Protein Timing and its Effects on Muscular Hypertrophy and Strength in Individuals Engaged in Weight-Training

The purpose of this review was to determine whether past research provides conclusive evidence about the effects of type and timing of ingestion of specific sources of protein by those engaged in resistance weight training. Two essential, nutrition-related, tenets need to be followed by weightlifters to maximize muscle hypertrophy: the consumption of 1.2-2.0 g protein.kg -1 of body weight, and ≥44-50 kcal.kg-1 of body weight. Researchers have tested the effects of timing of protein supplement ingestion on various physical changes in weightlifters. In general, protein supplementation pre- and post-workout increases physical performance, training session recovery, lean body mass, muscle hypertrophy, and strength. Specific gains, differ however based on protein type and amounts. Studies on timing of consumption of milk have indicated that fat-free milk post-workout was effective in promoting increases in lean body mass, strength, muscle hypertrophy and decreases in body fat. The leucine content of a protein source has an impact on protein synthesis, and affects muscle hypertrophy. Consumption of 3–4 g of leucine is needed to promote maximum protein synthesis. An ideal supplement following resistance exercise should contain whey protein that provides at least 3 g of leucine per serving. A combination of a fast-acting carbohydrate source such as maltodextrin or glucose should be consumed with the protein source, as leucine cannot modulate protein synthesis as effectively without the presence of insulin. Such a supplement post-workout would be most effective in increasing muscle protein synthesis, resulting in greater muscle hypertrophy and strength. In contrast, the consumption of essential amino acids and dextrose appears to be most effective at evoking protein synthesis prior to rather than following resistance exercise. To further enhance muscle hypertrophy and strength, a resistance weight- training program of at least 10–12 weeks with compound movements for both upper and lower body exercises should be followed

The Association Between Serum Vitamin D Concentration and Nueromuscular Function in Patients with Crohn's Disease

Serum vitamin D concentrations are not typically assessed in patients with Crohn's disease, even though neuromuscular complaints are one of the most common complaints observed. Crohn's patients, especially with small bowel resections, are at risk for hypovitaminosis D and fatigue. Purpose: To determine if Crohn's disease patients have low serum 25(OH)D concentrations and to determine the association between 25(OH)D and measures of neuromuscular function, such as muscle strength, muscle fatigue, nerve function, and quality of health. Methods: Nineteen Crohn's patients (9 male and 10 female), with at least one small bowel resection were tested. Isometric muscle strength at 45� of knee extension and flexion, EMG fatigue rates of the rectus femoris (RF) and vastus lateralis (VL), and the total physical score (PCS) and total mental score (MCS) from the SF-36 were collected. Statistics: Pearson correlations were calculated to determine the association between vitamin D and the measures of neuromuscular function. Results: The mean 25(OH)D was 32ng/ml. The Crohn's patients tested were 43.16 + 10.26 years with an average of 1.79 resections and 17.79 years with the disease. The mean peak torque (Nm) for knee extension was 75.24 + 45.39, and 28.94 + 12.76 for flexion. The mean average peak torque (Nm) for knee extension was 55.91 + 35.55, and 20.96 + 9.80 for flexion. The mean fatigue rates (Hz/sec) were -0.07 + 0.05 for RF and -0.03 + 0.04 for VL. Peroneal nerve latency mean was 4.28 + 1.75 ms and the mean amplitude was 2.26 + 2.03 mV. No significant differences at the � = 0.05 level for muscle strength, fatigue rates, nerve function, and the MCS were found. A significant (p = 0.02) positive correlation existed for the PCS (r = 0.55) of the SF-36. Conclusions: The average vitamin D concentrations were sufficient and vitamin D was not correlated to muscle strength, fatigue, or nerve function. The serum vitamin D concentrations were found to explain 30% of the variability of the PCS of their quality of health. Further studies are required to identify the exact mechanisms of the decreased strength and fatigue experience by Crohn's patients

A new role for complement C3: regulation of antigen processing through an inhibitory activity.

International audienceIncreasing evidence underlines the involvement of complement component C3 in the establishment of acquired immunity which appears to play a complex role and to act at different levels. As antigen proteolysis by antigen presenting cells is a key event in the control of antigen presentation efficiency, and consequently in the quality of the immune response, we investigated whether C3 could modulate this step. Our results demonstrate for the first time that C3 can interfere with antigen proteolysis: (i) proteolysis of tetanus toxin (TT) by the lysosomal fraction from a human monocytic cell line (U937) is impaired in the presence of C3, (ii) this effect is C3-specific and involves the C3c fragment of the protein, (iii) C3c is effective even after disulfide disruption, but none of its three constitutive peptides is individually accountable for this inhibitory effect and (iv) the target-protease(s) exhibit(s) a serine-protease activity. The physiological relevance of our results is demonstrated by experiments showing a subcellular colocalisation of TT and C3 after their uptake by U937 and the reduction of TT proteolysis once internalised together with C3. These results highlight a novel role for C3 that broadens its capacity to modulate acquired immune response

A randomized trial and novel SPR technique identifies altered lipoprotein-LDL receptor binding as a mechanism underlying elevated LDL-cholesterol in APOE4s

At a population level APOE4 carriers (~25% Caucasians) are at higher risk of cardiovascular diseases. The penetrance of genotype is however variable and influenced by dietary fat composition, with the APOE4 allele associated with greater LDL-cholesterol elevation in response to saturated fatty acids (SFA). The etiology of this greater responsiveness is unknown. Here a novel surface plasmon resonance technique (SPR) is developed and used, along with hepatocyte (with the liver being the main organ modulating lipoprotein metabolism and plasma lipid levels) uptake studies to establish the impact of dietary fatty acid composition on, lipoprotein-LDL receptor (LDLR) binding, and hepatocyte uptake, according to APOE genotype status. In men prospectively recruited according to APOE genotype (APOE3/3 common genotype, or APOE3/E4), triglyceride-rich lipoproteins (TRLs) were isolated at fasting and 4-6 h following test meals rich in SFA, unsaturated fat and SFA with fish oil. In APOE4s a greater LDLR binding affinity of postprandial TRL after SFA, and lower LDL binding and hepatocyte internalization, provide mechanisms for the greater LDL-cholesterol raising effect. The SPR technique developed may be used for the future study of the impact of genotype, and physiological and behavioral variables on lipoprotein metabolism

Yersinia enterocolitica Serum Resistance Proteins YadA and Ail Bind the Complement Regulator C4b-Binding Protein

Many pathogens are equipped with factors providing resistance against the bactericidal action of complement. Yersinia enterocolitica, a Gram-negative enteric pathogen with invasive properties, efficiently resists the deleterious action of human complement. The major Y. enterocolitica serum resistance determinants include outer membrane proteins YadA and Ail. Lipopolysaccharide (LPS) O-antigen (O-ag) and outer core (OC) do not contribute directly to complement resistance. The aim of this study was to analyze a possible mechanism whereby Y. enterocolitica could inhibit the antibody-mediated classical pathway of complement activation. We show that Y. enterocolitica serotypes O:3, O:8, and O:9 bind C4b-binding protein (C4bp), an inhibitor of both the classical and lectin pathways of complement. To identify the C4bp receptors on Y. enterocolitica serotype O:3 surface, a set of mutants expressing YadA, Ail, O-ag, and OC in different combinations was tested for the ability to bind C4bp. The studies showed that both YadA and Ail acted as C4bp receptors. Ail-mediated C4bp binding, however, was blocked by the O-ag and OC, and could be observed only with mutants lacking these LPS structures. C4bp bound to Y. enterocolitica was functionally active and participated in the factor I-mediated degradation of C4b. These findings show that Y. enterocolitica uses two proteins, YadA and Ail, to bind C4bp. Binding of C4bp could help Y. enterocolitica to evade complement-mediated clearance in the human host

Vanillin derived a carbonate dialdehyde and a carbonate diol : novel platform monomers for sustainable polymers synthesis

Vanillin has been regarded as one of the important biomass-based platform chemicals for aromatic polymers synthesis. Herein, novel symmetric bis(4-formyl-2-methoxyphenyl)carbonate (BFMC) and bis(4-(hydroxymethyl)-2-methoxyphenyl)carbonate (BHMC) polymeric monomers have been synthesized in high yields using vanillin as a raw chemical, which have been submitted for polymer synthesis via well-established polymeric strategies. A new class of poly(carbonate ester)s oligomers with amide moieties in their side chain can be prepared by using the BFMC as one of monomers via the Passerini three compound reaction (3CR). A new class of poly(carbonate ester)s oligomers and poly(carbonate urethane)s can be prepared via reactions between BHMC with dicarboxylic acid chlorides and diisocyanates, respectively. Their structure have been confirmed by 1H NMR, 13C NMR and FTIR, and the gel permeation chromatograph (GPC) analysis shows that the Mn of poly(carbonate ester)s oligomers ranges from 3100 to 7900 with PDI between 1.31 and 1.65, and the Mn of poly(carbonate urethane)s ranges from 16 400 to 24 400 with PDI ranging from 1.36 to 2.17. The DSC analysis shows that the poly(carbonate ester)s oligomers have relative low Tg ranging from 37.4 to 74.1 °C, and the poly(carbonate urethane)s have Tg ranging from 97.3 to 138.3 °C, mainly correlating to the structure of dicarboxylic acid chlorides and diisocyanates used

Acid-catalyzed isomerization of the p-menthadienes

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RPE stats file.sav

 Purpose: The number of overweight or obese adults in the United States continues to increase. Many diseases are linked to obesity; consequently, there is great need for research to improve prevention and treatment of this condition. The purpose of this study was to compare OMNI ratings of perceived exertion (OMNI-RPE) among normal weight versus obese individuals across stages of an incremental aerobic fitness test. Secondary purposes included determining differences between groups for OMNI-muscle hurt (pain), heart rate (HR) and oxygen consumption (VO2). Methods: Women 28.52(9.69) years of age of normal weight (BMI n = 17) and obese status (BMI ≥ 30 kg/m2; n = 12) completed two days of testing, including a modified Balke treadmill test. A 2 x 3 mixed model ANOVA (group x stage) was used to detect differences between normal weight and obese groups across all variables. Results: The obese group reported higher OMNI-RPE (p =.002), higher HR (p = 0.003) and significantly lower VO2 (p =.027) than the normal weight group for all stages of the Modified Balke. No group x stage interactions were found for any variables with the exception of muscle pain (p Conclusion: OMNI-RPE, pain, HR, and VO2 data suggest that obese women have different physiological and perceptual responses than normal weight women at the same aerobic exercise intensity. Obese women perceive exercise as harder and more painful than normal weight women during an incremental treadmill test, which suggests the need for modified exercise recommendations for obese women.  </p