Reduction of Petri net maintenance modeling complexity via Approximate Bayesian Computation

This paper is part of the ENHAnCE ITN project (https://www.h2020-enhanceitn.eu/) funded by the European Union's Horizon 2020 research and innovation programme under the Marie SklodowskaCurie grant agreement No. 859957. The authors would like to thank the Lloyd's Register Foundation (LRF), a charitable foundation in the U.K. helping to protect life and property by supporting engineeringrelated education, public engagement, and the application of research. The authors gratefully acknowledge the support of these organizations which have enabled the research reported in this paper.The accurate modeling of engineering systems and processes using Petri nets often results in complex graph representations that are computationally intensive, limiting the potential of this modeling tool in real life applications. This paper presents a methodology to properly define the optimal structure and properties of a reduced Petri net that mimic the output of a reference Petri net model. The methodology is based on Approximate Bayesian Computation to infer the plausible values of the model parameters of the reduced model in a rigorous probabilistic way. Also, the method provides a numerical measure of the level of approximation of the reduced model structure, thus allowing the selection of the optimal reduced structure among a set of potential candidates. The suitability of the proposed methodology is illustrated using a simple illustrative example and a system reliability engineering case study, showing satisfactory results. The results also show that the method allows flexible reduction of the structure of the complex Petri net model taken as reference, and provides numerical justification for the choice of the reduced model structure.European Commission 859957Lloyd's Register Foundation (LRF), a charitable foundation in the U.K

Acid and enzymatic fractionation of olive stones for ethanol production using Pachysolen tannophilus

Olive stones are an abundant lignocellulose material in the countries of the Mediterranean basin that could be transformed to bioethanol by biochemical pathways. In this work, olive stones were subjected to fractionation by means of a high-temperature dilute-acid pretreatment followed by enzymatic hydrolysis of the pretreated solids. The hydrolysates obtained in these steps were separately subjected to fermentation with the yeast Pachysolen tannophilus ATCC 32691. Response surface methodology with two independent variables (temperature and reaction time) was applied for optimizing D-xylose production from the raw material by dilute acid pretreatment with 0.01 M sulfuric acid. The highest D-xylose yield in the liquid fraction was obtained in the pretreatment at 201 C for 5.2 min. The inclusion of a detoxification step of the acid prehydrolysate, by vacuum distillation, allowed the fermentation of the sugars into ethanol and xylitol. The enzymatic hydrolysis of the pretreated solids was solely effective when using high enzyme loadings, thus leading to easily fermentable hydrolysates into ethanol. The mass macroscopic balances of the overall process illustrated that the amount of inoculum used in the fermentation of the acid prehydrolysates strongly aected the ethanol and xylitol yields.Junta de Andalucía 01272/2005, AGR/650

Tunnelling in quantum superlattices with variable lacunarity

Quantum fractal superlattices are microelectronic devices consisting of a series of thin layers of two semiconductor materials deposited alternately on each other over a substrate following the rules of construction of a fractal set, here, a symmetrical polyadic Cantor fractal. The scattering properties of electrons in these superlattices may be modeled by using that of quantum particles in piecewise constant potential wells. The twist plots representing the reflection coefficient as function of the lacunarity parameter show the appearance of black curves with perfectly transparent tunnelling which may be classified as vertical, arc, and striation nulls. Approximate analytical formulae for these reflection-less curves are derived using the transfer matrix method. Comparison with the numerical results show their good accuracy.Comment: 12 pages, 3 figure

Relación entre el número de rivales y el precio: El caso de los servicios notariales

El documento presenta una propuesta de modelización de la interacción estratégica en el mercado de servicios notariales en el Perú y propone un ejercicio empírico, con la finalidad de evaluar hipótesis alternativas acerca de los efectos de variables estructurales sobre el precio de los servicios notariales

Dinámica de mercado en el largo plazo: El caso de la industria de Cervezas en el Perú

El documento presenta la evaluación del nivel de competencia existente en el mercado peruano de cervezas empleando, para ello, el estadístico H desarrollado por Panzar y Rosse (1987). De acuerdo a los valores que tenga el estadístico H, es posible identificar si un mercado presenta características competitivas (H=1), de competencia monopolística (0<H<1) o monopolio (H<0). El objetivo del estudio es analizar si con la entrada de otros competidores en la industria peruana de cerveza, ocurrida durante la segunda parte de la década pasada, la configuración o dinámica de la competencia en ese sector cambió o no

Reduction of Petri net maintenance modeling complexity via Approximate Bayesian Computation

The accurate modeling of engineering systems and processes using Petri nets often results in complex graph representations that are computationally intensive, limiting the potential of this modeling tool in real life applications. This paper presents a methodology to properly define the optimal structure and properties of a reduced Petri net that mimic the output of a reference Petri net model. The methodology is based on Approximate Bayesian Computation to infer the plausible values of the model parameters of the reduced model in a rigorous probabilistic way. Also, the method provides a numerical measure of the level of approximation of the reduced model structure, thus allowing the selection of the optimal reduced structure among a set of potential candidates. The suitability of the proposed methodology is illustrated using a simple illustrative example and a system reliability engineering case study, showing satisfactory results. The results also show that the method allows flexible reduction of the structure of the complex Petri net model taken as reference, and provides numerical justification for the choice of the reduced model structure

A cross-sectoral review of the current and potential maintenance strategies for composite structures

The interest in the use of composite materials in thin-walled structures has grown over the last decades due to their well-known superior mechanical performance and reduced weight when compared with traditional materials. Notwithstanding, composite structures are susceptible to damage during manufacturing and to fatigue degradation during service, which grants inspection and maintenance strategies outstanding importance in the duty of mitigating premature failures and reducing whole life cycle costs. This paper aims to provide a cross-sectoral view of the current and potential maintenance strategies that are drawing the attention of the different industries and researchers by reviewing the current use and limitations of composites structures, the impact of maintenance in the whole-life cycle of the composite structures, the health and condition monitoring techniques applied, and the benefits and limitations of the currently used and potential maintenance strategies. Finally, the health and condition monitoring techniques and maintenance approaches used by the different industries are contrasted to identify trends and divergences and suggest research gaps and industrial opportunities

A Long-Chain Flavodoxin Protects Pseudomonas aeruginosa from Oxidative Stress and Host Bacterial Clearance

Long-chain flavodoxins, ubiquitous electron shuttles containing flavin mononucleotide (FMN) as prosthetic group, play an important protective role against reactive oxygen species (ROS) in various microorganisms. Pseudomonas aeruginosa is an opportunistic pathogen which frequently has to face ROS toxicity in the environment as well as within the host. We identified a single ORF, hereafter referred to as fldP (for flavodoxin from P. aeruginosa), displaying the highest similarity in length, sequence identity and predicted secondary structure with typical long-chain flavodoxins. The gene was cloned and expressed in Escherichia coli. The recombinant product (FldP) could bind FMN and exhibited flavodoxin activity in vitro. Expression of fldP in P. aeruginosa was induced by oxidative stress conditions through an OxyR-independent mechanism, and an fldP-null mutant accumulated higher intracellular ROS levels and exhibited decreased tolerance to H2O2 toxicity compared to wild-type siblings. The mutant phenotype could be complemented by expression of a cyanobacterial flavodoxin. Overexpression of FldP in a mutT-deficient P. aeruginosa strain decreased H2O2-induced cell death and the hypermutability caused by DNA oxidative damage. FldP contributed to the survival of P. aeruginosa within cultured mammalian macrophages and in infected Drosophila melanogaster, which led in turn to accelerated death of the flies. Interestingly, the fldP gene is present in some but not all P. aeruginosa strains, constituting a component of the P. aeruginosa accessory genome. It is located in a genomic island as part of a self-regulated polycistronic operon containing a suite of stress-associated genes. The collected results indicate that the fldP gene encodes a long-chain flavodoxin, which protects the cell from oxidative stress, thereby expanding the capabilities of P. aeruginosa to thrive in hostile environments.Fil: Moyano, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); ArgentinaFil: Tobares, Romina Alín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); ArgentinaFil: Rizzi, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); ArgentinaFil: Krapp, Adriana del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Mondotte, Juan Alberto. Instituto Pasteur; FranciaFil: Bocco, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Bioquímica Clínica E Inmunología; ArgentinaFil: Saleh, Maria Carla. Instituto Pasteur; FranciaFil: Carrillo, Nestor Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Smania, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentin

Molecular architecture of Streptococcus pneumoniae surface thioredoxin-fold lipoproteins crucial for extracellular oxidative stress resistance and maintenance of virulence.

19 pags, 8 figs, tabsThe respiratory pathogen Streptococcus pneumoniae has evolved efficient mechanisms to resist oxidative stress conditions and to displace other bacteria in the nasopharynx. Here we character ize at physiological, functional and structural levels two novel surface-exposed thioredoxin-family lipoproteins, Etrx1 and Etrx2. The impact of both Etrx proteins and their r edox partner methionine sulfoxide reductase SpMsrAB2 on pneumococcal pathogenesis was assessed in mouse virulence studies and phagocytosis assays. The results demonstrate that loss of function of either both Etrx proteins or SpMsrAB2 dramatically attenuated pneumococcal virulence in the acute mouse pneumonia model and that Etrx proteins compensate each other. The deficiency of Etrx proteins or SpMsrAB2 further enhanced bacterial uptake by macrophages, and accelerated pneumococcal killing by H2O2 or free methionine sulfoxides (MetSO). Moreover, the absence of both Etrx redox pathways provokes an accumulation of oxidized SpMsrAB2 in vivo. Taken together our results reveal insights into the role of two extracellular electron pathways required for reduction of SpMsrAB2 and surface-exposed MetSO. Identification of this system and its target proteins paves the w ay for the design of novel a ntimicrobialsThe authors thank the PXIII beamline at SLS and the ESRF beamline ID14‐1 for access to synchrotron radiation. We are also grateful to Kristine Sievert‐Giermann, Nadine Gotzmann and Melanie Skibbe (Department of Genetics, University of Greifswald, Germany) for technical assistance. This work was supported by grants from the Deutsche Forschungsgemeinschaft DFG HA3125/4‐2 (to S.H.), DFG AN746/3‐1 (to H.A.), BFU2011‐25326 and S2010/BMD‐2457 (to J.A.H.) and EU FP7 CAREPNEUMO Grant EU‐CP223111 from the European Union (to J.A.H. and S.H.

Monitoring the thermally induced transition from sp3-hybridized into sp2-hybridized carbons

The preparation of carbons for technical applications is typically based on a treatment of a precursor, which is transformed into the carbon phase with the desired structural properties. During such treatment the material passes through several different structural stages, for example, starting from precursor molecules via an amorphous phase into crystalline-like phases. While the structure of non-graphitic and graphitic carbon has been well studied, the transformation stages from molecular to amorphous and non-graphitic carbon are still not fully understood. Disordered carbon often contains a mixture of sp3-, sp2-and sp1-hybridized bonds, whose analysis is difficult to interpret. We systematically address this issue by studying the transformation of purely sp3-hybridized carbons, that is, nanodiamond and adamantane, into sp2-hybridized non-graphitic and graphitic carbon. The precursor materials are thermally treated at different temperatures and the transformation stages are monitored. We employ Raman spectroscopy, WAXS and TEM to characterize the structural changes. We correlate the intensities and positions of the Raman bands with the lateral crystallite size La estimated by WAXS analysis. The behavior of the D and G Raman bands characteristic for sp2-type material formed by transforming the sp3-hybridized precursors into non-graphitic and graphitic carbon agrees well with that observed using sp2-structured precursors