Alleviative effects of pinostrobin against cadmium-induced renal toxicity in rats by reducing oxidative stress, apoptosis, inflammation, and mitochondrial dysfunction

IntroductionCadmium (Cd) is a highly toxic heavy metal that can be found everywhere in the environment and can have harmful effects on both human and animal health. Pinostrobin (PSB) is a bioactive natural flavonoid isolated from Boesenbergia rotunda with several pharmacological properties, such as antiinflammatory, anticancer, antioxidant, and antiviral. This investigation was intended to assess the therapeutic potential of PSB against Cd-induced kidney damage in rats.MethodsIn total, 48 Sprague Dawley rats were divided into four groups: a control, a Cd (5 mg/kg), a Cd + PSB group (5 mg/kg Cd and 10 mg/kg PSB), and a PSB group (10 mg/kg) that received supplementation for 30 days.ResultsExposure to Cd led to a decrease in the activities of catalase (CAT), glutathione reductase (GSR), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX), whereas levels of reactive oxygen species (ROS) and malondialdehyde (MDA) increased. Cd exposure also caused a substantial increase in urea, kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL), and creatinine levels. Moreover, a noticeable decline was noticed in creatinine clearance. Moreover, Cd exposure considerably increased the levels of inflammatory indices, including interleukin-1b (IL-1b), tumor necrosis factor-a (TNF-a), interleukin-6 (IL-6), nuclear factor kappa-B (NF-kB), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) activity. Cd treatment decreased the expression of the antiapoptotic markers (Bcl-2) while increasing the expression of apoptotic markers (Bax and Caspase-3). Furthermore, Cd treatment substantially reduced the TCA cycle enzyme activity, such as alpha-ketoglutarate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, and isocitrate dehydrogenase. Moreover, mitochondrial electron transport chain enzymes, succinatedehydrogenase, NADH dehydrogenase, cytochrome c-oxidase, and coenzyme Q-cytochrome reductase activities were also decreased following Cd exposure. PSB administration substantially reduced the mitochondrial membrane potential while inducing significant histological damage. However, PSB treatment significantly reduced Cd-mediated renal damage in rats.ConclusionThus, the present investigation discovered that PSB has ameliorative potential against Cd-induced renal dysfunction in rats

In vitro assessment of the protection from oxidative stress by various fractions of Artemisia incisa Pamp.

The methanolic extract of Artemisia incisa Pamp. was dissolved in distilled water and successively partitioned with n-hexane, chloroform, ethyl acetate and n-butanol. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods, i.e., the scavenging activity of the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), the total antioxidant activity, the ferric reducing antioxidant power (FRAP) assay and the ferric thiocyanate assay. In addition, the total phenolics was determined. The obtained results revealed that among the studied fractions the ethyl acetate soluble fraction showed the most potent DPPH-radical scavenging activity with an IC50 value of 5.3 ± 0.71 μg mL-1, which is even more effective than the standard antioxidant butylated hydroxytoluene (BHT) (IC50 value 0f 12.1± 0.92 μg mL-1). The ethyl acetate fraction also showed the highest FRAP value (3677.13 ± 27.1 μg TE mL-1), inhibition of lipid peroxidation (60.93 ± 0.84 % at 500 μg mL-1) and total phenolic content (95.5 ± 0.05 μg GAE g-1) as compared to other fractions. However, the remaining aqueous fraction was found to posses the highest antioxidant activity of all the fractions

Performance Evaluation of Bus Rapid Transit System: A Comparative Analysis of Alternative Approaches for Energy Efficient Eco-Friendly Public Transport System

The development of the bus rapid transit system (BRTS) is tremendously growing in developing countries of the world. In large cities, the projection of transportation intends to enhance economic growth and changes the image of the city for both residents and outsiders. The purpose of this research was to study the application of alternative options for energy efficient BRTS in developing countries. The BRTS has some of its accessibility patterns that relate to the socio-economic strata. A decision-making efficiency analysis methodology has been applied to analyze the comparative analysis of both conventional fuel and hybrid bus systems for the Multan city of Pakistan. The section-wise application of a hybrid energy-based bus system has been analyzed in comparison to the conventional bus system. Out of 21 stations, the efficiency-wise hybrid bus system remained superior or equivalent to the standard value of 1 except one midpoint section. The finding of the analysis indicates that the hybrid mechanism of buses can not only replace a conventional fuel-based system, but will also help as an energy-efficient and eco-friendly economical solution. This study will help to revolutionize the bus rapid transit system in developing countries

Sustainable FRP-Confined Symmetric Concrete Structures: An Application Experimental and Numerical Validation Process for Reference Data

The use of fiber-reinforced polymer (FRP) jackets as external confinement is becoming popular, especially in seismic areas, because of its ability to enhance the strength and ductility of reinforced concrete to perform as a sustainable symmetric structural member. Therefore, various researchers have worked out for the prediction of strength and strain models of FRP-confined concrete. This study presents the improved strain models for the FRP confined cylindrical concrete members. Different previously proposed models of axial strain of FRP-confined concrete were evaluated based on a large database of 678 specimens from previous experiments and an improved model was proposed using the general regression analysis technique. Furthermore, the proposed model was validated using the previous experimental work of FRP-wrapped concrete cylinders and their finite elements analysis (FEA) using the ABAQUS software. The accuracy of the proposed strain model was quite satisfactory in comparison with the previous experimental and FEA results of the present study. Moreover, the proposed empirical strain model was used for the parametric study to investigate the effect of different geometric and material parameters such as the compressive strength of unconfined concrete, diameter of the cylinder, elastic modulus and thickness of the FRP layers, on the axial strain of FRP-wrapped cylinders. A close agreement among the proposed strain models and experimental outputs was observed. This study will help in understanding the behavior of sustainable FRP-confined symmetric concrete members