Influencia de la espesura de un pinar albar (Sistema Central, España) en la disponibilidad de luz y agua en el sotobosque, y la supervivencia de robles plantados en su interior

Information of tree-nurse shelterwood effects on survival of underplanted seedlings is particularly scant in Mediterranean forest ecosystems. To study light and water resources availability and survival associated to overstory density, two-year-old seedlings of Quercus petraea (Matt.) Liebl. (sessile oak) and Quercus pyrenaica Willd. (pyrenean oak) were planted in the understory of an even-aged Scots pine (Pinus sylvestris L.) plantation located in central Spain, which had been previously assigned to three density treatments: uncut, 33% thinned and 50% thinned of the original density, each replicated four times. Soil moisture was measured with a TDR during the first growing season after planting. Light conditions were estimated by hemispherical photography. Survival was measured at the end of the first growing season in the field and at the beginning of the next two growing seasons. The reduction in density after thinning had a positive effect on light availability and on near-surface soil moisture. Pyrenean oak had higher survival rates than sessile oak, which showed similarly high mortality rates in all three overstory treatments. Thinning had a positive effect on the survival of pyrenean oak, though irrespective of the intensity. Overall, these results point to the necessity to reduce canopy tree density in Mediterranean mountain pinewoods before carrying out enrichment plantations beneath.Con el fin de estudiar el efecto de la espesura de la cubierta forestal en la disponibilidad de luz y agua para las plantas y su supervivencia, se plantaron brinzales de dos savias de Quercus petraea (Matt.) Liebl. (roble albar) y Quercus pyrenaica Willd. (melojo) en el interior de un pinar de Pinus sylvestris L. (pino albar) situado en el NE de la provincia de Madrid sometido previamente a tres tratamientos: clara del 33% de la densidad original, clara del 50% y ausencia de clara. Se midió la humedad volumétrica del suelo con un TDR y la disponibilidad de luz en el sotobosque por medio de fotografias hemisféricas. La supervivencia se midió al final del primer año y al comienzo del verano de los dos años siguientes. La reducción de la densidad del dosel incrementó la luz disponible en el sotobosque, la humedad de los primeros 10 cm de suelo y la supervivencia de las plantas de melojo, aunque no se apreció un efecto diferente segun la intensidad de la clara aplicada. La mortalidad de las plantas de roble albar fue elevada en todos los tratamientos, y superior a la del melojo. Estos resultados apuntan a la necesidad de reducir la espesura del pinar albar en la zona de estudio antes de llevar a cabo plantaciones con melojo en su interior

Selección de clones de pino piñonero sobresalientes en la producción de piña

Though stone pine is currently a genuine forest tree of Mediterranean ecosystems, its use as grafted orchards crop for its edible pine nuts is potentially feasible. Therefore, genetic improvement of the species has been undertaken by several Spanish forest administrations, establishing in the Nineties experimental clonal orchards by grafting scions from plus trees, selected for their outstanding cone yield in pine forests of the main Spanish provenance regions. This paper analyses the coming into production of four clonal trials located at the National Forest Breeding Centre «Puerta de Hierro», Madrid. The four different-aged orchards showed a strong, synchronized masting habit. Estimations are given for the degree of genetic determination of cone production and for clonal improvement values. Due to the presence of strong size-yield correlations and spatial autocorrelations, genetic parameters were estimated by an individual tree cone-yield model adjusted for environmental tree size differences and for correlations between nearest neighbours (NN). The degree of genetic determination H2, estimated in less than 20% without adjustments, reached 30-38% adjusting for environmental size variation and 45-73% after spatial adjustment, with parallel increments in clonal improvement values and expected genetic gain. The results allow rogueing the four clone banks, progressing in the domestication of the stone pine as nut crop.El potencial uso del pino piñonero como cultivo frutal injertado para la producción de piñón justifica su mejora genética clonal (selección varietal), estableciéndose en los años noventa diversos bancos clonales partiendo de la prospección de árboles sobresalientes en las principales regiones de procedencia españolas. Este trabajo estudia y compara la entrada en producción de cuatro bancos clonales situados en Madrid. Aunque de diferentes edades, muestran un carácter vecero sincronizado entre ellos. Para evitar sesgos ambientales en la estimación del grado de determinación genética de la productividad individual de piña y de los valores de mejora clonales, se ajustaron como covariables las diferencias ambientales de tamaño de árbol y las autocorrelaciones espaciales entre árboles vecinos, que juntos aportaron un coeficiente de determinación mucho mayor que las diferencias entre clones. El grado de determinación genética H2 de la producción individual de piña, estimado en menos de 20% sin ajustar por estas variables ambientales, subió hasta un 30-38% al incluir en el modelo la variación ambiental del tamaño de árbol, hasta un 45-73% tras el ajuste espacial iterativo, incrementándose en paralelo la estimación de los valores de mejora clonal y de la ganancia genética esperada. Estos resultados permiten depurar las colecciones para avanzar en la domesticación de la especie

Ausencia de diferenciación ecotípica entre rodales selectos de pino piñonero en la cuenca del Duero

The relicts of stone pine forests on marly or even gypsiferous slopes with high pH values induced the hypothesis of a possible genetic adaptation to this site types, marginal for a species normally growing on siliceous sandy plains. Actually, the forest reproductive materials from selected seed stands on those slopes are used preferably for forestations on analogous sites. In 1998, a multi-site comparative test was established with plant lots obtained from seven selected seed stands and two non-selected seed sources. At least up to the age of eight years, the saplings did not show any significant differentiation among basic materials in outplanting success, survival, vegetative phase change, height or diameter growth, whereas a common, strong phenotypic plasticity among test sites was observed. Hence there seems to be no reason for managing separately seed lots and plants of the same provenance and certify category (e.g. selected reproductive material) instead of mixing them.La presencia de masas relícticas de pino piñonero sobre cuestas margosas de elevado pH, dentro de la región de procedencia Meseta Norte ha dado lugar a un manejo separado de su material forestal de reproducción por una posible adaptación diferenciada que le conferiría un especial interés para la restauración forestal de estaciones análogas, definiéndose para tal fin unos rodales selectos en las mejores masas sobre cuesta. En 1998, se instaló en esta región un ensayo comparativo de materiales forestales de reproducción de esta región de procedencia de Pinus pinea en varios sitios de ensayo. Tras ocho años, se estudia el comportamiento de los distintos lotes de planta obtenidos de semilla de siete rodales selectos y se compara con dos fuentes de semilla testigo, analizando el arraigo y la supervivencia inicial, el cambio de fase juvenil-adulto y el crecimiento en altura y en diámetro. Contrasta la gran plasticidad fenotípica observada entre sitios de ensayo frente a la diferenciación casi nula entre rodales de origen, al menos en esta fase inicial de establecimiento de la masa. Estos resultados no apoyan el manejo por separado de los materiales de reproducción de una misma categoría, justificándose la mezcla y el manejo común de los lotes de semilla cosechados en los diferentes rodales selectos de la misma región de procedencia

Análisis comparativo de la rentabilidad comercial privada de plantaciones de Pinus pinea L. en tierras agrarias de la provincia de Valladolid

This paper reports an assessment of the economic profitability from the use of grafted stone-pines for afforestations in Valladolid, Central Spain. With this technical change, the plantations begin to yield cones at few years, saving the long unproductive youth stage of traditional stone-pine stands. Some assumptions are made, due to a lack of empirical data, about the expected yield of the grafted plantations in order to obtain their optimal rotation and profitability. As alternative land-use options, the profitability of traditional afforestations and barley crops are calculated. The cases analysed indicate that grafted plantations are competitive under a broad spectrum of essential financial parameter variations, even with the agricultural crop described in this paper. In the basic cases, expected annual return of grafted plantations is about 430-680 euros per hectare, whereas income from barley crop is about 100 euros per hectare.En este trabajo se analiza la rentabilidad económica que supone el uso de plantas injertadas para las forestaciones con Pinus pinea en la provincia de Valladolid. Esta mejora tecnológica permite a las plantaciones entrar en producción de piña en poco tiempo, frente a la dilatada fase de espera en las masas tradicionales. Ante la ausencia de datos empíricos, se han realizado una serie de hipótesis sobre la producción futura de estas masas injertadas. Esta información constituye la piedra angular sobre la que se han calculado el turno óptimo y la rentabilidad de estas plantaciones. Como alternativas, se han estimado las rentabilidades en el caso de que no se adoptara la mejora tecnológica del injerto al reforestar con la especie, o de que se mantuviera el cultivo agrícola tradicional (cebada en secano). Los resultados muestran, ante un amplio espectro de variación de parámetros fundamentales de la inversión, que las plantaciones injertadas resultan plenamente competitivas, incluso con el cultivo agrícola aquí descrito. Bajo las hipótesis base se espera una renta perpetua anual entre 72.000 y 114.000 ptas/ha, frente a 17.000 ptas/ha en el caso de la cebada en secano subvencionada

Accuracy and Prognostic Role of NCCT-ASPECTS Depend on Time from Acute Stroke Symptom-onset for both Human and Machine-learning Based Evaluation.

PURPOSE We hypothesize that the detectability of early ischemic changes on non-contrast computed tomography (NCCT) is limited in hyperacute stroke for both human and machine-learning based evaluation. In short onset-time-to-imaging (OTI), the CT angiography collateral status may identify fast stroke progressors better than early ischemic changes quantified by ASPECTS. METHODS In this retrospective, monocenter study, CT angiography collaterals (Tan score) and ASPECTS on acute and follow-up NCCT were evaluated by two raters. Additionally, a machine-learning algorithm evaluated the ASPECTS scale on the NCCT (e-ASPECTS). In this study 136 patients from 03/2015 to 12/2019 with occlusion of the main segment of the middle cerebral artery, with a defined symptom-onset-time and successful mechanical thrombectomy (MT) (modified treatment in cerebral infarction score mTICI = 2c or 3) were evaluated. RESULTS Agreement between acute and follow-up ASPECTS were found to depend on OTI for both human (Intraclass correlation coefficient, ICC = 0.43 for OTI < 100 min, ICC = 0.57 for OTI 100-200 min, ICC = 0.81 for OTI ≥ 200 min) and machine-learning based ASPECTS evaluation (ICC = 0.24 for OTI < 100 min, ICC = 0.61 for OTI 100-200 min, ICC = 0.63 for OTI ≥ 200 min). The same applied to the interrater reliability. Collaterals were predictors of a favorable clinical outcome especially in hyperacute stroke with OTI < 100 min (collaterals: OR = 5.67 CI = 2.38-17.8, p < 0.001; ASPECTS: OR = 1.44, CI = 0.91-2.65, p = 0.15) while ASPECTS was in prolonged OTI ≥ 200 min (collaterals OR = 4.21,CI = 1.36-21.9, p = 0.03; ASPECTS: OR = 2.85, CI = 1.46-7.46, p = 0.01). CONCLUSION The accuracy and reliability of NCCT-ASPECTS are time dependent for both human and machine-learning based evaluation, indicating reduced detectability of fast stroke progressors by NCCT. In hyperacute stroke, collateral status from CT-angiography may help for a better prognosis on clinical outcome and explain the occurrence of futile recanalization

Correction to: Accuracy and Prognostic Role of NCCT-ASPECTS Depend on Time from Acute Stroke Symptom-onset for both Human and Machine-learning Based Evaluation.

PURPOSE: We hypothesize that the detectability of early ischemic changes on non-contrast computed tomography (NCCT) is limited in hyperacute stroke for both human and machine-learning based evaluation. In short onset-time-to-imaging (OTI), the CT angiography collateral status may identify fast stroke progressors better than early ischemic changes quantified by ASPECTS. METHODS: In this retrospective, monocenter study, CT angiography collaterals (Tan score) and ASPECTS on acute and follow-up NCCT were evaluated by two raters. Additionally, a machine-learning algorithm evaluated the ASPECTS scale on the NCCT (e-ASPECTS). In this study 136 patients from 03/2015 to 12/2019 with occlusion of the main segment of the middle cerebral artery, with a defined symptom-onset-time and successful mechanical thrombectomy (MT) (modified treatment in cerebral infarction score mTICI = 2c or 3) were evaluated. RESULTS: Agreement between acute and follow-up ASPECTS were found to depend on OTI for both human (Intraclass correlation coefficient, ICC = 0.43 for OTI < 100 min, ICC = 0.57 for OTI 100–200 min, ICC = 0.81 for OTI ≥ 200 min) and machine-learning based ASPECTS evaluation (ICC = 0.24 for OTI < 100 min, ICC = 0.61 for OTI 100–200 min, ICC = 0.63 for OTI ≥ 200 min). The same applied to the interrater reliability. Collaterals were predictors of a favorable clinical outcome especially in hyperacute stroke with OTI < 100 min (collaterals: OR = 5.67 CI = 2.38–17.8, p < 0.001; ASPECTS: OR = 1.44, CI = 0.91–2.65, p = 0.15) while ASPECTS was in prolonged OTI ≥ 200 min (collaterals OR = 4.21,CI = 1.36–21.9, p = 0.03; ASPECTS: OR = 2.85, CI = 1.46–7.46, p = 0.01). CONCLUSION: The accuracy and reliability of NCCT-ASPECTS are time dependent for both human and machine-learning based evaluation, indicating reduced detectability of fast stroke progressors by NCCT. In hyperacute stroke, collateral status from CT-angiography may help for a better prognosis on clinical outcome and explain the occurrence of futile recanalization. SUPPLEMENTARY INFORMATION: The online version of this article (10.1007/s00062-021-01110-5) contains supplementary material, which is available to authorized users

Comparing Poor and Favorable Outcome Prediction With Machine Learning After Mechanical Thrombectomy in Acute Ischemic Stroke

Outcome prediction after mechanical thrombectomy (MT) in patients with acute ischemic stroke (AIS) and large vessel occlusion (LVO) is commonly performed by focusing on favorable outcome (modified Rankin Scale, mRS 0–2) after 3 months but poor outcome representing severe disability and mortality (mRS 5 and 6) might be of equal importance for clinical decision-making

Combined Perfusion and Permeability Imaging Reveals Different Pathophysiologic Tissue Responses After Successful Thrombectomy.

Despite successful recanalization of large-vessel occlusions in acute ischemic stroke, individual patients profit to a varying degree. Dynamic susceptibility-weighted perfusion and dynamic T1-weighted contrast-enhanced blood-brain barrier permeability imaging may help to determine secondary stroke injury and predict clinical outcome. We prospectively performed perfusion and permeability imaging in 38 patients within 24 h after successful mechanical thrombectomy of an occlusion of the middle cerebral artery M1 segment. Perfusion alterations were evaluated on cerebral blood flow maps, blood-brain barrier disruption (BBBD) visually and quantitatively on ktrans maps and hemorrhagic transformation on susceptibility-weighted images. Visual BBBD within the DWI lesion corresponded to a median ktrans elevation (IQR) of 0.77 (0.41-1.4) min-1 and was found in all 7 cases of hypoperfusion (100%), in 10 of 16 cases of hyperperfusion (63%), and in only three of 13 cases with unaffected perfusion (23%). BBBD was significantly associated with hemorrhagic transformation (p < 0.001). While BBBD alone was not a predictor of clinical outcome at 3 months (positive predictive value (PPV) = 0.8 [0.56-0.94]), hypoperfusion occurred more often in patients with unfavorable clinical outcome (PPV = 0.43 [0.10-0.82]) compared to hyperperfusion (PPV = 0.93 [0.68-1.0]) or unaffected perfusion (PPV = 1.0 [0.75-1.0]). We show that combined perfusion and permeability imaging reveals distinct infarct signatures after recanalization, indicating the severity of prior ischemic damage. It assists in predicting clinical outcome and may identify patients at risk of stroke progression

Patterns of modern pollen and plant richness across northern Europe

Sedimentary pollen offers excellent opportunities to reconstruct vegetation changes over past millennia. Number of different pollen taxa or pollen richness is used to characterise past plant richness. To improve the interpretation of sedimentary pollen richness, it is essential to understand the relationship between pollen and plant richness in contemporary landscapes. This study presents a regional-scale comparison of pollen and plant richness from northern Europe and evaluates the importance of environmental variables on pollen and plant richness. We use a pollen dataset of 511 lake-surface pollen samples ranging through temperate, boreal and tundra biomes. To characterise plant diversity, we use a dataset formulated from the two largest plant atlases available in Europe. We compare pollen and plant richness estimates in different groups of taxa (wind-pollinated vs. non-wind-pollinated, trees and shrubs vs. herbs and grasses) and test their relationships with climate and landscape variables. Pollen richness is significantly positively correlated with plant richness (r = 0.53). The pollen plant richness correlation improves (r = 0.63) when high pollen producers are downweighted prior to estimating richness minimising the influence of pollen production on the pollen richness estimate. This suggests that methods accommodating pollen-production differences in richness estimates deserve further attention and should become more widely used in Quaternary pollen diversity studies. The highest correlations are found between pollen and plant richness of trees and shrubs (r = 0.83) and of wind-pollinated taxa (r = 0.75) suggesting that these are the best measures of broad-scale plant richness over several thousands of square kilometres. Mean annual temperature is the strongest predictor of both pollen and plant richness. Landscape openness is positively associated with pollen richness but not with plant richness. Pollen richness values from extremely open and/or cold areas where pollen production is low should be interpreted with caution because low local pollen production increases the proportion of extra-regional pollen. Synthesis. Our results confirm that pollen data can provide insights into past plant richness changes in northern Europe, and with careful consideration of pollen-production differences and spatial scale represented, pollen data make it possible to investigate vegetation diversity trends over long time-scales and under changing climatic and habitat conditions.Peer reviewe

Plant Responses to Extreme Climatic Events: A Field Test of Resilience Capacity at the Southern Range Edge

The expected and already observed increment in frequency of extreme climatic events may result in severe vegetation shifts. However, stabilizing mechanisms promoting community resilience can buffer the lasting impact of extreme events. The present work analyzes the resilience of a Mediterranean mountain ecosystem after an extreme drought in 2005, examining shoot-growth and needle-length resistance and resilience of dominant tree and shrub species (Pinus sylvestris vs Juniperus communis, and P. nigra vs J. oxycedrus) in two contrasting altitudinal ranges. Recorded high vegetative-resilience values indicate great tolerance to extreme droughts for the dominant species of pine-juniper woodlands. Observed tolerance could act as a stabilizing mechanism in rear range edges, such as the Mediterranean basin, where extreme events are predicted to be more detrimental and recurrent. However, resistance and resilience components vary across species, sites, and ontogenetic states: adult Pinus showed higher growth resistance than did adult Juniperus; saplings displayed higher recovery rates than did conspecific adults; and P. nigra saplings displayed higher resilience than did P. sylvestris saplings where the two species coexist. P. nigra and J. oxycedrus saplings at high and low elevations, respectively, were the most resilient at all the locations studied. Under recurrent extreme droughts, these species-specific differences in resistance and resilience could promote changes in vegetation structure and composition, even in areas with high tolerance to dry conditions.This study was supported by Ministerio de Ciencia e Innovación (Spanish Government) Projects CGL2008-04794 and CGL2011-29910 to R.Z., and by grant FPU-MEC (AP2005-1561) to A. H