1,036 research outputs found
Potent neutralization by monoclonal human IgM against SARS-CoV-2 is impaired by class switch.
To investigate the class-dependent properties of anti-viral IgM antibodies, we use membrane antigen capture activated cell sorting to isolate spike-protein-specific B cells from donors recently infected with SARS-CoV-2, allowing production of recombinant antibodies. We isolate 20, spike-protein-specific antibodies of classes IgM, IgG, and IgA, none of which shows any antigen-independent binding to human cells. Two antibodies of class IgM mediate virus neutralization at picomolar concentrations, but this potency is lost following artificial switch to IgG. Although, as expected, the IgG versions of the antibodies appear to have lower avidity than their IgM parents, this is not sufficient to explain the loss of potency
Research opportunities for argumentation in social networks
Nowadays, many websites allow social networking between their users in an
explicit or implicit way. In this work, we show how argumentation schemes theory can
provide a valuable help to formalize and structure on-line discussions and user opinions in
decision support and business oriented websites that held social networks between their users.
Two real case studies are studied and analysed. Then, guidelines to enhance social decision
support and recommendations with argumentation are provided.This work summarises results of the authors joint research, funded by an STMS of the Agreement Technologies COST Action 0801, by the Spanish government grants [CONSOLIDER-INGENIO 2010 CSD2007-00022, and TIN2012-36586-C03-01] and by the GVA project [PROMETEO 2008/051].Heras Barberá, SM.; Atkinson, KM.; Botti Navarro, VJ.; Grasso, F.; Julian Inglada, VJ.; Mcburney, PJ. (2013). Research opportunities for argumentation in social networks. Artificial Intelligence Review. 39(1):39-62. doi:10.1007/s10462-012-9389-0S3962391Amgoud L (2009) Argumentation for decision making. Argumentation in artificial intelligence. Springer, BerlinAnderson P (2007) What is Web 2.0? Ideas, technologies and implications for education. JISC Iechnology and Standards Watch reportBentahar J, Meyer CJJ, Moulin B (2007) Securing agent-oriented systems: an argumentation and reputation-based approach. In: Proceedings of the 4th international conference on information technology: new generations (ITNG 2007), IEEE Computer Society, pp 507–515Buckingham Shum S (2008) Cohere: towards Web 2.0 argumentation. In: Proceedings of the 2nd international conference on computational models of argument, COMMA, pp 28–30Burke R (2002) Hybrid recommender systems: survey and experiments. User Model User-Adapt Interact 12:331–370Cartwright D, Atkinson K (2008) Political engagement through tools for argumentation. In: Proceedings of the second international conference on computational models of argument (COMMA 2008), pp 116–127Chesñevar C, McGinnis J, Modgil S, Rahwan I, Reed C, Simari G, South M, Vreeswijk G, Willmott S (2006) Towards an argument interchange format. Knowl Eng Rev 21(4):293–316Chesñevar CI, Maguitman AG, Gonzàlez MP (2009) Empowering recommendation technologies through argumentation. Argumentation in artificial intelligence. Springer, Berlin, pp 403–422García AJ, Dix J, Simari GR (2009) Argument-based logic programming. Argumentation in artificial intelligence. Springer, BerlinGolbeck J (2006) Generating predictive movie recommendations from trust in social networks. In: Proceedings of the fourth international conference on trust management, LNCS, vol 3986, 93–104Gordon T, Prakken H, Walton D (2007) The Carneades model of argument and burden of proof. Artif Intell 171(10–15):875–896Guha R, Kumar R, Raghavan P, Tomkins A (2004) Propagating trust and distrust. In: Proceedings of the 13th international conference on, World Wide Web, pp 403–412Heras S, Navarro M, Botti V, Julián V (2009) Applying dialogue games to manage recommendation in social networks. In: Proceedings of the 6th international workshop on argumentation in multi-agent aystems, ArgMASHeras S, Atkinson K, Botti V, Grasso F, Julián V, McBurney P (2010a) How argumentation can enhance dialogues in social networks. In: Proceedings of the 3rd international conference on computational models of argument, COMMA, vol 216, pp 267–274Heras S, Atkinson K, Botti V, Grasso F, Julián V, McBurney P (2010b) Applying argumentation to enhance dialogues in social networks. In: ECAI 2010 workshop on computational models of natural argument, CMNA, pp 10–17Karacapilidis N, Tzagarakis M (2007) Web-based collaboration and decision making support: a multi-disciplinary approach. Web-Based Learn Teach Technol 2(4):12–23Kim D, Benbasat I (2003) Trust-related arguments in internet stores: a framework for evaluation. J Electron Commer Res 4(2):49–64Kim D, Benbasat I (2006) The effects of trust-assuring arguments on consumer trust in internet stores: application of Toulmin’s model of argumentation. Inf Syst Rese 17(3):286–300Laera L, Tamma V, Euzenat J, Bench-Capon T, Payne T (2006) Reaching agreement over ontology alignments. In: Proceedings of the 5th international semantic web conference (ISWC 2006)Lange C, Bojãrs U, Groza T, Breslin J, Handschuh S (2008) Expressing argumentative discussions in social media sites. In: Social data on the web (SDoW2008) workshop at the 7th international semantic web conferenceLinden G, Smith B, York J (2003) Amazon.com recommendations: item-to-item collaborative filtering. IEEE Internet Comput 7(1):76–80Linden G, Hong J, Stonebraker M, Guzdial M (2009) Recommendation algorithms, online privacy and more. Commun ACM, 52(5)Mika P (2007) Ontologies are us: a unified model of social networks and semantics. J Web Semant 5(1):5–15Montaner M, López B, de la Rosa JL (2002) Opinion-based filtering through trust. In: Cooperative information agents VI, LNCS, vol 2446, pp 127–144Ontañón S, Plaza E (2008) Argumentation-based information exchange in prediction markets. In: Proceedings of the 5th international workshop on argumentation in multi-agent systems, ArgMASPazzani MJ, Billsus D (2007) Content-based recommendation systems. In: The adaptive web, LNCS, vol 4321, pp 325–341Rahwan I, Zablith F, Reed C (2007) Laying the foundations for a world wide argument web. Artif Intell 171(10–15):897–921Rahwan I, Banihashemi B (2008) Arguments in OWL: a progress report. In: Proceedings of the 2nd international conference on computational models of argument (COMMA), pp 297–310Reed C, Walton D (2007) Argumentation schemes in dialogue. In: Dissensus and the search for common ground, OSSA-07, volume CD-ROM, pp 1–11Sabater J, Sierra C (2002) Reputation and social network analysis in multi-agent systems. In: Proceedings of the 1st international joint conference on autonomous agents and multiagent systems, vol 1, pp 475–482Schafer JB, Konstan JA, Riedl J (2001) E-commerce recommendation applications. Data Min Knowl Discov 5:115–153Schafer JB, Frankowski D, Herlocker J, Sen S (2007) Collaborative filtering recommender systems. In: The adaptive web, LNCS, vol 4321, pp 291–324Schneider J, Groza T, Passant A (2012) A review of argumentation for the aocial semantic web. Semantic web-interoperability, usability, applicability. IOS Press, Washington, DCTempich C, Pinto HS, Sure Y, Staab S (2005) An argumentation ontology for distributed, loosely-controlled and evolvInG Engineering processes of oNTologies (DILIGENT). In: Proceedings of the 2nd European semantic web conference, ESWC, pp 241–256Toulmin SE (1958) The uses of argument. Cambridge University Press, Cambridge, UKTrojahn C, Quaresma P, Vieira R, Isaac A (2009) Comparing argumentation frameworks for composite ontology matching. in: Proceedings of the 6th international workshop on argumentation in multi-agent systems, ArgMASTruthMapping. http://truthmapping.com/Walter FE, Battiston S, Schweitzer F (2007) A model of a trust-based recommendation system on a social network. J Auton Agents Multi-Agent Syst 16(1):57–74Walton D, Krabbe E (1995) Commitment in dialogue: basic concepts of interpersonal reasoning. State University of New York Press, New York, NYWalton D, Reed C, Macagno F (2008) Argumentation schemes. Cambridge University Press, CambridgeWells S, Gourlay C, Reed C (2009) Argument blogging. Computational models of natural argument, CMNAWyner A, Schneider J (2012) Arguing from a point of view. In: Proceedings of the first international conference on agreement technologie
Cell-binding IgM in CSF is distinctive of multiple sclerosis and targets the iron transporter SCARA5
Intrathecal IgM production in multiple sclerosis (MS) is associated with a worse disease course. To investigate pathogenic relevance of autoreactive IgM in MS, CSF from two independent cohorts, including MS patients and controls, were screened for antibody binding to induced pluripotent stem cell-derived neurons and astrocytes, and a panel of CNS- related cell lines. IgM binding to a primitive neuro-ectodermal tumour cell line discriminated 10% of MS donors from controls. Transcriptomes of single IgM producing CSF B cells from patients with cell-binding IgM were sequenced and used to produce recombinant monoclonal antibodies for characterisation and antigen identification. We produced 5 cell-binding recombinant IgM antibodies, of which one, cloned from an HLA-DR + plasma-like B cell, mediated antigen-dependent complement activation. Immunoprecipitation and mass spectrometry, and biochemical and transcriptome analysis of the target cells identified the iron transport scavenger protein SCARA5 as the antigen target of this antibody. Intrathecal injection of a SCARA5 antibody led to an increased T cell infiltration in an EAE model. CSF IgM might contribute to CNS inflammation in MS by binding to cell surface antigens like SCARA5 and activating complement, or by facilitating immune cell migration into the brain
Forward pi^0 Production and Associated Transverse Energy Flow in Deep-Inelastic Scattering at HERA
Deep-inelastic positron-proton interactions at low values of Bjorken-x down
to x \approx 4.10^-5 which give rise to high transverse momentum pi^0 mesons
are studied with the H1 experiment at HERA. The inclusive cross section for
pi^0 mesons produced at small angles with respect to the proton remnant (the
forward region) is presented as a function of the transverse momentum and
energy of the pi^0 and of the four-momentum transfer Q^2 and Bjorken-x.
Measurements are also presented of the transverse energy flow in events
containing a forward pi^0 meson. Hadronic final state calculations based on QCD
models implementing different parton evolution schemes are confronted with the
data.Comment: 27 pages, 8 figures and 3 table
Influence of sagittal balance and physical ability associated with exercise on quality of life in middle-aged and elderly people
Searching for Explanations for Cryptogenic Stroke in the Young: Revealing the Triggers, Causes, and Outcome (SECRETO): Rationale and design
Background: Worldwide, about 1.3 million annual ischaemic strokes (IS) occur in adults aged <50 years. Of these
early-onset strokes, up to 50% can be regarded as cryptogenic or associated with conditions with poorly documented
causality like patent foramen ovale and coagulopathies.
Key hypotheses/aims: (1) Investigate transient triggers and clinical/sub-clinical chronic risk factors associated with
cryptogenic IS in the young; (2) use cardiac imaging methods exceeding state-of-the-art to reveal novel sources for
embolism; (3) search for covert thrombosis and haemostasis abnormalities; (4) discover new disease pathways using
next-generation sequencing and RNA gene expression studies; (5) determine patient prognosis by use of phenotypic and
genetic data; and (6) adapt systems medicine approach to investigate complex risk-factor interactions.
Design: Searching for Explanations for Cryptogenic Stroke in the Young: Revealing the Etiology, Triggers, and Outcome
(SECRETO; NCT01934725) is a prospective multi-centre case–control study enrolling patients aged 18–49 years hospitalised
due to first-ever imaging-proven IS of undetermined etiology. Patients are examined according to a standardised
protocol and followed up for 10 years. Patients are 1:1 age- and sex-matched to stroke-free controls. Key study elements
include centralised reading of echocardiography, electrocardiography, and neurovascular imaging, as well as blood samples
for genetic, gene-expression, thrombosis and haemostasis and biomarker analysis. We aim to have 600 patient–
control pairs enrolled by the end of 2018.
Summary: SECRETO is aiming to establish novel mechanisms and prognosis of cryptogenic IS in the young and will
provide new directions for therapy development for these patients. First results are anticipated in 2019
Bone mineral density in partially recovered early onset anorexic patients - a follow-up investigation
<p>Abstract</p> <p>Background and aims</p> <p>There still is a lack of prospective studies on bone mineral development in patients with a history of early onset Anorexia nervosa (AN). Therefore we assessed associations between bone mass accrual and clinical outcomes in a former clinical sample. In addition to an expected influence of regular physical activity and hormone replacement therapy, we explored correlations with nutritionally dependent hormones.</p> <p>Methods</p> <p>3-9 years (mean 5.2 ± 1.7) after hospital discharge, we re-investigated 52 female subjects with a history of early onset AN. By means of a standardized approach, we evaluated the general outcome of AN. Moreover, bone mineral content (BMC) and bone mineral density (BMD) as well as lean and fat mass were measured by dual-energy x-ray absorptiometry (DXA). In a substudy, we measured the serum concentrations of leptin and insulin-like growth factor-I (IGF-I).</p> <p>Results</p> <p>The general outcome of anorexia nervosa was good in 50% of the subjects (BMI ≥ 17.5 kg/m<sup>2</sup>, resumption of menses). Clinical improvement was correlated with BMC and BMD accrual (χ<sup>2 </sup>= 5.62/χ<sup>2 </sup>= 6.65, p = 0.06 / p = 0.036). The duration of amenorrhea had a negative correlation with BMD (r = -.362; p < 0.01), but not with BMC. Regular physical activity tended to show a positive effect on bone recovery, but the effect of hormone replacement therapy was not significant. Using age-related standards, the post-discharge sample for the substudy presented IGF-I levels below the 5<sup>th </sup>percentile. IGF-I serum concentrations corresponded to the general outcome of AN. By contrast, leptin serum concentrations showed great variability. They correlated with BMC and current body composition parameters.</p> <p>Conclusions</p> <p>Our results from the main study indicate a certain adaptability of bone mineral accrual which is dependent on a speedy and ongoing recovery. While leptin levels in the substudy tended to respond immediately to current nutritional status, IGF-I serum concentrations corresponded to the individual's age and general outcome of AN.</p
Prevalence of Drug-Resistant HIV-1 Variants in Untreated Individuals in Europe: Implications for Clinical Management
BackgroundInfection with drug-resistant human immunodeficiency virus type 1 (HIV-1) can impair the response to combination therapy. Widespread transmission of drug-resistant variants has the disturbing potential of limiting future therapy options and affecting the efficacy of postexposure prophylaxis penta increase-spacing 1>MethodsWe determined the baseline rate of drug resistance in 2208 therapy-naive patients recently and chronically infected with HIV-1 from 19 European countries during 1996-2002 ResultsIn Europe, 1 of 10 antiretroviral-naive patients carried viruses with ⩾1 drug-resistance mutation. Recently infected patients harbored resistant variants more often than did chronically infected patients (13.5% vs. 8.7%; P=.006). Non-B viruses (30%) less frequently carried resistance mutations than did subtype B viruses (4.8% vs. 12.9%; P<.01). Baseline resistance increased over time in newly diagnosed cases of non-B infection: from 2.0% (1/49) in 1996-1998 to 8.2% (16/194) in 2000-2001 ConclusionsDrug-resistant variants are frequently present in both recently and chronically infected therapy-naive patients. Drug-resistant variants are most commonly seen in patients infected with subtype B virus, probably because of longer exposure of these viruses to drugs. However, an increase in baseline resistance in non-B viruses is observed. These data argue for testing all drug-naive patients and are of relevance when guidelines for management of postexposure prophylaxis and first-line therapy are update
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An overview of the first decade of PollyNET: An emerging network of automated Raman-polarization lidars for continuous aerosol profiling
A global vertically resolved aerosol data set covering more than 10 years of observations at more than 20 measurement sites distributed from 63° N to 52° S and 72° W to 124° E has been achieved within the Raman and polarization lidar network PollyNET. This network consists of portable, remote-controlled multiwavelength-polarization-Raman lidars (Polly) for automated and continuous 24/7 observations of clouds and aerosols. PollyNET is an independent, voluntary, and scientific network. All Polly lidars feature a standardized instrument design with different capabilities ranging from single wavelength to multiwavelength systems, and now apply unified calibration, quality control, and data analysis. The observations are processed in near-real time without manual intervention, and are presented online at http://polly.tropos.de/. The paper gives an overview of the observations on four continents and two research vessels obtained with eight Polly systems. The specific aerosol types at these locations (mineral dust, smoke, dust-smoke and other dusty mixtures, urban haze, and volcanic ash) are identified by their Ångström exponent, lidar ratio, and depolarization ratio. The vertical aerosol distribution at the PollyNET locations is discussed on the basis of more than 55 000 automatically retrieved 30 min particle backscatter coefficient profiles at 532 nm as this operating wavelength is available for all Polly lidar systems. A seasonal analysis of measurements at selected sites revealed typical and extraordinary aerosol conditions as well as seasonal differences. These studies show the potential of PollyNET to support the establishment of a global aerosol climatology that covers the entire troposphere
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