High-velocity hot CO emission close to Sgr A*: Herschel/HIFI submillimeter spectral survey toward Sgr A*

The properties of molecular gas, the fuel that forms stars, inside the cavity of the circumnuclear disk (CND) are not well constrained. We present results of a velocity-resolved submillimeter scan (~480 to 1250 GHz}) and [CII]158um line observations carried out with Herschel/HIFI toward Sgr A*; these results are complemented by a ~2'x2' CO (J=3-2) map taken with the IRAM 30 m telescope at ~7'' resolution. We report the presence of high positive-velocity emission (up to about +300 km/s) detected in the wings of CO J=5-4 to 10-9 lines. This wing component is also seen in H2O (1_{1,0}-1_{0,1}) a tracer of hot molecular gas; in [CII]158um, an unambiguous tracer of UV radiation; but not in [CI]492,806 GHz. This first measurement of the high-velocity CO rotational ladder toward Sgr A* adds more evidence that hot molecular gas exists inside the cavity of the CND, relatively close to the supermassive black hole (< 1 pc). Observed by ALMA, this velocity range appears as a collection of CO (J=3-2) cloudlets lying in a very harsh environment that is pervaded by intense UV radiation fields, shocks, and affected by strong gravitational shears. We constrain the physical conditions of the high positive-velocity CO gas component by comparing with non-LTE excitation and radiative transfer models. We infer T_k~400 K to 2000 K for n_H~(0.2-1.0)x10^5 cm^-3. These results point toward the important role of stellar UV radiation, but we show that radiative heating alone cannot explain the excitation of this ~10-60 M_Sun component of hot molecular gas inside the central cavity. Instead, strongly irradiated shocks are promising candidates.Comment: Accepted for publication in A&A Letters ( this v2 includes corrections by language editor

Retromer deficiency in Tauopathy models enhances the truncation and toxicity of Tau.

Alteration of the levels, localization or post-translational processing of the microtubule associated protein Tau is associated with many neurodegenerative disorders. Here we develop adult-onset models for human Tau (hTau) toxicity in Drosophila that enable age-dependent quantitative measurement of central nervous system synapse loss and axonal degeneration, in addition to effects upon lifespan, to facilitate evaluation of factors that may contribute to Tau-dependent neurodegeneration. Using these models, we interrogate the interaction of hTau with the retromer complex, an evolutionarily conserved cargo-sorting protein assembly, whose reduced activity has been associated with both Parkinson’s and late onset Alzheimer’s disease. We reveal that reduction of retromer activity induces a potent enhancement of hTau toxicity upon synapse loss, axon retraction and lifespan through a specific increase in the production of a C-terminal truncated isoform of hTau. Our data establish a molecular and subcellular mechanism necessary and sufficient for the depletion of retromer activity to exacerbate Tau-dependent neurodegeneration.post-print2287 K

Scalable Synthesis of 5,11-diethynylated Indeno[1,2-\u3cem\u3eb\u3c/em\u3e]fluorene-6,12-diones and Exploration of Their Solid State Packing

We report a new synthetic route to 5,11-disubstituted indeno[1,2-b]fluorene-6,12-diones that is amenable to larger scale reactions, allowing for the preparation of gram amounts of material. With this new methodology, we explored the effects on crystal packing morphology for the indeno[1,2-b]fluorene-6,12-diones by varying the substituents on the silylethynyl groups

Nanostructure of cellulose microfibrils in spruce wood

The structure of cellulose microfibrils in wood is not known in detail, despite the abundance of cellulose in woody biomass and its importance for biology, energy, and engineering. The structure of the microfibrils of spruce wood cellulose was investigated using a range of spectroscopic methods coupled to small-angle neutron and wide-angle X-ray scattering. The scattering data were consistent with 24-chain microfibrils and favored a “rectangular” model with both hydrophobic and hydrophilic surfaces exposed. Disorder in chain packing and hydrogen bonding was shown to increase outwards from the microfibril center. The extent of disorder blurred the distinction between the I alpha and I beta allomorphs. Chains at the surface were distinct in conformation, with high levels of conformational disorder at C-6, less intramolecular hydrogen bonding and more outward-directed hydrogen bonding. Axial disorder could be explained in terms of twisting of the microfibrils, with implications for their biosynthesis

Genes Contributing to Staphylococcus aureus Fitness in Abscess- and Infection-Related Ecologies

ABSTRACT Staphylococcus aureus is a leading cause of both community- and hospital-acquired infections that are increasingly antibiotic resistant. The emergence of S. aureus resistance to even last-line antibiotics heightens the need for the development of new drugs with novel targets. We generated a highly saturated transposon insertion mutant library in the genome of S. aureus and used Tn-seq analysis to probe the entire genome, with unprecedented resolution and sensitivity, for genes of importance in infection. We further identified genes contributing to fitness in various infected compartments (blood and ocular fluids) and compared them to genes required for growth in rich medium. This resulted in the identification of 426 genes that were important for S. aureus fitness during growth in infection models, including 71 genes that could be considered essential for survival specifically during infection. These findings highlight novel as well as previously known genes encoding virulence traits and metabolic pathways important for S. aureus proliferation at sites of infection, which may represent new therapeutic targets

Characterization of organic and organomineral liquid fertilizers.

The aim of this work is to study the suitability of using different charcoals in order to produce humic-like acid. Fluorescence and UV-visible spectroscopy were applied to evaluate the humidification degree by using different index: E4/E6 (2, 8), A254/A465 (8) (UVVis spectroscopic); I400/I360 (4) and I470/I360 (4) (fluorescence spectroscopy in synchronousscan mode); A440 (5) and A4/A1 (9) (fluorescence spectroscopic in emission scan)

Non-reciprocal interactions between K+ and Na+ ions in barley (Hordeum vulgare L.)

The interaction of sodium and potassium ions in the context of the primary entry of Na+ into plant cells, and the subsequent development of sodium toxicity, has been the subject of much recent attention. In the present study, the technique of compartmental analysis with the radiotracers 42K+ and 24Na+ was applied in intact seedlings of barley (Hordeum vulgare L.) to test the hypothesis that elevated levels of K+ in the growth medium will reduce both rapid, futile Na+ cycling at the plasma membrane, and Na+ build-up in the cytosol of root cells, under saline conditions (100 mM NaCl). We reject this hypothesis, showing that, over a wide (400-fold) range of K+ supply, K+ neither reduces the primary fluxes of Na+ at the root plasma membrane nor suppresses Na+ accumulation in the cytosol. By contrast, 100 mM NaCl suppressed the cytosolic K+ pool by 47–73%, and also substantially decreased low-affinity K+ transport across the plasma membrane. We confirm that the cytosolic [K+]:[Na+] ratio is a poor predictor of growth performance under saline conditions, while a good correlation is seen between growth and the tissue ratios of the two ions. The data provide insight into the mechanisms that mediate the toxic influx of sodium across the root plasma membrane under salinity stress, demonstrating that, in the glycophyte barley, K+ and Na+ are unlikely to share a common low-affinity pathway for entry into the plant cell

Dietary intake of trans fatty acids in children aged 4–5 in Spain: The INMA cohort study

Trans fatty acid (TFA) intake has been identified as a health hazard in adults, but data on preschool children are scarce. We analyzed the data from the Spanish INMA Project to determine the intake of total, industrial and natural TFA, their main sources and the associated socio-demographic and lifestyle factors in children aged 4–5 (n = 1793). TFA intake was estimated using a validated Food Frequency Questionnaire, and multiple linear regression was used to explore associated factors. The mean daily intakes of total, industrial and natural TFA were 1.36, 0.60, and 0.71 g/day, respectively. Ten percent of the children obtained >1% of their energy intake from TFA. The main sources of industrial TFA were fast food, white bread and processed baked goods. Milk, red and processed meat and processed baked goods were the main sources of natural TFA. Having parents from countries other than Spain was significantly associated with higher natural TFA (in mg/day) intake (β 45.5) and television viewing was significantly associated with higher industrial TFA intake (β 18.3). Higher fruits and vegetables intake was significantly associated with lower intakes of all TFAs, whereas higher sweetened beverages intake was significantly associated with lower total and natural TFA intake. Thus, total and industrial TFA intake was associated with less healthy food patterns and lifestyles in Spanish preschool children