Prevention of Protein Glycation by Natural Compounds

Non-enzymatic protein glycosylation (glycation) contributes to many diseases and aging of organisms. It can be expected that inhibition of glycation may prolong the lifespan. The search for inhibitors of glycation, mainly using in vitro models, has identified natural compounds able to prevent glycation, especially polyphenols and other natural antioxidants. Extrapolation of results of in vitro studies on the in vivo situation is not straightforward due to differences in the conditions and mechanism of glycation, and bioavailability problems. Nevertheless, available data allow to postulate that enrichment of diet in natural anti-glycating agents may attenuate glycation and, in consequence, ageing

Effect of Antioxidants Supplementation on Aging and Longevity

If aging is due to or contributed by free radical reactions, as postulated by the free radical theory of aging, lifespan of organisms should be extended by administration of exogenous antioxidants. This paper reviews data on model organisms concerning the effects of exogenous antioxidants (antioxidant vitamins, lipoic acid, coenzyme Q, melatonin, resveratrol, curcumin, other polyphenols, and synthetic antioxidants including antioxidant nanoparticles) on the lifespan of model organisms. Mechanisms of effects of antioxidants, often due to indirect antioxidant action or to action not related to the antioxidant properties of the compounds administered, are discussed. The legitimacy of antioxidant supplementation in human is considered

Kinetics of Glycoxidation of Bovine Serum Albumin by Methylglyoxal and Glyoxal and its Prevention by Various Compounds

The aim of this study was to compare several methods for measurement of bovine serum albumin (BSA) modification by glycoxidation with reactive dicarbonyl compounds (methylglyoxal ‒ MGO and glyoxal ‒ GO), for studies of the kinetics of this process and to compare the effects of 19 selected compounds on BSA glycation by the aldehydes. The results confirm the higher reactivity of MGO with respect to GO and point to the usefulness of AGE, dityrosine and N′-formylkynurenine fluorescence for monitoring glycation and evaluation of protection against glycation. Different extent of protection against glycation induced by MGO and GO was found for many compounds, probably reflecting effects on various stages of the glycation process. Polyphenols (genistein, naringin and ellagic acid) were found to protect against aldehyde-induced glycation; 1-cyano-4-hydroxycinnamic acid was also an effective protector.The study has been supported by Grant 2011/01/M/NZ3/02065 from the Polish National Science Center. We are indebted to J. Skolimowski for the synthesis of nitroxide

Kinetics of Glycoxidation of Bovine Serum Albumin by Glucose, Fructose and Ribose and Its Prevention by Food Components

The aim of this study was to compare the kinetics of the glycoxidation of bovine serum albumin (BSA) as a model protein by three sugars: glucose, fructose and ribose, using fluorometric measurements of the content of advanced glycation end products (AGEs), protein-bound fructosamine, dityrosine, N'-formylkynurenine, kynurenine, tryptophan, the content of advanced oxidation protein products (AOPP), protein carbonyl groups, as well as thiol groups. Moreover, the levels of glycoalbumin and AGEs were determined by using an enzyme-linked immunosorbent assay. Based on the kinetic results, the optimal incubation time for studies of the modification of the glycoxidation rate by additives was chosen, and the effects of 25 compounds of natural origin on the glycoxidation of BSA induced by various sugars were examined. The same compounds were found to have different effects on glycoxidation induced by various sugars, which suggests caution in extrapolation from experiments based on one sugar to other sugars. From among the compounds tested, the most effective inhibitors of glycoxidation were: polyphenols, pyridoxine and 1-cyano-4- hydroxycinnamic acid.The study has been supported by Grant 2011/01/M/NZ3/02065 from the Polish National Science Center and performed within the COST (European Cooperation in Science and Technology) action CM1001

Oxidative Modification of Proteins in Pediatric Cystic Fibrosis with Bacterial Infections

Pseudomonas aeruginosa and Staphylococcus aureus cause chronic lung infection in cystic fibrosis (CF) patients, inducing chronic oxidative stress. Several markers of plasma protein oxidative damage and glycoxidation and activities of erythrocyte antioxidant enzymes have been compared in stable CF patients chronically infected with Pseudomonas aeruginosa ( = 12) and Staphylococcus aureus ( = 10) in relation to healthy subjects ( = 11). Concentration of nitric oxide was also measured in the exhaled air from the lower respiratory tract of patients with CF. Elevated glycophore (4.22 ± 0.91 and 4.19 ± 1.04 versus control 3.18 ± 0.53 fluorescence units (FU)/mg protein; < 0.05) and carbonyl group levels (1.9 ± 0.64, 1.87 ± 0.45 versus control 0.94 ± 0.19 nmol/mg protein; < 0.05) as well as increased glutathione S-transferase activity (2.51 ± 0.88 and 2.57 ± 0.79 U/g Hb versus 0.77 ± 0.16 U/g Hb; < 0.05) were noted in Pseudomonas aeruginosa and Staphylococcus aureus infected CF. Kynurenine level (4.91 ± 1.22 versus 3.89 ± 0.54 FU/mg protein; < 0.05) was elevated only in Staphylococcus aureus infected CF. These results confirm oxidative stress in CF and demonstrate the usefulness of the glycophore level and protein carbonyl groups as markers of oxidative modifications of plasma proteins in this diseaseThe study has been supported by the Polish Ministry of Science and Higher Education with an Iuventus Plus Grant IP201104797

Comparison of Antioxidants: The Limited Correlation between Various Assays of Antioxidant Activity

The inhibitory effects a range of synthetic and natural antioxidants on lipid peroxidation of egg yolk and erythrocyte membranes induced by a free radical generator 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH) was compared, with significant differences being found between both systems. When the protection by selected antioxidants against the effects of AAPH on erythrocytes (hemolysis, oxidation of hemoglobin and glutathione (GSH) and generation of reactive oxygen species (ROS)) was studied, most antioxidants were protective, but in some tests (oxidation of hemoglobin and GSH) some acted as prooxidants, inducing oxidation in the absence of AAPH and enhancing the AAPH-induced oxidation. These results demonstrate a diversified action of antioxidants in different systems and point to a need for careful extrapolation of any conclusions drawn from one parameter or experimental system to another.This study was performed within the project of the Minister of Science and Higher Education “Regional Initiative of Excellence”, Project No. 026/RID/2018/19

Rehabilitacja neurologiczna pacjentki po udarze mózgu – opis przypadku = Neurological rehabilitation of a patient after stroke – case report

Ratuszek-Sadowska Dorota, Kowalski Mikołaj, Woźniak Kamila, Kochański Bartosz, Hagner Wojciech. Rehabilitacja neurologiczna pacjentki po udarze mózgu – opis przypadku = Neurological rehabilitation of a patient after stroke – case report. Journal of Education, Health and Sport. 2016;6(8):28-40. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.59685http://ojs.ukw.edu.pl/index.php/johs/article/view/3725   The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).755 Journal of Education, Health and Sport eISSN 2391-8306 7© The Author (s) 2016;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 02.07.2016. Revised 25.07.2016. Accepted: 28.07.2016.  Rehabilitacja neurologiczna pacjentki po udarze mózgu – opis przypadku Neurological rehabilitation of a patient after stroke – case report Dorota Ratuszek-Sadowska1, Mikołaj Kowalski1, Kamila Woźniak2, Bartosz Kochański1, Wojciech Hagner11.         Katedra i Klinika Rehabilitacji, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu2.         Klinika Neurochirurgii, Neurotraumatologii i Neurochirurgii Dziecięcej, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu  Streszczenie Udary mózgu stanowią najczęstszą przyczynę trwałej niepełnosprawności, prowadząc do utraty zdolności aktywnego życia. Terapia neurologiczna pacjentów po przebytym udarze jest głównie ukierunkowana na największy możliwy wzrost funkcji. Podczas terapii stosowane są różne techniki mające na celu hamowanie patologicznych wzorców ruchowych, a także stymulację reakcji o charakterze fizjologicznym.Celem niniejszej pracy jest omówienie, na podstawie zaprezentowanego przypadku, postępowania rehabilitacyjnego u 29-letniej pacjentki po udarze niedokrwiennym prawej półkuli mózgu w przednim obszarze unaczynienia.Badanie fizjoterapeutyczne, opierające się na Międzynarodowej Klasyfikacji ICF, oceniające zaburzenia w obrębie struktur ciała, funkcji oraz aktywności i uczestnictwa, wykazało osłabienie mięśni dolnego tułowia, dna miednicy oraz lewego biodra, hipotonię mięśniową strony bezpośrednio zajętej, a także zwiększone napięcie mięśniowe dystalnie w lewej kończynie górnej. Problemami pacjentki na poziomie aktywności było samodzielne wstawanie i stanie bez asekuracji oraz brak aktywności kończyny górnej lewej.Prowadzona terapia obejmowała regulacje napięcia mięśniowego, pracę nad wzmocnieniem mięśni tułowia lewego biodra oraz miednicy, torowanie prawidłowego obciążania strony bezpośrednio zajętej podczas zmian pozycji z siadu do stania oraz chodu, a także naukę czynności dnia codziennego. Słowa kluczowe: udar mózgu, rehabilitacja, terapia neurologiczna, ICF. AbstractStroke is the most common cause of permanent disability, leading to loss of active life. Neurological therapy of the patients after stroke is mainly aimed at the greatest possible growth of the function. During the treatment there are various techniques designed to inhibit pathological movement patterns, as well as the stimulation of a physiological response.The aim of this paper is to discuss, on the basis of the presented case, rehabilitation methods of 29-year-old patient after ischemic stroke of the right hemisphere of the brain in the front area of the vasculature.The physiotherapy examination, based on the International Classification of ICF, assessing problems in the body structures, functions and activities and participation, showed a weakness of the lower trunk muscles, pelvic floor and left hip, muscles hypotonia of directly occupied side, as well as increased muscle tension in left upper limb. Problems of patient activity levels were independent standing up and able to stand without help and lack of left upper limb function activity.Neurological therapy included the muscle tone normalization, work on strengthen core, left hip and pelvis muscles, priming the correct loading of the directly occupied side during the position changes from sitting position to standing and walking, as well as learning of daily living activities. Key words: stroke, rehabilitation, neurological therapy, ICF

Molecular strategies to prevent, inhibit and degrade advanced glycoxidation and advanced lipoxidation end products

The advanced glycoxidation end products (AGEs) and lipoxidation end products (ALEs) contribute to the development of diabetic complications and of other pathologies. The review discusses the possibilities of counteracting the formation and stimulating the degradation of these species by pharmaceuticals and natural compounds. The review discusses inhibitors of ALE and AGE formation, cross-link breakers, ALE/AGE elimination by enzymes and proteolytic systems, receptors for advanced glycation end products (RAGEs) and blockade of the ligand-RAGE axis

Identification of a biomarker panel for improvement of prostate cancer diagnosis by volatile metabolic profiling of urine

Background: The lack of sensitive and specific biomarkers for the early detection of prostate cancer (PCa) is a major hurdle to improve patient management. Methods: A metabolomics approach based on GC-MS was used to investigate the performance of volatile organic compounds (VOCs) in general and, more specifically, volatile carbonyl compounds (VCCs) present in urine as potential markers for PCa detection. Results: Results showed that PCa patients (n = 40) can be differentiated from cancer-free subjects (n = 42) based on their urinary volatile profile in both VOCs and VCCs models, unveiling significant differences in the levels of several metabolites. The models constructed were further validated using an external validation set (n = 18 PCa and n = 18 controls) to evaluate sensitivity, specificity and accuracy of the urinary volatile profile to discriminate PCa from controls. The VOCs model disclosed 78% sensitivity, 94% specificity and 86% accuracy, whereas the VCCs model achieved the same sensitivity, a specificity of 100% and an accuracy of 89%. Our findings unveil a panel of 6 volatile compounds significantly altered in PCa patients' urine samples that was able to identify PCa, with a sensitivity of 89%, specificity of 83%, and accuracy of 86%. Conclusions: It is disclosed a biomarker panel with potential to be used as a non-invasive diagnostic tool for PCa.info:eu-repo/semantics/publishedVersio