Joint VNF Placement and CPU Allocation in 5G

Thanks to network slicing, 5G networks will support a variety of services in a flexible and swift manner. In this context, we seek to make high-quality, joint optimal decisions concerning the placement of VNFs across the physical hosts for realizing the services, and the allocation of CPU resources in VNFs sharing a host. To this end, we present a queuing-based system model, accounting for all the entities involved in 5G networks. Then, we propose a fast and efficient solution strategy yielding nearoptimal decisions.We evaluate our approach in multiple scenarios that well represent real-world services, and find it to consistently outperform state-of-the-art alternatives and closely match the optimum.This work is partially supported by the European Commission through the H2020 5G-TRANSFORMER project (Project ID 761536)En prens

On the dynamics of the adenylate energy system: homeorhesis vs homeostasis.

Biochemical energy is the fundamental element that maintains both the adequate turnover of the biomolecular structures and the functional metabolic viability of unicellular organisms. The levels of ATP, ADP and AMP reflect roughly the energetic status of the cell, and a precise ratio relating them was proposed by Atkinson as the adenylate energy charge (AEC). Under growth-phase conditions, cells maintain the AEC within narrow physiological values, despite extremely large fluctuations in the adenine nucleotides concentration. Intensive experimental studies have shown that these AEC values are preserved in a wide variety of organisms, both eukaryotes and prokaryotes. Here, to understand some of the functional elements involved in the cellular energy status, we present a computational model conformed by some key essential parts of the adenylate energy system. Specifically, we have considered (I) the main synthesis process of ATP from ADP, (II) the main catalyzed phosphotransfer reaction for interconversion of ATP, ADP and AMP, (III) the enzymatic hydrolysis of ATP yielding ADP, and (IV) the enzymatic hydrolysis of ATP providing AMP. This leads to a dynamic metabolic model (with the form of a delayed differential system) in which the enzymatic rate equations and all the physiological kinetic parameters have been explicitly considered and experimentally tested in vitro. Our central hypothesis is that cells are characterized by changing energy dynamics (homeorhesis). The results show that the AEC presents stable transitions between steady states and periodic oscillations and, in agreement with experimental data these oscillations range within the narrow AEC window. Furthermore, the model shows sustained oscillations in the Gibbs free energy and in the total nucleotide pool. The present study provides a step forward towards the understanding of the fundamental principles and quantitative laws governing the adenylate energy system, which is a fundamental element for unveiling the dynamics of cellular life

Electrification of Diesel-Based Powertrains for Heavy Vehicles

In recent decades as environmental concerns and the cost and availability of fossil fuels have become more pressing issues, the need to extract more work from each drop of fuel has increased accordingly. Electrification has been identified as a way to address these issues in vehicles powered by internal combustion engines, as it allows existing engines to be operated more efficiently, reducing overall fuel consumption. Two applications of electrification are discussed in the work presented: a series-electric hybrid powertrain from an on-road class 8 truck, and an electrically supercharged diesel engine for use in the series hybrid power system of a wheel loader. The first application is an experimental powertrain developed by a small start-up company for use in highway trucks. The work presented in this thesis shows test results from routes along (1) Interstate 75 between Florence, KY, and Lexington, KY, and (2) Interstates 74 and 70 east of Indianapolis, during which tests the startup collected power flow data from the vehicle’s motor, generator, and battery, and three-dimensional position data from a GPS system. Based on these data, it was determined that the engine-driven generator provided an average of 15% more propulsive energy than required due to electrical losses in the drivetrain. Some of these losses occured in the power electronics, which are shown to be 82% - 92% efficient depending on power flow direction, but the battery showed significant signs of wear, accounting for the remainder of these electrical losses. Overall, most of the system’s fuel savings came from its regenerative braking capability, which recaptured between 3% and 12% of the total drive energy output. Routes with significant grade changes maximize this energy recapture percentage, but it is shown minimizing drag and rolling resistance with a more modern truck and trailer could further increase this energy capture to between 8% and 18%. In the second application, an electrified air handling system is added to a 4.5L engine, allowing it to replace the 6.8L engine in John Deere’s 644K hybrid wheel loader. Most of the fuel savings arise from downsizing the engine, so in this case an electrically driven supercharger (eBooster) allows the engine to meet the peak torque requirements of the larger, original engine. In this thesis, a control-oriented nonlinear state space model of the modified 4.5L engine is presented and linearized for use in designing a robust, multi-input multi-output (MIMO) controller which commands the engine’s fueling rate, eBooster, eBooster bypass valve, exhaust gas recirculation (EGR) valve, and exhaust throttle. This integrated control strategy will ultimately allow superior tracking of engine speed, EGR fraction, and airfuel ratio (AFR) targets, but these performance gains over independent single-input singleoutput control loops for each component demand linear models that accurately represent the engine’s gas exchange dynamics. To address this, a physics-based model is presented and linearized to simulate pressures, temperatures, and shaft speeds based on sub-models for exhaust temperature, cylinder charge flow, valve flow, compressor flow, turbine flow, compressor power, and turbine power. The nonlinear model matches the truth reference engine model over the 1200 rpm - 2000 rpm and 100 Nm - 500 Nm speed and torque envelope of interest within 10% in steady state and 20% in transient conditions. Two linear models represent the full engine’s dynamics over this speed and torque range, and these models match the truth reference model within 20% in the middle of the operating envelope. However, specifically at (1) low load for any speed and (2) high load at high speed, the linear models diverge from the nonlinear and truth reference models due to nonlinear engine dynamics lost in linearization. Nevertheless, these discrepancies at the edges of the engine’s operating envelope are acceptable for control design, and if greater accuracy is needed, additional linear models can be generated to capture the engine’s dynamics in this region

Regulation of protein synthesis during early limitation of Saccharomyces cerevisiae

Arsenate, a competitive inhibitor with phosphate in phosphorylation reactions, has been used to lower adenine and guanine nucleotide levels in Saccharomyces cerevisiae to study nucleotide effects on protein synthesis. By measuring polysome levels, we have shown that initiation of protein synthesis is much more sensitive than elongation or termination to inhibition when the ATP/ADP, GTP/GDP ratios are low. When the arsenate-phosphate molar ratio was 0.27, protein synthesis was inhibited by about 85% and the kinetics of polysome decay was similar to that observed with the initiation inhibitor, verrucarin-76, or with the protein synthesis initiation mutant, ts187, at the restrictive temperature. With this level of arsenate, the adenylate energy charge dropped from 0.9 to 0.7 and the ATP/ADP and GTP/GDP ratios dropped from 6 to 2. The observed correlations between nucleotide ratio changes and inhibition of protein synthesis suggest that the former may be a control signal for the latter. The significance of these in vivo correlations will have to be tested with an in vitro protein synthesizing system. Higher arsenate levels resulted in even lower ATP/ADP, GTP/GDP ratios and in a slower decay of polysomes, implying that, eventually, elongation (in addition to initiation) was being inhibited.</jats:p