Surface Barkhausen Noise Investigations of Stress and Leakage Flux Signals in Line Pipe

Pipelines are subjected to a number of different sources of stress. The principal in-service stress component is due to line pressure, with operating stresses commonly about 60% of the yield strength. Pipelines may also be subjected to considerable bending stresses, particularly when constructed on unstable terrain such as permafrost. Residual stresses may also be present, generally resulting from processing or welding, but more seriously as a consequence of mechanical damage. Anomalously high stress levels, whether residual or applied, may lead to pipeline failure; as a result serious efforts are being made to develop on-line stress detection methods. It is well established that stress is a major factor affecting magnetic properties of ferromagnetic materials, however the effects are complex and have only recently begun to be understood [1,2]. Because of the strong influence of stress on magnetic properties, magnetic NDE techniques are being considered as potential methods for the detection of stress

Phenolic compounds and expression of 4CL genes in silver birch clones and Pt4CL1a lines

A small multigene family encodes 4-coumarate:CoA ligases (4CLs) catalyzing the CoA ligation of hydroxycinnamic acids, a branch point step directing metabolites to a flavonoid or monolignol pathway. In the present study, we examined the effect of antisense Populus tremuloides 4CL (Pt4CL1) to the lignin and soluble phenolic compound composition of silver birch (Betula pendula) Pt4CL1a lines in comparison with non-transgenic silver birch clones. The endogenous expression of silver birch 4CL genes was recorded in the stems and leaves and also in leaves that were mechanically injured. In one of the transgenic Pt4CL1a lines, the ratio of syringyl (S) and guaiacyl (G) lignin units was increased. Moreover, the transcript levels of putative silver birch 4CL gene (Bp4CL1) were reduced and contents of cinnamic acid derivatives altered. In the other two Pt4CL1a lines changes were detected in the level of individual phenolic compounds. However, considerable variation was found in the transcript levels of silver birch 4CLs as well as in the concentration of phenolic compounds among the transgenic lines and non-transgenic clones. Wounding induced the expression of Bp4CL1 and Bp4CL2 in leaves in all clones and transgenic lines, whereas the transcript levels of Bp4CL3 and Bp4CL4 remained unchanged. Moreover, minor changes were detected in the concentrations of phenolic compounds caused by wounding. As an overall trend the wounding decreased the flavonoid content in silver birches and increased the content of soluble condensed tannins. The results indicate that by reducing the Bp4CL1 transcript levels lignin composition could be modified. However, the alterations found among the Pt4CL1a lines and the non-transgenic clones were within the natural variation of silver birches, as shown in the present study by the clonal differences in the transcripts levels of 4CL genes, soluble phenolic compounds and condensed tannins

Multiple plant traits shape the genetic basis of herbivore community assembly

1. Community genetics research has posited a genetic basis to the assembly of ecological communities. For arthropod herbivores in particular, there is strong support that genetic variation in host plants is a key factor shaping their diversity and composition. However, the specific plant phenotypes underlying herbivore responses remain poorly explored for most systems. 2. We address this knowledge gap by examining the influence of both genetic and phenotypic variation in a dominant host-plant species, Salix hookeriana, on its associated arthropod herbivore community in a common garden experiment. Specifically, we surveyed herbivore responses among five different arthropod feeding guilds to 26 distinct S. hookeriana genotypes. Moreover, we quantified the heritability of a suite of plant traits that determine leaf quality (e.g. phenolic compounds, trichomes, specific leaf area, C : N) and whole-plant architecture, to identify which traits best accounted for herbivore community responses to S. hookeriana genotype. 3. We found that total herbivore abundance and community composition differed considerably among S. hookeriana genotypes, with strong and independent responses of several species and feeding guilds driving these patterns. We also found that leaf phenolic chemistry displayed extensive heritable variation, whereas leaf physiology and plant architecture tended to be less heritable. Of these traits, herbivore responses were primarily associated with leaf phenolics and plant architecture; however, different herbivore species and feeding guilds were associated with different sets of traits. Despite our thorough trait survey, plant genotype remained a significant predictor of herbivore responses in most trait association analyses, suggesting that unmeasured host-plant characteristics and/or interspecific interactions were also contributing factors. 4. Taken together, our results support that the genetic basis of herbivore community assembly occurs through a suite of plant traits for different herbivore species and feeding guilds. Still, identifying these phenotypic mechanisms requires measuring a broad range of plant traits and likely further consideration of how these traits affect interspecific interactions.Fil: Barbour, Matthew A.. University Of British Columbia; CanadáFil: Rodriguez Cabal, Mariano Alberto. University Of British Columbia; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue; ArgentinaFil: Wu, Elizabeth T.. Humboldt State University; Estados UnidosFil: Julkunen Tiitto, Riitta. University of Eastern Finland; FinlandiaFil: Ritland, Carol E.. University Of British Columbia; CanadáFil: Miscampbell, Allyson E.. University Of British Columbia; CanadáFil: Jules, Erik S.. Humboldt State University; Estados UnidosFil: Crutsinger, Gregory M.. University Of British Columbia; Canad

Domain wall dynamics and Barkhausen effect in metallic ferromagnetic materials. II. Experiments

Barkhausen effect (BE) phenomenology in iron‐based ferromagnetic alloys is investigated by a proper experimental method, in which BE experiments are restricted to the central part of the hysteresis loop, and the amplitude probability distribution, P0(Φ), and power spectrum, F(ω), of the B flux rate Φ are measured under controlled values of the magnetization rate and differential permeability μ. It is found that all of the experimental data are approximately consistent with the law P0(Φ)∝Φ−1 exp(−Φ/〈Φ〉), where all dependencies on and μ are described by the single dimensionless parameter >0. Also the parameters describing the shape of F(ω) are found to obey remarkably simple and general laws of dependence on and μ. The experimental results are interpreted by means of the Langevin theory of domain‐wall dynamics proposed in a companion paper. The theory is in good agreement with experiments, and permits one to reduce the basic aspects of BE phenomenology to the behavior of two parameters describing the stochastic fluctuations of the local coercive field experienced by a moving domain wall

Biaxial Stress Effects on Barkhausen Signals in a Steel Pipe for the Case of Magnetic Field Noncoaxial with Stress Axes

Many mechanical stress situations in which NDE might be performed are biaxial in character. Examples are gas and oil pipelines, power plant steam pipes, railroad wheels, and turbine blades.</p

Effect of Different Stages of Tensile Deformation on Magnetic Barkhausen Emission in High Strength Low Alloy Steel

Micromagnetic techniques have been considered as a potential non-destructive evaluation (NDE) method for microstructural characterization and stress/strain measurements in ferritic steels [1–14]. The effect of tensile deformation on micromagnetic parameters has been studied by many researchers [8–14]. Most of these studies have been done only in the elastic region and the obtained relation between magnetic parameters and the applied stress has been used to determine the residual stresses, considering that the residual stress level will not exceed the yield stress of the material. These studies have not taken into account the microstructural changes due to dislocations generation by the plastic deformation.</p

Increased Resistance of Bt Aspens to Phratora vitellinae (Coleoptera) Leads to Increased Plant Growth under Experimental Conditions

One main aim with genetic modification (GM) of trees is to produce plants that are resistant to various types of pests. The effectiveness of GM-introduced toxins against specific pest species on trees has been shown in the laboratory. However, few attempts have been made to determine if the production of these toxins and reduced herbivory will translate into increased tree productivity. We established an experiment with two lines of potted aspens (Populus tremula×Populus tremuloides) which express Bt (Bacillus thuringiensis) toxins and the isogenic wildtype (Wt) in the lab. The goal was to explore how experimentally controlled levels of a targeted leaf beetle Phratora vitellinae (Coleoptera; Chrysomelidae) influenced leaf damage severity, leaf beetle performance and the growth of aspen. Four patterns emerged. Firstly, we found clear evidence that Bt toxins reduce leaf damage. The damage on the Bt lines was significantly lower than for the Wt line in high and low herbivory treatment, respectively. Secondly, Bt toxins had a significant negative effect on leaf beetle survival. Thirdly, the significant decrease in height of the Wt line with increasing herbivory and the relative increase in height of one of the Bt lines compared with the Wt line in the presence of herbivores suggest that this also might translate into increased biomass production of Bt trees. This realized benefit was context-dependent and is likely to be manifested only if herbivore pressure is sufficiently high. However, these herbivore induced patterns did not translate into significant affect on biomass, instead one Bt line overall produced less biomass than the Wt. Fourthly, compiled results suggest that the growth reduction in one Bt line as indicated here is likely due to events in the transformation process and that a hypothesized cost of producing Bt toxins is of subordinate significance

Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate.

Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nataša seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of H2O2 and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes.This is a post-peer-review, pre-copyedit version of an article published in Protoplasma. The final authenticated version is available online at: [http://dx.doi.org/10.1007/s00709-018-1250-0