Embryo futures and stem cell research: The management of informed uncertainty

This article is available open access and is distributed under a Creative Commons license (http://creativecommons.org/licenses/by/3.0/). Copyright @ 2011 The Authors.In the social worlds of assisted conception and stem cell science, uncertainties proliferate and particular framings of the future may be highly strategic. In this article we explore meanings and articulations of the future using data from our study of ethical and social issues implicated by the donation of embryos to human embryonic stem cell research in three linked assisted conception units and stem cell laboratories in the UK. Framings of the future in this field inform the professional management of uncertainty and we explore some of the tensions this involves in practice. The bifurcation of choices for donating embryos into accepting informed uncertainty or not donating at all was identified through the research process of interviews and ethics discussion groups. Professional staff accounts in this study contained moral orientations that valued ideas such as engendering patient trust by offering full information, the sense of collective ownership of the National Heath Service and publicly funded science and ideas for how donors might be able to give restricted consent as a third option.The Wellcome Trus

Donation of 'spare' fresh or frozen embryos to research: Who decides that an embryo is 'spare' and how can we enhance the quality and protect the validity of consent?

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited - Copyright @ The Author 2012.This paper analyses elements of the legal process of consent to the donation of ‘spare’ embryos to research, including stem-cell research, and makes a recommendation intended to enhance the quality of that process, including on occasion by guarding against the invalidity of such consent. This is important in its own right and also so as to maximise the reproductive treatment options of couples engaged in in vitro fertilisation (IVF) treatment and to avoid possible harms to them. In Part 1, with reference to qualitative data from three UK IVF clinics, we explore the often delicate and contingent nature of what comes to be, for legal purposes, a ‘spare’ embryo. The way in which an embryo becomes ‘spare’, with its implications for the process of consent to donation to research, is not addressed in the relevant reports relating to or codes of practice governing the donation of embryos to research, which assume an unproblematic notion of the ‘spare’ embryo. Significantly, our analysis demonstrates that there is an important and previously unrecognised first stage in the donation of a ‘spare’ embryo to research, namely: consent to an embryo being ‘spare’ and so, at the same time, to its disuse in treatment. This is not explicitly covered by the Human Fertilisation and Embryology (HFE) Act 1990, as amended by the HFE Act 2008. Having identified this important initial stage in the process of consent to the donation of a ‘spare’ embryo to research in conclusion to Part 1, in Part 2 we analyse the idea of consent to an embryo's disuse in treatment on the basis that it is ‘spare’ with reference to the legal elements of consent, namely information as to nature and purpose, capacity, and voluntariness. We argue that there are in fact three related consent processes in play, of which the principal one concerns consent to an embryo's disuse in treatment. If the quality of this first consent is compromised, in turn this will impact on the quality of the consent to the donation of that ‘spare’ embryo to research, followed by the quality of consent to future cycles of assisted reproduction treatment in the event that these are needed as a result of a donation decision. The analysis overall is of central relevance to the debate as to whether, and if so when, it should be permissible to request the donation of fresh embryos for research, as opposed to those that have been frozen and, for instance, have reached the end of their statutory storage term. This has a particular bearing on the donation of embryos to stem-cell research since there is a debate as to whether fresh embryos are most useful for this.This work is funded by the Wellcome Trust Biomedical Ethics Programme, Project Grant No 081414

Choosing embryos: Ethical complexity and relational autonomy in staff accounts of PGD

Copyright @ 2008 the authors. This article is available in accordance with the Creative Commons Deed, Attribution 2.5, see http://creativecommons.org/licenses/by-nc-nd/2.5/deed.en_CA.The technique of preimplantation genetic diagnosis (PGD) is commonly explained as a way of checking the genes of embryos produced by IVF for serious genetic diseases. However, complex accounts of this technique emerged during ethics discussion groups held for PGD staff. These form part of a study exploring the social processes, meanings and institutions that frame and produce ‘ethical problems’ for practitioners, scientists and others working in the specialty of PGD in the UK. Two ‘grey areas’ raised by staff are discussed in terms of how far staff are, or in the future may be, able to support autonomous choices of women/couples: accepting ‘carrier’ embryos within the goal of creating a ‘healthy’ child; and sex selection of embryos for social reasons. These grey areas challenged the staff’s resolve to offer individual informed choice, in the face of their awareness of possible collective social effects that might ensue from individual choices. We therefore argue that these new forms of choice pose a challenge to conventional models of individual autonomy used in UK genetic and reproductive counselling, and that ‘relational autonomy’ may be a more suitable ethical model to describe the ethical principles being drawn on by staff working in this area.The Wellcome Trust Biomedical Ethics Programme, who funded the project ‘Facilitating choice, framing choice: the experience of staff workingin pre-implantation genetic diagnosis’ (no: 074935)

Social welfare, genetic welfare? Boundary-work in the IVF/PGD clinic

Copyright @ 2006 Elsevier Ltd. All rights reserved.Through the lens of the ‘welfare of the child’ assessment, this paper explores how staff working in the area of in vitro fertilisation and preimplantation genetic diagnosis (IVF/PGD) balance reflexive relations of legitimacy and accountability between the public and private spheres, and between medicine, the citizen and the state. The wider research of which this analysis is a part uses multiple methods to study two National Health Service Assisted Conception Units in England. Research methods used included observation clinics and interviews with staff from a range of disciplines. We illustrate how the staff reveal tensions between their views that the welfare of the child assessment can be seen as intrusive and discriminatory, and on the other hand that medical intervention in reproduction should be socially and professionally accountable. These tensions can be understood sociologically in terms of a gradual movement from socially based solutions to fertility problems and disabilities, towards a biomedical, and arguably genetically oriented world view of such problems. Rather than being viewed as discrete, these two orientations should be seen as indicating an emergent direction of travel along a continuum, with elements of both being present in the accounts. We argue that consideration of the welfare of the child involves staff in ethical boundary-work across the two orientations and between the accountabilities and responsibilities of healthcare professionals, individuals and the state.The Wellcome Trust Biomedical Ethics Programme, who funded the project ‘Facilitating choice, framing choice: the experience of staff working in preimplantation genetic diagnosis’ (no. 074935)

Robust and stable transcriptional repression in Giardia using CRISPRi.

Giardia lamblia is a binucleate protistan parasite causing significant diarrheal disease worldwide. An inability to target Cas9 to both nuclei, combined with the lack of nonhomologous end joining and markers for positive selection, has stalled the adaptation of CRISPR/Cas9-mediated genetic tools for this widespread parasite. CRISPR interference (CRISPRi) is a modification of the CRISPR/Cas9 system that directs catalytically inactive Cas9 (dCas9) to target loci for stable transcriptional repression. Using a Giardia nuclear localization signal to target dCas9 to both nuclei, we developed efficient and stable CRISPRi-mediated transcriptional repression of exogenous and endogenous genes in Giardia. Specifically, CRISPRi knockdown of kinesin-2a and kinesin-13 causes severe flagellar length defects that mirror defects with morpholino knockdown. Knockdown of the ventral disk MBP protein also causes severe structural defects that are highly prevalent and persist in the population more than 5 d longer than defects associated with transient morpholino-based knockdown. By expressing two guide RNAs in tandem to simultaneously knock down kinesin-13 and MBP, we created a stable dual knockdown strain with both flagellar length and disk defects. The efficiency and simplicity of CRISPRi in polyploid Giardia allows rapid evaluation of knockdown phenotypes and highlights the utility of CRISPRi for emerging model systems

Profile: The Kilifi Health and Demographic Surveillance System (KHDSS).

The Kilifi Health and Demographic Surveillance System (KHDSS), located on the Indian Ocean coast of Kenya, was established in 2000 as a record of births, pregnancies, migration events and deaths and is maintained by 4-monthly household visits. The study area was selected to capture the majority of patients admitted to Kilifi District Hospital. The KHDSS has 260 000 residents and the hospital admits 4400 paediatric patients and 3400 adult patients per year. At the hospital, morbidity events are linked in real time by a computer search of the population register. Linked surveillance was extended to KHDSS vaccine clinics in 2008. KHDSS data have been used to define the incidence of hospital presentation with childhood infectious diseases (e.g. rotavirus diarrhoea, pneumococcal disease), to test the association between genetic risk factors (e.g. thalassaemia and sickle cell disease) and infectious diseases, to define the community prevalence of chronic diseases (e.g. epilepsy), to evaluate access to health care and to calculate the operational effectiveness of major public health interventions (e.g. conjugate Haemophilus influenzae type b vaccine). Rapport with residents is maintained through an active programme of community engagement. A system of collaborative engagement exists for sharing data on survival, morbidity, socio-economic status and vaccine coverage

Effectiveness of Deer Repellents in Connecticut

Browsing by overabundant herds of white-tailed deer (Odocoileus virginianus) can cause significant economic damage to agricultural crops and landscape plantings. In many instances, for both commercial growers and homeowners, commercially available repellents may be an appealing alternative to physical exclusion and lethal control of animals. We tested 10 different commercially-available repellents (Chew-Not®, Deer Off®, Deer-Away® Big Game Repellent, Plantskydd®, Bobbex®, Liquid Fence®, Deer Solution®, Hinder®, Repellex® systemic tablets, and coyote urine) on yews (Taxus cuspidata Densiformis) at 2 different locations in Connecticut. The study included both positive (fence) and negative (no treatment) controls. We planted yews in 2 blocks at each location in the spring of 2006; each block had 12 groups of 6 yews. We randomly assigned one of the 12 treatments to each group of yews within each block. We applied repellents based on manufacturers’ label recommendations for the 2006 and 2007 growing seasons and recorded application costs. We derived a protection index based on plant size and dry needle weights at the end of the 2007 growing season. In general, repellents that required more frequent application performed better. Bobbex® ranked highest, but was the most expensive repellent treatment. Hinder® performed nearly as well at a fraction of the cost. Yews protected by Repellex®, Deer Solution®, coyote urine, and Plantskydd® were the same size as unprotected controls at both sites and did not have significantly more needles. No repellents prevented 100% of browse damage. The choice of repellent usage is a trade-off among effectiveness, cost, ability to follow recommended reapplication interval, and plant to be protected

Investigating the Effect of Hand Position on Hand Force and Rotation Time When Performing a Freestyle Flip-Turn

Havriluk (2004) found that an increase in hand force will increase a swimmer’s velocity. While the effect of hand force on a swimmer’s velocity has been studied, the forces produced by the hands as well as the effect of forearm position on hand force production during a freestyle flip-turn has not been investigated. PURPOSE: To investigate the effect of forearm orientation on freestyle flip-turn performance. METHODS: A convenience sample of ten experienced swimmers (6 Female, 4 Male, 20 ± 1.15 years) was recruited to participate. Prior to data collection, participants completed an accommodation session to familiarize them with the techniques and protocol. Freestyle flip-turns were performed with both a swimming approach and a kicking only approach under two forearm position conditions: (1) pronated so that the palms faced upward and (2) supinated so that the palms faced downward. Rotation time in the turn, defined from initial downward movement of the feet prior to the turn to the instant the feet contact the wall, was measured using underwater video collected at 50 Hz during the trials using a swimming approach. Peak hand force was measured using a differential pressure transducer system (Aquanex, Inc.) during the trials using a kicking approach. A paired samples t-Test was used to compare rotation time between hand conditions. Hand force data were analyzed using a 2x2 (forearm position x hand) repeated measures ANOVA. RESULTS: The mean (sd) rotation times were 0.849 (0.075) s and 0.885 (0.094) s for the pronated condition and supinated condition, respectively. Rotation time was significantly faster under the pronated condition (p = 0.046). The mean (sd) peak force measurements were 6.76 (3.02) lbs, 5.67 (2.82) lbs, 4.82 (2.41) lbs, and 4.60 (2.51) for the left and right hand under the pronated and supinated condition, respectively. No significant main effect on hand force was found for forearm position (p = 0.146), and hand (p = 0.071). No significant interaction was found between forearm position and hand (p = 0.300). CONCLUSION: This study provides novel evidence that performing a freestyle flip-turn with the hands in the pronated position will lead to a faster rotation time and that there is no significant effect of hand position on hand force production during a freestyle flip-turn. As a majority of the participants felt more comfortable performing freestyle flip-turns with their hands in the pronated position, it is possible that additional accommodation to the novel technique is required to comfortably perform freestyle flip-turns with supinated forearms

Paper Session I-B - Modeling Methodology: The Use of Vehicle Control System Modeling for the International Space Station (ISS) Program

This paper presents a new control system software development approach entitled, RAPid Modeling and Analysis Philosophy (RAPMAP), and presents an example of the application of this approach on the International Space Station (ISS) program. RAPMAP embraces control system behavior modeling as an integral and ongoing part of the nominal development and integration process. Through a process called ‘Predictive-Verification,’ the RAPMAP approach ensures that development proceeds based on known and demonstrable performance and behavior throughout the development life cycle. The RAPMAP approach provides early identification of system integration issues and support for test and operational procedure development and training long before development products, which normally support these activities, are available. Section 1 of this paper introduces the concept of control system behavior modeling. Section 2 describes the RAPMAP approach and Section 3 provides a description of the the RAPMAP approach including a detailed description of its application on the ISS program. Section 4 summarizes the benefits of the RAPMAP approach

Size reconstructibility of graphs

The deck of a graph $G$ is given by the multiset of (unlabelled) subgraphs $\{G-v:v\in V(G)\}$. The subgraphs $G-v$ are referred to as the cards of $G$. Brown and Fenner recently showed that, for $n\geq29$, the number of edges of a graph $G$ can be computed from any deck missing 2 cards. We show that, for sufficiently large $n$, the number of edges can be computed from any deck missing at most $\frac1{20}\sqrt{n}$ cards.Comment: 15 page