Susceptibilidade antimicrobiana de cepas de estafilococcos coagulase-negativa isolados de ovinos de corte com mastite

Avaliou-se a sensibilidade antimicrobiana in vitro de 121 cepas de estafilococos coagulase-negativa isolada de leite de ovelhas Santa Inês, aos fármacos: penicilina, amoxicilina, ampicilina, estreptomicina, oxaciclina, neomicina, cefalotina, gentamicina e sulfonamida. A resistência à sulfonamida foi a mais frequente (27,3%), seguida pela estreptomicina (14,0%) e pela oxaciclina (14,0%), enquanto da gentamicina (1,6%) foi a menos frequente. Todas as cepas foram sensíveis a pelo menos um antimicrobiano, e 20,3% das cepas apresentaram resistência múltipla. Os resultados mostram a importância de Staphylococci coagulase-negativas como agentes causadores de mastite em ovinos, e o perfil de resistência múltipla indica a importância da determinação da resistência à oxaciclina como indicador da presença de ilhas de patogenicidade que contêm fatores de virulência e resistência a outros antimicrobianos que contribuem para a sobrevivência da bactéria ao tratamento.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Measurement of the ttbar Production Cross Section in ppbar Collisions at sqrt(s)=1.96 TeV using Lepton + Jets Events with Lifetime b-tagging

We present a measurement of the top quark pair ($t\bar{t}$) production cross section ($\sigma_{t\bar{t}}$) in $p\bar{p}$ collisions at $\sqrt{s}=1.96$ TeV using 230 pb$^{-1}$ of data collected by the D0 experiment at the Fermilab Tevatron Collider. We select events with one charged lepton (electron or muon), missing transverse energy, and jets in the final state. We employ lifetime-based b-jet identification techniques to further enhance the $t\bar{t}$ purity of the selected sample. For a top quark mass of 175 GeV, we measure $\sigma_{t\bar{t}}=8.6^{+1.6}_{-1.5}(stat.+syst.)\pm 0.6(lumi.)$ pb, in agreement with the standard model expectation.Comment: 7 pages, 2 figures, 3 tables Submitted to Phys.Rev.Let

Pre-M Phase-promoting Factor Associates with Annulate Lamellae in Xenopus Oocytes and Egg Extracts

We have used complementary biochemical and in vivo approaches to study the compartmentalization of M phase-promoting factor (MPF) in prophase Xenopus eggs and oocytes. We first examined the distribution of MPF (Cdc2/CyclinB2) and membranous organelles in high-speed extracts of Xenopus eggs made during mitotic prophase. These extracts were found to lack mitochondria, Golgi membranes, and most endoplasmic reticulum (ER) but to contain the bulk of the pre-MPF pool. This pre-MPF could be pelleted by further centrifugation along with components necessary to activate it. On activation, Cdc2/CyclinB2 moved into the soluble fraction. Electron microscopy and Western blot analysis showed that the pre-MPF pellet contained a specific ER subdomain comprising "annulate lamellae" (AL): stacked ER membranes highly enriched in nuclear pores. Colocalization of pre-MPF with AL was demonstrated by anti-CyclinB2 immunofluorescence in prophase oocytes, in which AL are positioned close to the vegetal surface. Green fluorescent protein-CyclinB2 expressed in oocytes also localized at AL. These data suggest that inactive MPF associates with nuclear envelope components just before activation. This association may explain why nuclei and centrosomes stimulate MPF activation and provide a mechanism for targeting of MPF to some of its key substrates

Genetic Divergence Among Accessions Of Cassava (manihot Esculenta Crantz) Sampled In The Tapajós Region, State Of Pará, Using Agronomic Characters And Microsatellite Markers

The aim of this work was to estimate the genetic divergence among accessions of cassava sampled in the Tapajós region in the State of Pará, Brazil, and conserved at the Regional Germplasm Bank of Eastern Amazon, using agronomic descriptors and molecular markers. Twenty-two accessions of cassava were evaluated in the field for two successive years, based on six agronomic descriptors in twelve-months-old plants without a specific experimental design. Accessions were also evaluated with eleven microsatellite loci in an automatic DNA analyser. Descriptive and multivariate statistical analyses were applied. Based on principal components analysis, the character weight of the aerial portion of the plant contributed most to the phenotypical variation. The six traits were used in the analysis of genetic dissimilarity between accessions, and the correlation between matrices generated by morphological and molecular data was estimated. The matrices of genetic dissimilarity were used in the construction of dendrograms using the UPGMA method. We observed a high variation of agronomical descriptors and molecular markers evaluated, which were capable to separate the accessions into distinct groups. A weak positive correlation was detected among the two matrices of genetic distances, which indicates the possibility to explore the genetic diversity using crossings and accessions Amarelinha 36 and Olho roxo 13 are divergent and potentially promising for the generation of heterotic hybrids.3752989300

Yield And Nutritive Value Of The Silage Of Corn Intercropped With Tropical Perennial Grasses

The objective of this work was to compare the yield and nutritive value of the silage of corn intercropped with palisade grass (Urochloa brizantha 'Marandu') or guinea grass (Megathyrsus maximus 'Tanzânia') with those of the silage of monocropped corn, as well as to evaluate the pasture established in the intercropping systems during two growing seasons (2010-2011 and 2011-2012), in low-altitude Brazilian Cerrado. The treatments consisted of three cropping systems: monocropped corn, corn intercropped with palisade grass, and corn intercropped with guinea grass, with four replicates. Intercropping decreased corn forage dry matter yield for silage; however, due to the addition of grass, total dry matter yield (corn + grass) was similar between treatments. Intercropping also did not negatively affect corn production components and morphological characteristics. The cropping systems provided silages with good nutritive values, and the inclusion of tropical forages increased the silage fiber contents. Intercropping corn with tropical perennial grasses is a viable option for producing large quantities of silage with good nutritive value. The forage yield (silage + pasture) of these intercropping systems is similar to that of monocropped corn in tropical regions and has the advantage of providing a pasture in the off-season.521637

Exclusive Studies of Charged-Particle Emission in 1-H and 3-He-Induced Reactions on Heavy Nuclei

This research was sponsored by the National Science Foundation Grant NSF PHY-931478

Search for electroweak production of single top quarks in ppˉp\bar{p} collisions.

We present a search for electroweak production of single top quarks in the electron+jets and muon+jets decay channels. The measurements use ~90 pb^-1 of data from Run 1 of the Fermilab Tevatron collider, collected at 1.8 TeV with the DZero detector between 1992 and 1995. We use events that include a tagging muon, implying the presence of a b jet, to set an upper limit at the 95% confidence level on the cross section for the s-channel process ppbar->tb+X of 39 pb. The upper limit for the t-channel process ppbar->tqb+X is 58 pb. (arXiv

Search for new particles in the two-jet decay channel with the D0 detector

We present the results of a search for the production of new particles decaying into two jets in pbarp collisions at sqrt{s} = 1.8 TeV, using the DZero 1992-1995 data set corresponding to 109 pb^-1. We exclude at the 95% confidence level the production of excited quarks (q*) with masses below 775 GeV/c^2, the most restictive limit to date. We also exclude standard-model-like W' (Z') bosons with masses between 300 and 800 GeV/c^2 (400 and 640 GeV/c^2). A W' boson with mass $<$ 300 GeV/c^2 has been excluded by previous measurements, and our lower limit is therefore the most stringent to date

Perfil dos Usuários de Medicamentos das Unidades Básicas de Saúde de Ceres-GO.

Foram aplicados 321 questionários aos usuários de medicamentos nas seis unidades básicas de saúde da cidade de Ceres – GO.  Os dados foram analisados estatisticamente pelo software EPI INFO 3.5.2 e Microsoft Office Excel® 2007. O perfil dos entrevistados demonstra que a população estudada é carente de informações quanto ao uso correto de medicamentos, sendo a ausência do farmacêutico uma das principais causas deste problema. Portanto esta pesquisa fornece subsídios importantes para o estabelecimento de estratégias específicas e políticas públicas como a Política Nacional de Medicamentos e a Política Nacional de Assistência Farmacêutica, visando à melhoria da saúde da população. Abstract 321 questionnaires were applied to drug users in the six basic health units in the city of Ceres - GO. Data were statistically analyzed by using EPI INFO 3.5.2 and Microsoft Office Excel ® 2007. The profile of respondents shows that the population is deprived of information concerning the correct use of medicines, and the absence of the pharmacist a major cause of this problem. Therefore this research provides important support for the establishment of specific strategies and policies such as the National Drug Policy and the National Policy for Pharmaceutical Care, aiming to improve the population's health