RRS James Cook Cruise JC191 19 January - 1 March 2020 Hydrographic sections from the Florida Straits to the Canaries Current across 24ºN in the Atlantic Ocean

A hydrographic section across the North Atlantic Ocean at a nominal latitude of 24°N was occupied by the RRS James Cook (cruise identifier: JC191) from 19 January to 1 March, 2020. The ship departed from Port Everglades, USA, completing a total of 135 CTD stations over the Florida Straits, the western basin, Mid-Atlantic Ridge, eastern basin and eastern boundary up to Morocco, before ending the cruise in Santa Cruz de Tenerife, Spain. The main objectives of the JC191 research expedition was to collect/measure physical-, chemical-, and biological-ocean data with the purpose of estimating heat, freshwater and carbon budgets on low frequency time scales. All CTD stations had measurements from a CTD rosette equipped with temperature, conductivity, pressure, oxygen sensors, in addition to water captured from 24 niskin bottles fired at varying intervals throughout the full depth water column. The water from the niskin bottles was analysed for dissolved oxygen, carbon (DIC/TA), nutrients, and conductivity. Water for methane (CH4), C14, C13, and pigments (filtered) was collected for onshore analysis. The CTD rosette was also equipped with 2 RBR loggers measuring conductivity, temperature and pressure (up to 6,000m), and a lowered Acoustic Doppler Current Profiler (LADCP) making full depth velocity measurements. The 135 CTD stations include 2 carbon blank stations, and 2 bulk water stations for incubations. In addition to the CTD stations, the RRS James Cook has an underway system, which includes an intake for surface water to be pumped into the water bottle annex and the deck lab; two vessel mounted ADCPs (VMADCPs). A thermosalinograph and a fluorometer, installed in the water bottle annex, continually recorded conductivity, temperature and fluorescence. Water from the CTD was collected to calibrate the ship’s underway TSG. The VMADCPs, 75Hz and 150HZ, mounted on the drop keel record ocean velocities in roughly the top 300- and 600-m, respectively. Surface carbon and methane measurements were also recorded from the underway systems, and surface meteorological variables were monitored via the meteorological sampling system and the pumped water underway system. Finally bathymetric data were recorded an EA640 echosounder and a Kongsberg EM122 multibeam, both of which are mounted on the ship’s hull. Last, 5 Deep Apex Argo floats measuring conductivity, temperature, pressure and oxygen (except for one float not equipped with an optode) were deployed in the western basin. Many of the science party also engaged in extensive outreach via blogs and social media, heightening visibility of the science teams activities to the oceanographic community and the general public. This report summarises the data collected and analysed, and the methodology used for the acquisition and processing of the data onboard the James Cook during the JC191 research expedition

Interim outcomes of mechanical thrombectomy for deep vein thrombosis from the all-comer CLOUT registry

OBJECTIVES: The multicenter, prospective, single arm CLOUT registry assesses the safety and effectiveness of the ClotTriever System (Inari Medical, Irvine, CA) for the treatment of acute and nonacute lower extremity deep vein thrombosis (DVT) in all-comer patients. Reported here are the outcomes of the first 250 patients. METHODS: All-comer patients with lower extremity DVT were enrolled, including those with bilateral DVT, those with previously failed DVT treatment, and regardless of symptom duration. The primary effectiveness end point is complete or near-complete (≥75%) thrombus removal determined by independent core laboratory-adjudicated Marder scores. Safety outcomes include serious adverse events through 30 days and clinical outcomes include post-thrombotic syndrome severity, symptoms, pain, and quality of life through 6 months. RESULTS: The median age was 62 years and 40% of patients had contraindications to thrombolytics. A range of thrombus chronicity (33% acute, 35% subacute, 32% chronic) was observed. No patients received thrombolytics and 99.6% were treated in a single session. The median thrombectomy time was 28 minutes. The primary effectiveness end point was achieved in 86% of limbs. Through 30 days, one device-related serious adverse event occurred. At 6 months, 24% of patients had post-thrombotic syndrome. Significant and sustained improvements were observed in all clinical outcomes, including the Revised Venous Clinical Severity Score, the numeric pain rating scale, and the EuroQol Group 5-Dimension Self-Report Questionnaire. CONCLUSIONS: The 6-month outcomes from the all-comer CLOUT registry with a range of thrombus chronicities demonstrate favorable effectiveness, safety, and sustained clinical improvements

POLR1B and neural crest cell anomalies in Treacher Collins syndrome type 4

PURPOSE: Treacher Collins syndrome (TCS) is a rare autosomal dominant mandibulofacial dysostosis, with a prevalence of 0.2-1/10,000. Features include bilateral and symmetrical malar and mandibular hypoplasia and facial abnormalities due to abnormal neural crest cell (NCC) migration and differentiation. To date, three genes have been identified: TCOF1, POLR1C, and POLR1D. Despite a large number of patients with a molecular diagnosis, some remain without a known genetic anomaly. METHODS: We performed exome sequencing for four individuals with TCS but who were negative for pathogenic variants in the known causative genes. The effect of the pathogenic variants was investigated in zebrafish. RESULTS: We identified three novel pathogenic variants in POLR1B. Knockdown of polr1b in zebrafish induced an abnormal craniofacial phenotype mimicking TCS that was associated with altered ribosomal gene expression, massive p53-associated cellular apoptosis in the neuroepithelium, and reduced number of NCC derivatives. CONCLUSION: Pathogenic variants in the RNA polymerase I subunit POLR1B might induce massive p53-dependent apoptosis in a restricted neuroepithelium area, altering NCC migration and causing cranioskeletal malformations. We identify POLR1B as a new causative gene responsible for a novel TCS syndrome (TCS4) and establish a novel experimental model in zebrafish to study POLR1B-related TCS

Zooplancton (rotifera y microcrustacea) y su relación con los cambios del nivel del agua en la ciénaga de ayapel, córdoba, colombia

Entre septiembre de 2004 y junio de 2005 se estudió la relación entre la estructuradel zooplancton y los cambios en el nivel del agua en la Ciénaga de Ayapel (Córdoba,Colombia) en once estaciones y seis muestreos. Mientras los rotíferos dominaron lariqueza de especies, los copépodos presentaron mayores abundancias representadosprincipalmente por nauplios y copepoditos. A pesar de que la densidad de zooplanctonno presentó diferencias estadísticamente signifi cativas en el espacio ni en el tiempo,se encontró un aumento en la densidad del zooplancton durante la fase de llenadode la ciénaga

RRS James Cook Cruise JC159 28 February - 11 April 2018. Hydrographic sections from the Brazil to the Benguela Current across 24S in the Atlantic

A Hydrographic section was occupied at a nominal latitude of 24°S in the Atlantic Ocean during March and April 2018 on Cruise JC159 of RRS James Cook. The primary objective of this cruise was to measure ocean physical, chemical and biological parameters in order to establish regional budgets of heat, freshwater and carbon, and to infer decadal variability. In addition, 371 Niskin Bottles were sampled for microplastics, reflecting increasing awareness of plastics pollution in the oceans. A total of 121 CTD/LADCP stations were conducted, including one test station and two CFC bottle blank stations. In addition to temperature, salinity and oxygen profiles from the sensors on the CTD package, water samples from a 24 x 20 litre rosette were analysed for the following parameters at all stations: salinity; dissolved oxygen; inorganic nutrients; alkalinity and dissolved inorganic carbon; CFCs. Samples were collected for shore analysis for oxygen and carbon isotopes (del-18O, del13C and del-14C). Samples were collected and filtered for pigments (shore analysis) at 44 stations and for microplastics at 45 stations. 8 Argo floats were deployed, including two Bio-PROVOR floats and 2 Deep ARVORs. In addition, samples were collected from the ships’ underway system to calibrate and compliment the data continually collected by the TSG (thermosalinograph). Full depth velocity measurements were made at every station by LADCP (Lowered Acoustic Doppler Current Profiler) mounted on the frame of the rosette. Throughout the cruise, velocity data in the upper few hundred metres of the water column were collected by the ship’s VMADCP (Vessel Mounted Acoustic Doppler Current Profiler) transducers (75Hz and 150Hz) mounted on one of the two drop keels. Meteorological variables were monitored using the onboard surface water and meteorological sampling system (SURFMET). Bathymetric data were collected using the Kongsberg EM122 multibeam system and the EA640 echo sounder. This report describes the methods used to acquire and process the data on board the ship during cruise JC159

Access Mars: Assessing Cave Capabilities Establishing Specific Solutions: Final Report

The human race has evolved, grown and expanded through the exploration of Earth. After initial steps on the Moon, our next challenge is to explore the solar system. Mars shows potential for both scientific discovery and future human settlement, and so is a prime candidate for the next leap of human exploration. Such a bold endeavor will be a driver for an unprecedented worldwide cooperative effort and the catalyst for a new era of international, intercultural and interdisciplinary human relations. Scientific and technological progress will also accelerate as mankind is ushered into a new era of space exploration. Currently proposed Mars missions have identified a number of challenges such as high levels of radiation, harsh climate and limited launch windows. Recently discovered lava tubes on Mars present potential solutions to some of these issues, but raise a variety of intriguing new challenges. These encompass not only technological and engineering considerations, but also legal, ethical and societal issues such as planetary protection and crew safety. This report assesses the feasibility of overcoming such challenges through the exploitation of Mars caves. This report reviews existing reference missions and identifies areas of further research essential for adapting mission architectures to utilize caves. Cave suitability is considered with respect to size, type, location and their potential to mitigate hazards. They are also assessed with respect to their potential for scientific work adhering to astrobiology guidelines and the search for extra-terrestrial life. This report compares surface and subsurface habitat options. Engineering challenges arising from the use of caves are addressed along with proposals for alternate architecture solutions. Mission analysis is conducted to determine the transit trajectory and define two possible mission scenarios with surface crews of 6 and 12 crew members. Different types of habitat are described and evaluated. An architecture for precursor missions is provided utilizing surface rovers, cargo delivery rovers and pressurized human transport vehicles. The implications of sub-surface operations on thermal control, communications and power systems are investigated with recommendations given. Crew selection, training methods and life support system solutions are also addressed. Literature suggests a low radiation environment within Martian caves, allowing for extended duration missions. The ACCESS Mars Team concludes that using lava tubes as human habitats is not merely a viable habitat solution for a Mars expedition, but also potentially more beneficial than proposed surface solutions

DNA damage by lipid peroxidation products: implications in cancer, inflammation and autoimmunity

Oxidative stress and lipid peroxidation (LPO) induced by inflammation, excess metal storage and excess caloric intake cause generalized DNA damage, producing genotoxic and mutagenic effects. The consequent deregulation of cell homeostasis is implicated in the pathogenesis of a number of malignancies and degenerative diseases. Reactive aldehydes produced by LPO, such as malondialdehyde, acrolein, crotonaldehyde and 4-hydroxy-2-nonenal, react with DNA bases, generating promutagenic exocyclic DNA adducts, which likely contribute to the mutagenic and carcinogenic effects associated with oxidative stress-induced LPO. However, reactive aldehydes, when added to tumor cells, can exert an anticancerous effect. They act, analogously to other chemotherapeutic drugs, by forming DNA adducts and, in this way, they drive the tumor cells toward apoptosis. The aldehyde-DNA adducts, which can be observed during inflammation, play an important role by inducing epigenetic changes which, in turn, can modulate the inflammatory process. The pathogenic role of the adducts formed by the products of LPO with biological macromolecules in the breaking of immunological tolerance to self antigens and in the development of autoimmunity has been supported by a wealth of evidence. The instrumental role of the adducts of reactive LPO products with self protein antigens in the sensitization of autoreactive cells to the respective unmodified proteins and in the intermolecular spreading of the autoimmune responses to aldehyde-modified and native DNA is well documented. In contrast, further investigation is required in order to establish whether the formation of adducts of LPO products with DNA might incite substantial immune responsivity and might be instrumental for the spreading of the immunological responses from aldehyde-modified DNA to native DNA and similarly modified, unmodified and/or structurally analogous self protein antigens, thus leading to autoimmunity

MicroMotility: State of the art, recent accomplishments and perspectives on the mathematical modeling of bio-motility at microscopic scales

Mathematical modeling and quantitative study of biological motility (in particular, of motility at microscopic scales) is producing new biophysical insight and is offering opportunities for new discoveries at the level of both fundamental science and technology. These range from the explanation of how complex behavior at the level of a single organism emerges from body architecture, to the understanding of collective phenomena in groups of organisms and tissues, and of how these forms of swarm intelligence can be controlled and harnessed in engineering applications, to the elucidation of processes of fundamental biological relevance at the cellular and sub-cellular level. In this paper, some of the most exciting new developments in the fields of locomotion of unicellular organisms, of soft adhesive locomotion across scales, of the study of pore translocation properties of knotted DNA, of the development of synthetic active solid sheets, of the mechanics of the unjamming transition in dense cell collectives, of the mechanics of cell sheet folding in volvocalean algae, and of the self-propulsion of topological defects in active matter are discussed. For each of these topics, we provide a brief state of the art, an example of recent achievements, and some directions for future research