My Moonshadow and I

February 10 Waiting on hold with eBay customer service because I won a bid for a butterfly shirt. My winning bid was $0.32. I didn’t realize I’d won because I wasn’t very invested and apparently a month passed and an unpaid item case was opened against me. When I go on the website to try to pay I get an error message. This is why I’m on hold with eBay customer service. I’m now being helped by a person named Justin. The item description is “Eyeshadow Long Sleeve Blue Butterfly V Neck Shirt.” The call ended abruptly. A disconnection. Now I’m calling back again. Now I’m talking to someone named Camille. There are other people in the background who are clearly also talking to other customers from a call center. There was a strange squeaking/beeping sound which reminded me of a rubber chicken being squeezed. February 13 I want to write a poem about how we have mirrors in every bathroom. March 14 Been on the verge of tears today and finally cried a little. Oliver looked up at me very concerned, I never cry. The last time I cried was when Oliver was missing. Listening to folk music, no matter if I know the song, especially now, makes me cry. March 16 This evening Kullan cooked vegetable curry with rice which Olive and I ate after our sunset walk. We took turns reading Ram Dass quotes out loud to each other as we ate. April 2 I sometimes feel immense excitement and gratitude that I can write anything I want or draw anything I want at any time. Not the freedom of expression but the intense and profound beauty at the fact I can extend myself into these forms of manifestation. I also feel extreme comfort in remembering that the world exists, anyone who has ever lived dies, we all share painful experiences no matter how isolating they may feel. April 12 Every person that has lived on this planet has lived under the same moon that I live under

Assessing Farmers’ Diverse Preferences and Expectations for Tailoring Food and Nutrition Security Interventions in Southeastern Madagascar

Background Severe food and nutrition insecurity persists in Madagascar. The Atsimo Atsinanana region is among the most affected areas due to elevated poverty rates and low levels of resilience to frequent shocks. Implementing food and nutrition security (FNS) interventions could help to improve this situation, but to be effective and sustainable, intervention packages must fit the local context. Objectives To identify locally suitable options, this study assessed the perceptions of local communities in rural Atsimo Atsinanana toward a range of FNS intervention options. Methods We held 12 gender-disaggregated workshops with 80 prospective beneficiaries of an FNS project, from inland and coastal parts of the region. Preferences were elicited for 14 potential FNS interventions. Next, through participatory ex ante impact assessment, participants ranked 8 impact criteria and individually estimated expected impacts of all intervention options on these criteria. Results Overall, participants preferred interventions targeting on-farm crop, vegetable, and livestock production. Income and food self-sufficiency were ranked as the highest intervention priorities. However, intervention preferences differed by gender and geographic location. Whereas preferences for interventions targeting dietary habits were weak across genders, women had relatively stronger preferences for these interventions than men. This shows that collecting gender-disaggregated preferences can enable more gender-sensitive choice of interventions. Preferences also reflected local livelihoods, as more market-oriented coastal sites showed stronger interest in income generation than more subsistence-oriented inland sites. The ex ante impact assessments highlight positive and negative expectations for most interventions, with increased labor burden being the most prominent negative impact overall. Conclusions The findings suggest that participatory, multidimensional impact assessments before project implementation can support development stakeholders in tailoring intervention packages, considering 1) local and gendered preferences and 2) trade-offs among development objectives.Peer Reviewe

Physical activity and exercise in dementia : an umbrella review of intervention and observational studies

Background: Dementia is a common condition in older people. Among the potential risk factors, increasing attention has been focused on sedentary behaviour. However, synthesizing literature exploring whether physical activity/exercise can affect health outcomes in people with dementia or with mild cognitive impairment (MCI) is still limited. Therefore, the aim of this umbrella review, promoted by the European Geriatric Medicine Society (EuGMS), is to understand the importance of physical activity/exercise for improving cognitive and non-cognitive outcomes in people with dementia/MCI. Methods: Umbrella review of systematic reviews (SR) (with or without meta-analyses) of randomized controlled trials (RCTs) and observational (prospective and case-control in people with MCI) studies based on a systematic literature search in several databases. The certainty of evidence of statistically significant outcomes attributable to physical activity/exercise interventions was evaluated using Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach. Results: Among 1,160 articles initially evaluated, 27 systematic reviews (4 without meta-analysis) for a total of 28,205 participants with dementia/MCI were included. No observational study on physical activity/exercise in MCI for preventing dementia was included. In SRs with MAs, physical activity/exercise was effective in improving global cognition in Alzheimer’s disease and in all types of dementia (very low/low certainty of evidence). Moreover, physical activity/ exercise significantly improved global cognition, attention, executive function, and memory in MCI, with a certainty of evidence varying from low to moderate. Finally, physical activity/exercise improved non-cognitive outcomes in people with dementia including falls and neuropsychiatric symptoms. SRs, without meta-analysis, corroborated these results. Conclusions: Supported by very low to moderate certainty of evidence, physical activity/exercise has a positive effect on several cognitive and non-cognitive outcomes in people with dementia and MCI, but RCTs, with low risk of bias/confounding, are still needed to confirm these findings

Drivers of food and nutrition security during the lean period in southeastern Madagascar

The rural population in southeastern Madagascar faces widespread poverty and weak resilience to frequent climate shocks, both contributing to severe food and nutrition insecurity. For effective policy responses and tailored interventions, development stakeholders need to know which factors determine household food and nutrition security status. In particular, the relative contributions of on-farm production diversity versus cash income are of importance since they would suggest different intervention priorities. We collected survey data on household food security and women's diets during the lean season, where improvements are most needed, as well as on households' agricultural activities, market participation, and sources of cash income from 413 randomly sampled households. Regression results suggest that frequent market participation and cash income from non-farm self-employment improve food security, but on-farm diversity does not. Other determinants of household food security include livestock assets and maternal education. Women's dietary diversity, in contrast, is associated only with market participation. The importance of market participation for food and nutrition security reflects the situation in the lean period, when farm production is low and many households' food supply relies, in part, on market purchases enabled by non-agricultural income. Our findings imply that a focus on developing food markets and enabling opportunities for income generation are likely viable strategies for improving the rural food and nutrition security situation during the lean period in southeastern Madagascar. Relatively food- and nutrition-secure households are characterized by their ability to mobilize cash (e.g., from non-farm self-employment) to purchase food

A mitotic recombination map proximal to the APC locus on chromosome 5q and assessment of influences on colorectal cancer risk

Mitotic recombination is important for inactivating tumour suppressor genes by copy-neutral loss of heterozygosity (LOH). Although meiotic recombination maps are plentiful, little is known about mitotic recombination. The APC gene (chr5q21) is mutated in most colorectal tumours and its usual mode of LOH is mitotic recombination.

An Ideal Society? Neighbors of Diverse Origins Interact to Create and Maintain Complex Mini-Organs in the Skin

Hair follicles are the focus of this primer on mammalian skin development

SARS-CoV-2 N501Y Introductions and Transmissions in Switzerland from Beginning of October 2020 to February 2021—Implementation of Swiss-Wide Diagnostic Screening and Whole Genome Sequencing

The rapid spread of the SARS-CoV-2 lineages B.1.1.7 (N501Y.V1) throughout the UK, B.1.351 (N501Y.V2) in South Africa, and P.1 (B.1.1.28.1; N501Y.V3) in Brazil has led to the definition of variants of concern (VoCs) and recommendations for lineage specific surveillance. In Switzerland, during the last weeks of December 2020, we established a nationwide screening protocol across multiple laboratories, focusing first on epidemiological and microbiological definitions. In January 2021, we validated and implemented an N501Y-specific PCR to rapidly screen for VoCs, which are then confirmed using amplicon sequencing or whole genome sequencing (WGS). A total of 13,387 VoCs have been identified since the detection of the first Swiss case in October 2020, with 4194 being B.1.1.7, 172 B.1.351, and 7 P.1. The remaining 9014 cases of VoCs have been described without further lineage specification. Overall, all diagnostic centers reported a rapid increase of the percentage of detected VOCs, with a range of 6 to 46% between 25 to 31 of January 2021 increasing towards 41 to 82% between 22 to 28 of February. A total of 739 N501Y positive genomes were analysed and show a broad range of introduction events to Switzerland. In this paper, we describe the nationwide coordination and implementation process across laboratories, public health institutions, and researchers, the first results of our N501Y-specific variant screening, and the phylogenetic analysis of all available WGS data in Switzerland, that together identified the early introduction events and subsequent community spreading of the VoCs