Review of Hosein Aabad Sugar Factory (HASF) Wastewater and Assessment of its Pollution Load

Sugar industry is one of the largest industries in the world. Hosein Abad sugar factory (HASF) is located in Hamadan province of Iran. The aim of this research was to evaluate the quality and quantity of wastewater of the said factory and to estimate the pollution load and its emission factor as prerequisite of the management and environmental pollutions control in the region. Random grab sampling is used in twelve days between October 2010 and March 2011 during peak hours of activity. In this research, nine quality parameters as the key variables are measured to evaluate the quality of wastewater. The obtained results indicate that, the amount of wastewater produced by HASF is about 2740 m3/day. Based on the said results, the average amount of five days Biochemical Oxygen Demand (BOD5) and Chemical Oxygen Demand (COD) in the raw wastewater was 1296 and 2328 mg/l respectively. The results of this research showed that the effluent wastewater of HASF in the present conditions is highly polluted due to inappropriate treating and is not appropriate for artificial recharge use, discharge in rivers and lakes or utilization in agriculture, according to the DoE standards

Fecundity reproductive cycle of a local population of Gammarus Pulex in Sepidan (Fars Province, Iran)

Abstract: Gammarus pulex reproductive was studied in Komehr spring, in Komehr village Fars province, South of Iran. Specimens were collected using a net with 1 mm mesh size. Water temperature, DO, PH, No2, No, Po4, Ec, K, Mg, Total hardens and TDS were measured. Biological parameters including length and wet weight of males and females, number of female having eggs, number of eggs presented in moraspum sac of female, sex ratio, egg size, fecundity and reproductive effort was studied. Mean length of both sex and mean wet weight of males increased in autumn, decreased in spring and increased again in summer. Number of eggs in marsopium sac, female's wet weight, fecundity, and reproductive effort increased from autumn toward summer and egg volume increased in autumn and winter and reduces a little with increasing in egg's number in spring and summer. All reproductive factors showed significant differences throughout the year

Identification and density determination of juvenile fishes in Khouzestan coastal waters

Aquatic animals possess specific biological characteristics and lifecycle. Without knowing about their biology and complete lifecycle, planning about their conservation cannot be effective. Following the previous studies on fish larvae in Khuzestan coastal waters this study was done to determination catch composition and density of juvenile fishes in Khuzestan coastal waters during 2007 to 2008. Studied area included two main fisheries ground, Lifeh-Busafe in west and Bahrekan in east coasts. Monthly random sampling was carried out by using shrimp trawl net with Akhtar vessel. Juvenile fish's means the fishes that completed their larval cycle and are look like their parents but have not been matured yet. The catch mean, biomass, CPUA and the stock size of different species were estimated by using Swept Area Method. Totally 101485 caught fishes included 38 families and 63 species. The most abundant trawled fishes were Thryssa vitriostris Leiognathus bindus Ilisha melastoma Penahia macrophthalmus and Johnius belangerii .These five species contain about 80 %, the species Arius dussumieri and Cynoglossus arel 4.5% and 3.2 % respectively and the others 12% of total caught fishes. In the east coasts the species L. bindus I. melastoma T. vitriostris P. macrophthalmus and in the west coasts the species P. macrophthalmus T. vitriostris J. belangerii and I.melastoma were the most abundant. Among 63 identified fish species , juvenile fishes of 23 species were observed which contain 26.4 % of total caught fishes .the fishes Arius dussumieri and Acantopagrus latus 100%, Liza subviridis, Leiognathus lineolatus and Triacantuhus biaculeatus more than 80 % and Pomadasys stridens Lagocephalus inermis Saurida tumbil and Thryssa vitrirostris more than 50 % were observed as juvenile stage. The most abundant juvenile fishes were T. vitrirostris L. bindus and Arius dussumieri respectively. The maximum (1852 kg/km^2) and the minimum (165 kg/km^2) values of CPUA were estimated in west coasts in July and December respectively. The maximum (2677 kg/km^2) and the minimum (153 kg/km^2) values of CPUA were estimated in east coasts in June and August respectively. The biomass showed a distinct peak in June and July in east and west coasts respectively. The highest diversity index in east (2/22) and west (2.36) coasts were obtained in July. In present study the number of identified fishes is more than previous studies. Juvenile fishes spend their sensitive period of lifecycle in Khuzestan coastal waters. After growing in late spring, fish larvae enter to the juvenile stage and select this productive area as nursery ground. Increasing of juvenile fishes in July is accordance with their reproduction season in studied area. The east coasts are deeper than the west and there are some differences in hydrological characteristics due to discharging of the rivers Bahmanshir and Arvandrood in west coasts. The peaks of abundance in east and west coasts were observed with a clear time interval. The higher CPUA in east coasts can be caused due to gradual migration of juvenile fishes from west to east during cold season

Public data and open source tools for multi-assay genomic investigation of disease

Molecular interrogation of a biological sample through DNA sequencing, RNA and microRNA profiling, proteomics and other assays, has the potential to provide a systems level approach to predicting treatment response and disease progression, and to developing precision therapies. Large publicly funded projects have generated extensive and freely available multi-assay data resources; however, bioinformatic and statistical methods for the analysis of such experiments are still nascent. We review multi-assay genomic data resources in the areas of clinical oncology, pharmacogenomics and other perturbation experiments, population genomics and regulatory genomics and other areas, and tools for data acquisition. Finally, we review bioinformatic tools that are explicitly geared toward integrative genomic data visualization and analysis. This review provides starting points for accessing publicly available data and tools to support development of needed integrative methods

Revisiting inconsistency in large pharmacogenomic studies

In 2013, we published a comparative analysis of mutation and gene expression profiles and drug sensitivity measurements for 15 drugs characterized in the 471 cancer cell lines screened in the Genomics of Drug Sensitivity in Cancer (GDSC) and Cancer Cell Line Encyclopedia (CCLE). While we found good concordance in gene expression profiles, there was substantial inconsistency in the drug responses reported by the GDSC and CCLE projects. We received extensive feedback on the comparisons that we performed. This feedback, along with the release of new data, prompted us to revisit our initial analysis. We present a new analysis using these expanded data, where we address the most significant suggestions for improvements on our published analysis - that targeted therapies and broad cytotoxic drugs should have been treated differently in assessing consistency, that consistency of both molecular profiles and drug sensitivity measurements should be compared across cell lines, and that the software analysis tools provided should have been easier to run, particularly as the GDSC and CCLE released additional data. Our re-analysis supports our previous finding that gene expression data are significantly more consistent than drug sensitivity measurements. Using new statistics to assess data consistency allowed identification of two broad effect drugs and three targeted drugs with moderate to good consistency in drug sensitivity data between GDSC and CCLE. For three other targeted drugs, there were not enough sensitive cell lines to assess the consistency of the pharmacological profiles. We found evidence of inconsistencies in pharmacological phenotypes for the remaining eight drugs. Overall, our findings suggest that the drug sensitivity data in GDSC and CCLE continue to present challenges for robust biomarker discovery. This re-analysis provides additional support for the argument that experimental standardization and validation of pharmacogenomic response will be necessary to advance the broad use of large pharmacogenomic screens

Study on agricultural waste and brackish waters in Ahvaz and Khuramshahr to development aquaculture

This study was done in 2008 on agricultural sewage for aquaculture in six stations. Two stations were in Ahvaz Khorramshahr road near sugar cane plantation sewage in west Karoon, Two stations were in Ahvaz-Abadan road in sugar cane plantation sewage in east Karoon and Azadegan sewage and two stations were in agricultural sewage in the north of Ahvaz. Monthly Samplings of planktons, physical, chemical parameters were taken during Aprile to March 2008. In addition seasonal sampling of benthic fauna and fish were carried out. Heavy metal and toxic samples were done only one time during the study period. Measurement of some parameters such as temperature and pH were done during sampling procedure and other parameters were measured in laboratory based on standard methods. Heavy metal concentrations were measured with polarography and voltammetry techniques and toxic substances were extracted and concentrated to determine the concentration of them in water using GC device. For identifying phytoplanktons, 1 liter water from middle depth was sampled in each station and 4% formalin was added. Zooplankton were sampled monthly using 55µ plankton net. Fish samplings were done using cast net regarding regional condition, and sampling from benthic invertebrate of river were done using Petersen grab with 15.5cm*15.5cm. Results of water quality index (WQI) from Koushk Talaieh (station1), Artificial lake station (station 2), Farabi(station 3), and Azadegan aquaculture sewage station (station4) showed that water quality is in group four. WQS index was used for Maleh(station 5) and Pumping station (6 station) stations due to high levels of salinity. The WQS results showed that water quality of these stations were moderate to low. Heavy metals in water, the ordination is as follows: Ni>Zn>Pb>Cu>Co>Cd>Hg Mercury levels in all cases were less than the standard and all the elements have been somewhat less than chronic toxicity level. The values of all studied metals are lower than the WHO limit. The results indicated that chlorinated pesticides in all samples are at low level and they are at acceptable levels for aquaculture. In this study, 34 genus of phytoplankton from Bacilariophyceae (diatoms), Cyanophyceae, Chlorophyceae and Dinophyceae were present with 11, 8, 14 and one genus respectively and the percentage of frequencies were respectively 37.64, 34.26, 23.24 and 4.87%. In general phytoplanktons of wastewater were in modest condition for using in aquaculture. In this study, about 1,000 samples of zooplankton in 6 stations were identified and counted during the year. The high percentage of zooplankton were belonged to three groups of protozoa (mainly ciliates), Rotifer and copepods were. The Rotifers with more than 90 percent frequency had the maximum prevalence. The dominant rotifer species was Brachionus calciferous. Although in this study the dominant group of zooplanktons was rotifers but the frequency is not high. Therefore we cannot call this circumstance as eutrophic condition, however the growing trend of high food phenomenon has announced. High benthic diversity was observed in artificial lake wastewater station and Azadegan aquaculture sewage station. The most frequent taxa were in Azadegan aquaculture sewage station during autumn and winter which belonged to resistant Chironemidae larvae. In Koushk Talaieh, Farabi and Maleh stations, Chironemidae was dominanted and in Pumping station Coleoptera was dominant group. Hilsenhoff index showed that most of waste water stations were in poor to very poor condition, indicating that they were heavily contaminated. The data analysis of macrobenthose data in wastewaters based on Satropi and biological value (Z) showed that potential of fish production in Azadegan and artificial lake was high during the year. These two water sources have suitable situation for potential production and biological value, therefore with special plans for culturing low demand fish would be possible. In this study, 7 fish species belongs to 7 genera from 4 families were collected. Freshwater and marine water species was observed in the catch composition. According to the results of the four studied stations, artificial lake station and Farabi had the highest number of catch which mostly belonged to Acanttopetrus Latus and Liza Abuo. In general, based on biological parameters, such as zooplankton frequency, all stations had similar conditions but in terms of phytoplankton, Azadegan aquaculture sewage station, Pumping stations and Farabi wastewater have better condition for aquaculture. The FBI index showed that the artificial lake station and pumping station are more suitable for aquaculture activity. Low demand fish is recommended for Azadegan aquaculture sewage station. Artificial lake station had lower primary production but it is more suitable for aquaculture because of its lower FBI index value. Most of the fish presence was in the artificial lake station and Farabi wastewater which belonged to two predominant species of Acanttopetrus Latus and Liza Abuo. In general, based on biological and non-biological parameters, artificial lake station in the west Karoon and Azadegan aquaculture sewage station have better water quality than the other stations for aquaculture activities. This requires management action and special schemes

Conservation and restoration of Caspian salmon (Salmo trutta caspius) stocks in the southern Caspian

The project is funded by the CASPECO Project which is the Third Phase of the GEF supported project in the Caspian Sea region titled “The Caspian Sea: Restoring Depleted Fisheries and Consolidation of a Permanent Regional Environmental Governance Framework”, and implemented by the Iranian Fisheries Research Organization (IFRO) in partnership with four Coastal Research Centers affiliated to IFRO, Coldwater Fishes Research Centre (CFRC).The project's objective is to improve the conservation and restoration of Caspian Salmon (Salmo trutta caspius) stocks. In accordance with IUCN criteria Caspian Salmon is placed in the red list of endangered fish. In the recent decades the destruction of habitats for juveniles, reduction of genetic diversity and fish immune system were main causes of the Caspian salmon decline. Numbers of public meetings were held with participation of fishing communities’ representatives, experts, relevant local authorities and experts of the IFRO and Iranian Fisheries Organization (IFO), Iran Environment Protection Organization (IEPO), Water Resource Management (Ministry of Energy) and University senior professors, in order to create interaction and understanding of the project objectives. Educational awareness and extension program were conducted with the involvement of fisheries communities, private sector and NGO associations. The results of the project suggests that cross-sectoral coordination for sustainable fisheries can be achieved through building partnerships and enhancing communication between the environment, private, fisheries sectors and academia. Cross-sectoral cooperation through this project improved public awareness on growing risks facing Caspian Salmon from different sources including local sources (illegal fishing and over-fishing, urban wastewater, environment destruction, industrial pollutants, pollution of air and water resources), new emerging and invasive organisms, climate change, implications of the Caspian salmon population decline on economic and social affairs of the people who depend on them for their livelihood and food security and their role in fish stock protection. The project, through its capacity building, extension and educational programs and efforts to link science to management, could translate scientific findings into an action agenda for managers and policy makers that can bring an improvement in the future of the Caspian Sea salmon stock

Study of tagging effects on surviving rate of Caspian trout (Salmo trutta caspius) fries before releasing to natural habitat

Study of tagging effects on survival rate of Caspian trout (Salmo trutta caspius) fries before releasing to natural habitat was a part of a large research plan on conservation and restoration of Caspian trout stocks in the Southern Caspian by Coldwater Fishes Research Center. Catching brooders, propagation, larvae and juvenile rearing were carried out in Southern coasts and rivers of Caspian sea and in shahid Bahonar propagation center in Kelardasht, Mazandaran (North of Iran) in autumn 2012. 150 fries for each treatment (5, 10 and 20 g fries and Plastic T tag, elastomer tag and control treatments) anesthetized, weighed (g), measured (mm), tagged, disinfected and introduced to cement ponds. Fries were kept and fed for 5 days and their behavior and responses to food, infection of wound and mortality were recorded. No mortality were observed in all treatments, fries were shocked for few hours after handling and did not take food but they started to feed from the first day after tagging and there was no infection in macroscopic observation of wound

Providing brood stocks of Caspian trout (Salmo trutta caspius) from Mazandaran Rivers and production of one hundred thousand fries

Caspian trout (Salmo trutta caspius) is one of valuable fishes of the Caspian sea for people living in the Northern coastal area of Iran. Since fish stock and natural resources protection is one the Iranian fisheries research objectives, the most activity on Caspian trout rehabilitation was focused on releasing thousands of smolts in the rivers discharge to Caspian Sea. Catch statistics of this species has declined over last decades thus this study on quality of provided brood stocks and producing fries with suitable quality can help rehabilitation and rearing of this valuable species. Several rivers were considered for providing brooders to keep genetic resources variety of sea stocks. In spawning season 25 (9 male, 16 female) and 30 (5 male, 25 female) brooders were collected from fishermen in Mazandaran rivers and Gyilan coasts provinces respectively. After propagation and larvae rearing, 11500 (10-15 g.) and 50000 (less than 10 g.) juveniles were released in Gyilan and Mazandaran rivers respectively

Consensus on Molecular Subtypes of High-grade Serous Ovarian Carcinoma

Purpose: The majority of ovarian carcinomas are of high-grade serous histology, which is associated with poor prognosis. Surgery and chemotherapy are the mainstay of treatment, and molecular characterization is necessary to lead the way to targeted therapeutic options. To this end, various computational methods for gene expression-based subtyping of high-grade serous ovarian carcinoma (HGSOC) have been proposed, but their overlap and robustness remain unknown. Experimental Design: We assess three major subtype classifiers by meta-analysis of publicly available expression data, and assess statistical criteria of subtype robustness and classifier concordance. We develop a consensus classifier that represents the subtype classifications of tumors based on the consensus of multiple methods, and outputs a confidence score. Using our compendium of expression data, we examine the possibility that a subset of tumors are unclassifiable based on currently proposed subtypes. Results: HGSOC subtyping classifiers exhibit moderate pairwise concordance across our data compendium (58.9%-70.9%, p \u3c 10-5) and are associated with overall survival in a metaanalysis across datasets (p \u3c 10-5). Current subtypes do not meet statistical criteria for robustness to re-clustering across multiple datasets (Prediction Strength \u3c 0.6). A new subtype classifier is trained on concordantly classified samples to yield a consensus classification of patient tumors that correlates with patient age, survival, tumor purity, and lymphocyte infiltration. Conclusion: A new consensus ovarian subtype classifier represents the consensus of methods, and demonstrates the importance of classification approaches for cancer that do not require all tumors to be assigned to a distinct subtype