Comparação de metodologia analítica na determinação de fósforo em solos

TCC (graduação) - Universidade Federal de Santa Catarina. Centro de Ciências Físicas e Matemáticas. Curso de Química.Amostras de solos foram analisadas em relação ao teor de fósforo empregando os métodos de extração Mehlich-1 utilizado rotineiramente na CIDASC e o método Mehlich-3. Os teores de fósforo extraídos empregando nove amostras de solos pelos dois métodos não apresentaram diferença significativa quando foi aplicado o teste t para múltiplas amostras para um nível de confiança de 95%

Redistricting: Drawing the Line

We develop methods to evaluate whether a political districting accurately represents the will of the people. To explore and showcase our ideas, we concentrate on the congressional districts for the U.S. House of representatives and use the state of North Carolina and its redistrictings since the 2010 census. Using a Monte Carlo algorithm, we randomly generate over 24,000 redistrictings that are non-partisan and adhere to criteria from proposed legislation. Applying historical voting data to these random redistrictings, we find that the number of democratic and republican representatives elected varies drastically depending on how districts are drawn. Some results are more common, and we gain a clear range of expected election outcomes. Using the statistics of our generated redistrictings, we critique the particular congressional districtings used in the 2012 and 2016 NC elections as well as a districting proposed by a bipartisan redistricting commission. We find that the 2012 and 2016 districtings are highly atypical and not representative of the will of the people. On the other hand, our results indicate that a plan produced by a bipartisan panel of retired judges is highly typical and representative. Since our analyses are based on an ensemble of reasonable redistrictings of North Carolina, they provide a baseline for a given election which incorporates the geometry of the state's population distribution.Comment: Corrected typos from previous version; added new plots showing stability; corrected error in EG plots and analysi

Conceptos de agroecología y marco analítico

Para analizar las políticas de agroecología y de producción orgánica en América Latina y el Caribe (ALC) nos preocupamos primero de caracterizar los antecedentes de la agroecología en la literatura, en particular al nivel regional. Luego, presentamos el marco analítico que movilizamos y la metodología para analizar las políticas e instrumentos en cada país y producir un análisis transversal. (Résumé d'auteur

Topics in cancer genomics

Large-scale projects such as the The Cancer Genome Atlas (TCGA) have generated extensive exome libraries across several disease types and populations. Detection of somatic changes in HLA genes by whole-exome sequencing (WES) has been complicated by the highly polymorphic nature of these loci. We developed a method POLYSOLVER (POLYmorphic loci reSOLVER) for accurate inference of class I HLA-A, -B and -C alleles from WES data, and achieved 97% accuracy at protein level resolution when this was applied to 133 HapMap samples of known HLA type. By applying POLYSOLVER in conjunction with somatic change detection tools to 2688 tumor/normal pairs TCGA that were previously analyzed by conventional approaches, we re-discovered 37 of 56 (66%) HLA mutations, while further identifying 23 new events. An analysis of WES data from a larger set of 3768 tumor/normal pairs by POLYSOLVER revealed 131 class I mutations with an enrichment for potentially loss-of-function events. 3% of samples had at least one HLA event with 95 of 131 mutations in the T cell interacting and peptide binding domains. Recurrent hotspot sites of missense, nonsense and splice site mutations were discovered that suggest positive selection, and support immune evasion as an important pathway in cancer. Exome sequencing has also revealed a large number of shared and personal somatic mutations across human cancers. In principle, any genetic alteration affecting a protein-coding region has the potential to generate mutated peptides that are presented by surface HLA class I proteins that might be recognized by cytotoxic T cells. Utilizing POLYSOLVER in conjunction with knowledge of mutations in other genetic loci inferred from exome data, we developed a pipeline for the prediction and validation of such neoantigens derived from individual tumors and presented by patient-specific alleles of the HLA proteins. We applied our computational pipeline to 91 chronic lymphocytic leukemias (CLL) that had undergone whole-exome sequencing. We predicted ~22 mutated HLA-binding peptides per leukemia (derived from ~16 missense mutations), and experimentally confirmed HLA binding for ~55% of such peptides. Finally, we computationally predicted HLA-binding peptides with missense or frameshift mutations for several cancer types and predicted dozens to thousands of neoantigens per individual tumor, suggesting that neoantigens are frequent in most tumors. The neoantigen prediction pipeline can also elucidate the neoantigens unique to a particular cancer patient and help in the design of personalized immune vaccines. MicroRNAs (miRs) are a class of non-coding small RNAs that regulate gene expression by promoting mRNA degradation or by inhibiting mRNA translation. Context Likelihood of Relatedness (CLR) is genetic network reconstruction method that considers the local network context in assessing the significance of connections while also allowing for detection of non-linear associations. Leveraging TCGA multidimensional data in glioblastoma, we inferred the putative regulatory network between microRNA and mRNA using the CLR algorithm. Interrogation of the network in context of defined molecular subtypes identified 8 microRNAs with a strong discriminatory potential between proneural and mesenchymal subtypes. Integrative in silico analyses, a functional genetic screen, and experimental validation identified miR-34a as a tumor suppressor in proneural subtype glioblastoma. Mechanistically, in addition to its direct regulation of platelet-derived growth factor receptor-alpha (PDGFRA), promoter enrichment analysis of CLR-inferred mRNA nodes established miR-34a as a novel regulator of a SMAD4 transcriptional network. Clinically, miR-34a expression level is shown to be prognostic, where miR-34a low-expressing glioblastomas exhibited better overall survival. This work illustrates the potential of comprehensive multidimensional cancer genomic data combined with computational and experimental models to enable mechanistic exploration of relationships among different genetic elements across the genome space in cancer

Le lobbying en France: une approche par le concept de corégulation

Unlike Anglo-Saxon countries, France has adopted only very recently (Sapin 2 Law, 2016) its official definition and a legal framework about corporate lobbying activities. Using the concept of coregulation, this article will focus on analyzing the link (or the interaction) between voluntary and binding standards which regulate lobbying. It will also identify public and private actors who initiated these standards. Finally, the limits of this new type of lobbying regulations will be underlined by studying them in a broader perspective on the CSR stakes (or challenges) of lobbying and on the paradoxes of the recent concept of responsible lobbying