Desnaturalización de alta resolución para estudio de marcadores moleculares asociados a obesidad

El objetivo del presente trabajo fue determinar la utilidad de la técnica de “High Resolution Melting” (HRM) como método para identificar variantes en genes asociados al desarrollo de obesidad en niños

Diabetes mellitus and sensorineural hearing loss: is there an association? Baseline of the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil)

OBJECTIVES: Although several studies have investigated the effects of diabetes on hearing loss, the relationship between these two conditions remains unclear. Some studies have suggested that diabetes may cause sensorineural hearing loss, whereas others have failed to find an association. The biggest challenge in investigating the association between diabetes and hearing loss is the presence of confounding variables and the complexity of the auditory system. Our study investigated the association between diabetes and sensorineural hearing loss. We evaluated the influence of time from diabetes diagnosis on this association after controlling for age, gender, and hypertension diagnosis and excluding those subjects with exposure to noise. METHODS: This cross-sectional study evaluated 901 adult and elderly Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) participants from São Paulo, Brazil who underwent audiometry testing as part of ELSA-Brasil’s baseline assessment. RESULTS: Hearing thresholds and speech test results were significantly worse in the group with diabetes than in the group without diabetes. However, no significant differences were found between participants with and without diabetes after adjusting for age, gender, and the presence of hypertension. Hearing thresholds were not affected by occupational noise exposure in the groups with and without diabetes. In addition, no association between the duration of diabetes and hearing thresholds was observed after adjusting for age, gender, and hypertension. CONCLUSION: We found no association between the duration of diabetes and worse hearing thresholds after models were adjusted for age, gender, and the presence of hypertension

Nutritional and functional evaluation of inula crithmoides and mesembryanthemum nodiflorum grown in different salinities for human consumption

The nutritional composition and productivity of halophytes is strongly related to the biotic/abiotic stress to which these extremophile salt tolerant plants are subjected during their cultivation cycle. In this study, two commercial halophyte species (Inula crithmoides and Mesembryanthemum nodiflorum) were cultivated at six levels of salinity using a soilless cultivation system. In this way, it was possible to understand the response mechanisms of these halophytes to salt stress. The relative productivity decreased from the salinities of 110 and 200 mmol L−1 upwards for I. crithmoides and M. nodiflorum, respectively. Nonetheless, the nutritional profile for human consumption remained balanced. In general, I. crithmoides vitamin (B1 and B6) contents were significantly higher than those of M. nodiflorum. For both species, β-carotene and lutein were induced by salinity, possibly as a response to oxidative stress. Phenolic compounds were more abundant in plants cultivated at lower salinities, while the antioxidant activity increased as a response to salt stress. Sensory characteristics were evaluated by a panel of culinary chefs showing a preference for plants grown at the salt concentration of 350 mmol L−1. In summary, salinity stress was effective in boosting important nutritional components in these species, and the soilless system promotes the sustainable and safe production of halophyte plants for human consumption.CCMAR/Multi/04326/201, MED/UIDB/05183/202, SFRH/BD/140143/2018, SFRH/BD/149398/2019info:eu-repo/semantics/publishedVersio

Specific gravity and shrinkage of wood of three species of eucalypts for furniture

A utiliza\ue7\ue3o da madeira de \ue1rvores de esp\ue9cies de reflorestamento pela ind\ufastria moveleira tem apresentado tend\ueancia de crescimento nos \ufaltimos anos. Nesse sentido, no presente trabalho foram determinadas a massa espec\uedfica b\ue1sica e varia\ue7\ue3o dimensional da madeira de \ue1rvores de Eucalyptus grandis , Eucalyptus dunnii e Eucalyptus urophylla , de 18 anos, para a aplica\ue7\ue3o como componentes de madeira maci\ue7a em movelaria. Os ensaios laboratoriais para a avalia\ue7\ue3o da massa espec\uedfica b\ue1sica e da varia\ue7\ue3o dimensional da madeira nos sentidos radial e longitudinal do tronco das \ue1rvores de eucaliptos foram realizados de acordo com a NBR 7190/96. Os resultados da an\ue1lise da madeira das esp\ue9cies de eucalipto foram submetidos ao Teste de Homogeneidade, An\ue1lise de Vari\ue2ncia, Teste de Tukey e Correla\ue7\ue3o de Pearson e comparados com o desempenho da madeira de sucupira ( Bowdichia nitida ) e de cumaru ( Dipteryx odorata ) utilizadas na ind\ufastria moveleira. Verificou-se para a madeira de Eucalyptus grandis menor valor de varia\ue7\ue3o dimensional, sendo a mais indicada para componentes de m\uf3veis que exigem elevada estabilidade dimensional, al\ue9m de pe\ue7as de maior superf\uedcie. A madeira dessa esp\ue9cie apresentou \uedndice de varia\ue7\ue3o dimensional compat\uedvel com o de esp\ue9cies nativas utilizadas na ind\ufastria moveleira. Verificou-se, ainda, varia\ue7\ue3o radial das propriedades da madeira, com alta correla\ue7\ue3o entre a massa espec\uedfica b\ue1sica e a varia\ue7\ue3o dimensional. No sentido longitudinal, a base do tronco das \ue1rvores de eucalipto mostrou-se com a regi\ue3o de madeira de maior estabilidade dimensional.The utilization of wood from reforested species by the furniture industry is a recent trend. Thus, the present study determined the specific gravity and shrinkage of wood of 18-year-old Eucalyptus grandis , Eucalyptus dunnii and Eucalyptus urophylla , for use as components in solid wood furniture making. The tests to evaluate the specific gravity and shrinkage of wood in the radial and axial variation of the eucalyptus trees were performed according to NBR 7190/96. The results of the analysis of wood from eucalypt species were subjected to the Homogeneity Test, ANOVA, Tukey and Pearson correlation and compared to the performance of sucupira wood ( Bowdichia nitida ) and cumaru wood ( Dipteryx odorata ), often used in the furniture industry. The following results were found: Eucalyptus grandis had a lower value of shrinkage, being more suitable for furniture components that require high dimensional stability, as well as parts of larger surface. The wood of this species showed a rate of dimensional variation compatible with the native species used in the furniture industry. The radial variation of the wood was also verified, and a high correlation between specific gravity and shrinkage was found. Longitudinally, the base of the trunk of the eucalyptus trees was shown to be the region of greatest dimensional stability

Analysis of growth, yield and control of Cophes notaticeps and Polyphagotarsonemus latus in Jatropha curcas plants under different doses of silicon

The present study aims to evaluate the effects of Si on growth, yield and resistance of Jatropha curcas plants to Cophes notaticeps and Polyphagotarsonemus latus. The study was carried out in two consecutive years with J. curcas plants with 3 years old in 3x2 m spacing. The assays were set up with randomized complete block design with five concentrations (0, 10, 20, 40 and 80 mM L-1) and five replications. It was set up another experiment in a greenhouse following completely randomized factorial design 5x2 with five silicon concentrations (0, 10, 20, 40 and 80 mM L-1) and two water supply: daily irrigation with water volumes according to 50% and 100% of evapotranspiration, six replications and one plant per plot. The present study allows clarifying that J. curcas plants are non-accumulating plants, for accumulating less than 1% of silicon in the leaves. The low accumulation of silicon in the leaves did not significantly inferred in growth of J. curcas, however, the reduction of specific leaf area possibly occurred by the formation of a thin silica layer and reduced the yield by the decrease sunlight absorption. The silicon did not mitigated the damages by Cophes notaticeps and Polyphagotarsonemus latus by the low ability of the plant to accumulate silicon, however, is possible to affirm that the injuries severity by Cophes notaticeps is proportional to stem diameter