Utilización del gen Alt a 1 para la detección de Alternaria spp. en productos hortofrutícolas mediante una técnica de PCR

Se ha desarrollado una técnica de PCR para la detección de Alternaria spp. En productos hortofrutícolas, empleando cebadores que amplifican un fragmento de 195 pb del gen Alt a 1. El límite de detección de la técnica desarrollada fue de 102 ufc/ml, tanto en medio de cultivo como en pulpa de tomate.A polymerase chain reaction (PCR) method, based on oligonucleotide primers targeting the Alt a 1 gene, has been developed for the specific identification of Alternaria spp. In vegetables. The limit of detection of the method was 102 cfu/ml either in culture or tomato paste

Magnetic Instabilities and Phase Diagram of the Double-Exchange Model in Infinite Dimensions

Dynamical mean-field theory is used to study the magnetic instabilities and phase diagram of the double-exchange (DE) model with Hund's coupling J_H >0 in infinite dimensions. In addition to ferromagnetic (FM) and antiferromagnetic (AF) phases, the DE model supports a broad class of short-range ordered (SRO) states with extensive entropy and short-range magnetic order. For any site on the Bethe lattice, the correlation parameter q of a SRO state is given by the average q=, where theta_i is the angle between any spin and its neighbors. Unlike the FM (q=0) and AF (q=1) transitions, the transition temperature of a SRO state (T_{SRO}) with 0<q<1 cannot be obtained from the magnetic susceptibility. But a solution of the coupled Green's functions in the weak-coupling limit indicates that a SRO state always has a higher transition temperature than the AF for all fillings p<1 and even than the FM for 0.26\le p \le 0.39. For 0.39<p<0.73, where both the FM and AF phases are unstable for small J_H, a SRO phase has a non-zero T_{SRO} except close to p=0.5. As J_H increases, T_{SRO} eventually vanishes and the FM dominates. For small J_H, the T=0 phase diagram is greatly simplified by the presence of the SRO phase. A SRO phase is found to have lower energy than either the FM or AF phases for 0.26\le p0 but appears for J_H\neq 0. For p near 1, PS occurs between an AF with p=1 and either a SRO or a FM phase. The stability of a SRO state at T=0 can be understood by examining the interacting DOS,which is gapped for any nonzero J_H in an AF but only when J_H exceeds a critical value in a SRO state.Comment: 38 pages, 11 figures, submitted to New Journal of Physic

Medidas do músculo Longissimus lumborum e espessura de gordura da carcaça de cordeiros Santa Inês alimentados com níveis de feno de erva-sal (Atriplex nummularia L.) e concentrado.

De todos os componentes da carcaça, a gordura é o que apresenta maior variação quantitativa e qualitativa, podendo constituir um fator de valorização ou depreciação da carcaça e da carne, de acordo com as preferências dos consumidores. Os objetivos deste trabalho foram avaliar as medidas do músculo Longissimus lumborum, área de olho de lombo (AOL) e espessuras de gordura mínima e máxima da carcaça de cordeiros Santa Inês alimentados com 30, 40, 50 e 60% de feno de erva-sal associado a alimento concentrado. Utilizou-se 32 cordeiros castrados, com peso corporal inicial de 22 kg e confinados por 60 dias, quando foram abatidos. A profundidade do músculo Longissimus lumborum (medida B), espessuras mínima (EG) e máxima (GR) de gordura e AOL foram reduzidas à medida que houve aumento do nível de feno de erva-sal na dieta. Os níveis de feno de erva-sal estudados não proporcionaram espessura mínima de gordura subcutânea recomendada para carcaças ovinas, de 3,0 mm.Zootec 2011

Equilibrium existence in infinite horizon economies

In sequential economies with finite or infinite-lived real assets in positive net supply, we introduce constraints on the amount of borrowing in terms of the market value of physical endowments. We show that, when utility functions are either unbounded and separable in states of nature or separable in commodities, these borrowing constraints not only preclude Ponzi schemes but also induce endogenous Radner bounds on short-sales. Therefore, we obtain existence of equilibrium. Moreover, equilibrium also exists when both assets are numéraire and utility functions are quasilinear in the commodity used as numéraire.info:eu-repo/semantics/publishedVersio

The Repetitive Cytoskeletal Protein H49 of Trypanosoma cruzi Is a Calpain-Like Protein Located at the Flagellum Attachment Zone

Background: Trypanosoma cruzi has a single flagellum attached to the cell body by a network of specialized cytoskeletal and membranous connections called the flagellum attachment zone. Previously, we isolated a DNA fragment (clone H49) which encodes tandemly arranged repeats of 68 amino acids associated with a high molecular weight cytoskeletal protein. in the current study, the genomic complexity of H49 and its relationships to the T. cruzi calpain-like cysteine peptidase family, comprising active calpains and calpain-like proteins, is addressed. Immunofluorescence analysis and biochemical fractionation were used to demonstrate the cellular location of H49 proteins.Methods and Findings: All of H49 repeats are associated with calpain-like sequences. Sequence analysis demonstrated that this protein, now termed H49/calpain, consists of an amino-terminal catalytic cysteine protease domain II, followed by a large region of 68-amino acid repeats tandemly arranged and a carboxy-terminal segment carrying the protease domains II and III. the H49/calpains can be classified as calpain-like proteins as the cysteine protease catalytic triad has been partially conserved in these proteins. the H49/calpains repeats share less than 60% identity with other calpain-like proteins in Leishmania and T. brucei, and there is no immunological cross reaction among them. It is suggested that the expansion of H49/calpain repeats only occurred in T. cruzi after separation of a T. cruzi ancestor from other trypanosomatid lineages. Immunofluorescence and immunoblotting experiments demonstrated that H49/calpain is located along the flagellum attachment zone adjacent to the cell body.Conclusions: H49/calpain contains large central region composed of 68-amino acid repeats tandemly arranged. They can be classified as calpain-like proteins as the cysteine protease catalytic triad is partially conserved in these proteins. H49/calpains could have a structural role, namely that of ensuring that the cell body remains attached to the flagellum by connecting the subpellicular microtubule array to it.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Beca Presidente de la Republica-ChileUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, São Paulo, BrazilUniv Antofagasta, Lab Bioquim, Dept Biomed, Antofagasta, ChileUniv Bandeirante São Paulo, São Paulo, BrazilUniv Brasilia, Dept Biol Celular, Inst Biol, Brasilia, DF, BrazilFiocruz MS, Ctr Pesquisa Rene Rachou CPqRR, Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, São Paulo, BrazilWeb of Scienc

Emerging and recurring diseases in cetaceans worldwide and the role of environmental stressors. Scientific Committee Document SC/60/DW5, International Whaling Commission, June 2008, Santiago, Chile

Emerging and recurring infectious diseases known or suspected to have the potential to significantly impact cetacean populations, and possible synergistic effects of environmental factors are reviewed. Cetacean morbilliviruses and papillomaviruses and brucellosis may affect population densities through high mortality rates or interference with reproduction. Evidence is available for the role of environmental factors in the emergence/recurrence and severity of at least six infectious conditions i.e. lobomycosis, toxoplasmosis, tattoo skin disease, generalized bacterial infections, miscellaneous skin diseases and morbillivirus epizootics. Other micro-parasites of potential importance include rhabdo-, herpes- and parainfluenza-viruses as well as Helicobacter spp., Streptococcus spp., Salmonella spp. and Mycobacterium marinum. The population impact and aetiology of newly emerging skin diseases in South America are unknown and represent a cause of concern

Land and Peace in Colombia: FFP Methodology for Field Data Collection and Data Handling

Effective land administration is an essential step on the road to peace in Colombia. The Colombian government plans to have a complete nation-wide land tenure coverage within seven years. The traditional approach to land administration in Colombia is not up to this policy challenge: the pace is too slow, the costs too high, the procedures too complex. Fast and effective land administration is essential for the implementation of the Reforma Rural Integral of the Peace Agreements, and to maintain public confidence in the peace process. The Netherlands contributes to the implementation of the peace agreements by focussing on fast and effective land administration, together with the Colombian institutions. This paper presents a methodology for a project where fit-for-purpose Land Administration is tested at scale – after a successful field test. The test will be done in two pilot areas. These are participatory and integrated pilots, leading to cadastral maps and, more important, to land titles whenever the legal framework and institutional cooperation allows for land regularization and adjudication

The QUIJOTE experiment: project overview and first results

QUIJOTE (Q-U-I JOint TEnerife) is a new polarimeter aimed to characterize the polarization of the Cosmic Microwave Background and other Galactic and extragalactic signals at medium and large angular scales in the frequency range 10-40 GHz. The multi-frequency (10-20~GHz) instrument, mounted on the first QUIJOTE telescope, saw first light on November 2012 from the Teide Observatory (2400~m a.s.l). During 2014 the second telescope has been installed at this observatory. A second instrument at 30~GHz will be ready for commissioning at this telescope during summer 2015, and a third additional instrument at 40~GHz is now being developed. These instruments will have nominal sensitivities to detect the B-mode polarization due to the primordial gravitational-wave component if the tensor-to-scalar ratio is larger than r=0.05.Comment: To appear in "Highlights of Spanish Astrophysics VIII", Proceedings of the XI Scientific Meeting of the Spanish Astronomical Society, Teruel, Spain (2014