Evaluation of the effects of nitric oxide-releasing nanoparticles on plants

Nowadays, there are several commercially available products containing nanostructured materials. Meanwhile, despite the many benefits that can be obtained from nanotechnology, it is still necessary to understand the mechanisms in which nanomaterials interact with the environment, and to obtain information concerning their possible toxic effects. In agriculture, nanotechnology has been used in different applications, such as nanosensors to detect pathogens, nanoparticles as controlled release systems for pesticides, and biofilms to deliver nutrients to plants and to protect food products against degradation. Moreover, plants can be used as models to study the toxicity of nanoparticles. Indeed, phytotoxicity assays are required to identify possible negative effects of nanostructured systems, prior to their implementation in agriculture. Nitric oxide (NO) plays a key role in plant growth and defense, and recently, several papers described the beneficial effects due to application of exogenous NO donors in plants. The tripeptide glutathione (GSH) is an important anti-oxidant molecule and is the precursor of the NO donor, S-nitrosoglutathione (GSNO). In this context, the present work investigates the effects of different concentrations of alginate/chitosan nanoparticles, containing either GSH or GSNO, on the development of two test species (Zea mays and Glycine sp.). The results showed that the alginate/chitosan nanoparticles present a size average range from 300 to 550 nm with a polydispersity index of 0.35, and encapsulation efficiency of GSH between 45 - 56%. The NO release kinetics from the alginate/chitosan nanoparticles containing GSNO showed sustained and controlled NO release over several hours. Plant assays showed that at the concentrations tested (1, 5 and 10 mM of GSH or GSNO), polymeric nanoparticles showed no significant inhibitory effects on the development of the species Zea mays and Glycine sp., considering the variables shoot height, root length, and dry mass. Therefore, these nanoparticles seem to have promissing uses in agriculture, and might be potencially used as controlled release systems applied by the foliar route6174th International Conference on Safe Production and Use of Nanomaterials (Nanosafe

Application of Hydroinformatic Tools for Reservoirs Discharges Rules Assessment During a Flood Event

Hydrological and hydrodynamic models are valuable tools for understanding complex river hydrodynamics behavior during flood events. These tools have been applied to develop a detailed study of the flood event occurred between 9 and 11 January 2016 in the river Mondego basin, causing severe floods at Coimbra city (Portugal). The study included the characterization of the operational discharge schemes of three upstream dams with direct influence on flow rates in the river basin, and the runoff flows from contributing catchments. A detailed analysis on hydrodynamic water levels at the flooded areas influenced by the operation of a downstream dam and the local river morphodynamics was performed. Hydroinformatic tools were applied in different scenarios allowing the characterization and identification of the key factors responsible for the flood event and contributing to emphasize the need to comply with the established rules for the discharges at the upstream dams during flood eventsinfo:eu-repo/semantics/publishedVersio

Impact of Sediments and Constructions on River Flooding in Coimbra, Portugal

In Coimbra city, Portugal, the riverbanks have suffered several floods events in the past, due to its hydrological regime, the low slope and consequent lack of capacity of the Mondego River in its final 30 km. The construction of several dams in the upstream part of the river catchment has improved the use of the hydraulic capacity of the river system and reduced the number and intensity of flooding events in Coimbra. Nevertheless, intense rainfall events combined with inadequate procedures of the dam operation rules and lack of monitoring of sediments dynamics can still originate inundation in Coimbra such as those registered between 9th and 11th of January 2016. This work presents modelling scenarios demonstrating the influence of the sediment accumulation into the riverbed and its effect on the water levels. It also presents the influence that piers from a new bridge can have into the river flow dynamicsPortuguese Environmental Agency and the Portuguese National Association of Engineersinfo:eu-repo/semantics/publishedVersio

Genotype-phenotype correlations and BH4 estimated responsiveness in patients with phenylketonuria from Rio de Janeiro, Southeast Brazil

Background: Genetic heterogeneity and compound heterozygosis give rise to a continuous spectrum of phenylalanine hydroxylase deficiency and metabolic phenotypes in phenylketonuria (PKU). The most used parameters for evaluating phenotype in PKU are pretreatment phenylalanine (Phe) levels, tolerance for dietary Phe, and Phe overloading test. Phenotype can vary from a "classic" (severe) form to mild hyperphenylalaninemia, which does not require dietary treatment. A subset of patients is responsive to treatment by the cofactor tetrahydrobiopterin (BH4 ). Genotypes of PKU patients from Rio de Janeiro, Brazil, were compared to predicted and observed phenotypes. Genotype-based estimations of responsiveness to BH4 were also conducted. Methods: Phenotype was defined by pretreatment Phe levels. A standard prediction system based on arbitrary assigned values was employed to measure genotype-phenotype concordance. Patients were also estimated as BH4 -responders according to the responsiveness previously reported for their mutations and genotypes. Results: A 48.3% concordance rate between genotype-predicted and observed phenotypes was found. When the predicted phenotypes included those reported at the BIOPKU database, the concordance rate reached 77%. A total of 18 genotypes from 30 patients (29.4%) were estimated as of potential or probable BH4 responsiveness. Inconsistencies were observed in genotypic combinations including the common "moderate" mutations p.R261Q, p.V388M, and p.I65T and the mild mutations p.L48S, p.R68S, and p.L249F. Conclusion: The high discordance rate between genotype-predicted and observed metabolic phenotypes in this study seems to be due partially to the high frequency of the so-called "moderate" common mutations, p.R261Q, p.V388M, and p.I65T, which are reported to be associated to erratic or more severe than expected metabolic phenotypes. Although our results of BH4 estimated responsiveness must be regarded as tentative, it should be emphasized that genotyping and genotype-phenotype association studies are important in selecting patients to be offered a BH4 overload test, especially in low-resource settings like Brazil.Dr. Vieira Neto reports two public grants from Coordination for the Improvement of Higher Level Personnel (Capes) of the Ministry of Education, Brazil, and private grants from FBM Pharmaceutical Industry Ltd., Anápolis, Goiás, Brazil, and from Danone Ltd., São Paulo, Brazil, during the conduct of the study.info:eu-repo/semantics/publishedVersio

Dermoscopic features of nonpigmented eccrine poroma

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Unravelling the Affinity of Alkali-Activated Fly Ash Cubic Foams towards Heavy Metals Sorption

In this work, alkali-activated fly ash-derived foams were produced at room temperature by direct foaming using aluminum powder. The 1 cm3 foams (cubes) were then evaluated as adsorbents to extract heavy metals from aqueous solutions. The foams’ selectivity towards lead, cadmium, zinc, and copper ions was evaluated in single, binary, and multicomponent ionic solutions. In the single ion assays, the foams showed much higher affinity towards lead, compared to the other heavy metals; at 10 ppm, the removal efficiency reached 91.9% for lead, 83.2% for cadmium, 74.6% for copper, and 64.6% for zinc. The greater selectivity for lead was also seen in the binary tests. The results showed that the presence of zinc is detrimental to cadmium and copper sorption, while for lead it mainly affects the sorption rate, but not the ultimate removal efficiency. In the multicomponent assays, the removal efficiency for all the heavy metals was lower than the values seen in the single ion tests. However, the superior affinity for lead was preserved. This study decreases the existing knowledge gap regarding the potential of alkali-activated materials to act as heavy metals adsorbents under different scenarios

Home-based Rehabilitation With A Novel Digital Biofeedback System versus Conventional In-person Rehabilitation after Total Knee Replacement: a feasibility study

In-person home-based rehabilitation and telerehabilitation can be as effective as clinic-based rehabilitation after total knee arthroplasty (TKA), but require heavy logistics and are highly dependent on human supervision. New technologies that allow independent home-based rehabilitation without constant human supervision may help solve this problem. This was a single-center, feasibility study comparing a digital biofeedback system that meets these needs against conventional in-person home-based rehabilitation after TKA over an 8-week program. Primary outcome was the change in the Timed Up and Go score between the end of the program and baseline. Fifty-nine patients completed the study (30 experimental group; 29 conventional rehabilitation). The study demonstrated a superiority of the experimental group for all outcomes. Adverse events were similar in both groups. This is the first study to demonstrate that a digital rehabilitation solution can achieve better outcomes than conventional in-person rehabilitation, while less demanding in terms of human resources.his work was supported by the European Commission through the Project H2020 SME Instrument Phase 2 - Grant Agreement number 672814.info:eu-repo/semantics/publishedVersio

Introduction: Turnaround and let-down: making sense of Brazil and Africa after the surge

By improving political connections under a common South-South aegis, promoting new trade opportunities and expanding the disbursement of significant amounts of development cooperation, Brazil quickly secured a foothold of its own in Africa between 2003 and 2014. However, in the face of a political meltdown and of controversial judicial investigations back home, Brazil’s inversions in Africa have since then essentially collapsed. This abrupt turnaround calls for a more critical exegesis of the years of expansion. What were the main successes and failures of Brazil’s overall strategy towards Africa? And what does the dramatic change of events, with Brazil moving from a pivotal player to an almost invisible one in merely half a decade, tell us about the possibility of a new pick-up of interest for Africa? This introduction to the edited volume takes stock of the main trends in previous literature over the character and content of Brazil’s foreign policy towards the continent and sets the ground for the following chapters.info:eu-repo/semantics/acceptedVersio

From opportunity seeking to gap filling: Reframing Brazil in Lusophone Africa

This chapter inquires whether Brazil’s headways in Africa over recent years were organic in nature and in content or, in fact, were achieved at the expense of other previously established actors. By reframing Brazil’s agenda towards African lusophone countries in juxtaposition to the perceived external downturn of Portugal, the propitious context and consequences of a new player on the continent can be best brought into evidence. The push-and-pull forces enacted by both Brazil and Portugal towards Lusophone Africa are explored through the aftermath of the 2012 military coup in Guinea-Bissau and the adhesion of Equatorial Guinea to the Community of Portuguese Language Countries (CPLP) in 2014. The chapter offers a reinterpretation of Brazil’s net gains in Africa and argues for its fragility and susceptibility to changing political-economic cycles.info:eu-repo/semantics/acceptedVersio

Transcript of The Dory Derby Accident

This story is an excerpt from a longer interview that was collected as part of the Launching through the Surf: The Dory Fleet of Pacific City project. In this story, Don Grotjohn recounts an accident that occurred during a Dory Derby competition