Comparative analysis of physical development and functional capacity of different sports athletes during competition period

The aim of this study was to compare physical fitness of high performance canoeists, rowers, Greek-Roman style wrestlers, basketball players and skiers during their competition period. Indices of physical development, muscle and fat mass and their ratios were obtained. Single muscular contraction power (SMCP) and anaerobic alactic muscular power (AAMP) were also measured. The anaerobic glycolytic power (AGP) was estimated by ergometer. The Bosco methodology was used to estimate the activity of fast twitch fibres (FTF). The psychomotor response time (PRT) and movement frequency (MF) per 10 s were estimated and Roufier index (RI) was applied to measure functional capacity of circulatory and respiratory systems. The examination of athletes specialising in five different sports allowed for identification of the peculiarities of sports specialisation. The distinctive height, highest body weight and static hand power values characterised rowers and basketball players; while canoeists had the highest muscle mass. Only canoeists achieved high SMCP during the competition period. The SMCP of rowers and skiers was optimal, whereas the basketball players and wrestlers demonstrated an insufficient single muscular contraction power. The highest anaerobic alactic muscle power was observed in basketball players and canoeists, whereas in the muscles the basketball players and wrestlers the activity of FTF was insufficient. Though its parameters were approximate to endurance-trained rowers, they considerably fell behind those of canoeists. The functional capacity of circulatory and respiratory system of skiers was highest. The research revealed that the majority of indices of skiers and wrestlers’ physical fitness were lowest among the other studied athletes. Such results reflect their limited potential to achieve high results in international competitions

SHIRAZ: an automated histology image annotation system for zebrafish phenomics

Histological characterization is used in clinical and research contexts as a highly sensitive method for detecting the morphological features of disease and abnormal gene function. Histology has recently been accepted as a phenotyping method for the forthcoming Zebrafish Phenome Project, a large-scale community effort to characterize the morphological, physiological, and behavioral phenotypes resulting from the mutations in all known genes in the zebrafish genome. In support of this project, we present a novel content-based image retrieval system for the automated annotation of images containing histological abnormalities in the developing eye of the larval zebrafish

Knock-Down of Cathepsin D Affects the Retinal Pigment Epithelium, Impairs Swim-Bladder Ontogenesis and Causes Premature Death in Zebrafish

The lysosomal aspartic protease Cathepsin D (CD) is ubiquitously expressed in eukaryotic organisms. CD activity is essential to accomplish the acid-dependent extensive or partial proteolysis of protein substrates within endosomal and lysosomal compartments therein delivered via endocytosis, phagocytosis or autophagocytosis. CD may also act at physiological pH on small-size substrates in the cytosol and in the extracellular milieu. Mouse and fruit fly CD knock-out models have highlighted the multi-pathophysiological roles of CD in tissue homeostasis and organ development. Here we report the first phenotypic description of the lack of CD expression during zebrafish (Danio rerio) development obtained by morpholino-mediated knock-down of CD mRNA. Since the un-fertilized eggs were shown to be supplied with maternal CD mRNA, only a morpholino targeting a sequence containing the starting ATG codon was effective. The main phenotypic alterations produced by CD knock-down in zebrafish were: 1. abnormal development of the eye and of retinal pigment epithelium; 2. absence of the swim-bladder; 3. skin hyper-pigmentation; 4. reduced growth and premature death. Rescue experiments confirmed the involvement of CD in the developmental processes leading to these phenotypic alterations. Our findings add to the list of CD functions in organ development and patho-physiology in vertebrates

Field Measurements of Gasoline Direct Injection Emission Factors: Spatial and Seasonal Variability

Four field campaigns were conducted between February 2014 and January 2015 to measure emissions from light-duty gasoline direct injection (GDI) vehicles (2013 Ford Focus) in an urban near-road environment in Toronto, Canada. Measurements of CO₂, CO, NO_<i>x</i>, black carbon (BC), benzene, toluene, ethylbenzene-xylenes (BTEX), and size-resolved particle number (PN) were recorded 15 m from the roadway and converted to fuel-based emission factors (EFs). Other than for NO_<i>x</i> and CO, the GDI engine had elevated emissions compared to the Toronto fleet, with BC EFs in the 73rd percentile, BTEX EFs in the 80–90th percentile, and PN EFs in the 75th percentile during wintertime measurements. Additionally, for three campaigns, a second platform for measuring PN and CO₂ was placed 1.5–3 m from the roadway to quantify changes in PN with distance from point of emission. GDI vehicle PN EFs were found to increase by up to 240% with increasing distance from the roadway, predominantly due to an increasing fraction of sub-40 nm particles. PN and BC EFs from the same engine technology were also measured in the laboratory. BC EFs agreed within 20% between the laboratory and real-world measurements; however, laboratory PN EFs were an order of magnitude lower due to exhaust conditioning

Embedding, serial sectioning and staining of zebrafish embryos using JB-4 resin

Histological techniques are critical for observing tissue and cellular morphology. Here, we outline our protocol for embedding, serial sectioning, staining, and visualizing zebrafish embryos embedded in JB-4™ plastic resin – a glycol methacrylate-based medium that results in excellent preservation of tissue morphology. In addition we describe our procedures for staining plastic sections with Toluidine Blue or Hematoxylin and Eosin (H&E), and show how to couple these stains with whole-mount RNA in situ hybridization. We also describe how to maintain and visualize immunofluorescence and GFP signals in JB-4™ resin. The protocol we outline – from embryo preparation, embedding, sectioning and staining to visualization - can be accomplished in three days. Overall, we reinforce that plastic embedding can provide higher resolution of cellular details and is a valuable tool for cellular and morphological studies in zebrafish