FREQUÊNCIAS DOS TIPOS SANGUÍNEOS E TÍTULOS DOS ALOANTICORPOS EM FELINOS DOMÉSTICOS

A área da hemoterapia necessita de testes de compatibilidade sanguínea confiáveis, como a tipagem sanguínea, para se evitarem possíveis reações transfusionais, mas em felinos é também importante para se evitar a isoeritrólise neonatal. Transfusões sanguíneas realizadas entre felinos doadores e receptores que não possuem compatibilidade sanguínea podem refletir em reação transfusional aguda, particularmente severa quando o sangue tipo A é transfundido em um gato tipo B, pois geralmente este último possui altos níveis de aloanticorpos de ocorrência natural. Portanto, o conhecimento da frequência dos tipos sanguíneos da população de gatos de uma região pode auxiliar na determinação dos riscos de reações transfusionais e de ocorrência de isoeritrólise neonatal. Tais riscos podem ser prevenidos com a tipagem sanguínea em casos de transfusão. Foram coletadas 100 amostras sanguíneas de felinos para a realização da tipagem sanguínea com plasmas reagentes anti-A e anti-B conhecidas e titulações de aloanticorpos anti-A e anti-B dos plasmas armazenados. A distribuição das frequências dos grupos sanguíneos foi 96% de felinos com tipo sanguíneo A e 4% de felinos com tipo sanguíneo B, não sendo encontrado na amostra populacional de felino tipo AB. Há um grande risco de reação adversa através da transfusão sanguínea randomizada entre felinos não tipados previamente. Palavras-chave: gatos; imunohematologia; medicina transfusional; tipagem sanguínea

Synthesis of Selenium-Quinone Hybrid Compounds with Potential Antitumor Activity via Rh-Catalyzed C-H Bond Activation and Click Reactions

In continuation of our quest for new redox-modulating catalytic antitumor molecules, selenium-containing quinone-based 1,2,3-triazoles were synthesized using rhodium-catalyzed C-H bond activation and click reactions. All compounds were evaluated against five types of cancer cell lines: HL-60 (human promyelocytic leukemia cells), HCT-116 (human colon carcinoma cells), SF295 (human glioblastoma cells), NCIH-460 (human lung cells) and PC3 (human prostate cancer cells). Some compounds showed good activity with IC50 values below 1 µM. The cytotoxic potential of the naphthoquinoidal derivatives was also evaluated in non-tumor cells, exemplified by L929 cells. Overall, these compounds represent promising new lead derivatives and stand for a new class of chalcogenium-containing derivatives with potential antitumor activity

Potencialization of mesotrione efficiency in tank mixture with inhibiting of Photosystem II by distinct absorption pathways of crabgrass

Com objetivo de avaliar a potencialização da eficiência do mesotrione em mistura em tanque com herbicidas inibidores do Fotossistema II no controle de capim-colchão (Digitaria horizontalis), quatro experimentos foram conduzidos em estufas plásticas pertencentes à Escola Superior de Agronomia de Paraguaçu Paulista, município de Paraguaçu Paulista, Estado de São Paulo, utilizando-se um Latossolo de textura arenosa. Os delineamentos experimentais dos quatro experimentos foram o inteiramente casualizado, com 10 tratamentos e 5 repetições, em fatorial 3x3+1, considerando-se o contraste entre mesotrione (96 g ha-1) e  herbicidas inibidores do Fotossistema II (ametryn, 1500 g ha-1; metribuzin, 1440 g ha-1; diuron + hexazinone, 936+264 g ha-1 e atrazine, 2000 g ha-1), isolados e em mistura para três condições de absorção e uma testemunha sem aplicação. As condições de absorção constituíram-se pelo isolamento e/ou exposição parcial das plântulas alvo de D. horizontalis (3 a 6 folhas) em relação a deposição das pulverizações pelas modalidades: (a) plântulas protegidas com canudos plásticos em solo descoberto (absorção radicular); (b) plântulas desprotegidas em solo coberto com papel alumínio (absorção foliar); (c) plântulas e solo submetido à deposição dos herbicidas (absorção raízes + folhas) e (d) testemunha. Os maiores níveis de sinergismo obtidos no controle de D. horizontalis foram registrados para a absorção radicular e foliar+radicular da mistura mesotrione + atrazine, assim como da absorção foliar e foliar+radicular de mesotrione + diuron + hexazinone dos 3 aos 7 DAA (dias após aplicação) e de mesotrione + ametryn a partir dos 7 DAA, ambos caracterizados pela redução do tempo inicial do controle em atingir níveis ótimos (≥ 90%). Não foram constatados efeitos antagônicos para as misturas.With the objective of evaluating the potencialization efficiency of mesotrione in tank mixture with inhibitors Photosystem II in Digitaria horizontalis control, four experiments were conducted in plastic greenhouses belonging to Superior School of Agronomy of Paraguaçu Paulista County, São Paulo State, being used a Latosoil with sandy texture. The experimental design of four experiments was entirely randomized, with 10 treatments and 5 replications, in factorial scheme 3x3+1, being considered the contrast between mesotrione (96 g ha-1) and inhibitors of the Photosystem II herbicide (ametryn, 1500 g ha-1; metribuzin, 1440 g ha-1; diuron + hexazinone, 936+264 g ha-1 and atrazine, 2000 g ha-1) products, isolated and in tank mixture for three absorption conditions and a check. The absorption conditions were constituted by the isolation and/or partial exhibition of target seedling of D. horizontalis (3 to 6 leaves) in relation to deposition of pulverizations for the modalities: a) seedlings protected with plastic tubes in discovered soil (root absorption pathway); (b) unprotected seedlings in soil covered with aluminum sheet (leaf absorption pathway); (c) seedlings and soil submitted to deposition of the herbicides (absorption roots + leaves pathways) and (d) check. The largest synergism levels obtained for D. horizontalis control were registered to root absorption and leaf + root of the tank mixture with mesotrione + atrazine, as well as for leaf absorption and leaf + root of mesotrione + diuron + hexazinone from the 3 to the 7 DAA (days after application) and of mesotrione + ametryn starting from 7 DAA, both characterized by reduction of control initial time in reaching great levels (≥ 90%). Antagonistic effects were not verified for the tank mixtures

Correlations between Risk Factors for Breast Cancer and Genetic Instability in Cancer Patients- A Clinical Perspective Study

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.Molecular epidemiological studies have identified several risk factors linking to the genes and external factors in the pathogenesis of breast cancer. In this sense, genetic instability caused by DNA damage and DNA repair inefficiencies are important molecular events for the diagnosis and prognosis of therapies. Therefore, the objective of this study was to analyze correlation between sociocultural, occupational, and lifestyle risk factors with levels of genetic instability in non-neoplastic cells of breast cancer patients. Total 150 individuals were included in the study that included 50 breast cancer patients submitted to chemotherapy (QT), 50 breast cancer patients submitted to radiotherapy (RT), and 50 healthy women without any cancer. Cytogenetic biomarkers for apoptosis and DNA damage were evaluated in samples of buccal epithelial and peripheral blood cells through micronuclei and comet assay tests. Elder age patients (61–80 years) had higher levels of apoptosis (catriolysis by karyolysis) and DNA damage at the diagnosis (baseline damage) with increased cell damage during QT and especially during RT. We also reported the increased frequencies of cytogenetic biomarkers in patients who were exposed to ionizing radiation as well as for alcoholism and smoking. QT and RT induced high levels of fragmentation (karyorrhexis) and nuclear dissolution (karyolysis) and DNA damage. Correlations were observed between age and karyorrhexis at diagnosis; smoking and karyolysis during RT; and radiation and karyolysis during QT. These correlations indicate that risk factors may also influence the genetic instability in non-neoplastic cells caused to the patients during cancer therapies

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

Em torno da mesa do rei: artefatos, convivialidade e celebração no Rio de Janeiro joanino

Este artigo aborda um aspecto relevante do ritual das refeições na corte de D. João VI, no Rio de Janeiro: a prataria de mesa. Isto é, objetos de luxo destinados a servir e a consumir os alimentos. Com base em um inventário de bens de mesa enviados do Rio de Janeiro para Lisboa junto com o monarca em seu retorno a Portugal, em 1821, este artigo procura refletir sobre as funções e possíveis usos desses objetos, assim como sobre a importância desse universo material para o funcionamento, representação e celebração da Casa Real portuguesa na sua nova sede. O artigo argumenta que a presença e o uso de baixelas de prata de serviço, mas também de grande aparato, nas refeições públicas da família real pode contribuir para se repensar a imagem de uma corte pobre e sem brilho recorrentemente reforçada na historiografia do período joanino.This article discusses a specific issue of the royal dinner's rituals at the Court of Rio de Janeiro: the silver service: some luxury artifacts of different forms specially made to serve and consume food. Based on a inventory of these silver objects and also on a stock of table linen which were shipped to Lisbon, along with the royal family in 1821, The article intend to explore the functions and utilities of those objects at the table, as well as, analyze the importance of this material universe for the functioning, representation and celebration of the Portuguese Royal House in its new headquarters.The article argues that the use and even the exhibition of the banquet silver in public ceremonies may contribute to change a rather depreciative image of poverty the royal court in Brazil, which has being explored by the historiography for more than a hundred years