately 10 years contain a flywheel spinning at 691 rad/s (6600 rpm) and can produce an output torque of 258 Nm (190 ft-lbf)1. One CMG unexpectedly failed after approximately 1.3 years and one developed anomalous behavior after approximately six years. Both units were returned to earth for failure investigation. This paper describes the Space Station Double Gimbal Control Moment Gyroscope design, on-orbit telemetry signatures and a summary of the results of both failure investigations. The lessons learned from these combined sources have lead to improvements in the design that will provide CMGs with greater reliability to assure the success of the Space Station. These lessons learned and design improvements are not only applicable to CMGs but can be applied to spacecraft mechanisms in general

Possible role of sodium-calcium pumps in tension development of vascular smooth muscle,

Observations were made on the tension development by helical strips of vascular smooth muscle from rabbit aorta and mesenteric artery. Based on the possibility that the pump which moves calcium into the cell coupled with sodium efflux, recently described for squid axon (1), exists also in vascular smooth muscle, studies were designed to evaluate changes in its activity in this muscle. Sodium competes with calcium for the carrier at the cell surface, hence the pump moves calcium into the cell more rapidly in a sodium-free medium. The strips developed more tension in a sodium-free solution, as if more ionized calcium were being delivered to the cell. Ouabain is known to block the sodium-potassium pump, thereby permitting an increase in sodium concentration inside the cell; this in turn will aceclerate the sodium-calcium pump. Ouabain potentiated the contractile responses of these muscles, suggesting that a higher concentration of ionized calcium is reaching the contractile protein.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/32889/1/0000268.pd

More Benefits of Semileptonic Rare B Decays at Low Recoil: CP Violation

We present a systematic analysis of the angular distribution of Bbar -> Kbar^\ast (-> Kbar pi) l^+ l^- decays with l = e, mu in the low recoil region (i.e. at high dilepton invariant masses of the order of the mass of the b-quark) to account model-independently for CP violation beyond the Standard Model, working to next-to-leading order QCD. From the employed heavy quark effective theory framework we identify the key CP observables with reduced hadronic uncertainties. Since some of the CP asymmetries are CP-odd they can be measured without B-flavour tagging. This is particularly beneficial for Bbar_s,B_s -> phi(-> K^+ K^-) l^+ l^- decays, which are not self-tagging, and we work out the corresponding time-integrated CP asymmetries. Presently available experimental constraints allow the proposed CP asymmetries to be sizeable, up to values of the order ~ 0.2, while the corresponding Standard Model values receive a strong parametric suppression at the level of O(10^-4). Furthermore, we work out the allowed ranges of the short-distance (Wilson) coefficients C_9,C_10 in the presence of CP violation beyond the Standard Model but no further Dirac structures. We find the Bbar_s -> mu^+ mu^- branching ratio to be below 9*10^-9 (at 95% CL). Possibilities to check the performance of the theoretical low recoil framework are pointed out.Comment: 18 pages, 3 fig.; 1 reference and comment on higher order effects added; EOS link fixed. Minor adjustments to Eqs 4.1-4.3 to match the (lower) q^2-cut as given in paper. Main results and conclusions unchanged; v3+v4: treatment of exp. uncert. in likelihood-function in EOS fixed and constraints from scan on C9,C10 updated (Fig 2,3 and Eqs 3.2,3.3). Main results and conclusions absolutely unchange

<i>Schizosaccharomyces pombe</i> Pol II transcription elongation factor ELL functions as part of a rudimentary super elongation complex

ELL family transcription factors activate the overall rate of RNA polymerase II (Pol II) transcription elongation by binding directly to Pol II and suppressing its tendency to pause. In metazoa, ELL regulates Pol II transcription elongation as part of a large multisubunit complex referred to as the Super Elongation Complex (SEC), which includes P-TEFb and EAF, AF9 or ENL, and an AFF family protein. Although orthologs of ELL and EAF have been identified in lower eukaryotes including Schizosaccharomyces pombe, it has been unclear whether SEClike complexes function in lower eukaryotes. In this report, we describe isolation from S. pombe of an ELL-containing complex with features of a rudimentary SEC. This complex includes S. pombe Ell1, Eaf1, and a previously uncharacterized protein we designate Ell1 binding protein 1 (Ebp1), which is distantly related to metazoan AFF family members. Like the metazoan SEC, this S. pombe ELL complex appears to function broadly in Pol II transcription. Interestingly, it appears to have a particularly important role in regulating genes involved in cell separation

The Benefits of B ---> K* l+ l- Decays at Low Recoil

Using the heavy quark effective theory framework put forward by Grinstein and Pirjol we work out predictions for B -> K* l+ l-, l = (e, mu), decays for a softly recoiling K*, i.e., for large dilepton masses sqrt{q^2} of the order of the b-quark mass m_b. We work to lowest order in Lambda/Q, where Q = (m_b, sqrt{q^2}) and include the next-to-leading order corrections from the charm quark mass m_c and the strong coupling at O(m_c^2/Q^2, alpha_s). The leading Lambda/m_b corrections are parametrically suppressed. The improved Isgur-Wise form factor relations correlate the B -> K* l+ l- transversity amplitudes, which simplifies the description of the various decay observables and provides opportunities for the extraction of the electroweak short distance couplings. We propose new angular observables which have very small hadronic uncertainties. We exploit existing data on B -> K* l+ l- distributions and show that the low recoil region provides powerful additional information to the large recoil one. We find disjoint best-fit solutions, which include the Standard Model, but also beyond-the-Standard Model ones. This ambiguity can be accessed with future precision measurements.Comment: 31 pages, 8 figures; Instability near minimal recoil from numerics removed, Fig. 1 replaced and minor shifts in short distance uncertainties in SM predictions; typos corrected and references added; main results and conclusions unchange

Correlação da qualidade da água com uso do solo e declividade no Arroio Doze Passos, Ouro, SC

Water is the main factor for the development and growth of communities, especially the rural ones. The objectiveof this study was to evaluate the relation of soil use and slope steepness with the water quality of the Arroio Doze Passos. Thisstudy was conducted in the watershed Arroio Doze Passos in the Midwest region of Santa Catarina State, Southern Brazil, fromNovember 2004 to October 2009. This watershed has an area of 17.78 km², where intensive agriculture is developed (mainlycorn and wheat) and livestock (mainly pigs, dairy cattle and poultry). It was observed that the stream water has low qualitydue the high values of total P, ortho P, turbidity, total coliform bacteria, and fecal coliforms. The main factor of poor waterquality is the presence of organic-origin manure, which is caused by land use out of its fitness ability, coupled with inadequatemanagement of waste, and lack of environmental planning. However, water quality, in general, improved from the stream’sspring to its mouth.A água é o principal fator para o desenvolvimento e crescimento das comunidades, especialmente das rurais. Este trabalho foi desenvolvido na microbacia Arroio Doze Passos, na região Meio-Oeste do Estado de Santa Catarina, no período de novembro de 2004 a outubro de 2009. O objetvo foi avaliar a relação do uso do solo e a declividade com a qualidade da águadesse arroio. Essa microbacia tem área de 17,78km², onde é desenvolvida intensa atvidade agrícola (principalmente lavouras de milho e trigo) e de criação de animais (principalmente suínos, gado leiteiro e aves). Observou-se que a água do arroio encontra-se com baixa qualidade devido aos altos teores de P total, P orto, turbidez e coliformes totais e fecais. A principalcausa da baixa qualidade da água é a presença de dejetos de origem orgânica, os quais são causados pela utlização do solo fora de sua capacidade de aptdão, pelo manejo inadequado dos dejetos e pela falta de planejamento paisagístco-ambiental. Entretanto, a qualidade da água, de forma geral, melhora da nascente para a foz