60 research outputs found
Relationship between spermatozoa motility parameters, sperm/egg ratio, and fertilization and hatching rates in pufferfish (Takifugu niphobles)
[EN] The use of high quality gametes from both males and females during in vitro fertilization (IVF) trials is an essential step in order to achieve high fertilization and hatching rates. Although aquaculture hatcheries have focused more on egg rather than spermatozoa quality, some studies have demonstrated that sperm quantity and quality have a great influence both on fertilization/hatching success and the subsequent development of the embryo and larvae.
In this study we have demonstrated that sperm/egg ratio and sperm quality are factors strongly related to each other in the pufferfish (Takifugu niphobles). Our results suggest that both factors should be taken into account as unique interrelated elements, making possible to obtain high fertilization rates using a successful combination of small amount of high quality sperm or high amount of low quality sperm.
In addition, coefficients of correlation and determination among all the sperm motion parameters provided by a CASA system and fertilization/hatching rates were estimated for the first time in a marine species. Positive significant correlations were found in some parameters such as total and progressive motility (0.68 and 0.7 respectively). However, curvilinear velocity (VCL), straight line velocity (VSL) and average velocity (VAP) showed the highest coefficients of correlation (0.82, 0.8, and 0.81, respectively). In this respect, spermatozoa velocity appears to be a key factor in the fertilization process, especially when the number of spermatozoa per egg is limited in the aqueous environment. (C) 2013 Elsevier B.V. All rights reserved.Funded by the Spanish Ministry of Economy and Competitiveness (MINECO; AGL2010-16009). Victor Gallego has a predoctoral grant (MINECO; BES-2009-020310) and has been granted a fellowship (EEBB-I-12-05858) of the MINECO's Spanish Personnel Research Training Programme to carry out this research in the Misaki Marine Biological Station (Miura, Japan). We would like to thank to Dr. Kurokawa for the help and knowledge supplied during this study.Gallego Albiach, V.; PĂŠrez Igualada, LM.; Asturiano Nemesio, JF.; Yoshida, M. (2013). Relationship between spermatozoa motility parameters, sperm/egg ratio, and fertilization and hatching rates in pufferfish (Takifugu niphobles). Aquaculture. 416:238-243. https://doi.org/10.1016/j.aquaculture.2013.08.035S23824341
Desenvolvimento de um roteiro conceitual para a gestão da biodiversidade e dos serviços ecossistêmicos no Caribe mexicano
Coral reefs and mangroves support rich biodiversity and provide ecosystem services that range from food, recreational benefits and coastal protection services, among others. They are one of the most threatened ecosystems by urbanization processes. In this context, we developed a conceptual framework for the management of biodiversity and ecosystem services for these coastal environments. We based our workflow on two sections: âInformation baseâ and âGovernanceâ and use the Puerto Morelos Coastal region as a case study for coastal protection. Puerto Morelos is between two of the most touristic destinations of Mexico (Playa del Carmen and Cancun) that has experienced an increase of population in the past four decades resulting in an intensification of multiple threats to its ecosystems. We characterized the two ecosystems with a âManagement Unitsâ strategy. An expert-based ecosystem services matrix was also described in order to connect mangroves and coral reef ecosystems with the multiple beneficiaries. Then an ecosystem model (conceptual model and Global Biodiversity model) was developed. The conceptual model was useful in understanding the interplay processes between systems regarding the ecosystem service of âCoastal Protectionâ. The Global Biodiversity model evidenced the human-induced shifts in the biodiversity for mangrove and coral reefs ecosystems. Also, a projection for 2035 of âbestâ and âworstâ scenarios was applied using GLOBIO3. A DPSIR conceptual framework was used to analyze environmental problems regarding ecosystem services maintenance. Finally, we evaluated a set of policies associated with these ecosystems that favor coastal protection integrity. This framework facilitates the identification of the most relevant processes and controls about the provision of coastal protection service. It can also be useful to better target management actions and as a tool to identify future management needs to tackle the challenges preventing more effective conservation of coastal environments.Recifes de coral e manguezais possuem rica biodiversidade e fornecem serviços ecossistĂŞmicos, tais como, alimento, recreação, proteção costeira, entre outros. Esses ecossistemas encontram-se entre os mais ameaçados pelos processos de urbanização. Nesse contexto, desenvolvemos um roteiro conceitual para a gestĂŁo da biodiversidade e dos serviços ecossistĂŞmicos desses ambientes costeiros. Organizamos nossa sequĂŞncia de passos de trabalho em duas seçþes: âBase de informaçþesâ e âGovernançaâ e usamos a regiĂŁo costeira da cidade de Puerto Morelos (MĂŠxico) como um estudo de caso para analisar o serviço de proteção de costa. Puerto Morelos encontra-se entre dois dos destinos mais turĂsticos do MĂŠxico (Playa del Carmen e CancĂşn), e portanto sua população vem aumentando nas Ăşltimas quatro dĂŠcadas, resultando na intensificação de mĂşltiplas ameaças para os ecossistemas. Primeiramente, caracterizamos os dois ecossistemas identificando-os como âUnidades de GestĂŁoâ, detalhando seus principais componentes e processos. AtravĂŠs de uma âMatriz de serviços ecossistĂŞmicosâ, construĂda com base na opiniĂŁo de especialistas, foram sistematizados os principais serviços ecossistĂŞmicos prestados pelos manguezais e recifes de corais aos mĂşltiplos beneficiĂĄrios. Em seguida, foi desenvolvida uma modelagem do sistema (e ecossistemas) atravĂŠs de sua representação na forma de um modelo conceitual e um modelo numĂŠrico de Biodiversidade Global. O modelo conceitual facilitou a compreensĂŁo dos processos de interação entre sistemas em relação ao serviço âProteção Costeiraâ. O modelo numĂŠrico evidenciou as mudanças induzidas pelo homem na biodiversidade dos ecossistemas de manguezal e recifes de coral. AlĂŠm disso, uma projeção dos cenĂĄrios âmelhorâ e âpiorâ foi desenvolvida para 2035 usando GLOBIO3. A Estrutura conceitual DPSIR foi aplicada para analisar problemas ambientais relacionados Ă manutenção dos serviços ecossistĂŞmicos. Finalmente, avaliamos um conjunto de polĂticas pĂşblicas associadas a esses ecossistemas e que favorecem a integridade da proteção costeira. Portanto, o roteiro facilitou a identificação dos principais processos e controles para a provisĂŁo de um serviço ecossistĂŞmico. AlĂŠm disso, pode ser Ăştil para direcionar melhor as açþes de gerenciamento, bem como, uma ferramenta para identificar necessidades futuras de planejamento e gestĂŁo para enfrentar desafios que permitam uma conservação mais eficaz dos ambientes costeiros.Fil: SĂĄnchez Quinto, AndrĂŠs. Universidad Nacional AutĂłnoma de MĂŠxico; MĂŠxicoFil: Costa, Julliet Correa da. Universidade Federal de Santa Catarina; BrasilFil: Zamboni, Nadia Selene. Universidade Federal do Rio Grande do Norte; BrasilFil: Sanches, FĂĄbio H. C.. Universidade Federal de Sao Paulo; BrasilFil: Principe, Silas C.. Universidade de Sao Paulo; BrasilFil: Viotto, Evangelina del Valle. Provincia de Entre RĂos. Centro de Investigaciones CientĂficas y Transferencia de TecnologĂa a la ProducciĂłn. Universidad AutĂłnoma de Entre RĂos. Centro de Investigaciones CientĂficas y Transferencia de TecnologĂa a la ProducciĂłn. Consejo Nacional de Investigaciones CientĂficas y TĂŠcnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe. Centro de Investigaciones CientĂficas y Transferencia de TecnologĂa a la ProducciĂłn; ArgentinaFil: Casagranda, Maria Elvira. Universidad Nacional de TucumĂĄn. Instituto de EcologĂa Regional. Consejo Nacional de Investigaciones CientĂficas y TĂŠcnicas. Centro CientĂfico TecnolĂłgico Conicet - TucumĂĄn. Instituto de EcologĂa Regional; ArgentinaFil: Lima, Francisco A. da Veiga. Universidade Federal de Santa Catarina; BrasilFil: Possamai, Bianca. Universidade Federal Do Rio Grande.; BrasilFil: Faroni Perez, Larisse. Universidade Federal de Juiz de Fora; Brasi
Alignment of the CMS silicon tracker during commissioning with cosmic rays
This is the Pre-print version of the Article. The official published version of the Paper can be accessed from the link below - Copyright @ 2010 IOPThe CMS silicon tracker, consisting of 1440 silicon pixel and 15 148 silicon strip detector modules, has been aligned using more than three million cosmic ray charged particles, with additional information from optical surveys. The positions of the modules were determined with respect to cosmic ray trajectories to an average precision of 3â4 microns RMS in the barrel and 3â14 microns RMS in the endcap in the most sensitive coordinate. The results have been validated by several studies, including laser beam cross-checks, track fit self-consistency, track residuals in overlapping module regions, and track parameter resolution, and are compared with predictions obtained from simulation. Correlated systematic effects have been investigated. The track parameter resolutions obtained with this alignment are close to the design performance.This work is supported by FMSR (Austria); FNRS and FWO (Belgium); CNPq, CAPES, FAPERJ,
and FAPESP (Brazil); MES (Bulgaria); CERN; CAS, MoST, and NSFC (China); COLCIENCIAS
(Colombia); MSES (Croatia); RPF (Cyprus); Academy of Sciences and NICPB (Estonia);
Academy of Finland, ME, and HIP (Finland); CEA and CNRS/IN2P3 (France); BMBF, DFG,
and HGF (Germany); GSRT (Greece); OTKA and NKTH (Hungary); DAE and DST (India); IPM (Iran); SFI (Ireland); INFN (Italy); NRF (Korea); LAS (Lithuania); CINVESTAV, CONACYT,
SEP, and UASLP-FAI (Mexico); PAEC (Pakistan); SCSR (Poland); FCT (Portugal); JINR (Armenia, Belarus, Georgia, Ukraine, Uzbekistan); MST and MAE (Russia); MSTDS (Serbia); MICINN and CPAN (Spain); Swiss Funding Agencies (Switzerland); NSC (Taipei); TUBITAK and TAEK (Turkey); STFC (United Kingdom); DOE and NSF (USA)
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