Cis-trimethoxystilbene, exhibits higher genotoxic and antiproliferative effects than its isomer trans-trimethoxystilbene in mcf-7 and mcf-10a cell lines

Stilbenes are a class of natural compounds with a wide variety of biological effects, such as antitumor activity. The best-known stilbene is resveratrol, whose clinical application is limited due to its low bioavailability. Methoxylated derivatives of this stilbene, including cis-trimethoxystilbene (cis-TMS) and trans-trimethoxystilbene (trans-TMS) have demonstrated more pronounced cytotoxic and anti-proliferative effects than resveratrol. Thus, the objective of this study is to evaluate and compare the cytotoxicity and antiproliferative effects of cis-and trans-TMS in MCF-7 and its normal counterpart MCF-10A. Both compounds were cytotoxic, genotoxic, and induced G2-M accumulation and cell death in the two cell lines. These results suggested that the genotoxicity of cis-and trans-TMS is involved in the reduction of cellular proliferation of MCF-7 and MCF-10A cells, but notably, such antiproliferative effects are more pronounced for cis-than trans-TMS

Avaliação citotóxica, genotóxica e antiprofilerativa de Cinchona officinalis L. (Rubiaceae) / Cytotoxic, genotoxic and anti-prophylactic evaluation of Cinchona officinalis L. (Rubiaceae)

A espécie Cinchona officinalis L. pertence a ordem Rubiales e à família Rubiaceae é popularmente conhecida como China, Quina ou Quininha, possui alto valor econômico e medicinal, sendo muito utilizada para tratar doenças como a malária, cãibras musculares, inflamações entre outras. O objetivo deste trabalho foi avaliar a padronização correta das diferentes concentrações de C. officinalis utilizando dois bioindicadores como teste in vivo Allium cepa e Pisum sativum. Os meristemas radiculares de Allium cepa e Pisum sativum após obterem desenvolvimento de 2 mm das radículas em placa de petri com papel germitest e água destilada e, no caso de A. cepa com raízes de 2 cm em copos descartáveis obtidas sob água destilada, foram expostos aos diferentes tratamentos das infusões. Foram utilizadas 3 concentrações (1g, 2g e 3g) da casca de C. officinalis para a preparação da infusão, e os controles negativo (água destilada) e positivo (paracetamol 20%). Os meristemas foram coletados após 24, 48, 72 e 96 horas. Após coleta, os meristemas foram lavados em água destilada com 3 trocas consecutivas e fixados em solução de metanol : ácido acético (3:1) por pelo menos 24 horas sob refrigeração. Para análise do material, os meristemas foram submetidos a técnica de esmagamento com uma gota de Orceina acética 2%. Foram realizadas 15 lâminas de cada tratamento e contabilizadas 300 células por lâmina. A observação das lâminas foi realizada em microscópio óptico sob magnitude de 400x, sob a técnica de varredura. Os biotestes A. cepa e P. sativum foram sensíveis e eficientes para analisar a toxidez de C. officinalis, foi verificado efeito antiprofilerativo nos dois bioindicadores, indicando assim a citogenotoxicidade da planta

Influence of mandibular and palatal intraoral appliances on erosion in situ study outcome

The standardization of in situ protocols for dental erosion is important to enable comparison between studies. Objective: Thus, the objectives of this study were to evaluate the influence of the location of in situ intraoral appliance (mandibular X palatal) on the extent of enamel loss induced by erosive challenges and to evaluate the comfort of the appliances. Material and Methods: One hundred and sixty bovine enamel blocks were selected according to their initial surface hardness and randomly divided into two groups: GI - palatal appliance and GII - mandibular appliance. Twenty volunteers wore simultaneously one palatal appliance (containing 4 enamel blocks) and two mandibular appliances (each one containing 2 enamel blocks). Four times per day during 5 days, the volunteers immersed their appliances in 0.01 M hydrochloric acid for 2 minutes, washed and reinserted them into the oral cavity for 2 hours until the next erosive challenge. After the end of the in situ phase, the volunteers answered a questionnaire regarding the comfort of the appliances. The loss of tissue in the enamel blocks was determined profilometrically. Data were statistically analyzed by paired t-test, Chi-square and Fisher's Exact Test (p<0.05). Results: The enamel blocks allocated in palatal appliances (GI) presented significantly higher erosive wear when compared to the blocks fixed in mandibular appliances (GII). The volunteers reported more comfort when using the palatal appliance. Conclusions: Therefore, the palatal appliance is more comfortable and resulted in higher enamel loss compared to the mandibular one

Effect of in situ aspartame mouthwash to prevent intrinsic and extrinsic erosive tooth wear

The aim was to evaluate whether aspartame regular mouthwash prior to erosive challenges with citric or hydrochloric acids would be able to prevent erosive enamel wear. This randomized, single blind in situ study was conducted with 3 crossover phases of 5 days. Polished bovine enamel blocks (n=252) were randomly divided among 6 groups/ 3 phases/ 21 volunteers. The groups under study were: aspartame solution (0.024% of aspartame in deionized water - experimental group), deionized water (negative-control) and stannous-containing solution (Elmex® Erosion Protection Dental Rinse; positive-control); subjected to erosion on citric acid or hydrochloric acid. Four times per day the volunteers rinsed the intraoral appliance with the respective solutions (in situ) prior to immersion of half of the appliance in 0.05M citric acid and the other half in 0.01M hydrochloric acid for 120 seconds (extraoral). The response variable was enamel loss by profilometry. Data were analyzed by ANOVA and Tukey?s test (p<0.05). No difference on enamel loss was found between aspartame solution and deionized water. Stannous-solution resulted in less enamel loss compared to deionized water. Hydrochloric acid resulted in higher enamel loss than citric acid. In this model, aspartame was not able to prevent erosive tooth wear against citric or hydrochloric acids

Avaliação da monensina sódica no desempenho de cordeiros suplementados a pasto

The experiment was conducted to evaluate the effect of the ionophore addition to the concentrate supplement on the 0; 25; 50 and 75 mg of monensin/animal/day on levels, in the performance of sheep. It was used 16 lambs average with age of four months old and average initial weight of 17.85 +- 3.84 kg, the animals were kept in a pasture of Bermuda grass Tifton 85 (Cynodon spp.) in on completely randomized design was used. Pasture accumulation and quality were evaluated. Addition of monensin did not affect average daily weight gain, the best feed conversion and feed efficiency. Supplementation of the sodium monensin concentrated supplement did not favored or better performance of animals

Initial clinical evidence on biperiden as antiepileptogenic after traumatic brain injury—a randomized clinical trial

There is currently no efficacious intervention for preventing post-traumatic epilepsy (PTE). Preclinical studies support the potential use of anticholinergics for this condition. The purpose of this study was to evaluate the effects of biperiden as an intervention for preventing PTE. A randomized, double-blinded clinical trial was conducted at HC/FMUSP between 2018–2022. Adults with acute traumatic brain injury (TBI) were randomly assigned to receive biperiden or placebo, for 10 days. The primary outcome was the incidence of PTE while the secondary outcomes included the frequency of seizures, the frequency of any adverse events and mortality after 24 months. The study was powered at a planned enrolment of 132 patients. The trial began in January 2018 and was halted by researchers on March 2020 (and terminated in December 2022) in the face of the global COVID-19 pandemic. Overall, 123 participants were randomized and 112 contributed with data for modified mITT analysis, being that 61 (49.5%) participants completed the 24-month follow-up consult. Data analysis indicated lack of evidence of biperiden for either, the incidence of post-traumatic epilepsy (2.6, 95%CI, 0.65–10.57; p = 0.170) or the mortality rate (1.57, 95%CI, 0.73–3.38; p = 0.248). The frequency of late post-traumatic seizures was higher for biperiden group (2.03, 95%CI = 0.912–3.1597; p &lt;0.001). The present study suggests that there was insufficient evidence regarding the effect of biperiden in preventing PTE after TBI, which underpins the need for larger studies.Clinical trial registration: ClinicalTrials.gov, identifier: NCT01048138

AVANÇOS NA CIRURGIA DE RECONSTRUÇÃO DE TECIDOS MOLES

A cirurgia de reconstrução de tecidos moles tem avançado significativamente, com melhorias em técnicas e materiais. Este artigo revisa os recentes progressos, destacando o uso de retalhos livres como o anterolateral da coxa (ALT) e o radial do antebraço (RFF). Estudos mostram que o ALT é versátil e possui baixa morbidade no sítio doador, sendo eficaz em reconstruções de cavidade oral e extremidades inferiores. A análise de diversos estudos demonstra melhorias na taxa de sucesso dos procedimentos e redução nas complicações. Este artigo discute técnicas cirúrgicas, critérios de seleção de pacientes e resultados clínicos, proporcionando uma visão abrangente das práticas atuais na reconstrução de tecidos moles.&nbsp

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio