A New Low-Cost Microfluidics Device & Microscope

https://openworks.mdanderson.org/sumexp21/1191/thumbnail.jp

Latent states extraction through Kalman Filter for the prediction of heart failure decompensation events

[EN] Cardiac function deterioration of heart failure patients is frequently manifested by the occurrence of decompensation events. One relevant step to adequately prevent cardiovascular status degradation is to predict decompensation episodes in order to allow preventive medical interventions.In this paper we introduce a methodology with the goal of finding onsets of worsening progressions from multiple physiological parameters which may have predictive value in decompensation events. The best performance was obtained for the model composed by only two features using a telemonitoring dataset (myHeart) with 41 patients. Results were achieved by applying leave-one-subject-out validation and correspond to a geometric mean of 83.67%. The obtained performance suggests that the methodology has the potential to be used in decision support solutions and assist in the prevention of this public health burden.The authors acknowledge the financial support of the international project Link (H2020-692023).Nunes, D.; Rocha, T.; Traver Salcedo, V.; Teixeira, C.; Ruano, M.; Paredes, S.; Carvalho, P.... (2019). Latent states extraction through Kalman Filter for the prediction of heart failure decompensation events. IEEE. 3947-3950. https://doi.org/10.1109/EMBC.2019.8857591S3947395

Frequency response of IPMC actuators: physical characterization and identification for control

[Abstract] Ionic polymer metal composite (IPMC) actuators have promising applications in robotics and medicine in a not distance future, which will require a big knowledge in different fields, such as, manufacturing, material characterization and control theory. In this paper, frequency response of several IPMC actuators, cut from the same bulk IPMC sheet with a micro laser etching machine, is analyzed. Specifically, the objective is to 1) characterize each actuator, i.e., determine how cutting affects to the parameters of the physical model of this kind of actuators, and 2) identify a model for each actuator for control purposes. The frequency responses have been obtained experimentally in LabVIEW by attaching a couple of gold electrodes to each IPMC unit and measuring the tip deection by means of a laser distance meter.[Resumen] Los actuadores de compuesto de polímero metálico iónico (IPMC) tienen aplicaciones prometedoras en robótica y medicina en un futuro no lejano, lo que requerirá un gran conocimiento en diferentes campos, como la fabricación, la caracterización de materiales y la teoría de control. En este documento, se analiza la respuesta de frecuencia de varios actuadores IPMC, cortados de la misma hoja de IPMC a granel con una máquina de grabado por láser micro. Específicamente, el objetivo es 1) caracterizar cada actuador, es decir, determinar cómo afecta el corte a los parámetros del modelo físico de este tipo de actuadores, y 2) identificar un modelo para cada actuador con fines de control. Las respuestas de frecuencia se han obtenido experimentalmente en LabVIEW al conectar un par de electrodos de oro a cada unidad IPMC y medir la división de la punta por medio de un medidor de distancia láser.Ministerio de Economía y Competitividad; DPI2016-80547-

HoughNet: Integrating Near and Long-Range Evidence for Bottom-Up Object Detection

© 2020, Springer Nature Switzerland AG.This paper presents HoughNet, a one-stage, anchor-free, voting-based, bottom-up object detection method. Inspired by the Generalized Hough Transform, HoughNet determines the presence of an object at a certain location by the sum of the votes cast on that location. Votes are collected from both near and long-distance locations based on a log-polar vote field. Thanks to this voting mechanism, HoughNet is able to integrate both near and long-range, class-conditional evidence for visual recognition, thereby generalizing and enhancing current object detection methodology, which typically relies on only local evidence. On the COCO dataset, HoughNet’s best model achieves 46.4 AP (and 65.1 AP50), performing on par with the state-of-the-art in bottom-up object detection and outperforming most major one-stage and two-stage methods. We further validate the effectiveness of our proposal in another task, namely, “labels to photo” image generation by integrating the voting module of HoughNet to two different GAN models and showing that the accuracy is significantly improved in both cases. Code is available at https://github.com/nerminsamet/houghnet

The cost of promiscuity: sexual transmission of Nosema microsporidian parasites in polyandrous honey bees

Multiple mating (and insemination) by females with different males, polyandry, is widespread across animals, due to material and/or genetic benefits for females. It reaches particularly high levels in some social insects, in which queens can produce significantly fitter colonies by being polyandrous. It is therefore a paradox that two thirds of eusocial hymenopteran insects appear to be exclusively monandrous, in spite of the fitness benefits that polyandry could provide. One possible cost of polyandry could be sexually transmitted parasites, but evidence for these in social insects is extremely limited. Here we show that two different species of Nosema microsporidian parasites can transmit sexually in the honey bee Apis mellifera. Honey bee males that are infected by the parasite have Nosema spores in their semen, and queens artificially inseminated with either Nosema spores or the semen of Nosema-infected males became infected by the parasite. The emergent and more virulent N. ceranae achieved much higher rates of infection following insemination than did N. apis. The results provide the first quantitative evidence of a sexually transmitted disease (STD) in social insects, indicating that STDs may represent a potential cost of polyandry in social insects

CREB is a critical regulator of normal hematopoiesis and leukemogenesis

The cAMP-responsive element binding protein (CREB) is a 43-kDa nuclear transcription factor that regulates cell growth, memory, and glucose homeostasis. We showed previously that CREB is amplified in myeloid leukemia blasts and expressed at higher levels in leukemia stem cells from patients with myeloid leukemia. CREB transgenic mice develop myeloproliferative disease after 1 year, but not leukemia, suggesting that CREB contributes to but is not sufficient for leukemogenesis. Here, we show that CREB is most highly expressed in lineage negative hematopoietic stem cells (HSCs). To understand the role of CREB in hematopoietic progenitors and leukemia cells, we examined the effects of RNA interference (RNAi) to knock down CREB expression in vitro and in vivo. Transduction of primary HSCs or myeloid leukemia cells with lentiviral CREB shRNAs resulted in decreased proliferation of stem cells, cell- cycle abnormalities, and inhibition of CREB transcription. Mice that received transplants of bone marrow transduced with CREB shRNA had decreased committed progenitors compared with control mice. Mice injected with Ba/F3 cells expressing either Bcr-Abl wild-type or T315I mutation with CREB shRNA had delayed leukemic infiltration by bioluminescence imaging and prolonged median survival. Our results suggest that CREB is critical for normal myelopoiesis and leukemia cell proliferation

Integrated seismic ambient noise, magnetotellurics and gravity data for the 2D interpretation of the Vallès basin structure in the geothermal system of La Garriga-Samalús (NE Spain)

The integration of geophysical methods, together with the previous information of the Vallès basin area, has resulted in the creation of a new conceptual model that explains La Garriga-Samalús geothermal system. The integration of complementary geophysical methods seems to be a good option for the preliminary stages of a geothermal system exploration, especially in urban areas. An integrated seismic ambient noise, magnetotellurics, and gravity methods were used to determine the geological units and structures which control the La Garriga-Samalús geothermal system. The 2D resistivity and density models have allowed the identification of the four main units which regulate the geothermal system: the Miocene basin, the Prelitoral Range unit, the Vallès Faut Zone, and the Paleozoic basement. The interpretation of our models set the Vallès Fault Zone, which is characterized by an anomalous low resistivity and low density, as the main path for the hot fluids. Moreover, the geophysical characterization established a new geometry for the Miocene basin. The Miocene basin presents a stepwise morphology, with the minor thickness towards the fault and an increasing thickness towards the center of the basin. This geometry seems to be related to synthetic normal faults. These results have evidenced that, although, in some geothermal systems, the warm water may create an insufficient physical contrast; the appropriate use of some techniques can still be useful for the exploration of medium and low-temperature geothermal systems