XDJ1, a gene encoding a novel non-essential DnaJ homologue from Saccharomyces cerevisiae

The gene encoding a novel DnaJ-like protein, termed Xdj1, has been identified by amplification of Saccharomyces cerevisiae genomic DNA. An open reading frame of 1380 bp was detected. Disruption of XDJ1 did not yield any detectable new phenotype. A double-deletion strain containing a disruption of both XDJ1 and YDJ1, another gene coding for a DnaJ-like protein, was still viable. Under a variety of growth conditions, no XDJ1 transcripts could be detected by Northern blot analysis and no translation product was found by immunoblotting with antibody against Xdj1 produced in Escherichia coli. Thus, XDJ1 is either expressed only under very specific conditions or represents a silent gene

Completeness Conditions for Boundary Operators in 2D Conformal Field Theory

In non-diagonal conformal models, the boundary fields are not directly related to the bulk spectrum. We illustrate some of their features by completing previous work of Lewellen on sewing constraints for conformal theories in the presence of boundaries. As a result, we include additional open sectors in the descendants of $D_{odd}$ $SU(2)$ WZW models. A new phenomenon emerges, the appearance of multiplicities and fixed-point ambiguities in the boundary algebra not inherited from the closed sector. We conclude by deriving a set of polynomial equations, similar to those satisfied by the fusion-rule coefficients $N_{ij}^k$, for a new tensor $A_{a b}^i$ that determines the open spectrum.Comment: 13 pages, Latex, 3 figure

Экспериментальное исследование синхронного генератора периодических импульсов

Приводятся результаты экспериментального исследования влияния величины нагрузки синхронного генератора периодических импульсов с обычной геометрией зубцовой зоны на форму импульсов тока. Рассматривается возможность регулировки параметров импульсов

Untitled (Bowers, Hamilton, Schwarz, Steinfeld) Show Card

Show card for exhibition Untitled. September 7 - October 3, 2001.https://digitalcommons.udallas.edu/untitled/1001/thumbnail.jp

Open Descendants in Conformal Field Theory

Open descendants extend Conformal Field Theory to unoriented surfaces with boundaries. The construction rests on two types of generalizations of the fusion algebra. The first is needed even in the relatively simple case of diagonal models. It leads to a new tensor that satisfies the fusion algebra, but whose entries are signed integers. The second is needed when dealing with non-diagonal models, where Cardy's ansatz does not apply. It leads to a new tensor with positive integer entries, that satisfies a set of polynomial equations and encodes the classification of the allowed boundary operators.Comment: 19 pages, LATEX, 4 eps figures. Contribution to the Proceedings of the CERN Meeting on STU Dualities, Dec. 9

Towards autotrophic tissue engineering: Photosynthetic gene therapy for regeneration

Artículo científicoThe use of artificial tissues in regenerative medicine is limited due to hypoxia. As a strategy to overcome this drawback, we have shown that photosynthetic biomaterials can produce and provide oxygen independently of blood perfusion by generating chimeric animal-plant tissues during dermal regeneration. In this work, we demonstrate the safety and efficacy of photosynthetic biomaterials in vivo after engraftment in a fully immunocompetent mouse skin defect model. Further, we show that it is also possible to genetically engineer such photosynthetic scaffolds to deliver other key molecules in addition to oxygen. As a proof-of-concept, biomaterials were loaded with gene modified microalgae expressing the angiogenic recombinant protein VEGF. Survival of the algae, growth factor delivery and regenerative potential were evaluated in vitro and in vivo. This work proposes the use of photosynthetic gene therapy in regenerative medicine and provides scientific evidence for the use of engineered microalgae as an alternative to deliver recombinant molecules for gene therapy

Impact of plant protection strategy and soil tillage on the carbon footprint of wheat

Der Klimaschutz liegt aufgrund der zunehmenden negativen Auswirkungen des Klimawandels auf die pflanz­liche Produktion im Eigeninteresse der Landwirtschaft. Welche Auswirkungen unterschiedliche Pflanzenschutzstrategien, der Verzicht auf Fungizide sowie eine reduzierte Bodenbearbeitung auf die Klimawirkung der Weizenproduktion haben wurde aufbauend auf einem Dauerfeldversuch in Dahnsdorf in 2008–2019 untersucht. Die Bewertung wurden mittels partieller Lebenszyklus (LCA) durchgeführt. Im Durchschnitt über sämtliche Versuchsvarianten und Jahre lagen die Treibhausgasemissionen (THG-Emissionen) bei 3002 kg CO2eq ha‑1 und der CO2-Fußabdruck (CFP) bei 0,53 kg CO2eq kg‑1. Obwohl die Varianten mit wendender Bodenbearbeitung signifikant höhere THG-Emissionen je ha aufwiesen, waren Ihre CFPs nicht signifikant höher als die der nicht-wendenden Varianten. Die ertragssichernde Wirkung der Fungizide führte zu verringerten CFPs. Signifikante Jahresunterschiede zeigten sich bei allen drei untersuchten Parametern. Dies unterstreicht die Notwendigkeit einer Bewertung über längere Zeitreihen und den besonderen Wert von Langzeitversuchen.Climate protection is in the self-interest of agriculture due to the increasing negative effects of climate change on crop production. The effects of different crop protection strategies, the non-usage of fungicides, and reduced tillage were assessed regarding climate impact of wheat production based on a long-term field trial in Dahnsdorf in 2008–2019. The assessment was carried out using a partial life cycle assessment (LCA). On average across all treatments and years, greenhouse gas (GHG) emissions were 3002 kg CO2eq ha-1 and carbon footprint (CFP) was 0.53 kg CO2eq kg-1. Although the treatments with plow caused significantly higher GHG emissions per hectare, their CFPs were not significantly higher than those of the non-plowed treatments. The yield-securing effects of fungicides led to reduced CFPs. Significant differences between years were evident for all three parameters examined. This underlines the need for an evaluation over longer time series and the special value of long-term field trials

Роботизированные пожарные системы для защиты объектов с массовым пребыванием людей

В статье показано значение автоматизированных систем пожаротушения на базе пожарных роботов для защиты объектов с массовым пребыванием людей, рассмотрена их функциональная схема, проанализированы их достоинства, представлена нормативно-правовая база применения пожарных роботов

Rationale and design of the MULTISTARS AMI Trial: a randomized comparison of immediate versus staged complete revascularization in patients with ST-segment elevation myocardial infarction and multivessel disease

Background: About half of patients with acute ST-segment elevation myocardial infarction (STEMI) present with multivessel coronary artery disease (MVD). Recent evidence supports complete revascularization in these patients. However, optimal timing of non-culprit lesion revascularization in STEMI patients is unknown because dedicated randomized trials on this topic are lacking. Study design: The MULTISTARS AMI trial is a prospective, international, multicenter, randomized, two-arm, open-label study planning to enroll at least 840 patients. It is designed to investigate whether immediate complete revascularization is non-inferior to staged (within 19-45 days) complete revascularization in patients in stable hemodynamic conditions presenting with STEMI and MVD and undergoing primary percutaneous coronary intervention (PCI). After successful primary PCI of the culprit artery, patients are randomized in a 1:1 ratio to immediate or staged complete revascularization. The primary endpoint is a composite of all-cause death, non-fatal myocardial infarction, ischemia-driven revascularization, hospitalization for heart failure, and stroke at 1 year. Conclusions: The MULTISTARS AMI trial tests the hypothesis that immediate complete revascularization is non-inferior to staged complete revascularization in stable patients with STEMI and MVD

Comparing a Novel Neuroanimation Experience to Conventional Therapy for High-Dose Intensive Upper-Limb Training in Subacute Stroke: The SMARTS2 Randomized Trial

BACKGROUND Evidence from animal studies suggests that greater reductions in poststroke motor impairment can be attained with significantly higher doses and intensities of therapy focused on movement quality. These studies also indicate a dose-timing interaction, with more pronounced effects if high-intensity therapy is delivered in the acute/subacute, rather than chronic, poststroke period. OBJECTIVE To compare 2 approaches of delivering high-intensity, high-dose upper-limb therapy in patients with subacute stroke: a novel exploratory neuroanimation therapy (NAT) and modified conventional occupational therapy (COT). METHODS A total of 24 patients were randomized to NAT or COT and underwent 30 sessions of 60 minutes time-on-task in addition to standard care. The primary outcome was the Fugl-Meyer Upper Extremity motor score (FM-UE). Secondary outcomes included Action Research Arm Test (ARAT), grip strength, Stroke Impact Scale hand domain, and upper-limb kinematics. Outcomes were assessed at baseline, and days 3, 90, and 180 posttraining. Both groups were compared to a matched historical cohort (HC), which received only 30 minutes of upper-limb therapy per day. RESULTS There were no significant between-group differences in FM-UE change or any of the secondary outcomes at any timepoint. Both high-dose groups showed greater recovery on the ARAT (7.3 ± 2.9 points; P = .011) but not the FM-UE (1.4 ± 2.6 points; P = .564) when compared with the HC. CONCLUSIONS Neuroanimation may offer a new, enjoyable, efficient, and scalable way to deliver high-dose and intensive upper-limb therapy