Mediating Effects of Social Support in the Association Between Problems in Childhood and Adolescence and Well‑Being in Adult Domestic Adoptees

Research concerning adulthood and adoption has gained popularity in recent years, but there are very few studies involving positive variables of adjustment. Adopted people are believed to have more behavioral and emotional problems in childhood than the general population. Previous research suggests overall continuity of functioning in later life. However, certain variables might change that continuity. Through Structural Equation Modeling analyses, this paper assesses the mediating role of social support in the well-being of 70 adult adoptees. Findings showed a well-fitted model, where problems in adolescence had a direct effect on well-being in adulthood. Additionally, we found two mediating effects: (a) social support mediated the relationship between problems in adolescence and well-being later in life; and (b) problems in adolescence mediated the relationship between problems in childhood and well-being. The main implication of these results is that social support helps develop good levels of adjustment and well-being in adulthood. Taking all this into account, it is important for adoption professionals to encourage families to support their children and to provide additional support for those adoptees needing it

Estereotipos de género en universitarios españoles sobre el riesgo psicosocial en la infancia

Recent studies have shown that gender stereotypes are not sensitive to social changes. There is a gap in how gender is treated in college. This study is part of an innovation project to improve teaching quality in university. We aim to analyze the presence of gender stereotypes related to at-risk childhood in a sample of 113 university students. Most students considered that some circumstances, like substance consumption, are more likely in boys. On the contrary, they think that looking after siblings or anxiety are more common in girls. Results show the need to incorporate the gender perspective into universities. The special needs of the at-risk girls require further research and specialized training.Estudios recientes han mostrado que los estereotipos de género no son sensibles a los cambios sociales. Hay un vacío sobre cómo el género es tratado en la educación universitaria. Este estudio se enmarca en un proyecto de innovación para la mejora de la calidad docente en la universidad. Pretendemos analizar la presencia de estereotipos de género relacionados con menores en riesgo en una muestra de 113 universitarios. La mayoría de los estudiantes considera que algunas circunstancias, como el uso de sustancias, son más probables en chicos. Por el contrario, piensan que el tener que cuidar de los hermanos o la ansiedad son más comunes en chicas. Los resultados muestran la necesidad de incorporar la perspectiva de género en las universidades. Las necesidades de las chicas en riesgo requieren más investigación y formación especializada

Psychological wellbeing in adult adoptees: current age and developmental tasks

IntroductionStudies about adult adoptees are normally focused on the differences in adjustment difficulties between them and non-adoptees. However, there has been less research about adoptees' positive and developmental adjustment in adulthood. The aim of this study is to test a model of the mediating role of adoptees' achievement of adulthood tasks in the relationship between current age and psychological wellbeing.Materials and methodsThe sample consisted of 117 adults who were adopted as children by Spanish families. Their current mean age is 28.3 years. Participants underwent an interview and completed Ryff's Psychological WellBeing Scales.ResultsFindings show that current age is directly and negatively related to psychological wellbeing [c′ = −0.039, 95% CI (−0.078, −0.001)], and the relationship between these variables is mediated by adoptees' achievement of adulthood tasks [indirect effect = 0.035, 95% CI (.014, 0.059)].DiscussionThe findings support traditional theories about transitioning to adulthood, and adds relevant information about this transition in adoptees. Moreover, this work indicates a new way of assessing adoption success, based on long-term measures and normative variables. Services providers should account for the importance of supporting young people on their life transitions and promoting their wellbeing, especially among those who started from disadvantaged contexts

El lienzo de San Antonio Abad de la iglesia de la Asunción de Nuestra Señora de Pozuelo de Aragón

Este Trabajo Fin de Grado (TFG) tiene como objetivo la realización del estudio histórico-artístico y del proceso de restauración de una pintura sobre lienzo de San Antonio Abad perteneciente a la iglesia de la Asunción de Nuestra Señora de Pozuelo de Aragón (Zaragoza). Esta obra, fechada en 1563 y firmada por un artista apellidado Espinosa, ha escondido desde el momento de su ejecución una segunda pieza sacada a la luz durante su reciente restauración: el indicador horario de un antiguo reloj mecánico del siglo XV procedente del Monasterio de Santa María de Veruela. Como complemento de las investigaciones concernientes a este hallazgo, que han dado lugar a la publicación en fecha reciente (2015) de una monografía dedicada al reloj bajomedieval, aquí nos ocuparemos del estudio de la pintura sobre lienzo renacentista

Calidad de Vida y Salud Mental en Adultos Adoptados

Ese trabajo analiza el ajuste psicológico y se comprueba un modelo teórico propuesto que relaciona la presencia de síntomas psicopatológicos con una peor calidad de vida relacionada con la salud (CVRS) de adultos adoptados. Participaron 177 adultos jóvenes adoptados (media 27.67 años). Se tomaron medidas de síntomas psicopatológicos (SCL-90), calidad de vida (SF-12), autoestima (RSES) y resiliencia (BRS). El modelo de mediación múltiple resultante indica que además de la relación directa entre psicopatología y CVRS, la resiliencia y la autoestima tienen un efecto amortiguador en esta relación. Como conclusiones, se evidencia el impacto de las dificultades en salud mental en la CVRS de personas adoptadas. La presencia de recursos psicológicos positivos, como resiliencia y autoestima, hace que dimensiones psicopatológicas como ansiedad, depresión, fobias o psicoticismo pierdan valor. Se sugiere incluir programas que potencien la resiliencia como estrategia para mejorar la salud y en los servicios de postadopción

Towards Interoperability in E-health Systems: a three-dimensional approach based on standards and semantics

Proceedings of: HEALTHINF 2009 (International Conference on Helath Informatics), Porto (Portugal), January 14-17, 2009, is part of BIOSTEC (Intemational Joint Conference on Biomedical Engineering Systems and Technologies)The interoperability problem in eHealth can only be addressed by mean of combining standards and technology. However, these alone do not suffice. An appropiate framework that articulates such combination is required. In this paper, we adopt a three-dimensional (information, conference and inference) approach for such framework, based on OWL as formal language for terminological and ontological health resources, SNOMED CT as lexical backbone for all such resources, and the standard CEN 13606 for representing EHRs. Based on tha framewok, we propose a novel form for creating and supporting networks of clinical terminologies. Additionally, we propose a number of software modules to semantically process and exploit EHRs, including NLP-based search and inference, wich can support medical applications in heterogeneous and distributed eHealth systems.This work has been funded as part of the Spanish nationally funded projects ISSE (FIT-350300-2007-75) and CISEP (FIT-350301-2007-18). We also acknowledge IST-2005-027595 EU project NeO

Immunotherapeutic synergy between anti-CD137 mAb and intratumoral administration of a cytopathic Semliki Forest virus encoding IL-12

Intratumoral injection of Semliki Forest virus encoding interleukin-12 (SFV-IL-12) combines acute expression of IL-12 and stressful apoptosis of infected malignant cells. Agonist antibodies directed to costimulatory receptor CD137 (4-1BB) strongly amplify pre-existing cellular immune responses toward weak tumor antigens. In this study, we provide evidence for powerful synergistic effects of a combined strategy consisting of intratumoral injection of SFV-IL-12 and systemic delivery of agonist anti-CD137 monoclonal antibodies (mAbs), which was substantiated against poorly immunogenic B16 melanomas (B16-OVA and B16.F10) and TC-1 lung carcinomas. Effector CD8(β)(+) T cells were sufficient to mediate complete tumor eradications. Accordingly, there was an intensely synergistic in vivo enhancement of cytotoxic T lymphocytes (CTL)-mediated immunity against the tumor antigens OVA and tyrosine-related protein-2 (TRP-2). This train of phenomena led to long-lasting tumor-specific immunity against rechallenge, attained transient control of the progression of concomitant tumor lesions that were not directly treated with SFV-IL-12 and caused autoimmune vitiligo. Importantly, we found that SFV-IL-12 intratumoral injection induces bright expression of CD137 on most tumor-infiltrating CD8(+) T lymphocytes, thereby providing more abundant targets for the action of the agonist antibody. This efficacious combinatorial immunotherapy strategy offers feasibility for clinical translation since anti-CD137 mAbs are already undergoing clinical trials and development of clinical-grade SFV-IL-12 vectors is in progress

Sensitivity to anti-Fas is independent of increased cathepsin D activity and adrenodoxin reductase expression occurring in NOS-3 overexpressing HepG2 cells

Stable overexpression of endothelial nitric oxide synthase (NOS-3) in HepG2 cells (4TO-NOS) leads to increased nitro-oxidative stress and upregulation of the cell death mediators p53 and Fas. Thus, NOS-3 overexpression has been suggested as a useful antiproliferative mechanism in hepatocarcinoma cells. We aimed to identify the underlying mechanism of cell death induced by NOS-3 overexpression at basal conditions and with anti-Fas treatment. The intracellular localization of NOS-3, the nitro-oxidative stress and the mitochondrial activity were analysed. In addition, the protein expression profile in 4TO-NOS was screened for differentially expressed proteins potentially involved in the induction of apoptosis. NOS-3 localization in the mitochondrial outer membrane was not associated with changes in the respiratory cellular capacity, but was related to the mitochondrial biogenesis increase and with a higher protein expression of mitochondrial complex IV. Nitro-oxidative stress and cell death in NOS-3 overexpressing cells occurred with the expression increase of pro-apoptotic genes and a higher expression/activity of the enzymes adrenodoxin reductase mitochondrial (AR) and cathepsin D (CatD). CatD overexpression in 4TO-NOS was related to the apoptosis induction independently of its catalytic activity. In addition, CatD activity inhibition by pepstatin A was not effective in blocking apoptosis induced by anti-Fas. In summary, NOS-3 overexpression resulted in an increased sensitivity to anti-Fas induced cell death, independently of AR expression and CatD activityThis study was supported by the Instituto de Salud Carlos III (FIS 09/00185). G. Ferrín was supported by the Networked Biomedical Research Center Hepatic and Digestive Diseases (CIBEREHD

Development of a prediction model for short-term remission of patients with Crohn’s disease treated with anti-TNF drugs

Therapy with anti-tumor necrosis factor (TNF) has dramatically changed the natural history of Crohn’s disease (CD). However, these drugs are not without adverse events, and up to 40% of patients could lose efficacy in the long term. We aimed to identify reliable markers of response to anti-TNF drugs in patients with CD. A consecutive cohort of 113 anti-TNF naive patients with CD was stratified according to clinical response as short-term remission (STR) or non-STR (NSTR) at 12 weeks of treatment. We compared the protein expression profiles of plasma samples in a subset of patients from both groups prior to anti-TNF therapy by SWATH proteomics. We identified 18 differentially expressed proteins (p ≤ 0.01, fold change ≥ 2.4) involved in the organization of the cytoskeleton and cell junction, hemostasis/platelet function, carbohydrate metabolism, and immune response as candidate biomarkers of STR. Among them, vinculin was one of the most deregulated proteins (p < 0.001), whose differential expression was confirmed by ELISA (p = 0.054). In the multivariate analysis, plasma vinculin levels along with basal CD Activity Index, corticosteroids induction, and bowel resection were factors predicting NSTR

Transient and intensive pharmacological immunosuppression fails to improve AAV-based liver gene transfer in non-human primates

BACKGROUND: Adeno-associated vectors (rAAV) have been used to attain long-term liver gene expression. In humans, the cellular immune response poses a serious obstacle for transgene persistence while neutralizing humoral immunity curtails re-administration. Porphobilinogen deaminase (PBGD) haploinsufficiency (acute intermittent porphyria) benefits from liver gene transfer in mouse models and clinical trials are about to begin. In this study, we sought to study in non-human primates the feasibility of repeated gene-transfer with intravenous administration of rAAV5 vectors under the effects of an intensive immunosuppressive regimen and to analyze its ability to circumvent T-cell immunity and thereby prolong transgene expression. METHODS: Three female Macaca fascicularis were intravenously injected with 1x1013 genome copies/kg of rAAV5 encoding the human PBGD. Mycophenolate mofetil (MMF), anti-thymocyte immunoglobulin, methylprednisolone, tacrolimus and rituximab were given in combination during 12 weeks to block T- and B-cell mediated adaptive immune responses in two macaques. Immunodeficient and immunocompetent mice were intravenously injected with 5x1012 genome copies/kg of rAAV5-encoding luciferase protein. Forty days later MMF, tacrolimus and rituximab were daily administrated to ascertain whether the immunosuppressants or their metabolites could interfere with transgene expression. RESULTS: Macaques given a rAAV5 vector encoding human PBGD developed cellular and humoral immunity against viral capsids but not towards the transgene. Anti-AAV humoral responses were attenuated during 12 weeks but intensely rebounded following cessation of the immunosuppressants. Accordingly, subsequent gene transfer with a rAAV5 vector encoding green fluorescent protein was impossible. One macaque showed enhanced PBGD expression 25 weeks after rAAV5-pbgd administration but overexpression had not been detected while the animal was under immunosuppression. As a potential explanation, MMF decreases transgene expression in mouse livers that had been successfully transduced by a rAAV5 several weeks before MMF onset. Such a silencing effect was independent of AAV complementary strand synthesis and requires an adaptive immune system. CONCLUSIONS: These results indicate that our transient and intensive pharmacological immunosuppression fails to improve AAV5-based liver gene transfer in non-human primates. The reasons include an incomplete restraint of humoral immune responses to viral capsids that interfere with repeated gene transfer in addition to an intriguing MMF-dependent drug-mediated interference with liver transgene expression