Helicobacter pylori and Epstein-Barr virus infection in gastric diseases: Correlation with IL-10 and IL1RN polymorphism.

Introduction: Helicobacter pylori and Epstein-Barr virus (EBV) infection have recently 23 been shown to be associated with gastric diseases. Polymorphisms in genes encoding 24 cytokines such as interleukin 10 (IL-10) and interleukin 1 Receptor (IL-1RN) influence 25 cytokine secretion levels and appear to contribute to the risk of developing gastroduodenal 26 diseases. 27 To our knowledge, this is the first preliminary study to address the association of 28 coinfection with H. pylori and EBV and their correlation with genetic predisposition in the 29 development of gastric diseases. 30 Methods: Gastric biopsy samples of 96 patients with different gastric diseases were used. 31 Results: Our results showed that the rate of co-infection was higher in patients with 32 gastric cancer than in patients with normal gastric mucosa, active chronic gastritis and 33 MALT lymphoma. As regards the characterization of H. pilory strains, the 34 polymorphism s1m1i1 of vacA gene was more frequent in patients with MALT 35 Lymphoma in comparison to others, while the polymorphism s2m2i2 was most 36 frequent in patients with normal gastric mucosa. In addition, patients who tested 37 positivefor the cagA gene were more frequently those affected with gastric cancer than 38 those with inactive chronic gastritis. Similarly, the patients with oipA gene ON were more 39 frequently those with gastric cancer than those with inactive chronic gastritis. 40 Conclusion: According to our analysis, there was no correlation between coinfection 41 and polymorphisms in genes encoding IL-10 and IL-1RN. We conclude that various 42 factors can be involved in the development of gastric diseases

Knowledge and adherence to the Mediterranean diet in individuals practicing regular amatorial physical activity: a cross-sectional study conducted in the Metropolitan Area of Palermo, Italy

IntroductionMediterranean Diet (MD) is a universal model of nutrition that prevents several metabolic, cardiovascular, and oncological diseases. Main objective of the present study was to analyze adherence and knowledge regarding MD principles in a sample of individuals practicing amatorial sports from the Metropolitan Area of Palermo. MethodsA cross-sectional study was conducted in 10 Sports Centers, between October 2020 and September 2021, through a previously validated anonymous questionnaire structured in five sections including 74 items. ResultsOverall, 337 subjects answered to the questionnaire. Based on the multivariable analysis conducted, a higher knowledge score (KS) on MD principles was observed among individuals daily consuming vegetables (OR: 3.32; CI95%: 1.82-6.02) and in the ones more adherent to MD principles (OR: 10.15; CI95%:5.47-18.85). More in depth, using MEDAS score to analyze the adherence to MD, a significant lower adherence was observed among overweight/obese (OR: 0.57; CI95%:0.33-0.99) and among employed subjects (OR: 0.52; IC95%: 0.28-0.98); while, a higher adherence was highlighted among daily consumers of vegetables (OR: 2.52; CI95%:1.52-4.17), daily consumers of fruit (OR: 1.77; CI95%:1.08-2.90), and in individuals that have daily breakfast (OR: 4.29; CI95%:1.15-15.96). DiscussionIn accordance with the WHO Europe Gaining Health Campaign, Public Health Authorities should simplify accessibility to healthy food among general population, promoting principles and accessibility to MD

Implant replacement and anaplastic large cell lymphoma associated with breast implants: a quantitative analysis

Breast implant-associated anaplastic large-cell lymphoma (BIAALCL) is a rare form of non-Hodgkin T-cell lymphoma associated with breast reconstruction post-mastectomy or cosmetic-additive mammoplasty. The increasing use of implants for cosmetic purposes is expected to lead to an increase in BIA-ALCL cases. This study investigated the main characteristics of the disease and the factors predicting BIA-ALCL onset in patients with and without an implant replacement

Biological Response to Bioinspired Microporous 3D-Printed Scaffolds for Bone Tissue Engineering

The scaffold is a key element in the field of tissue engineering, especially when large defects or substitutions of pathological tissues or organs need to be clinically addressed. The expected outcome is strongly dependent on the cell–scaffold interaction and the integration with the surrounding biological tissue. Indeed, mimicking the natural extracellular matrix (ECM) of the tissue to be healed represents a further optimization that can limit a possible morphological mismatch between the scaffold and the tissue itself. For this aim, and referring to bone tissue engineering, polylactic acid (PLA) scaffolds were 3D printed with a microstructure inspired by the trabecular architecture and biologically evaluated by means of human osteosarcoma SAOS-2 cells. The cells were seeded on two types of scaffolds differing for the designed pore size (i.e., 400 and 600 µm), showing the same growth exponential trend found in the control and no significant alterations in the actin distribution. The microporous structure of the two tested samples enhanced the protein adsorption capability and mRNA expression of markers related to protein synthesis, proliferation, and osteoblast differentiation. Our findings demonstrate that 3D-printed scaffolds support the adhesion, growth, and differentiation of osteoblast-like cells and the microporous architecture, mimicking the natural bone hierarchical structure, and favoring greater bioactivity. These bioinspired scaffolds represent an interesting new tool for bone tissue engineering and regenerative medicine applications

Chiral Cyclobutane-Containing Cell-Penetrating Peptides as Selective Vectors for Anti-Leishmania Drug Delivery Systems

Altres ajuts: CSIC (PIE 201620E038)Two series of new hybrid γ/γ-peptides, γ-CC and γ-CT, formed by (1S,2R)-3-amino2,2,dimethylcyclobutane-1-carboxylic acid joined in alternation to a Nα- functionalized cis- or trans-γ-amino-l-proline derivative, respectively, have been synthesized and evaluated as cell penetrating peptides (CPP) and as selective vectors for anti-Leishmania drug delivery systems (DDS). They lacked cytotoxicity on the tumoral human cell line HeLa with a moderate cell-uptake on these cells. In contrast, both γ-CC and γ-CT tetradecamers were microbicidal on the protozoan parasite Leishmania beyond 25 µM, with significant intracellular accumulation. They were conjugated to fluorescent doxorubicin (Dox) as a standard drug showing toxicity beyond 1 µM, while free Dox was not toxic. Intracellular accumulation was 2.5 higher than with Dox-TAT conjugate (TAT = transactivator of transcription, taken as a standard CPP). The conformational structure of the conjugates was approached both by circular dichroism spectroscopy and molecular dynamics simulations. Altogether, computational calculations predict that the drug-γ-peptide conjugates adopt conformations that bury the Dox moiety into a cavity of the folded peptide, while the positively charged guanidinium groups face the solvent. The favorable charge/hydrophobicity balance in these CPP improves the solubility of Dox in aqueous media, as well as translocation across cell membranes, making them promising candidates for DDS

The Hot and Energetic Universe: A White Paper presenting the science theme motivating the Athena+ mission

This White Paper, submitted to the recent ESA call for science themes to define its future large missions, advocates the need for a transformational leap in our understanding of two key questions in astrophysics: 1) How does ordinary matter assemble into the large scale structures that we see today? 2) How do black holes grow and shape the Universe? Hot gas in clusters, groups and the intergalactic medium dominates the baryonic content of the local Universe. To understand the astrophysical processes responsible for the formation and assembly of these large structures, it is necessary to measure their physical properties and evolution. This requires spatially resolved X-ray spectroscopy with a factor 10 increase in both telescope throughput and spatial resolving power compared to currently planned facilities. Feedback from supermassive black holes is an essential ingredient in this process and in most galaxy evolution models, but it is not well understood. X-ray observations can uniquely reveal the mechanisms launching winds close to black holes and determine the coupling of the energy and matter flows on larger scales. Due to the effects of feedback, a complete understanding of galaxy evolution requires knowledge of the obscured growth of supermassive black holes through cosmic time, out to the redshifts where the first galaxies form. X-ray emission is the most reliable way to reveal accreting black holes, but deep survey speed must improve by a factor ~100 over current facilities to perform a full census into the early Universe. The Advanced Telescope for High Energy Astrophysics (Athena+) mission provides the necessary performance (e.g. angular resolution, spectral resolution, survey grasp) to address these questions and revolutionize our understanding of the Hot and Energetic Universe. These capabilities will also provide a powerful observatory to be used in all areas of astrophysics

The Hot and Energetic Universe: A White Paper presenting the science theme motivating the Athena+ mission

This White Paper, submitted to the recent ESA call for science themes to define its future large missions, advocates the need for a transformational leap in our understanding of two key questions in astrophysics: 1) How does ordinary matter assemble into the large scale structures that we see today? 2) How do black holes grow and shape the Universe? Hot gas in clusters, groups and the intergalactic medium dominates the baryonic content of the local Universe. To understand the astrophysical processes responsible for the formation and assembly of these large structures, it is necessary to measure their physical properties and evolution. This requires spatially resolved X-ray spectroscopy with a factor 10 increase in both telescope throughput and spatial resolving power compared to currently planned facilities. Feedback from supermassive black holes is an essential ingredient in this process and in most galaxy evolution models, but it is not well understood. X-ray observations can uniquely reveal the mechanisms launching winds close to black holes and determine the coupling of the energy and matter flows on larger scales. Due to the effects of feedback, a complete understanding of galaxy evolution requires knowledge of the obscured growth of supermassive black holes through cosmic time, out to the redshifts where the first galaxies form. X-ray emission is the most reliable way to reveal accreting black holes, but deep survey speed must improve by a factor ~100 over current facilities to perform a full census into the early Universe. The Advanced Telescope for High Energy Astrophysics (Athena+) mission provides the necessary performance (e.g. angular resolution, spectral resolution, survey grasp) to address these questions and revolutionize our understanding of the Hot and Energetic Universe. These capabilities will also provide a powerful observatory to be used in all areas of astrophysics