Adaptive Optics Spectroscopy of the [Fe II] Outflows from HL Tauri and RW Aurigae

We present new results of [Fe II] 1.644-micron spectroscopy toward the jets from HL Tau and RW Aur carried out with the Subaru Telescope combined with the adaptive optics system. We observed the regions within 2" - 3" from the stars with the sub-arcsecond resolutions of 0."5 and 0."2 for HL Tau and RW Aur, respectively. In addition to the strong, high velocity emission extended along each jet, we detected a blueshifted low velocity emission feature seen as a wing or shoulder of the high velocity emission at each stellar position. Detailed analysis shows that the position-velocity diagrams (PVDs) of HL Tau and RW Aur show a characteristic similar to those of the cold disk wind and X-wind models in that the [Fe II] line width is broad in the vicinity of the stellar position and is narrower at the extended jet. A closer comparison suggests, however, that the disk wind model tends to have too large line width at the jet while the X-wind model has excess emission on the redshifted side at the stellar position. The narrow velocity width with symmetric line profiles of the observed high velocity emission supports an X-wind type model where the launching region is localized in a small radial range, while the low velocity emission located away from the star favors the presence of a disk wind. The [Fe II] emission from the HL Tau jet shows a gap of 0."8 between the redshifted jet and the star, indicating the presence of an optically thick disk of ~ 160 AU in radius. The [Fe II] emission from the RW Aur jet shows a marked drop from the redshifted peak at Y ~ -0."2 toward the star, suggesting that its disk radius is smaller than 40 AU.Comment: Accepted in the ApJ (October 2006, v649n2), AAS LaTEX macros v 5.2, Total 25 pages with 7 figure

High resolution imaging polarimetry of HL Tau and magnetic field structure

We present high quality near infrared imaging polarimetry of HL Tau at 0.4 to 0.6 arcsec resolution, obtained with Subaru/CIAO and UKIRT/IRCAM. 3-D Monte Carlo modelling with aligned oblate grains is used to probe the structure of the circumstellar envelope and the magnetic field, as well as the dust properties. At J band the source shows a centrosymmetric pattern dominated by scattered light. In the H and K bands the central source becomes visible and its polarisation appears to be dominated by dichroic extinction, with a position angle inclined by ~40 degrees to the disc axis. The polarisation pattern of the environs on scales up to 200 AU is consistent with the same dichroic extinction signature superimposed on the centrosymmetric scattering pattern. These data can be modelled with a magnetic field which is twisted on scales from tens to hundreds of AU, or alternatively by a field which is globally misaligned with the disc axis. A unique solution to the field structure will require spatially resolved circular polarisation data. The best fit Monte Carlo model indicates a shallow near infrared extinction law. When combined with the observed high polarisation and non-negligible albedo these constraints can be fitted with a grain model involving dirty water ice mantles in which the largest particles have radii slightly in excess of 1 um. The best fit model has an envelope structure which is slightly flattened on scales up to several hundred AU. Both lobes of the bipolar outflow cavity contain a substantial optical depth of dust (not just within the cavity walls). Curved, approximately parabolic, cavity walls fit the data better than a conical cavity. The small inner accretion disc observed at millimetre wavelengths is not seen at this spatial resolution.Comment: Accepted by MNRAS, 21 pages, 10 figure

The whole blood transcriptional regulation landscape in 465 COVID-19 infected samples from Japan COVID-19 Task Force

「コロナ制圧タスクフォース」COVID-19患者由来の血液細胞における遺伝子発現の網羅的解析 --重症度に応じた遺伝子発現の変化には、ヒトゲノム配列の個人差が影響する--. 京都大学プレスリリース. 2022-08-23.Coronavirus disease 2019 (COVID-19) is a recently-emerged infectious disease that has caused millions of deaths, where comprehensive understanding of disease mechanisms is still unestablished. In particular, studies of gene expression dynamics and regulation landscape in COVID-19 infected individuals are limited. Here, we report on a thorough analysis of whole blood RNA-seq data from 465 genotyped samples from the Japan COVID-19 Task Force, including 359 severe and 106 non-severe COVID-19 cases. We discover 1169 putative causal expression quantitative trait loci (eQTLs) including 34 possible colocalizations with biobank fine-mapping results of hematopoietic traits in a Japanese population, 1549 putative causal splice QTLs (sQTLs; e.g. two independent sQTLs at TOR1AIP1), as well as biologically interpretable trans-eQTL examples (e.g., REST and STING1), all fine-mapped at single variant resolution. We perform differential gene expression analysis to elucidate 198 genes with increased expression in severe COVID-19 cases and enriched for innate immune-related functions. Finally, we evaluate the limited but non-zero effect of COVID-19 phenotype on eQTL discovery, and highlight the presence of COVID-19 severity-interaction eQTLs (ieQTLs; e.g., CLEC4C and MYBL2). Our study provides a comprehensive catalog of whole blood regulatory variants in Japanese, as well as a reference for transcriptional landscapes in response to COVID-19 infection

DOCK2 is involved in the host genetics and biology of severe COVID-19

「コロナ制圧タスクフォース」COVID-19疾患感受性遺伝子DOCK2の重症化機序を解明 --アジア最大のバイオレポジトリーでCOVID-19の治療標的を発見--. 京都大学プレスリリース. 2022-08-10.Identifying the host genetic factors underlying severe COVID-19 is an emerging challenge. Here we conducted a genome-wide association study (GWAS) involving 2, 393 cases of COVID-19 in a cohort of Japanese individuals collected during the initial waves of the pandemic, with 3, 289 unaffected controls. We identified a variant on chromosome 5 at 5q35 (rs60200309-A), close to the dedicator of cytokinesis 2 gene (DOCK2), which was associated with severe COVID-19 in patients less than 65 years of age. This risk allele was prevalent in East Asian individuals but rare in Europeans, highlighting the value of genome-wide association studies in non-European populations. RNA-sequencing analysis of 473 bulk peripheral blood samples identified decreased expression of DOCK2 associated with the risk allele in these younger patients. DOCK2 expression was suppressed in patients with severe cases of COVID-19. Single-cell RNA-sequencing analysis (n = 61 individuals) identified cell-type-specific downregulation of DOCK2 and a COVID-19-specific decreasing effect of the risk allele on DOCK2 expression in non-classical monocytes. Immunohistochemistry of lung specimens from patients with severe COVID-19 pneumonia showed suppressed DOCK2 expression. Moreover, inhibition of DOCK2 function with CPYPP increased the severity of pneumonia in a Syrian hamster model of SARS-CoV-2 infection, characterized by weight loss, lung oedema, enhanced viral loads, impaired macrophage recruitment and dysregulated type I interferon responses. We conclude that DOCK2 has an important role in the host immune response to SARS-CoV-2 infection and the development of severe COVID-19, and could be further explored as a potential biomarker and/or therapeutic target

RELATIONSHIP BETWEEN SALT INTAKE AND GNRI IN ELDERLY DIALYSIS PATIENTS

While the recommended salt intake in dialysis patients is no more than 5g/day in the KDOQI guideline, and 6g/day in the JSH 2009 guideline, reducing salt consumption is difficult on the traditional Japanese diet. If a patient is malnourished, a low-salt diet poses a risk of aggravating the nutritional deficiency. Since elderly dialysis patients have nutritional deficiencies underlying their condition, the recommended low-salt diet may prevent these patients from receiving adequate nutrition. In the present study, factors associated with nutritional status in the elderly were assessed using the Geriatric Nutritional Risk Index (GNRI), which is considered to correlate with predictor of mortality among dialysis patients. Participating patients were anuric, had been maintained on dialysis for at least 2 years, and were 65 years of age or older. Factors assessed for their possible correlations with GNRI were primary disease, presence of spouse, presence of cohabiting family, weight gain, and estimated salt intake. We analyzed 36 patients (age 74.3±5.4 years, 50% males). GNRI was 90.9±7.7, and salt intake (8.02±1.94) correlated with GNRI (r=0.41, P=0.02). No correlations were detected for the presence of spouse or cohabiting family, which would have contributed to nutrition. In conclusion, the higher the salt intake, the better GNRI tended to be. This raised the possibility that it would be advantageous to avoid excessive salt restriction in nutritional training