813 research outputs found
High temperature silicon carbide impregnated insulating fabrics
High temperature insulating articles having improved performance characteristics are described. The articles comprise fabrics of closely woven refractory or heat resistant fibers having particles of silicon carbide dispersed at least partially through the fabric and bonded to the fibers with an emulsifiable polyethylene wax. Such articles exhibit significantly increased high temperature emittance characteristics and an improved retention of integrity and flexibility after prolonged exposure to high temperature
Superlattice with hot electron injection: an approach to a Bloch oscillator
A semiconductor superlattice with hot electron injection into the miniband is
considered. The injection changes the stationary distribution function and
results in a qualitative change of the frequency behaviour of the differential
conductivity. In the regime with Bloch oscillating electrons and injection into
the upper part of the miniband the region of negative differential conductivity
is shifted from low frequencies to higher frequencies. We find that the dc
differential conductivity can be made positive and thus the domain instability
can be suppressed. At the same time the high-frequency differential
conductivity is negative above the Bloch frequency. This opens a new way to
make a Bloch oscillator operating at THz frequencies.Comment: RevTeX, 8 pages, 2 figures, to be published in Phys. Rev. B, 15
Januar 200
Direct measurement of decoherence for entanglement between a photon and stored atomic excitation
Violations of a Bell inequality are reported for an experiment where one of
two entangled qubits is stored in a collective atomic memory for a user-defined
time delay. The atomic qubit is found to preserve the violation of a Bell
inequality for storage times up to 21 microseconds, 700 times longer than the
duration of the excitation pulse that creates the entanglement. To address the
question of the security of entanglement-based cryptography implemented with
this system, an investigation of the Bell violation as a function of the
cross-correlation between the generated nonclassical fields is reported, with
saturation of the violation close to the maximum value allowed by quantum
mechanics.Comment: 4 pages, 3 figures. Minor changes. Published versio
Carbohydrate Catabolism in Phaeobacter inhibens DSM 17395, a Member of the Marine Roseobacter Clade
Since genome analysis did not allow unambiguous reconstruction of transport, catabolism, and substrate-specific regulation for several important carbohydrates in Phaeobacter inhibens DSM 17395, proteomic and metabolomic analyses of N-acetylglucosamine-, mannitol-, sucrose-, glucose-, and xylose-grown cells were carried out to close this knowledge gap. These carbohydrates can pass through the outer membrane via porins identified in the outer membrane fraction. For transport across the cytoplasmic membrane, carbohydrate-specific ABC transport systems were identified. Their coding genes mostly colocalize with the respective "catabolic" and "regulatory" genes. The degradation of N-acetylglucosamine proceeds via N-acetylglucosamine-6-phosphate and glucosamine-6-phosphate directly to fructose-6-phosphate; two of the three enzymes involved were newly predicted and identified. Mannitol is catabolized via fructose, sucrose via fructose and glucose, glucose via glucose-6-phosphate, and xylose via xylulose-5-phosphate. Of the 30 proteins predicted to be involved in uptake, regulation, and degradation, 28 were identified by proteomics and 19 were assigned to their respective functions for the first time. The peripheral degradation pathways feed into the Entner-Doudoroff (ED) pathway, which is connected to the lower branch of the Embden-Meyerhof-Parnas (EMP) pathway. The enzyme constituents of these pathways displayed higher abundances in P. inhibens DSM 17395 cells grown with any of the five carbohydrates tested than in succinate-grown cells. Conversely, gluconeogenesis is turned on during succinate utilization. While tricarboxylic acid (TCA) cycle proteins remained mainly unchanged, the abundance profiles of their metabolites reflected the differing growth rates achieved with the different substrates tested. Homologs of the 74 genes involved in the reconstructed catabolic pathways and central metabolism are present in various Roseobacter clade members
MACiE: a database of enzyme reaction mechanisms.
SUMMARY: MACiE (mechanism, annotation and classification in enzymes) is a publicly available web-based database, held in CMLReact (an XML application), that aims to help our understanding of the evolution of enzyme catalytic mechanisms and also to create a classification system which reflects the actual chemical mechanism (catalytic steps) of an enzyme reaction, not only the overall reaction. AVAILABILITY: http://www-mitchell.ch.cam.ac.uk/macie/.EPSRC (G.L.H. and J.B.O.M.), the BBSRC (G.J.B. and J.M.T.—CASE studentship in association with Roche Products Ltd; N.M.O.B. and J.B.O.M.—grant BB/C51320X/1), the Chilean Government’s Ministerio de Planificacio´n y Cooperacio´n and
Cambridge Overseas Trust (D.E.A.) for funding and Unilever for supporting the Centre for Molecular Science Informatics.application note restricted to 2 printed pages web site: http://www-mitchell.ch.cam.ac.uk/macie
Generalized drift-diffusion model for miniband superlattices
A drift-diffusion model of miniband transport in strongly coupled
superlattices is derived from the single-miniband Boltzmann-Poisson transport
equation with a BGK (Bhatnagar-Gross-Krook) collision term. We use a consistent
Chapman-Enskog method to analyze the hyperbolic limit, at which collision and
electric field terms dominate the other terms in the Boltzmann equation. The
reduced equation is of the drift-diffusion type, but it includes additional
terms, and diffusion and drift do not obey the Einstein relation except in the
limit of high temperatures.Comment: 4 pages, 3 figures, double-column revtex. To appear as RC in PR
Selenium Status in Paediatric Patients with Neurodevelopmental Diseases
Neurodevelopmental diseases are often associated with other comorbidities, especially inflammatory processes. The disease may affect the trace element (TE) status, which in turn may affect disease severity and progression. Selenium (Se) is an essential TE required for the biosynthesis of selenoproteins including the transporter selenoprotein P (SELENOP) and extracellular glutathione peroxidase (GPX3). SELENOP deficiency in transgenic mice resulted in a Se status-dependent phenotype characterized by impaired growth and disturbed neuronal development, with epileptic seizures on a Se-deficient diet. Therefore, we hypothesized that Se and SELENOP deficiencies may be prevalent in paediatric patients with a neurodevelopmental disease. In an exploratory cross-sectional study, serum samples from children with neurodevelopmental diseases (n = 147) were analysed for total serum Se, copper (Cu), and zinc (Zn) concentrations as well as for the TE biomarkers SELENOP, ceruloplasmin (CP), and GPX3 activity. Children with epilepsy displayed elevated Cu and Zn concentrations but no dysregulation of serum Se status. Significantly reduced SELENOP concentrations were found in association with intellectual disability (mean +/- SD (standard deviation); 3.9 +/- 0.9 mg/L vs. 4.4 +/- 1.2 mg/L, p = 0.015). A particularly low GPX3 activity (mean +/- SD; 172.4 +/- 36.5 vs. 192.6 +/- 46.8 U/L, p = 0.012) was observed in phacomatoses. Autoantibodies to SELENOP, known to impair Se transport, were not detected in any of the children. In conclusion, there was no general association between Se deficiency and epilepsy in this observational analysis, which does not exclude its relevance to individual cases. Sufficiently high SELENOP concentrations seem to be of relevance to the support of normal mental development. Decreased GPX3 activity in phacomatoses may be relevant to the characteristic skin lesions and merits further analysis. Longitudinal studies are needed to determine whether the observed differences are relevant to disease progression and whether correcting a diagnosed TE deficiency may confer health benefits to affected children
Serum Selenium-Binding Protein 1 (SELENBP1) in Burn Injury: A Potential Biomarker of Disease Severity and Clinical Course
Oxidative stress, systemic inflammation, and metabolic derangements are hallmarks of burn pathophysiology. Severely burned patients are highly susceptible to infectious complications. Selenium-binding protein 1 (SELENBP1) modulates intracellular redox homeostasis, and elevated serum concentrations have been associated with adverse clinical outcomes in trauma patients. We hypothesized that serum SELENBP1 at hospital admission and during hospitalization may constitute a meaningful biomarker of disease severity and the clinical course in burn injury, with pulmonary infection as primary endpoint. To this end, we conducted a prospective cohort study that included 90 adult patients admitted to the Burn Center of the University Hospital Zurich, Switzerland. Patients were treated according to the local standard of care, with high-dose selenium supplementation during the first week. Serum SELENBP1 was determined at nine time-points up to six months postburn and the data were correlated to clinical parameters. SELENBP1 was initially elevated and rapidly declined within the first day. Baseline SELENBP1 levels correlated positively with the Abbreviated Burn Severity Index (ABSI) (R = 0.408; p < 0.0001). In multiple logistic regression, a higher ABSI was significantly associated with increased pulmonary infection risk (OR, 14.4; 95% CI, 3.2-88.8; p = 0.001). Similarly, baseline SELENBP1 levels constituted a novel but less accurate predictor of pulmonary infection risk (OR, 2.5; 95% CI, 0.7-8.9; p = 0.164). Further studies are needed to explore the additional value of serum SELENBP1 when stratifying patients with respect to the clinical course following major burns and, potentially, for monitoring therapeutic measures aimed at reducing tissue damage and oxidative stress
Regulation of Selenocysteine Content of Human Selenoprotein P by Dietary Selenium and Insertion of Cysteine in Place of Selenocysteine
Selenoproteins are a unique group of proteins that contain selenium in the
form of selenocysteine (Sec) co-translationally inserted in response to a UGA
codon with the help of cis- and trans-acting factors. Mammalian selenoproteins
contain single Sec residues, with the exception of selenoprotein P (SelP) that
has 7–15 Sec residues depending on species. Assessing an individual’s selenium
status is important under various pathological conditions, which requires a
reliable selenium biomarker. Due to a key role in organismal selenium
homeostasis, high Sec content, regulation by dietary selenium, and
availability of robust assays in human plasma, SelP has emerged as a major
biomarker of selenium status. Here, we found that Cys is present in various
Sec positions in human SelP. Treatment of cells expressing SelP with
thiophosphate, an analog of the selenium donor for Sec synthesis, led to a
nearly complete replacement of Sec with Cys, whereas supplementation of cells
with selenium supported Sec insertion. SelP isolated directly from human
plasma had up to 8% Cys inserted in place of Sec, depending on the Sec
position. These findings suggest that a change in selenium status may be
reflected in both SelP concentration and its Sec content, and that
availability of the SelP-derived selenium for selenoprotein synthesis may be
overestimated under conditions of low selenium status due to replacement of
Sec with Cys
Selenium status is positively associated with bone mineral density in healthy aging European men
Objective It is still a matter of debate if subtle changes in selenium (Se) status affect thyroid function tests (TFTs) and bone mineral density (BMD). This is particularly relevant for the elderly, whose nutritional status is more vulnerable. Design and Methods We investigated Se status in a cohort of 387 healthy elderly men (median age 77 yrs; inter quartile range 75-80 yrs) in relation to TFTs and BMD. Se status was determined by measuring both plasma selenoprotein P (SePP) and Se. Results The overall Se status in our population was low normal with only 0.5% (2/387) of subjects meeting the criteria for Se deficiency. SePP and Se levels were not associated with thyroid stimulating hormone (TSH), free thyroxine (FT4), thyroxine (T4), triiodothyronine (T3) or reverse triiodothyronine (rT3) levels. The T3/T4 and T3/rT3 ratios, reflecting peripheral metabolism of thyroid hormone, were not associated with Se status either. SePP and Se were positively associated with total BMD and femoral trochanter BMD. Se, but not SePP, was positively associated with femoral neck and ward's BMD. Multivariate linear analyses showed that these associations remain statistically significant in a model including TSH, FT4, body mass index, physical performance score, age, smoking, diabetes mellitus and number of medication use. Conclusion Our study demonstrates that Se status, within the normal European marginally supplied range, is positively associated with BMD in healthy aging men, independent of thyroid function. Thyroid function tests appear unaffected by Se status in this population
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