Effectiveness of Lactobacillus Plantarum Mut 7 Agents Fermentation to Reduce Trembesi (Albizia Saman) Saponins Content

This objectives research to assess the ability of Lactobacillus plantarum Mut 7 in reducing the saponincontent while increasing the nutrient content in the leaves, young fruit and trembesi pods (Albiziasaman). The study was conducted using a completely randomized design. The addition of Lactobacillusplantarum Mut 7 on the fermentation of 3 parts, namely plant leaves, young fruit and pods. Each treatmentwas repeated as many as four replications to obtain 12 units of the experiment. This study concludedthat the fermentation using Lactobacillus plantarum Mut 7 reduced saponin content and increase nutrientcontent, especially the content of crude protein

Trembesi Digestibility Fermented By Lactobacillus Plantarum Mut 7

            The specific purpose of this third year research is to study the level of digestibility in vivo BTTF and BTF and to study the effect of the use of tamarind leaves by the method of increasing the quality of nutrient content and decreasing saponin in PO cattle feed (ongole breeds). The specific target of the third year is BTTF and BTF in vivo digestibility. This study was designed with a Completely Randomized Directional Design with two treatments, namely in vivo digestibility BTTF and BTF. From this study it can be concluded that the level of digestibility in vivo from BTF was 53.33% and was significantly different from BTTF. Keywords: in vivo digestibility, trembes

Tear protein interaction with hydrogel contact lenses

The design and synthesis of biomaterials covers a growing number of biomedical applications. The use of biomaterials in biological environment is associated with a number of problems, the most important of which is biocompatabUity. If the implanted biomaterial is not compatible with the environment, it will be rejected by the biological site. This may be manifested in many ways depending on the environment in which it is used. Adsorption of proteins takes place almost instantaneously when a biomaterial comes into contact with most biological fluids. The eye is a unique body site for the study of protein interactions with biomaterials, because of its ease of access and deceptive complexity of the tears. The use of contact lenses for either vision correction and cosmetic reasons or as a route for the controlled drug delivery, has significantly increased in recent years. It is relatively easy to introduce a contact lens Into the tear fluid and remove after a few minutes without surgery or trauma to the patient. A range of analytical techniques were used and developed to measure the proteins absorbed to some existing commercial contact lens materials and also to novel hydrogels synthesised within the research group. Analysis of the identity and quantity of proteins absorbed to biomaterials revealed the importance of many factors on the absorption process. The effect of biomaterial structure, protein nature in terms of size. shape and charge and pH of the environment on the absorption process were examined in order to determine the relative up-take of tear proteins. This study showed that both lysozyme and lactoferrin penetrate the lens matrix of ionic materials. Measurement of the mobility and activity of the protein deposited into the surface and within the matrix of ionic lens materials demonstrated that the mobility is pH dependent and, within the experimental errors, the biological activity of lysozyme remained unchanged after adsorption and desorption. The study on the effect of different monomers copolymerised with hydroxyethyl methacrylate (HEMA) on the protein up-take showed that monomers producing a positive charge on the copolymer can reduce the spoilation with lysozyme. The studies were extended to real cases in order to compare the patient dependent factors. The in-vivo studies showed that the spoilation is patient dependent as well as other factors. Studies on the extrinsic factors such as dye used in colour lenses showed that the addition of colourant affects protein absorption and, in one case, its effect is beneficial to the wearer as it reduces the quantity of the protein absorbed

Sharp Response Microstrip LPF using Folded Stepped Impedance Open Stubs

A novel microstrip lowpass filter with high selectivity and wide stopband is proposed that comprises two lateral folded open stubs and a central mirrored semi-circle ended suppressing cell. The proposed filter has cut-off frequency of 2.28 GHz and is very compact. The stopband width with attenuation level more than -20 dB is equal to 5.47 fc and the transition band is only 0.14 GHz. This filter is designed, fabricated and measured and the simulated and measured results are in good agreemen

Protein Digestibility of Trembesi Leaf Fermented with Lactobacillus plantarum AK-3

This study aims to assess the digestibility of trembesi leaf protein fermented with Lactobacillus plantarum AK-3. Trembesi leaves are fermented by adding Lactobacillus plantarum AK 3 which is isolated from cow's milk waste. Then the protein digestibility was analyzed for ruminants. This research is descriptive so the data obtained is descriptive data about the level of digestibility of crude protein from fermented leaves. The conclusion obtained is the level of crude protein digestibility of the leaves of fermented trembesi Lactobacillus plantarum AK-3 by 71%.Key word:  fermentation, trembesi leaf,  protein digestibility, Lactobacillus plantarum AK-

NUTRIENT CONTENT OF COCOA POD (Theobroma cocoa) FERMENTATION WITH Aspergilllus niger ADDITION

The study was conducted to determine the increase in nutrient content and decrease in the lignin content of cocoa pod fermented with Aspergillus niger. The research method used three treatments and four replications. T0 = cocoa pod fermented without the addition of Aspergillus niger, T1 = cocoa pod fermented with the addition of 1% Aspergillus niger, and T2 = cocoa pod fermented with the addition of 2% Aspergillus niger. Fresh cocoa pod was chopped in 1-2 cm size. Cocoa pod chopped and dried, some added with Aspergillus niger. The mixture is put into an aerobic container for 7 days. The variables observed included dry matter, crude protein, crude fiber, and crude fat. This study was designed using a complete randomized design research design with one-way ANOVA analysis. Significant variables were followed by Duncan's multiple range test (Duncan multiple range test / DMRT). The results of the study showed significantly different results in all treatments. It was concluded that the fermentation with addition Aspergillus niger until 2% decrease crude protein, crude fiber, and crude fat on cocoa pod fermentation

Lama Penyimpanan Sosis Ayam Menggunakan Edible Film dari Gelatin Limbah Tulang Ayam Terhadap Kualitas Kimia

ABSTRAKSosis adalah produk yang dibuat dengan campuran berbagai bahan dengan bahan baku utama adalah daging. Penelitian ini bertujuan untuk mengetahui pengaruh lama simpan sosis ayam menggunakan edible film dari gelatin limbah tulang ayam terhadap kualitas  kimia. Metode penelitian ini menggunakan rancangan acak lengkap (RAL) dengan satu faktor perlakuan, yaitu lama penyimpanan. Perlakuan penelitian menggunakan coating edible film dari gelatin limbah tulang ayam terhadap lama penyimpanan sosis ayam. Data yang diperoleh dianalisis secara statistik menggunakan ragam. Parameter pengamatan penelitian meliputi uji nilai Ph, kadar air, kadar protein dan kadar lemak. Hasil penelotian menunjukkan penggunaan edible film dari gelatin limbah tulang ayam terhadap lama penyimpanan sosis ayam selama 9 hari berpengaruh nyata terhadap nilai ph, kadar air, kadar protein dan kadar lemak. Kata kunci: edible film, kualitas kimia, limbah tulang ayam, sosis ayam Long Storage Time for Chicken Sausage using Edible Film from Gelatin Waste Chicken Bone to Chemical Quality                                                           ABSTRACT      Sausage is a product made with a mixture of various ingredients with the main raw material being meat. This study aims to determine the effect of shelf life of chicken sausages using edible film from chicken bone waste gelatin on chemical quality. This research method used a completely randomized design (CRD) with one treatment factor, namely storage time. The research treatment used edible film coating from gelatin from chicken bone waste to the storage time of chicken sausages. The data obtained were analyzed statistically using analysis of variance (ANOVA). The research observation parameters included the test of the Ph value, water content, protein content and fat content. The results showed that the use of edible film from gelatin from chicken bone waste to the storage time of chicken sausages for 9 days had a significant effect on the pH value, moisture content, protein content and, fat content. KJITRO (Jurnal Ilmu dan Teknologi Peternakan Tropis) is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  JITRO (Jurnal Ilmu dan Teknologi Peternakan Tropis) is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  eyJITRO (Jurnal Ilmu dan Teknologi Peternakan Tropis) is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  wJITRO (Jurnal Ilmu dan Teknologi Peternakan Tropis) is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. 

EVALUASI TEKNIK PEMOTONGAN AYAM YANG BERBEDA TERHADAP KUALITAS MIKROBIOLOGIS DAGING”

Penelitian ini bertujuan untuk menganalisis kandungan Escherichia coli dan Coliform dalam menentukan kualitas daging yang aman untuk dikonsumsi serta menjelaskan perbedaan dalam kualitas keamanan mikrobiologis ayam broiler yang disembelih secara tradisional dan modern. Penelitian ini telah terlaksana dari bulan Februari sampai Maret 2024 yang dimulai dari pengambilan sampel daging ayam hingga pengujian kandungan Escherichia coli dan Coliform yang dilakukan di Balai Besar Veteriner Kulon Progo, Yogyakarta. Penelitian ini menggunakan 6 sampel yang terdiri dari 3 sampel daging ayam RPA Tradisional dan 3 sampel daging ayam RPA Modern dengan berat sampel 250 gram per sampel. Hasil analisis Independet Sample T-Test menunjukan hasil Escherichia coli tidak signifikan (P 0.05). Kesimpulan dari penelitian ini adalah bahwa tidak ada perbedaan yang signifikan terkait dengan kandungan Escherichia coli pada RPA Tradisional dan RPA Modern, serta terdapat perbedaan yang signifikan terkait dengan kandungan Coliform pada RPA Tradisional dan RPA Modern. Hasil produksi daging dari kedua RPA masih tetap bisa dikonsumsi asalkan dengan cara yang benar dan matang sempurna.

A real-time biosensor system in vivo using luciferase as an intracellular reporter for the functional analysis of anti-cancer compounds based on Hsp70 inhibition

Introduction:Defective apoptosis (programmed cell death) is a major contributor to the process of cancer progression and metastasis. Over the last decade, new drug compounds were designed to enhance apoptosis in the tumor cells. Hsp70 chaperone is an anti-apoptotic protein and its inhibitors serve as suitable compounds in cancer treatment strategies. A number of different techniques such as x-ray crystallography are available for studying inhibitory effects of apoptosis-inducing compounds on Hsp70 activity. These techniques are expensive and time-consuming because of long sample preparation steps prior to analysis, and the functional analysis of new compounds synthesized cannot be examined in vivo. In order to overcome these problems, for the first time, a rapid, sensitive and inexpensive real-time biosensor system in vivo is introduced using intracellular reporter. In real-time biosensor system, because of the availability of a rapid and sensitive activity assay using biolominometer in vivo without cell lysis, luciferase has been considered as proper candidate. Methods and Results:Co-transformation of the cells was carried out with two expression vectors containing Hsp70 and firefly luciferase, and the assessment of new drug compounds on Hsp70 activity under the stress conditions was evaluated. Our result showed that Hsp70 plays a crucial role in suppressing inactivation of luciferase in vivo during heat-treatment. On the other hand, the activity of heat-inactivated luciferase was approximately regained in cells expressing Hsp70 after incubation at room temperature for 60 min. According to the results, the reactivation of thermally inactivated luciferase was inhibited in the cells by treating with Ver-155008 and PFT-μ compounds (as apoptosis-inducing compounds) with IC50 of 124 and 384 μM, respectively. Also, the sensitivity of this method for detecting Ver-155008 and PFT-μ compounds was as low as 8 and 23 μM, respectively, and it showed no response to doxorubicin anti-cancer drug which binds to DNA, and used as control. Conclusions:Real-time biosensor system using luciferase can be a class of in vivo biochemical tests used for simple, rapid and sensitive detection of apoptosis-inducing compounds based on Hsp70 inhibition to facilitate clinical and therapeutic studies in the years to come

Renewable Energy Trading in Real Time Using Simulated Clients and Energy Markets

Ph.D