456 research outputs found
Amyotrophic lateral sclerosis Prospective study on respiratory parameters
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Objective: To verify how efficient respiratory parameters are in the follow-up of subjects with amyotrophic lateral sclerosis (ALS) and to observe possible correlations between respiratory and nutritional functions. Method: Sixteen patients with probable or defined ALS were selected and evaluated over eight months using the following respiratory parameters: spirometry, maximum inspiratory pressure (MIP), maximum expiratory pressure (MEP), arterial gasometry and pulse oximetry; and nutritional parameters such as body mass index (BMI) and percentage weight loss. Results: P(a)CO(2) was a significant parameter to follow up disease evolution (p=0.051). There was significant correlation between MIP and MEP (r: 0.83); BMI and MIP (r: 0.70); BMI and MEP (r: 0.72); pulse oximetry and forced vital capacity (r: 0.57). Conclusion: P(a)CO(2) was shown to be an efficient and significant parameter in the measurement of respiratory impairment; the correlations among MIP, MEP and BMI indicated that these are significant parameters for periodic clinical evaluation.682258262Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES
DXA assessment
Accurate assessment of body composition is an important issue among athletes. Different methodologies generate controversial results, leading to a deep uncertainty on individual exercise prescriptions. Thus, this study aims to identify the differences between field methods, such as bioelectrical impedance (BIA) and skinfold assessment, with a clinical method, highly accurate, dual-energy X-ray absorptiometry (DXA), among elite young football players. Thirty-eight male football players with a mean (sd) age of 16.7 (0.87) years, involved in the Portuguese national competition of U16 (n = 13) and U19 (n = 25), were evaluated and objective measures of body composition, muscle strength and football skills were collected by trained specialists. Body composition was assessed using BIA (Tanita BC-418, Tanita Corp., Tokyo, Japan), in agreement with all the evaluation premises. Additionally, all athletes were evaluated using the clinical method
DXA (Hologic Inc., Waltham, MA, USA). Among the U19 athletes, three skinfold sites (SKF) were assessed: chest, abdomin and thigh. The Spearman correlation coefficients and the mean difference between methods were calculated. The agreement between both methods was analyzed using Bland-Altman plots. Among the evaluated athletes, lower mean values of body fat % were found using BIA as a method of body composition assessment compared with DXA (12.05 vs. 15.58 for U16; 11.97 vs. 14.16 for U19). Despite the moderate correlation between methods (r = 0.33) to estimate the percentage of total fat, the median of the difference (DXA vs. BIA) was relevant in clinical terms, with 2.90% and 1.47% for U16 and U19 athletes, respectively. Stronger correlations were found between the sum of the SKF and DXA fat estimation (r = 0.68). The Bland-Altman plots showed a clear underestimation in the evaluations using the BIA, namely among athletes with better body composition profiles (8%?12% of fat). Using BIA, an underestimation of body fat assessment was
observed among 94.5% of the athletes with less than 12% body fat mass. Among the evaluated athletes, fat mass was underestimated at a median value of 2.21% using BIA in comparison with DXA. The sum of the SKF showed a stronger correlation with the reference method (DXA) (r = 0.68) than BIA.D915-7373-ED16 | Cesar LeaoN/
effects of the pre-season period
Study aim: To assess changes in physical fitness of amateur soccer players after a pre-season training period and baseline fitness dependencies.
Material and methods: Twenty-one amateur soccer players were assessed during the pre-season. The following physical vari ables were assessed before and after a two-month pre-season training period: (i) cardiorespiratory fitness, (ii) strength and power, and (iii) change of direction (COD).
Results: Significant decreases were found for countermovement jump (CMJ) (p < 0.001; d = 1.161), drop jump (DJ) (p = 0.014; d = 0.958), and horizontal jump (HJ) (p = 0.042; d = 0.640), while no significant changes were found for the overall variables from the beginning to the end of pre-season. Fit players revealed significant decreases for CMJ (p = 0.002; d = ?2.495), DJ (p = 0.004; d = ?1.760), HJ (p = 0.028; d = ?1.005), COD deficit (p = 0.034; d = 1.013), and maximal aerobic speed (MAS) (p = 0.026; d = ?4.053). No significant changes were found for unfit players.
Conclusions: Amateur soccer coaches should consider assessing physical qualities at the beginning of pre-season and use the free-of-charge monitoring tools such as session-rate of perceived exertion (s-RPE) during the training process.D915-7373-ED16 | Cesar LeaoN/
Metal transfer to sediments, invertebrates and fish following waterborne exposure to silver nitrate or silver sulfide nanoparticles in an indoor stream mesocosm.
The fate of engineered nanomaterials in ecosystems is unclear. An aquatic stream mesocosm was explored the fate and bioaccumulation of silver sulfide nanoparticles (Ag2S NPs) compared to silver nitrate (AgNO3). The aims were to determine the total Ag in water, sediment and biota, and to evaluate the bioavailable fractions of silver in the sediment using a serial extraction method. The total Ag in the water column from a nominal daily dose of 10 μg L-1 of Ag for the AgNO3 or Ag2S NP treatments reached a plateau of around 13 and 12 μg L-1, respectively, by the end of the study. Similarly, the sediment of both Ag-treatments reached ~380 μg Ag kg-1, and with most of it being acid-extractable/labile. The biota accumulated 4-59 μg Ag g-1 dw, depending on the type of Ag-treatment and organism. The oligochaete worm, Lumbriculus variegatus, accumulated Ag from the Ag2S exposure over time, which was similar to the AgNO3 treatment by the end of the experiment. The planarian, Girardia tigrina, and the chironomid larva, Chironomus riparius, showed much higher Ag concentrations than the oligochaete worms; and with a clearer time-dependent statistically significant Ag accumulation relative to the untreated controls. For the pulmonated snail, Physa acuta, bioaccumulation of Ag from AgNO3 and Ag2S NP exposures was observed, but was lower from the nano treatment. The AgNO3 exposure caused appreciable Ag accumulation in the water flea, Daphnia magna, but accumulation was higher in the Ag2S NP treatment (reaching 59 μg g-1 dw). In the rainbow trout, Oncorhynchus mykiss, AgNO3, but not Ag2S NPs, caused total Ag concentrations to increase in the tissues. Overall, the study showed transfer of total Ag from the water column to the sediment, and Ag bioaccumulation in the biota, with Ag from Ag2S NP exposure generally being less bioavailable than that from AgNO3
Rapid viral metagenomics using SMART-9N amplification and nanopore sequencing [version 2; peer review: 2 approved]
Emerging and re-emerging viruses are a global health concern. Genome sequencing as an approach for monitoring circulating viruses is currently hampered by complex and expensive methods. Untargeted, metagenomic nanopore sequencing can provide genomic information to identify pathogens, prepare for or even prevent outbreaks. SMART (Switching Mechanism at the 5' end of RNA Template) is a popular approach for RNA-Seq but most current methods rely on oligo-dT priming to target polyadenylated mRNA molecules. We have developed two random primed SMART-Seq approaches, a sequencing agnostic approach 'SMART-9N' and a version compatible rapid adapters available from Oxford Nanopore Technologies 'Rapid SMART-9N'. The methods were developed using viral isolates, clinical samples, and compared to a gold-standard amplicon-based method. From a Zika virus isolate the SMART-9N approach recovered 10kb of the 10.8kb RNA genome in a single nanopore read. We also obtained full genome coverage at a high depth coverage using the Rapid SMART-9N, which takes only 10 minutes and costs up to 45% less than other methods. We found the limits of detection of these methods to be 6 focus forming units (FFU)/mL with 99.02% and 87.58% genome coverage for SMART-9N and Rapid SMART-9N respectively. Yellow fever virus plasma samples and SARS-CoV-2 nasopharyngeal samples previously confirmed by RT-qPCR with a broad range of Ct-values were selected for validation. Both methods produced greater genome coverage when compared to the multiplex PCR approach and we obtained the longest single read of this study (18.5 kb) with a SARS-CoV-2 clinical sample, 60% of the virus genome using the Rapid SMART-9N method. This work demonstrates that SMART-9N and Rapid SMART-9N are sensitive, low input, and long-read compatible alternatives for RNA virus detection and genome sequencing and Rapid SMART-9N improves the cost, time, and complexity of laboratory work
Factores que inhiben la decisión de estudiar un programa de posgrado modalidad virtual en Lima Metropolitana
Durante el año 2020 y producto de la pandemia de la COVID-19, las clases en los distintos niveles educativos migraron intempestivamente de la modalidad presencial a la modalidad virtual con el objetivo de que las personas de todas las edades no detuvieran sus estudios. En lo concerniente a la educación posgrado, miles de profesionales tuvieron la oportunidad de experimentar por primera vez las características propias de la educación en línea, la misma que si bien hasta antes de la pandemia venía creciendo en el Perú y el mundo, tenía ciertas limitaciones y reparos de nuestro país. En esta línea, incluso la misma legislación de la Superintendencia Nacional de Educación Superior Universitaria del Perú, no permitía que existiesen programas de maestría y doctorados 100% virtuales hasta antes de la pandemia. Frente a esta situación, la presente investigación tiene como objetivo general determinar qué factores, tanto personales como sociales, inhiben la decisión de estudiar un programa de posgrado modalidad virtual en Lima Metropolitana. El propósito principal es brindar información valiosa sobre las razones que provocan que una persona no desee estudiar de manera virtual aun cuando vivimos en una sociedad cada vez más digitalizada
EVALUATION OF FOUR DIFFERENT DNA EXTRACTION METHODS IN COAGULASE-NEGATIVE STAPHYLOCOCCI CLINICAL ISOLATES
Studies of micronuclei and other nuclear abnormalities in red blood cells of Colossoma macropomum exposed to methylmercury
The frequencies of micronuclei (MN) and morphological nuclear abnormalities (NA) in erythrocytes in the peripheral blood of tambaqui (Colossoma macropomum), treated with 2 mg.L−1 methylmercury (MeHg), were analyzed. Two groups (nine specimens in each) were exposed to MeHg for different periods (group A - 24 h; group B - 120 h). A third group served as negative control (group C, untreated; n = 9). Although, when compared to the control group there were no significant differences in MN frequency in the treated groups, for NA, the differences between the frequencies of group B (treated for 120 h) and the control group were extremely significant (p < 0.02), thus demonstrating the potentially adverse effects of MeHg on C. macropomum erythrocytes after prolonged exposure
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