55 research outputs found

    Qualidade física, fisiológica e sanitária de sementes de Schinus terebinthifolius Raddi de diferentes plantas matrizes

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    Native to Brazil, Schinus terebinthifolius Raddi is a species known for the reddish-pink color of its fruits, which have great industrial economy importance, are sold as a condiment and also used for the recovery of degraded areas. This species can be sexually propagated, where studies regarding the seed quality to know its agronomic behavior are necessary. The present work was developed in the Laboratórios de Análise de Sementes and Fitopatologia of the Centro de Ciencias Agrárias of the Universidade Federal da Paraíba (CCA / UFPB), in Areia - PB, and aimed to evaluate the physical, physiological and sanitary quality of seeds of S. terebinthifolius, from 15 different mother plants located in Pocinhos - PB. Water content, color, one thousand seed weight, germination, vigor (first count, germination speed index (IVG), emergence, emergence speed index (IVE), length, root and shoot dry weight, in addition to a sanity test were evaluated. A completely randomized experimental design was used. The seeds of the mother plants used had low physiological potential and ranged from 2 to 36% among the plants in the same area considered as unsuitable for replanting due to the low vigor of the ir seeds. Potentially pathogenic fungi, characterized as field pathogens (Alternaria sp., Botrytis sp., Chaetomium sp., Cladosporium sp., Colletotrichum sp., Curvularia sp., Nigrospora sp., Pestalotia sp., Phitomices sp. and Phoma sp.) and storage (Aspergillus sp., A.niger, A. flavus, Fusarium sp., Penicillium sp. and Rhizopus sp.) were detected in the seed samples.Nativa do Brasil, Schinus terebinthifolius Raddi é uma espécie conhecida pela coloração rosa-avermelhada de seus frutos, os quais têm grande importância para a economia industrial, sendo comercializados na forma de condimento, assim como para recuperação de áreas degradadas. A sua propagação pode ocorrer sexuadamente, sendo necessário o estudo da qualidade das sementes para o conhecimento de seu comportamento agronômico. O trabalho foi desenvolvido nos Laboratórios de Análise de Sementes e Fitopatologia pertencentes ao Centro de Ciências Agrárias da Universidade Federal da Paraíba (CCA/UFPB), em Areia - PB, com o objetivo de avaliar a qualidade física, fisiológica e sanitária de sementes de S. terebinthifolius, provenientes de 15 diferentes plantas matrizes localizadas no município de Pocinhos - PB. Para tanto foram avaliados o teor de água, coloração, peso de mil sementes, germinação, vigor (primeira contagem, índice de velocidade de germinação (IVG), emergência, índice de velocidade de emergência (IVE), comprimento, massa seca de raízes e parte aérea) e teste de sanidade, cujo delineamento experimental utilizado foi inteiramente ao acaso. As sementes das plantas matrizes utilizadas são de baixo potencial fisiológico, variando de 2 a 36%, entre as plantas de uma mesma área tidas como impróprias para o replantio devido ao baixo vigor de suas sementes. Os fungos potencialmente patogênicos, caracterizados como de campo (Alternariasp., Botrytissp., Chaetomiumsp., Cladosporiumsp.,Colletotrichumsp., Curvulariasp., Nigrosporasp., Pestalotia sp., Phitomicessp.ePhoma sp.) e de armazenamento (Aspergillussp., A.niger, A. flavus, Fusariumsp., Penicilliumsp. eRhizopussp.) foram detectados em suas sementes

    O olhar dos estudantes atendidos pelo comitê de inclusão e acessibilidade da UFPB sobre suas ações.

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    The understanding of disability is still very limited, so people who have some kind of disability or special educational need (NEE) have been suffering restrictions in the course of their life history. In order to evaluate how students with NEE are being assisted by the Inclusion Committee (CIA) of the Federal University of Paraíba (UFPB), and to understand how their actions are being carried out, this research was developed, presented quantitative and qualitative character. To this end, questionnaires were applied to a sample of 28 college students with NEE. The questionnaires were made available on a virtual platform, in which the interviewees were able to fill in te questions without personal identification and with the consent form. After data collection, the results were analyzed in the light of Bardin’s ideas. From the results, we can state that the process of inclusion in the UFPB has been consolidating effective; to ensure access and permanence of students with disabilities and/or NEE in order to provide them with an equal and quality education. However, there are still challenges to be faced for better attendance to university students’NEE.NenhumaO entendimento de deficiência ainda é bastante limitado, dessa maneira as pessoas que apresentam algum tipo de deficiência ou necessidade educativa especial (NEE), vêm sofrendo restrições no trajeto de sua história de vida. Com o intuito de avaliar como estão sendo atendidos os estudantes com NEE pelo Comitê de Inclusão (CIA)da Universidade Federal da Paraíba (UFPB), e compreender como estão sendo suas açõesa presente pesquisa foi desenvolvida, apresentado caráter quanti-qualitativo. Para tanto foram aplicados questionários para uma amostra de 28dos universitários com NEE. Os questionários foram disponibilizados em uma plataforma virtual, no qual os entrevistados puderam preencher as questões sem identificação pessoal e com preenchimento do termo de consentimento. Após coleta dos dados, os resultados foram analisados sob a luz das ideias de Bardin. A partir dos resultados, podemos afirmar que o processo de inclusão na UFPB vem se consolidando, efetivamente; visando garantir o acesso e permanência dos discentes com deficiência e/ou NEE, de forma a lhes proporcionar uma educação igualitária e de qualidade. Todavia, ainda há desafios a serem enfrentados para o melhor atendimento às NEE dos universitários

    ESTRESSE HÍDRICO E SALINO EM DIFERENTES TEMPERATURAS NA GERMINAÇÃO E VIGOR DE SEMENTES DE Phaseolus lunatus L.

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    The Phaseolus lunatus L. species, popularly known as bean, lima bean or bean-to-file is used for human and animal consumption, for providing vegetable protein and decrease the almost exclusive reliance on common beans (Phaseolus vulgaris L.), which may also be used as compost or green cover crop for soil protection. The objective of this study was to evaluate the germination and vigor of the seeds when subjected to water and salt stress at different temperatures. To evaluate the germination simulating water stress condition polyethylene glycol solutions used were 6000 (PEG 6000) in different osmotic potentials of 0.0; -0.1; -0.2; -0.3; -0.4 And -0.5 MPa at temperatures of 20, 25, 30 and 35 ° C. While saline solutions were prepared using as the solute sodium chloride (NaCl) at concentrations of 0.0; 1.5; 3.0; 4.5 and 6.0 dS m-1 at temperatures of 20, 25, 30, 35 and 20-30 ° C. To determine the effect of the treatments evaluated the percentage, first count and germination speed index and the length and seedling dry matter. The PEG 6000 solution at potentials above -0.4 MPa inhibit germination and seed vigor unfeasible Phaseolus lunatus L., better suiting to 25 ° C. Salt stress induced by NaCl to the potential of 1.5 dS m-1, does not affect the germination of seeds of this species performance, and better performance under alternating temperature of 20-30°C.A espécie Phaseolus lunatus L., conhecida popularmente como fava, feijão-fava ou feijão-de-lima é utilizada na alimentação humana e animal, por fornecer proteína vegetal e diminuir a dependência quase exclusiva dos feijões comuns (Phaseolus vulgaris L.), podendo ainda ser utilizado como adubo verde ou cultura de cobertura para proteção do solo. O objetivo deste trabalho foi avaliar a germinação e o vigor de suas sementes quando submetidas ao estresse hídrico e salino em diferentes temperaturas. Para a avaliação da germinação simulando condição de estresse hídrico foram utilizadas soluções de polietilenoglicol 6000 (PEG 6000) nos diferentes potenciais osmóticos de 0,0; -0,1; -0,2; -0,3; -0,4 e -0,5 MPa, nas temperaturas de 20, 25, 30 e 35°C. Enquanto as soluções salinas foram preparadas utilizando-se como soluto o cloreto de sódio (NaCl), nas concentrações de 0,0; 1,5; 3,0; 4,5 e 6,0 dS m-1 , nas temperaturas de 20, 25, 30, 35 e 20-30°C. Para determinação do efeito dos tratamentos avaliou-se a porcentagem, primeira contagem e índice de velocidade de germinação, bem como o comprimento e massa seca de plântulas. As soluções de PEG 6000 em potenciais acima de -0,4 MPa inibem a germinação e inviabilizam o vigor das sementes de Phaseolus lunatus L., se adequando melhor a temperatura de 25°C. O estresse salino induzido por NaCl até o potencial de 1,5 dS m-1, não afeta o desempenho germinativo de sementes dessa espécie, tendo um melhor desempenho quando submetidas a temperatura alternada de 20-30°C

    A new systematization of histological analysis for the diagnosis of Hirschsprung's disease

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    Background: Hirschsprung's Disease (HD) is characterized by intestinal sub-occlusion and the absence of enteric ganglion cells. A rectal biopsy examination is performed to confirm the diagnosis. In a recent study, we demonstrated that the analysis of 60 sections of rectal mucosa and submucosa stained by H&E may ensure a 90% diagnostic accuracy. Although the need to analyze so many sections makes the process of reading the slides more time-consuming, this encouraged us to study their distribution in the healthy rectal submucosa, to simplify the diagnosis. Objectives: To develop a method that facilitates HD diagnosis by studying the distribution of ganglion cells in the submucosal plexus. Methods: Using the calretinin technique, we studied the distribution of plexuses in 60 fragments of rectal submucosa from 19 cadavers. After the study, the reading method created was used for diagnosis in 47 cases of suspected HD, using H&E staining. The accuracy was verified by comparing the results obtained with H&E to those obtained with the acetylcholinesterase technique, the golden standard in our laboratory. Results: The study of submucosal plexus distribution showed that just by examining the submucosal region every 20 µm, approximately, it is possible to locate a ganglionic plexus, and we have already been able to diagnose HD with 93% accuracy. Conclusion: The study of ganglion cell distribution enabled the creation of a simplified method for reading the slides. The method applied achieved good accuracy and it can be used as an alternative method in HD diagnosis

    Following the trail: factors underlying the sudden expansion of the Egyptian mongoose (Herpestes ichneumon) in Portugal

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    Species range-limits are influenced by a combination of several factors. In our study we aimed to unveil the drivers underlying the expansion of the Egyptian mongoose in Portugal, a carnivore that was confined to southern Portugal and largely increased its range during the last three decades. We evaluated the expansion of the species in three periods (1980- 1990, 1990-2000 and 2000-2010), by projecting the presence/absence data of the species in each temporal range and proposed four hypotheses to explain this sudden expansion associated to changes in the barrier effects of human infrastructure and topographic features, and in the availability of suitable areas due to climate change or land use. An exploratory analysis was made using Spearman rank correlation, followed by a hierarchical partitioning analysis to select uncorrelated potential explanatory variables associated with the different hypotheses. We then ran Generalized Linear Models (GLM) for every period for each hypothesis and for every combination of hypotheses. Our main findings suggest that dynamic transitions of land-use coupled with temperature and rainfall variations over the decades are the main drivers promoting the mongoose expansion. The geographic barriers and the human infrastructures functioned as barriers for mongoose expansion and have shaped its distribution. The expansion of the Egyptian mongoose across the Portuguese territory was due to a variety of factors. Our results suggest a rapid shift in species range in response to land-use and climate changes, underlining the close link between species ranges and a changing environment

    Aplastic anemia after pediatric liver transplantation

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    A aplasia de medula é uma das mais raras (<1%) e sérias complicações após o transplante hepático por insuficiência hepática aguda grave viral não A, não B e não C. Esta condição clínica, que acomete simultaneamente o tecido hepático e o hematopoético, foi descrita pela primeira vez em 1987, por Stock, e a fisiopatologia relacionada é uma condição imunomediada, provavelmente secundária à infecção viral desconhecida, e associada a grave prognóstico. A recuperação espontânea da aplasia medular adquirida habitualmente é muito rara e 50%-70% dos pacientes respondem ao tratamento imunossupressor com ciclosporina A (CsA) e glubulina antitimocítica (ATG), mesmo após o transplante hepático. Além do tratamento imunossupressor, outra opção é o transplante de medula óssea (TMO). Apresentamos o caso de uma criança com aplasia medular grave após transplante hepático, por insuficiência hepática aguda grave, que recebeu tratamento imunossupressor com CsA e ATG e evoluiu com recuperação completa das três séries do hemograma.Aplastic anemia (AA) is one of the rarest (<1%) and most serious complications of liver transplantation for fulminant non-A, non-B and non-C hepatitis. It was first described in 1987 by Stock; the mechanism involved is an immunologically mediated condition secondary to an unknown viral infection. The disease is associated with a dismal prognosis. Spontaneous recovery from acquired AA is very rare however some patients (50-70%) recover after immunosuppressive therapy, such as Cyclosporin A (CsA) and Antithymocyte globulin (ATG), even after liver transplantation. Another treatment option is bone marrow transplantation. We report on a child who developed AA following liver transplantation for fulminant viral hepatitis that was treated with intensive immunosuppression including CsA and ATG and achieved complete recovery

    Immediate expression of c-fos and c-jun mRNA in a model of intestinal autotransplantation and ischemia-reperfusion in situ

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    OBJECTIVE: Intestinal ischemia-reperfusion injury occurs in several clinical conditions and after intestinal transplantation. The aim of the present study was to investigate the phenomena of apoptosis and cell proliferation in a previously described intestinal ischemia-reperfusion injury autograft model using immunohistochemical markers. The molecular mechanisms involved in ischemia-reperfusion injury repair were also investigated by measuring the expression of the early activation genes c-fos and c-jun, which induce apoptosis and cell proliferation. MATERIALS AND METHODS: Thirty adult male Wistar rats were subjected to surgery for a previously described ischemia-reperfusion model that preserved the small intestine, the cecum and the ascending colon. Following reperfusion, the cecum was harvested at different time points as a representative segment of the intestine. The rats were allocated to the following four subgroups according to the reperfusion time: subgroup 1: 5 min; subgroup 2: 15 min; subgroup 3: 30 min; and subgroup 4: 60 min. A control group of cecum samples was also collected. The expression of c-fos, c-jun and immunohistochemical markers of cell proliferation and apoptosis (Ki67 and TUNEL, respectively) was studied. RESULTS: The expression of both c-fos and c-jun in the cecum was increased beginning at 5 min after ischemia-reperfusion compared with the control. The expression of c-fos began to increase at 5 min, peaked at 30 min, and exhibited a declining tendency at 60 min after reperfusion. A progressive increase in c-jun expression was observed. Immunohistochemical analyses confirmed these observations. CONCLUSION: The early activation of the c-fos and c-jun genes occurred after intestinal ischemia-reperfusion injury, and these genes can act together to trigger cell proliferation and apoptosis

    LA PANDEMIA DEL COVID-19 COMO AUMENTO DEL ESTRÉS EN EL PROCESO DE TRABAJO EN SALUD EN EL SISTEMA PRISIONERO

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    Introdução: o trabalho das equipes de saúde prisional encontra-se entre os mais deteriorantes, tornando os seus trabalhadores vulneráveis ao estresse. Devido à pandemia da COVID-19, o estresse no processo de trabalho destas equipes potencializou-se. Objetivo: demonstrar como a pandemia da COVID-19 potencializa o estresse no processo de trabalho na ótica da equipe de saúde de uma penitenciária do estado da Bahia. Métodos: estudo qualitativo, descritivo e exploratório realizado com 14 trabalhadores de uma equipe de saúde prisional do estado da Bahia. Utilizou-se a entrevista semiestruturada, cujos dados foram submetidos à análise de conteúdo. O estudo foi aprovado pelo Parecer nº. 4.032.879/2020. Resultados e discussão: o estudo sinaliza a pandemia da COVID-19 como desestabilizadora do processo de trabalho da equipe de saúde prisional por ocasionar sofrimento psíquico frente às más condições de trabalho, falta de treinamento e de equipamento de proteção individual adequados, tornando os seus trabalhadores vulneráveis pelo contato direto com uma população de alto risco. Conclusão: a pandemia da COVID-19 apresenta um cenário marcado por contradições em que as unidades prisionais são consideradas epicentros para doenças infecciosas, o que gera estresse no desenvolvimento do processo de trabalho da equipe de saúde prisional em ambiente de trabalho inseguro e de alta periculosidade, ocasionando impactos na saúde e na satisfação geral dos trabalhadores. Recomenda-se implementar estratégias de enfrentamento ao estresse no contexto da pandemia da COVID-19, para promover qualidade de vida e mitigar o impacto psicossocial nos trabalhadores da equipe de saúde prisional.Introduction: the work of prison health teams is among the most damaging, making their workers vulnerable to stress. Due to the COVID-19 pandemic, the stress in the work process of these teams has been increased. Objective: to demonstrate how the COVID-19 pandemic potentiates stress in the work process from the perspective of the health team of a penitentiary in the state of Bahia. Methods: qualitative, descriptive and exploratory study conducted with 14 workers from a prison health team in the state of Bahia. The semi-structured interview was used, whose data were submitted to content analysis. The study was approved by Opinion nº. 4.032.879/2020. Results and discussion: the study signals the COVID-19 pandemic as destabilizing the prison health team's work process by causing psychological distress in the face of poor working conditions, lack of training and adequate personal protective equipment, making its workers vulnerable by direct contact with a high-risk population. Conclusion: the COVID-19 pandemic presents a scenario marked by contradictions in which prison units are considered epicenters for infectious diseases, which generates stress in the development of the prison health team's work process in an unsafe and highly dangerous work environment, causing impacts on the health and general satisfaction of workers. It is recommended to implement strategies to cope with stress in the context of the COVID-19 pandemic, to promote quality of life and mitigate the psychosocial impact on prison health team workers.Introducción: el trabajo de los equipos de salud penitenciaria es uno de los más perjudiciales, lo que hace que sus trabajadores sean vulnerables al estrés. Debido a la pandemia de COVID-19, se ha incrementado el estrés en el proceso de trabajo de estos equipos. Objetivo: demostrar cómo la pandemia COVID-19 potencia el estrés en el proceso de trabajo desde la perspectiva del equipo de salud de un centro penitenciario del estado de Bahía. Métodos: estudio cualitativo, descriptivo y exploratorio realizado con 14 trabajadores de un equipo de salud penitenciario del estado de Bahía. Se utilizó la entrevista semiestructurada, cuyos datos fueron sometidos a análisis de contenido. El estudio fue aprobado por Opinión nº. 4.032.879/2020. Resultados y discusión: el estudio señala que la pandemia de COVID-19 desestabiliza el proceso de trabajo del equipo de salud penitenciario al generar malestar psicológico ante las malas condiciones laborales, la falta de capacitación y equipo de protección personal adecuado, haciendo vulnerables a sus trabajadores por el contacto directo con una población de alto riesgo. Conclusión: la pandemia COVID-19 presenta un escenario marcado por contradicciones en el que las unidades penitenciarias son consideradas epicentros de enfermedades infecciosas, lo que genera estrés en el desarrollo del proceso de trabajo del equipo de salud en un ambiente laboral inseguro y altamente peligroso, provocando impactos en la salud y satisfacción general de los trabajadores. Se recomienda implementar estrategias para enfrentar el estrés en el contexto de la pandemia COVID-19, promover la calidad de vida y mitigar el impacto psicosocial en los trabajadores del equipo de salud penitenciario
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