A Census of the Carina Nebula -- II. Energy Budget and Global Properties of the Nebulosity

The first paper in this series took a direct census of energy input from the known OB stars in the Carina Nebula, and in this paper we study the global properties of the surrounding nebulosity. We find that the total IR luminosity of Carina is about 1.2E7 Lsun, accounting for only about 50-60% of the known stellar luminosity from Paper I. Similarly, the ionizing photon luminosity -- (abridged; many important details omitted). Synchronized star formation around the periphery of Carina provides a strong case that star formation here was indeed triggered by stellar winds and UV radiation. This second generation appears to involve a cascade toward preferentially intermediate- and low-mass stars, but this may soon change when eta Car and its siblings explode. If the current reservoir of atomic and molecular gas can be tapped at that time, massive star formation may be rejuvinated around the periphery of Carina much as if it were a young version of Gould's Belt. Also, when these multiple SNe occur, the triggered second generation will be pelted repeatedly with SN ejecta bearing short-lived radioactive nuclides. Carina may therefore represent the most observable analog to the cradle of our own Solar System.Comment: MNRAS accepted, 14 pages, fig 1 in colo

The ethics of sociocultural risk research

In socio-cultural risk research, an epistemological tension often follows if real hazards in the world are juxtaposed against the essentially socially constructed nature of all risk. In this editorial, we consider how this paradox is manifest at a practical level in a number of ethical dilemmas for the risk researcher. (1) In terms of strategies for seeking informed consent, and for addressing the power inequalities involved in interpretative and analytical work, researchers can find themselves pushing at the boundaries of standard understandings of ethical practices and ways of engaging informants in their studies. (2) Impact on participants is another key area of concern, since the subject matter on which data are collected in risk research may be a source of uncertainty, anxiety or unwanted self knowledge. (3) Risk researchers also face the possibility of institutional repercussions of raising risk issues with people who usually normalize the risks, thereby stimulating distrust in the institutions or organizations with formal responsibilities for risk management. There are no simple formulae to guide the researcher in dealing with such ethical issues and paradoxes. It is important, though, to recognize their specificity in risk studies, including the ambiguous status of questions about vulnerability since judgements about 'who is vulnerable' and 'in what ways' are themselves influenced by the situational framings and understandings of participants and researchers

Structure, substrate recognition and reactivity of Leishmania major mevalonate kinase

This research was supported by the German Academic Exchange Service (DAAD), the Wellcome Trust (TKS and WNH as Trust Senior Research fellows), the Biotechnology and Biological Science Research Council (Structural Proteomics of Rational Targets) and the European Synchrotron Radiation Facility.Background: Isoprenoid precursor synthesis via the mevalonate route in humans and pathogenic trypanosomatids is an important metabolic pathway. There is however, only limited information available on the structure and reactivity of the component enzymes in trypanosomatids. Since isoprenoid biosynthesis is essential for trypanosomatid viability and may provide new targets for therapeutic intervention it is important to characterize the pathway components. Results: Putative mevalonate kinase encoding genes from Leishmania major (LmMK) and Trypanosoma brucei (TbMK) have been cloned, over-expressed in and proteins isolated from procyclic-form T. brucei. A highly sensitive radioactive assay was developed and shows ATP-dependent phosphorylation of mevalonate. Apo and (R)-mevalonate bound crystal structures of LmMK, from a bacterial expression system, have been determined to high resolution providing, for the first time, information concerning binding of mevalonate to an MK. The mevalonate binds in a deep cavity lined by highly conserved residues. His25 is key for binding and for discrimination of (R)-over (S)-mevalonate, with the main chain amide interacting with the C3 hydroxyl group of ( R)mevalonate, and the side chain contributing, together with Val202 and Thr283, to the construction of a hydrophobic binding site for the C3 methyl substituent. The C5 hydroxyl, where phosphorylation occurs, points towards catalytic residues, Lys18 and Asp155. The activity of LmMK was significantly reduced compared to MK from other species and we were unable to obtain ATP-binding data. Comparisons with the rat MK:ATP complex were used to investigate how this substrate might bind. In LmMK, helix alpha 2 and the preceding polypeptide adopt a conformation, not seen in related kinase structures, impeding access to the nucleotide triphosphate binding site suggesting that a conformational rearrangement is required to allow ATP binding. Conclusion: Our new structural information, consistent with data on homologous enzymes allows a detailed description of how mevalonate is recognized and positioned for catalysis in MK. The mevalonate-binding site is highly conserved yet the ATP-binding site is structurally distinct in LmMK. We are unable to provide a definitive explanation for the low activity of recombinant protein isolated from a bacterial expression system compared to material isolated from procyclic-form Trypanosoma brucei.Publisher PDFPeer reviewe

Uncertainty in Soft Temporal Constraint Problems:A General Framework and Controllability Algorithms forThe Fuzzy Case

In real-life temporal scenarios, uncertainty and preferences are often essential and coexisting aspects. We present a formalism where quantitative temporal constraints with both preferences and uncertainty can be defined. We show how three classical notions of controllability (that is, strong, weak, and dynamic), which have been developed for uncertain temporal problems, can be generalized to handle preferences as well. After defining this general framework, we focus on problems where preferences follow the fuzzy approach, and with properties that assure tractability. For such problems, we propose algorithms to check the presence of the controllability properties. In particular, we show that in such a setting dealing simultaneously with preferences and uncertainty does not increase the complexity of controllability testing. We also develop a dynamic execution algorithm, of polynomial complexity, that produces temporal plans under uncertainty that are optimal with respect to fuzzy preferences

Charge exchange lifetimes for ions in the magnetosphere

Latest and best measurements of physical quantities involved in complete calculation of the charge exchange lifetime of mirroring magnetospheric ions are coalesced and summarized. It is critical that the charge exchange lifetimes for ions be known as accurately as possible in order to apply the charge exchange mechanism to ion phenomena within the earth's magnetosphere

Approaches to Three-Dimensional Transformation Optical Media Using Quasi-Conformal Coordinate Transformations

We introduce an approach to the design of three-dimensional transformation optical (TO) media based on a generalized quasi-conformal mapping approach. The generalized quasi-conformal TO (QCTO) approach enables the design of media that can, in principle, be broadband and low-loss, while controlling the propagation of waves with arbitrary angles of incidence and polarization. We illustrate the method in the design of a three-dimensional "carpet" ground plane cloak and of a flattened Luneburg lens. Ray-trace studies provide a confirmation of the performance of the QCTO media, while also revealing the limited performance of index-only versions of these devices

Role of the community matron in advance care planning and ‘do not attempt CPR’ decision-making: a qualitative study

The community matron (CM) is often the key worker caring for patients with chronic, life-limiting, long-term conditions, but these patients are not always recognised as palliative cases. This study explored the experiences of CMs with regard to advance care planning (ACP) and ‘do not attempt cardiopulmonary resuscitation’ (DNACPR) decision-making to understand whether or not they felt adequately prepared for this aspect of their role, and why. Qualitative data were generated from six CMs using a broad interpretive phenomenological approach. Face-to-face recorded interviews were analysed using template analysis. The study found that although participants faced complex ethical situations around ACP and DNACPR almost on a daily basis, none had received any formal training despite the emphasis on training in national and local guidelines. Participants often struggled to get their patients accepted on to the Gold Standards Framework. The research found variability and complexity of cases to be the main barriers to clear identification of the palliative phase

Dependence of the charge exchange lifetimes on mirror latitude

The dependence of the charge exchange lifetimes on the mirror latitude for ions mirroring off the geomagnetic equator was re-computed using the improved hydrogen distribution models. The Chamberlain model was used to define the spatial distribution of the neutral hydrogen environment through which the ring current ions traverse. The resultant dependence of the charge exchange lifetime on mirror latitude is best fitted by the approximation that contains the charge exchange lifetime for equatorial particles