Peranan Modal Sosial Bagi Petani Miskin untuk Mempertahankan Kelangsungan Hidup Rumah Tangga di Pedesaan Ngawi (Studi Kasus di Desa Randusongo Kecamatan Gerih Kabupaten Ngawi Provinsi Jawa Timur)

This study was a descriptive qualitative research aiming to describe the role of Social Capital for the poor farmer to sustain their household life. The subject of research consisted of poor farmers having a less-than-0.13 ha land conducted in RT 4 RW 6 Bulu II Hamlet, Randusongo Village, Gerih Subdistrict, Ngawi Regency, East Java Province.This study was conducted using in-depth interview, observation, and library study techniques. Meanwhile, the sampling technique used was maximum variation sampling taken seven informants as the sample, consisting of four informants: poor old, very poor old, poor young, and very poor young farmers, as the case informant, while the key informant of research consisted of: elders of Randusongo Village, Carik/Secretary of Randusongo Village, and Head of Bulu II Hamlet. Key informant was also used as the instrument of validating the field data from the informant.The case informants were selected based on the criterion specified by Statistical Central Agency (BPS), that was, those belonging to poor family, while key informants were the village elders considered as knowledgeable and understanding about the daily condition of poor farmers because they domiciled in the same hamlet, and the village apparatus considered as knowing the poor farmers' condition because they often gave them service regarding Bantuan Langsung Tunai ((BLT= Cash Direct Grant) now called Bantuan Langsung Sementara Masyarakat (BLSM = Temporary Public Grant), Raskin (rice for poor people) and other services.The result showed that bonding social capital played an important role for the poor farmers in sustaining their household life. It could be seen from those helping their poor close relatives in meeting their daily life needs. Bridging social capital is the strongest out of the three social capitals existing because many poor farmer household could survive because of their close neighbor's help. Linking social capital did not play a role at al because such organization as PKK (Pendidikan Kesejahteraan Keluarga = Family Welfare Education) or organization at either RT or RW level in Randusongo village did not work thereby could not contribute to reinforcing social capital within the society

Peranan Modal Sosial Bagi Petani Miskin untuk Mempertahankan Kelangsungan Hidup Rumah Tangga di Pedesaan Ngawi (Studi Kasus di Desa Randusongo Kecamatan Gerih Kabupaten Ngawi Provinsi Jawa Timur)

This study was a descriptive qualitative research aiming to describe the role of Social Capital for the poor farmer to sustain their household life. The subject of research consisted of poor farmers having a less-than-0.13 ha land conducted in RT 4 RW 6 Bulu II Hamlet, Randusongo Village, Gerih Subdistrict, Ngawi Regency, East Java Province.This study was conducted using in-depth interview, observation, and library study techniques. Meanwhile, the sampling technique used was maximum variation sampling taken seven informants as the sample, consisting of four informants: poor old, very poor old, poor young, and very poor young farmers, as the case informant, while the key informant of research consisted of: elders of Randusongo Village, Carik/Secretary of Randusongo Village, and Head of Bulu II Hamlet. Key informant was also used as the instrument of validating the field data from the informant.The case informants were selected based on the criterion specified by Statistical Central Agency (BPS), that was, those belonging to poor family, while key informants were the village elders considered as knowledgeable and understanding about the daily condition of poor farmers because they domiciled in the same hamlet, and the village apparatus considered as knowing the poor farmers\u27 condition because they often gave them service regarding Bantuan Langsung Tunai ((BLT= Cash Direct Grant) now called Bantuan Langsung Sementara Masyarakat (BLSM = Temporary Public Grant), Raskin (rice for poor people) and other services.The result showed that bonding social capital played an important role for the poor farmers in sustaining their household life. It could be seen from those helping their poor close relatives in meeting their daily life needs. Bridging social capital is the strongest out of the three social capitals existing because many poor farmer household could survive because of their close neighbor\u27s help. Linking social capital did not play a role at al because such organization as PKK (Pendidikan Kesejahteraan Keluarga = Family Welfare Education) or organization at either RT or RW level in Randusongo village did not work thereby could not contribute to reinforcing social capital within the society

Whole genome amplification: Use of advanced isothermal method

Laboratory method for amplifying genomic deoxyribonucleic acid (DNA) samples aiming to generate more amounts and sufficient quantity DNA for subsequent specific analysis is named whole genome amplification (WGA). This method is only way to increase input material from few cells and limited DNA contents. While PCR-based WGA methods have been under continuous development for over a decade, shortcomings of these methods enforced many researchers to switch to the use of non-PCR-based linear amplification techniques. Moreover, application of high fidelity and high possessive DNA polymerases enabled development of an isothermal WGA technique named multiple displacement amplification (MDA). MDA is not based on PCR and doses not require thermal cycling. It should be noted that, while MDA-based techniques proposed aiming to overcome the drawbacks of PCR-based methods however, MDA is still facing some challenges. It seems that PCR-based WGA methods alsohave some merits. One of the problems which encountered both MDA and PCR-based methods is in the amplification of degraded DNA templates. WGA methods such as T7-based linear amplification of DNA(TLAD), balanced-PCR amplification and restriction and circularization-aided rolling circle amplification (RCA-RCA) have been suggested to aim at amplification of such DNA templates.Keywords: Whole genome amplification, multiple displacement amplification (MDA), non PCR-based method

Effect of Common Medications on the Expression of SARS-CoV-2 Entry Receptors in Kidney Tissue

Besides the respiratory system, severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) infection was shown to affect other essential organs such as the kidneys. Early kidney involvement during the course of infection was associated with worse outcomes, which could be attributed to the direct SARS-CoV-2 infection of kidney cells. In this study, the effect of commonly used medications on the expression of SARS-CoV-2 receptor, angiotensin-converting enzyme (ACE)2, and TMPRSS2 protein in kidney tissues was evaluated. This was done by in silico analyses of publicly available transcriptomic databases of kidney tissues of rats treated with multiple doses of commonly used medications. Of 59 tested medications, 56% modified ACE2 expression, whereas 24% modified TMPRSS2 expression. ACE2 was increased with only a few of the tested medication groups, namely the renin-angiotensin inhibitors, such as enalapril, antibacterial agents, such as nitrofurantoin, and the proton pump inhibitor, omeprazole. The majority of the other medications decreased ACE2 expression to variable degrees with allopurinol and cisplatin causing the most noticeable downregulation. The expression level of TMPRSS2 was increased with a number of medications, such as diclofenac, furosemide, and dexamethasone, whereas other medications, such as allopurinol, suppressed the expression of this gene. The prolonged exposure to combinations of these medications could regulate the expression of ACE2 and TMPRSS2 in a way that may affect kidney susceptibility to SARS-CoV-2 infection. Data presented here suggest that we should be vigilant about the potential effects of commonly used medications on kidney tissue expression of ACE2 and TMPRSS2

Impact of alternative solid state forms and specific surface area of high-dose, hydrophilic active pharmaceutical ingredients on tabletability

YesIn order to investigate the effect of using different solid state forms and specific surface area (TBET) of active pharmaceutical ingredients on tabletability and dissolution performance, the mono- and dihydrated crystalline forms of chlorothiazide sodium and chlorothiazide potassium (CTZK) salts were compared to alternative anhydrous and amorphous forms, as well as to amorphous microparticles of chlorothiazide sodium and potassium which were produced by spray drying and had a large specific surface area. The tablet hardness and tensile strength, porosity, and specific surface area of single-component, convex tablets prepared at different compression pressures were characterized. Results confirmed the complexity of the compressibility mechanisms. In general it may be concluded that factors such as solid-state form (crystalline vs amorphous), type of hydration (presence of interstitial molecules of water, dehydrates), or specific surface area of the material have a direct impact on the tabletability of the powder. It was observed that, for powders of the same solid state form, those with a larger specific surface area compacted well, and better than powders of a lower surface area, even at relatively low compression pressures. Compacts prepared at lower compression pressures from high surface area porous microparticles presented the shortest times to dissolve, when compared with compacts made of equivalent materials, which had to be compressed at higher compression pressures in order to obtain satisfactory compacts. Therefore, materials composed of nanoparticulate microparticles (NPMPs) may be considered as suitable for direct compaction and possibly for inclusion in tablet formulations as bulking agents, APIs, carriers, or binders due to their good compactibility performanceSolid State Pharmaceutical Cluster (SSPC), supported by Science Foundation Ireland under Grant No. 07/SRC/B1158

Modeling Low Energy Demand Futures for Buildings: Current State and Research Needs

Buildings are key in supporting human activities and well-being by providing shelter and other important services to their users. Buildings are, however, also responsible for major energy use and greenhouse gas (GHG) emissions during their life cycle. Improving the quality of services provided by buildings while reaching low energy demand (LED) levels is crucial for climate and sustainability targets. Building sector models have become essential tools for decision support on strategies to reduce energy demand and GHG emissions. Yet current models have significant limitations in their ability to assess the transformations required for LED. We review building sector models ranging from the subnational to the global scale to identify best practices and critical gaps in representing transformations toward LED futures. We focus on three key dimensions of intervention (socio-behavioral, infrastructural, and technological), three megatrends (digitalization, sharing economy, and circular economy), and decent living standards. This review recommends the model developments needed to better assess LED transformations in buildings and support decision-making toward sustainability targets

Annex III: Scenarios and modelling methods

The use of scenarios and modelling methods are pillars in IPCC Working Group III (WGIII) Assessment Reports. Past WGIII assessment report cycles identified knowledge gaps about the integration of modelling across scales and disciplines, mainly between global integrated assessment modelling methods and bottom-up modelling insights of mitigation responses. The need to improve the transparency of model assumptions and enhance the communication of scenario results was also recognised. This annex on Scenarios and Modelling Methods aims to address some of these gaps by detailing the modelling frameworks applied in the WGIII Sixth Assessment Report (AR6) chapters and disclose scenario assumptions and its key parameters. It has been explicitly included in the Scoping Meeting Report of the WGIII contribution to the AR6 and approved by the IPCC Panel at the 46th Session of the Panel

Effects of Detraining on Resting Cerebral Blood Flow in Master Athletes

Please view abstract in the attached PDF file

Global, regional, and national prevalence and mortality burden of sickle cell disease, 2000-2021: a systematic analysis from the Global Burden of Disease Study 2021

BACKGROUND: Previous global analyses, with known underdiagnosis and single cause per death attribution systems, provide only a small insight into the suspected high population health effect of sickle cell disease. Completed as part of the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2021, this study delivers a comprehensive global assessment of prevalence of sickle cell disease and mortality burden by age and sex for 204 countries and territories from 2000 to 2021. METHODS: We estimated cause-specific sickle cell disease mortality using standardised GBD approaches, in which each death is assigned to a single underlying cause, to estimate mortality rates from the International Classification of Diseases (ICD)-coded vital registration, surveillance, and verbal autopsy data. In parallel, our goal was to estimate a more accurate account of sickle cell disease health burden using four types of epidemiological data on sickle cell disease: birth incidence, age-specific prevalence, with-condition mortality (total deaths), and excess mortality (excess deaths). Systematic reviews, supplemented with ICD-coded hospital discharge and insurance claims data, informed this modelling approach. We employed DisMod-MR 2.1 to triangulate between these measures-borrowing strength from predictive covariates and across age, time, and geography-and generated internally consistent estimates of incidence, prevalence, and mortality for three distinct genotypes of sickle cell disease: homozygous sickle cell disease and severe sickle cell β-thalassaemia, sickle-haemoglobin C disease, and mild sickle cell β-thalassaemia. Summing the three models yielded final estimates of incidence at birth, prevalence by age and sex, and total sickle cell disease mortality, the latter of which was compared directly against cause-specific mortality estimates to evaluate differences in mortality burden assessment and implications for the Sustainable Development Goals (SDGs). FINDINGS: Between 2000 and 2021, national incidence rates of sickle cell disease were relatively stable, but total births of babies with sickle cell disease increased globally by 13·7% (95% uncertainty interval 11·1-16·5), to 515 000 (425 000-614 000), primarily due to population growth in the Caribbean and western and central sub-Saharan Africa. The number of people living with sickle cell disease globally increased by 41·4% (38·3-44·9), from 5·46 million (4·62-6·45) in 2000 to 7·74 million (6·51-9·2) in 2021. We estimated 34 400 (25 000-45 200) cause-specific all-age deaths globally in 2021, but total sickle cell disease mortality burden was nearly 11-times higher at 376 000 (303 000-467 000). In children younger than 5 years, there were 81 100 (58 800-108 000) deaths, ranking total sickle cell disease mortality as 12th (compared to 40th for cause-specific sickle cell disease mortality) across all causes estimated by the GBD in 2021. INTERPRETATION: Our findings show a strikingly high contribution of sickle cell disease to all-cause mortality that is not apparent when each death is assigned to only a single cause. Sickle cell disease mortality burden is highest in children, especially in countries with the greatest under-5 mortality rates. Without comprehensive strategies to address morbidity and mortality associated with sickle cell disease, attainment of SDG 3.1, 3.2, and 3.4 is uncertain. Widespread data gaps and correspondingly high uncertainty in the estimates highlight the urgent need for routine and sustained surveillance efforts, further research to assess the contribution of conditions associated with sickle cell disease, and widespread deployment of evidence-based prevention and treatment for those with sickle cell disease. FUNDING: Bill & Melinda Gates Foundation