Anti-schistosoma single-domain antibody-nanoparticles conjugate: a novel tool for diagnostic and therapeutic applications

Nanotechnology has enthused excessive expectations in recent years, particularly in the biology and biomedical fields. Carbon-coated metallic nanomagnets reveal significant physicochemical properties, which are referred to as superparamagnetism, that when designed appropriately can be utilized to generate novel diagnostic and therapeutic applications for a wide range of biologically hazardous species. Schistosomiasis is a chronic parasitic disease that can infect both humans and animals, particularly cattle. It is caused by a trematode blood fluke of the genus Schistosoma which belong to the schistosomatidae family. At least 200 million individuals are infected in 75 countries while 600 million people are exposed to infection and are potentially victim of this parasite. Schistosomiasis is still recognized as one of the most neglected disease, the current available diagnostic techniques are deficient in accuracy and are incapable of identifying the disease in its early stages. Moreover, they suffer from prolonged examination time. Besides, the poorly available therapeutic drugs started to lose their efficacy and the parasite started to develop resistance against most of them, which create an imperative need for developing novel diagnostic and therapeutic tools. The humoral immune response of the Camelidae is unique since these animals possess functional heavy-chain only antibodies in addition to the classical antibodies. Thanks to recombinant DNA technology, a series of single domain antigen binding entities can be produced against specific schistosomeâ s parasite antigens. These binders possess a number of distinctive biophysical properties that offer particular advantages in various theranostic applications. Finally, nanomagnetism was selected to represent a certain phenomena through which we demonstrated, to our knowledge for the first time, the successful construction of effective and functional bioconjugate system which is appropriate for a plethora of diagnostic and therapeutic applications. The main thrust of this research work was towards the developing of single domain antibodies against Schistosoma mansoni gut specific antigen and execratory secretory antigen. In parallel, state-of-the-art methodologies were implemented for constructing a bioconjugate system composed of sdAb and graphene-coated metallic nanomagnet tagged with a carboxyl terminal. Finally, the well-established metastable technetium-99 (99mTc) is used for labeling the conjugate for studying the possibility of active and magnetic targeting of the nano-structured system i.e. 99mTc-sdAb-Fe@C-MNP conjugates

The Incoherence of Success: Intersections of Marriage and Education in a Second-Chance Girls\u27 Education Initiative in Rural Upper Egypt

In Egypt, early-marriage is implicated as a barrier to educational access for girls living in rural areas. It is understood to impede women’s access to education and the labor market. This paper focuses on Ishraq, a second chance girls’ education initiative brought on by the Girls Education Initiative-Egypt. Using a critical poststructural conceptual framework, this paper examines how individuals associated with the Ishraq program engage in social contests concerning the relationship between marriage and education by employing a qualitative case study approach grounded in ethnographic methodological considerations. This essay argues participant’s view religious life as the single most important consideration in articulating and enacting their conceptions of community development and girl’s empowerment, providing a framework for understanding the relationship between marriage and education. These perspectives demonstrate how participants navigate certain structural realities in their lives and the strategies they employ in localizing the designed affects of the Ishraq program

A Study of DAS delays and their Impact on the Wireless Channels with Application to Indoor Localization

This research evaluates the Distributed Antenna Systems (DAS) introduced delays and their effects on the indoor channel in simulcast situations where the effect of delays is most prevalent. Different simulcast cases that form the basic building blocks are analyzed to form an understanding of the problem. Two case studies of important indoor environments are presented. Importance of improving ray tracing simulations to include propagation and DAS delays is highlighted. The paper also introduces a DAS element representation and delay mapping model and explores techniques of engineering DAS delays to optimize location estimation by ranging and RF fingerprinting to achieve E911 mandated accuracy. A brief description is introduced for a Software Defined Radio (SDR) implementation of a Correlation Channel Sounder and the possible application of channel sounding for indoor DAS. The paper suggests procedures to produce a full DAS delay profile and ways to optimize it for location estimation

Numerical study of viscous dissipation effect on free convection heat and mass transfer of MHD non-Newtonian fluid flow through a porous medium

AbstractThe problem of free convection heat with mass transfer for MHD non-Newtonian Eyring–Powell flow through a porous medium, over an infinite vertical plate is studied. Taking into account the effects of both viscous dissipation and heat source. The temperature and concentration are of periodic variation. The governing non-linear partial differential equations of this phenomenon are transformed into non-linear algebraic system utilizing finite difference method. Numerical results for the velocity, temperature and concentration distributions as well as the skin friction, heat and mass transfer are obtained and reported in tabular form and graphically for different values of physical parameters of the problem. Also, the stability condition is studied

Beyond the commonest: right lower quadrant abdominal pain is not always appendicitis

Background: Despite acute appendicitis is the most common underlying etiology of the acute abdomen, a considerable list of other pathologies should be considered; despite sharing almost the same clinical presentation. This study aimed to re-enhance the importance and sensitivity of CT scan as a solitary imaging tool, in the evaluation of patients with acute right lower abdominal pain. Patients and methods: It is a retrospective study of the last 1000 patients, referred with acute right lower abdominal pain for CT scan examination. Biphasic CT scan abdomen protocol was applied for all. Results: Cecal carcinoma was detected in three patients (0.3%), epiploic appendicitis in five (0.5%), omental infarct in two (0.2%), appendicular mucocele in two (0.2%), appendicular NET in one (0.1%), cecal diverticulitis in four (0.4%), Chron’s disease in six (0.6%), intussusception in one (0.1%) and FB in one (0.1%) and appendicular perforation in one (0.1%), acute simple appendicitis 398 (39.8%) and 577 (57.7%) were unremarkable. Conclusion: Despite acute appendicitis is the most common cause of the acute abdomen, many other surgical and non-surgical etiologies should also be considered, with a higher sensitivity of CT as a solitary diagnostic tool of imaging

Performance Comparison of Several Control Algorithms for Tracking Control of Pantograph Mechanism

A sort of parallel manipulator known as a pantograph robot mechanism was created primarily for industrial requests that required high precision and satisfied speed. While tracking a chosen trajectory profile requires a powerful controller. Because it has four active robot links and one robot passive link in place of just two links like the open chain does, it can carry more loads than the open chain robot mechanism while maintaining accuracy and stability. The calculated model for a closed chain pantograph robot mechanism presented in this paper takes into account the boundary conditions. For the purpose of simulating the dynamics of the pantograph robot mechanism, an entire MATLAB Simulink has been created. The related Simscape model had been created to verify the pantograph mathematical model that had been provided. Five alternative tracking controllers were also created and improved using the Flower Pollination (FP) algorithm. The PID controller, which is used in many engineering applications, is the first control. An enriched Fractional Order PID (FOPID) controller is the second control. The third control considers an improved Nonlinear conventional PID (NLPID) controller, and the parameters for this controller were likewise determined using (FP) optimization using the useful objective function. Model Reference Adaptive Control (MRAC) with PID Compensator is the fourth control. The Fuzzy PD+I Control is the last and final controller. A comparison of the different control methods was completed. A rectangular trajectory was chosen as the end effector of the pantograph robot\u27s position reference because it displays performance during sharp edges and provides a more accurate study. The proposed controllers were used for this task to analyse the performance. The outcomes demonstrate that the Fuzzy PD+I control outperforms the PID, FOPID, NLPID, and MRAC with PID Compensator controllers in terms of performance. In the case of the Fuzzy PD+I control, the angles end effector has a lower rise time, a satisfied settling time, and low overshoot with good precision

The effect of biventricular pacing on cardiac function after open heart surgery

Background: Temporary postoperative pacing could enhance recovery of the cardiac function. The right ventricular pacing (RV) is commonly used, but it can cause dyssynchronous contraction of both ventricles. Biventricular pacing (BV) could improve the systolic function by synchronizing the ventricular contraction. The aim of this study is to evaluate the effectiveness of biventricular pacing in improving the hemodynamics in the early postoperative period compared to other pacing modes. Methods: This is a clinical crossover trial including 50 patients who underwent open cardiac surgery in the period from September 2017 to September 2018. Mean age was 46.78± 12.09 years, and 50% were males. Temporary pacing leads were attached to the anterior wall of the right ventricle 1-2 cm paraseptally and the lateral wall of left ventricle 1-2 cm paraseptally. Each patient was paced for 3 minutes in the first 1-4 postoperative hours with 20 minutes washout period between different pacing modes. Study endpoints included cardiac output, ejection fraction (EF) and wall motion abnormality. Results: Biventricular and right ventricular pacing increased postoperative cardiac output (6.31± 1.28 and 5.2±0.72 L/min; respectively), but BV pacing was superior to RV pacing (P-value <0.001). The effect of BV pacing was more evident in patients with EF < 50% (7.27± 0.895 vs. 5.26 ± 0.634 L/min; p< 0.001). The postoperative EF improved during BV pacing (53.16± 4.71%) compared to RV pacing (49.4± 4.07%; P-value <0.001). Both BV and RV pacing were associated with less paradoxical septal wall motion abnormality (P-value <0.001). Conclusions: Temporary postoperative biventricular pacing improves hemodynamics compared to right ventricular and no pacing. Routine BV pacing is recommended especially in patients with low ejection fraction