The Stratigraphy And Depositional History Of The Deadwood Formation, With A Focus On Early Paleozoic Subsidence In The Williston Basin

The Deadwood Formation is an assemblage of siliciclastic, carbonate, and evaporite sedimentary rocks in North Dakota, South Dakota, Montana, Wyoming, Manitoba, and Saskatchewan. The majority of the lateral extent of the Deadwood Formation is in the subsurface of the Williston Basin, where it is the basal lithostratigraphic unit. Deposition began roughly 501 million years ago, as the Sauk sequence reached the exposed Precambrian igneous and metamorphic rock of the North American Craton. Six identifiable and widespread gamma ray markers occur in the well logs, dividing the formation into six informal units, label members A through F in ascending order. The initial deposits on the craton were conglomerates and sandstones of the Cambrian Member A. These sediments were overlain by glauconite rich, siltstones and fine-grained sandstones of the Cambrian and Ordovician Member B. After the deposition of Member B, three regressive-transgressive sequences took place, depositing a succession of sandstones, limestones, dolomudstones, siliciclastic mudstones, and calcareous siltstones. These deposits represent the Ordovician members, C, D, E and F. Using the thickness, depositional environments, age of each member, and other well information, tectonic subsidence values were determined using backstripping analysis. This analysis was completed by inputting all of the information into Novva®, a 1D geological modeling software released by Sirius Exploration Geochemistry Inc. Data collected from well logs and core, other data researched by the author, and information from previous works was combined with information and calculations supplied by Novva®. The results produce an accurate computation of the depositional history for the seven wells that penetrated all six members of the Deadwood Formation and the Precambrian basement. Prior to and at the start of Deadwood deposition the Williston Basin did not exist. Evidence from isopach maps created for each member of the Deadwood Formation and the results from Novva® concluded that subsidence in the area, now known as the Williston Basin, did not begin until Member C was being deposited. This places the initiation of the Williston Basin to be roughly 485 to 482 million years ago

The Law Review Selection Process: An Analysis of Its Disparate Impact on Minority Students

Helen I. Kelly Awar

Women as a political force : did the vote make a difference?

Includes bibliographical references.Whether from traditional history books or women’s studies texts, the pronouncement on women and the vote seems to be the same. The preceding quotes are the conventional assumption of women as a political force. After the vote, we are told, women went back home and nothing changed. With no driving issue, women became apathetical. This is not only a wrong historical fact, but a dangerous one for women. If accepted, it can be used as a weapon against women. Ie: Women aren’t really political. Women did not exercise rights anyway. Suffragists were a small, unimportant group. Most women don’t care about the public sphere. The same thing will happen with ERA, so why bother? I suggest that we will prove the historians’ verdict false by an examination of women’s activities both before and after the 19th Amendment. This examination will focus on three areas in which women were an active force: labor, social reform, and moral reform

Antibiotic-dependent expression of early transcription factor subunits leads to stringent control of vaccinia virus replication

AbstractThe use of vaccinia virus (VACV) as the vaccine against variola virus resulted in the eradication of smallpox. VACV has since been used in the development of recombinant vaccine and therapeutic vectors, but complications associated with uncontrolled viral replication have constrained its use as a live viral vector. We propose to improve the safety of VACV as a live-replicating vector by using elements of the tet operon to control the transcription of genes that are essential for viral growth. Poxviruses encode all enzymes and factors necessary for their replication within the host cell cytoplasm. One essential VACV factor is the vaccinia early transcription factor (VETF) packaged into the viral core. This heterodimeric protein is required for expression of early VACV genes. VETF is composed of a large subunit encoded by the A7L gene and a small subunit encoded by the D6R gene. Two recombinant VACVs were generated in which either the A7L or D6R gene was placed under the control of tet operon elements to allow their transcription, and therefore viral replication, to be dependent on tetracycline antibiotics such as doxycycline. In the absence of inducers, no plaques were produced but abortively infected cells could be identified by expression of a reporter gene. In the presence of doxycycline, both recombinant viruses replicated indistinguishably from the wild-type strain. This stringent control of VACV replication can be used for the development of safer, next-generation VACV vaccines and therapeutic vectors. Such replication-inducible VACVs would only replicate when administered with tetracycline antibiotics, and if adverse events were to occur, treatment would be as simple as antibiotic cessation

Biomarkers to identify and isolate senescent cells.

Aging is the main risk factor for many degenerative diseases and declining health. Senescent cells are part of the underlying mechanism for time-dependent tissue dysfunction. These cells can negatively affect neighbouring cells through an altered secretory phenotype: the senescence-associated secretory phenotype (SASP). The SASP induces senescence in healthy cells, promotes tumour formation and progression, and contributes to other age-related diseases such as atherosclerosis, immune-senescence and neurodegeneration. Removal of senescent cells was recently demonstrated to delay age-related degeneration and extend lifespan. To better understand cell aging and to reap the benefits of senescent cell removal, it is necessary to have a reliable biomarker to identify these cells. Following an introduction to cellular senescence, we discuss several classes of biomarkers in the context of their utility in identifying and/or removing senescent cells from tissues. Although senescence can be induced by a variety of stimuli, senescent cells share some characteristics that enable their identification both in vitro and in vivo. Nevertheless, it may prove difficult to identify a single biomarker capable of distinguishing senescence in all cell types. Therefore, this will not be a comprehensive review of all senescence biomarkers but rather an outlook on technologies and markers that are most suitable to identify and isolate senescent cells

A Comparison of the Volatile Components of Cold Pressed Hamlin and Valencia ( Citrus sinensis

Volatiles from huanglongbing (HLB) symptomatic and asymptomatic cold pressed orange oils from Florida Hamlin and Valencia fruit were assessed. Qualitative gas-liquid chromatography studies showed the presence of several compounds (β-longifolene, perillene, and 4-decenal) which are not commonly identified in Citrus sinensis (L.) Osbeck oils. Oils derived from huanglongbing symptomatic fruit had lower concentrations of linalool, decanal, citronellol, neral, geranial, carvone, dodecanal, and 2-decenal and higher concentrations of citronellal compared to asymptomatic fruit. A comparison to historic literature of orange oil investigations before HLB was of issue in Florida orange crops showed lower levels of linalool, decanal, neral, and geranial in Hamlin peel oil samples, as well as higher levels of dodecanal. Valencia peel oil samples showed lower concentrations of linalool and increased concentration of citronellol and dodecanal. As a result of huanglongbing (HLB) phenomena, the concentrations of several important volatiles found in Hamlin and Valencia peel oil profiles have changed compared to historic values. Differences in volatile concentrations of symptomatic and asymptomatic HLB affected peel oil compounds in orange fruit are identified

Heat stress during seed development leads to impaired physiological function and plasticity in seed oil accumulation in Camelina sativa

Camelina sativa, a member of the Brassicaceae, is a low-cost, renewable oilseed crop that produces seeds up to 40% oil by weight with high potential for use in food, feed, and biofuel applications. Camelina seeds contain high levels of the fatty acids α-linolenic acid (C18:3), linoleic acid (C18:2), oleic acid (C18:1), and gondoic acid (C20:1), which have high nutritional and industrial value. The impact of climate change, especially increased frequency and amplitude of heat waves, poses a serious threat to crop productivity. In this study, we evaluated the effect of elevated temperatures post-anthesis on the developing seeds of C. sativa and performed physiological, morphological, and chemical characterizations at 7, 14, 21, and 28 days post-anthesis (DPA), as well as at maturity. While the seed oil accumulation peaked at 21 DPA under control conditions, reaching 406mg/g dry weight, under heat stress it was only 186mg/g. Physiologically, transpiration rate (E) and internal CO2 concentration (Ci) increased between 2 to 9 days post-stress imposition and overall net photosynthesis was impaired. Seed yield, seed weight, and oil content reduced by 84.5%, 38.5% and 54.1% respectively. We demonstrate that post-anthesis heat stress causes severe yield losses and developmental plasticity in fatty acid accumulation in oilseeds

Antimicrobial potential of LEGUMES extracts against foodborne pathogens: A review

[EN] Background: Alternative protein sources are being investigated in response to increasing consumer demand for innovative and healthy food products of vegetable origin to replace non-sustainable animal exploitation. The Leguminosae family includes a wide variety of plants and nutritious seeds, very rich in protein with a high biological value, carbohydrates, vitamins and minerals. Not only the seeds but also the aerial parts, pods, hulls and roots have proved to be natural sources of antioxidants, and anti-inflammatory and antimicrobial compounds. Scope and approach: The present article overviews the antimicrobial potential of the most popular legumes worldwide against foodborne pathogens. Key findings and conclusions: According to the literature reviewed, soybean and chickpea are the two consumed legumes with the highest antimicrobial activity. Long-chain soy peptides (IKAFKEATKVDKVVVLWTA) have a high antimicrobial potential against both Gram-positive and Gram-negative bacteria at a concentration level of 37.2 ¿M. Also, a wide spectrum of proteins and peptides in raw chickpeas and processed extracts have exerted antimicrobial activity against foodborne pathogens when applied in the range 8¿64 ¿g/ml. These results open a new research line with good prospects regarding the development of a new generation of natural preservative ingredients and extracts to be included in novel formulated products. However, critical aspects, such as (i) the stability of antimicrobial activity during the shelf-life of newly formulated food products, and (ii) the microbial inactivation kinetics generated in novel matrices, should be covered prior to exploitation of legumes as sources of novel technological ingredients with antimicrobial potential.The present research work has been supported by funds provided by the Spanish Ministry of Economy and Competitiveness (MINECO) as the HELICOFOOD project, with reference AGL2014-53875-R. The post-doctoral contract of M.C. Pina-Pérez as Juan de la Cierva-Incorporación granted by the MINECO is also acknowledged.Pina Pérez, MC.; Ferrús Pérez, MA. (2018). Antimicrobial potential of LEGUMES extracts against foodborne pathogens: A review. Trends in Food Science & Technology. 72:114-124. doi:10.1016/j.tifs.2017.12.007S1141247