Emigración y retención de Palinurus elephas (Fabricius, 1787) en un área marina protegida del Mediterráneo central occidental

This study describes the results obtained by applying the Arnason Schwartz multistate mark-recapture model to eight years of data collected in and around a small no-fishing marine protected area (MPA; 4 km2) in the central western Mediterranean. From 1997 to 2004, a total of 4044 specimens of Palinurus elephas (Fabr., 1787) were tagged and 317 recaptured. The most parsimonious model which best explained the data variability was that of a temporally constant rate of apparent survival and movement in each of the two strata. The absence of any temporal influence in the apparent survival rate inside the no-take area suggested that spillover and mortality are constant for each period of the study. The lower apparent survival rate in surrounding zones than inside the MPA (0.26 ± 0.04 (SE) vs 0.94 ± 0.03 (SE)) is presumed to be a function of fishing effort. A continuous movement of P. elephas across the boundary of the small MPA was also tested. This information on retention of lobsters in the MPA contributes to our understanding of the effect of introducing MPAs into a managed commercial fishery system.Este estudio presenta los resultados de aplicar el modelo multiestado de marcado y recaptura de Arnason Schwartz a una serie de ocho años de datos recolectados en el interior y alrededor de una pequeña área marina protegida (AMP) vedada a la pesca (4 km2) del Mediterráneo central occidental. Desde 1997 a 2004, un total de 4044 ejemplares de Palinurus elephas (Fabr., 1787) fueron marcados, de los cuales 317 fueron recapturados. El modelo más parsimonioso que explicó mejor la variabilidad de los datos fue aquel con una tasa temporal constante de aparente supervivencia y movimiento entre los dos estratos. La ausencia de influencia temporal sobre la tasa de supervivencia aparente en el interior del área protegida, sugirió que el “spillover” y la mortalidad son constantes para cada periodo del estudio. La menor tasa aparente de supervivencia en zonas alrededor de la reserva respecto al interior de la misma (0.26 ± 0.04 (SE) vs 0.94 ± 0.03 (SE)) se considera que es una función del esfuerzo pesquero. Un movimiento continuo de P. elephas a través de los límites de la pequeña AMP fue evaluado. Esta información sobre la retención de langostas en el AMP contribuye a comprender el efecto de la introducción de AMPs en un sistema regulado de pesquería comercial

Emigration and retention of <i>Palinurus elephas</i> (Fabricius, 1787) in a central western Mediterranean marine protected area

Este estudio presenta los resultados de aplicar el modelo multiestado de marcado y recaptura de Arnason Schwartz a una serie de ocho años de datos recolectados en el interior y alrededor de una pequeña área marina protegida (AMP) vedada a la pesca (4 km2) del Mediterráneo central occidental. Desde 1997 a 2004, un total de 4044 ejemplares de Palinurus elephas (Fabr., 1787) fueron marcados, de los cuales 317 fueron recapturados. El modelo más parsimonioso que explicó mejor la variabilidad de los datos fue aquel con una tasa temporal constante de aparente supervivencia y movimiento entre los dos estratos. La ausencia de influencia temporal sobre la tasa de supervivencia aparente en el interior del área protegida, sugirió que el "spillover" y la mortalidad son constantes para cada periodo del estudio. La menor tasa aparente de supervivencia en zonas alrededor de la reserva respecto al interior de la misma (0.26 ± 0.04 (SE) vs 0.94 ± 0.03 (SE)) se considera que es una función del esfuerzo pesquero. Un movimiento continuo de P. elephas a través de los límites de la pequeña AMP fue evaluado. Esta información sobre la retención de langostas en el AMP contribuye a comprender el efecto de la introducción de AMPs en un sistema regulado de pesquería comercial

Notch signaling sustains the expression of Mcl-1 and the activity of eIF4E to promote cell survival in CLL

In chronic lymphocytic leukemia (CLL), Notch1 and Notch2 signaling is constitutively activated and contributes to apoptosis resistance. We show that genetic inhibition of either Notch1 or Notch2, through small-interfering RNA, increases apoptosis of CLL cells and is associated with decreased levels of the anti-apoptotic protein Mcl-1. Thus, Notch signaling promotes CLL cell survival at least in part by sustaining Mcl-1 expression. In CLL cells, an enhanced Notch activation also contributes to the increase in Mcl-1 expression and cell survival induced by IL-4.Mcl-1 downregulation by Notch targeting is not due to reduced transcription or degradation by caspases, but in part, to increased degradation by the proteasome. Mcl-1 downregulation by Notch targeting is also accompanied by reduced phosphorylation of eukaryotic translation initiation factor 4E (eIF4E), suggesting that this protein is another target of Notch signaling in CLL cells.Overall, we show that Notch signaling sustains CLL cell survival by promoting Mcl-1 expression and eIF4E activity, and given the oncogenic role of these factors, we underscore the therapeutic potential of Notch inhibition in CLL

Movement patterns of the spiny lobster <em>Palinurus elephas</em> (Fabricius, 1787) from a central western Mediterranean protected area

Movement patterns of the spiny lobster Palinurus elephas were determined from 389 individuals (total tagged 5666) tag-recaptured inside a no-take area of the central western Mediterranean and its surrounding zone. High site association and limited movements in tagged lobsters was observed; 60.4% of lobsters moved less than 2 km from the centre of the area (site of release). No clear relationship between lobster movement pattern and sex or size was observed; however, it seemed that the largest males and females tended to be more resident, thus contributing to the rebuilding of the biomass of local lobsters. Most lobsters undertook migrations in the southwest direction. The increased availability of shelters and food towards the southwest could have contributed to the lobsters' movement. The results of our research indicate that the small size of the protected area and the scale of the movement exhibited by tagged lobsters allows a proportion of the lobster population to move out of the protected area and become susceptible to capture in the adjacent fishery

Pautas de movimiento de la langosta Palinurus elephas (Fabricius, 1787) desde un área protegida en el Mediterráneo occidental central

Movement patterns of the spiny lobster Palinurus elephas were determined from 389 individuals (total tagged 5666) tag-recaptured inside a no-take area of the central western Mediterranean and its surrounding zone. High site association and limited movements in tagged lobsters was observed; 60.4% of lobsters moved less than 2 km from the centre of the area (site of release). No clear relationship between lobster movement pattern and sex or size was observed; however, it seemed that the largest males and females tended to be more resident, thus contributing to the rebuilding of the biomass of local lobsters. Most lobsters undertook migrations in the southwest direction. The increased availability of shelters and food towards the southwest could have contributed to the lobsters’ movement. The results of our research indicate that the small size of the protected area and the scale of the movement exhibited by tagged lobsters allows a proportion of the lobster population to move out of the protected area and become susceptible to capture in the adjacent fishery.Se determinaron las pautas de movimiento de 389 individuos de la langosta Palinurus elephas (total marcados 5666) marcados y recapturados en el interior de un área protegida (sin captura) así como de su área circundante. En las langostas marcadas se observó una elevada asociación al lugar de liberación, así como movimientos limitados: el 60.4% de las langostas se movieron menos de 2 km desde el centro del área de liberación. No se observó ninguna clara relación entre las pautas de movimiento de las langostas y su sexo y talla; no obstante, hay una tendencia a que los machos y hembras grandes muestren un comportamiento más residente, contribuyendo así al aumento de la biomasa local. La mayor parte de langostas mostró migraciones en dirección sudoeste. La mayor disponibilidad de refugios y alimento hacia el sudoeste pudo haber contribuído al movimiento de las langostas. Los resultados de este estudio indican que el pequeño tamaño del área protegida y la escala de movimientos mostrados por las langostas marcadas permite a una porción de la población su salida del área protegida y su susceptibilidad a la captura en la pesquería adyacente

Disagreement between parents and health professionals regarding pain intensity in critically ill neonates

OBJECTIVE: To verify whether parents and health professionals homogeneously evaluate presence and intensity of neonatal pain. METHODS: This cross-sectional study enrolled 52 neonates and 154 adults. Inclusion criteria for neonates were admission to neonatal intensive care unit, presence of gastric tube, tracheal tube, and venous lines. Each newborn was observed by a different group of three adults (parent, nurse assistant and pediatrician) for 1 minute at the same time to evaluate presence and intensity of infant's pain. Homogeneity of pain evaluation was analyzed by a modified Bland-Altman plot and by intraclass correlation coefficient (ICC). Multiple linear regression analysis was used to evaluate association of neonatal characteristics and heterogeneity of pain scores for adults. RESULTS: ICC showed disagreement of the pain scores given by the three groups of adults (ICC 0.066, agreement > 0.75). Bland-Altman analysis showed agreement among adults when they thought pain was absent. When they thought pain was present, there was heterogeneity of opinions regarding intensity of neonatal pain. Multiple regression analysis indicated that 10% of this disagreement could be explained by infant's gender and mode of delivery. CONCLUSIONS: Disagreement among adults about intensity of neonatal pain is a marker of the difficulty in deciding the need for analgesia in preverbal patients.OBJETIVO: Verificar se pais e profissionais de saúde que trabalham em unidades de terapia intensiva neonatal avaliam de maneira semelhante a presença e a magnitude da dor no recém-nascido (RN). MÉTODOS: Estudo transversal com 52 RN e 154 adultos. Os critérios de inclusão foram: internação em unidade de terapia intensiva neonatal, presença de sonda gástrica, cânula traqueal e acesso venoso. Cada RN foi observado de modo simultâneo por um trio diferente de adultos (pai/mãe, pediatra e auxiliar de enfermagem) durante 1 minuto para avaliar presença e intensidade da dor do paciente. A análise quanto à homogeneidade da avaliação de dor foi realizada por meio do gráfico de Bland-Altman modificado e do coeficiente de correlação intraclasses (CCI). A associação de fatores próprios do recém-nascido com a heterogeneidade da avaliação da dor do RN pelos adultos foi avaliada por meio de regressão linear múltipla. RESULTADOS: O CCI mostrou discordância entre os três grupos de adultos quanto à avaliação da dor (CCI 0,066, concordância > 0,75). A análise de Bland-Altman mostrou que houve concordância entre os adultos quanto à ausência de dor no RN. Porém, quando os adultos achavam que a dor estava presente, houve heterogeneidade na avaliação da intensidade de dor neonatal. A análise de regressão múltipla indicou que apenas 10% desta heterogeneidade foi explicada pelo sexo e via de parto do RN. CONCLUSÕES: A heterogeneidade na avaliação feita por adultos da intensidade da dor de RN é um marcador da dificuldade de se decidir a respeito da necessidade de analgesia em pacientes pré-verbais.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Divisão de Pediatria NeonatalUNIFESP-EPM Departamento de EpidemiologiaUNIFESP, EPM, Divisão de Pediatria NeonatalUNIFESP, EPM Depto. de EpidemiologiaSciEL

Enhancement of lysosomal glycohydrolase activity in human primary B lymphocytes during spontaneous apoptosis.

It has been shown that lysosomes are involved in B cell apoptosis but lysosomal glycohydrolases have never been investigated during this event. In this study we determined the enzymatic activities of some lysosomal glycohydrolases in human tonsil B lymphocytes (TBL) undergoing in vitro spontaneous apoptosis. Fluorimetric methods were used to evaluate the activities of β-hexosaminidases, α-mannosidase, β-mannosidase, β-galactosidase, β-glucuronidase and α-fucosidase. Results show that in TBL during spontaneous apoptosis, there is a significant increase in the activity of β-hexosaminidases, α-mannosidase, β-mannosidase and β-galactosidase. Also β-glucuronidase and α-fucosidase activities increase but not in a significant manner. Further studies on β-hexosaminidases revealed that also mRNA expression of the α- and β-subunits, which constitute these enzymes, increases during spontaneous TBL apoptosis. When TBL are protected from apoptosis by the thiol molecule N-acetyl-L-cysteine (NAC), there is no longer any increase in glycohydrolase activities and mRNA expression of β-hexosaminidase α- and β-subunits. This study demonstrates for the first time that the activities and expression of some lysosomal glycohydrolases are enhanced in TBL during spontaneous apoptosis and that these increases are prevented when TBL apoptosis is inhibited

Collybistin and gephyrin are novel components of the eukaryotic translation initiation factor 3 complex

<p>Abstract</p> <p>Background</p> <p>Collybistin (CB), a neuron-specific guanine nucleotide exchange factor, has been implicated in targeting gephyrin-GABA_Areceptors clusters to inhibitory postsynaptic sites. However, little is known about additional CB partners and functions.</p> <p>Findings</p> <p>Here, we identified the p40 subunit of the eukaryotic translation initiation factor 3 (eIF3H) as a novel binding partner of CB, documenting the interaction in yeast, non-neuronal cell lines, and the brain. In addition, we demonstrated that gephyrin also interacts with eIF3H in non-neuronal cells and forms a complex with eIF3 in the brain.</p> <p>Conclusions</p> <p>Together, our results suggest, for the first time, that CB and gephyrin associate with the translation initiation machinery, and lend further support to the previous evidence that gephyrin may act as a regulator of synaptic protein synthesis.</p

Conservation and management of Salmo (trutta) macrostigma in Sardinian freshwathers: first results of genetic characterization

Salmonids are present in Sardinia with the autochthonous form Salmo (trutta) macrostigma and the allochthonous ones S. (trutta) trutta and Oncorhynchus mykiss. For many years repeated and uncontrolled introduction of S. (trutta) trutta of doubtful origin, have been carried out; this has enhanced hybridization and genetic pollution phenomena, progressively determining the contraction, or even the disappearance of the original S. (trutta) macrostigma populations. Currently, the majority of autochthonous fish populations, depending exclusively on natural reproduction, have disappeared from some water courses, or else have become very rare. In an attempt to satisfy the requirements of safeguarding biodiversity, the Department of Animal Biology and Ecology, University of Cagliari, together with the “SarcidanoBarbagia di Seulo” mountain district authorities, has recently set up a research program aiming at restocking the Sardinian trout populations. Sampling was carried out at four sites located in two different areas of the Flumendosa hydrographic basin in central-western Sardinia. A total of 30 trout, captured between June and November 2005, was measured, weighed, photographed and submitted to DNA mitochondrial analysis (amplifying and sequencing a portion of the D-loop control region) and of the LDH-C1* nuclear gene (amplifying and digesting the amplified part with the Bsl1 enzyme). Valid candidates for use as broodstock were considered to be those individuals found to possess an autochthonous mitochondrial haplotype (haplotype Ad, according to Bernatchez) and to be homozygous for the 100 allele for LDH-C1*, that enables the contribution of genes of Atlantic origin to be excluded. In the remaining individuals, the presence of allele 90 of northern European origin was found in homozygosity or heterozygosity, and also of the Atlantic mitochondrial haplotype (haplotype AT, according to Bernatchez). The present paper reports the first findings to have emerged from the genetic investigations, and confirms the validity of the method for distinguishing the salmonid populations