Application of frozen thin sectioning immunogold staining to the study of the developing neuroepithelial basal lamina

In order to examine the deposition of basal lamina components in the developing neurocpithelium, a technique for frozen thin sectioning and immunogold staining of early embryonic tissue was developed. Different fixatives and buffer systems were evaluated to determine which best retained immunoreactivity and satisfactory ultrastructure of day 9 and 10 mouse embryos. Fixation in sodium phosphate and sodium bicarbonate buffers did not retain antigenicity, and incubations in TBS ( Tris hydroxymethyl-aminomethane buffered saline) in an effort to ‘restore’ immunoreactivity were similarly unsuccessful. Fixation in sodium cacodylate buffer, however, did retain the antigenicity of basal lamina components; the pattern of type IV collagen and laminin distribution was clearly determined. These results represent the first report of on-grid immunocytochemistry of carly embryonic material.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47407/1/418_2004_Article_BF00518729.pd

Ökologische Untersuchungen zur Nitrifikation in Nord-und Ostsee

Ammonia, nitrite and nitrate were regularly estimated at several stations in the Kieler Bucht (western Baltic Sea) since November 1964. There are considerable seasonal changes in the contents of these 3 nitrogen compounds with impressive maxima of nitrite and nitrate in February or at the beginning of March. The great increase of nitrite and nitrate during the winter and also a smaller increase in summer are mainly caused by oxidation of ammonia, first to nitrite and then to nitrate, by nitrifying bacteria. In consequence chemoautotrophic nitrite- and nitratebacteria could be found in the water as well as in sediments all over the Kieler Bucht and also in the North Sea around the isle of Helgoland. These nitrifying bacteria are able to oxidize ammonia or nitrite in salinity conditions typical for the western Baltic Sea and the North Sea

Soil physical properties affected by soil management and crop rotation in a long term experiment in Southern Brazil

The main objective of this work is to evaluate soil physical properties affected by cover crop rotations and soil management after 19 years of applying NoTill and Conventional Tillage systems with different winter species on a clayey Oxisol in South Brazil

Adubação nitrogenada em videiras jovens e em fase produtiva: recuperação e distribuição na planta do nitrogênio adicionado no solo.

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Flutuação populacional da Mosca-Branca (Bemisia tuberculata), Percevejo-de-Renda (Vatiga manihotae) e Tripes (Frankliniella sp.) na mandioca no Paraná e Mato Grosso do Sul.

A mandioca (Manihot esculenta) representa importante fonte de carboidratos compondo a base energética da alimentação de comunidades, sobretudo nas regiões tropicais (TAKAHASHI e GONÇALO, 2005). Além disso, no Brasil, constitui importante fonte de matéria-prima para produtos agroindustrializados, notadamente nos estados do Sul e Sudeste (TAKAHASHI, 2002). A produção agrícola industrial do país tem se intensificado na região Centro-Sul do país, principalmente no Paraná e Mato Grosso do Sul, onde se concentra o plantio da cultura em novas áreas (GROXKO, 2010).Fitosanidade. Resumo n. 166

Cobre em solos cultivados com a videira na Serra Gaúcha do Rio Grande do Sul.

O presente trabalho objetivou determinar as formas de cobre e a cinética de dessorção em solos cultivados com a videira e submetidos a sucessivas aplicações de fungicidas cúpricos.bitstream/CNPUV/10103/1/cot086.pd

Investigation of the occurrence of pesticide residues in rural wells and surface water following application to tobacco

In this work the exposure of wells and surface water to pesticides, commonly used for tobacco cropping, was assessed. Water consumption wells and surface water flows were sampled at different times. After a preconcentration step with solid phase extraction (SPE), the selected pesticides were determined by gas chromatography with electron capture detection (GC-ECD) or high performance liquid chromatography with diode array detection (HPLC-DAD). No pesticides were detected in the well water samples and surface water flow in the winter season. However, in the spring and summer higher concentrations of chlorpyrifos and imidacloprid were found in the water source samples. Atrazine, simazine and clomazone were also found. The occurrence of pesticides in collected water samples was related with the application to tobacco

Mineralogical changes caused by grape production in a regosol from subtropical Brazilian climate.

Purpose Inadequate soil use and management practices promote commonly negative impacts on the soil constituents and their properties, with consequences to ecosystems. As the soil mineralogy can be permanently altered due to soil use, this approach can be used as a tool to monitor the anthropogenic pressure. The objective of the present study was to assess the mineralogical alterations of a Brazilian regosol used for grape production for 40 years in comparison with a soil under natural vegetation (forest), aiming to discuss anthropogenic pressure on soils. Material and methods Soil samples were collected at depths of 0?0.20 and 0.20?0.40 m from vineyard production and natural vegetation sites. Physical and chemical parameters were analysed by classic approaches. Mineralogical analyses were carried out on <2 mm, silt and clay fractions. Clay minerals were estimated by the relative percentage of peak surface area of the X-ray patterns. Results and discussion Grape production reduced the organic matter content by 28% and the clay content by 23% resulting in a decreasing cation exchange capacity. A similar clay fraction was observed in both soils, containing kaolinite, illite/mica and vermiculite with hydroxy-Al polymers interlayered. Neither gibbsite nor chlorite was found. However, in the soil under native vegetation, the proportion of illite (79 %) was higher than vermiculite (21 %). Whereas, in the soil used for grape production during 40 years, the formation of vermiculite was promoted. Conclusions Grape production alters the proportions of soil constituents of the regosol, reducing clay fraction and organic matter contents, as well as promoting changes in the soil clay minerals with the formation of vermiculite to the detriment of illite, which suggests weathering acceleration and susceptibility to anthropogenic pressure. Recommendations and perspectives Ecosystems in tropical and subtropical climates can be more easily and permanently altered due to anthropogenic pressure, mainly as a consequence of a great magnitude of phenomena such as temperature amplitude and rainfall that occurs in these regions. This is more worrying when soils are located on steep grades with a high anthropogenic pressure, like regosols in Southern Brazil. Thus, this study suggests that changes in soil mineralogy can be used as an important tool to assess anthropogenic pressure in ecosystems and that soil quality maintenance should be a priority in sensible landscapes to maintain the ecosystem quality

miR-34a: a new player in the regulation of T cell function by modulation of NF-κB signaling

NF-κB functions as modulator of T cell receptor-mediated signaling and transcriptional regulator of miR-34a. Our in silico analysis revealed that miR-34a impacts the NF-κB signalosome with miR-34a binding sites in 14 key members of the NF-κB signaling pathway. Functional analysis identified five target genes of miR-34a including PLCG1, CD3E, PIK3CB, TAB2, and NFΚBIA. Overexpression of miR-34a in CD4+ and CD8+ T cells led to a significant decrease of NFΚBIA as the most downstream cytoplasmic NF-κB member, a reduced cell surface abundance of TCRA and CD3E, and to a reduction of T cell killing capacity. Inhibition of miR-34a caused an increase of NFΚBIA, TCRA, and CD3E. Notably, activation of CD4+ and CD8+ T cells entrails a gradual increase of miR-34a. Our results lend further support to a model with miR-34a as a central NF-κB regulator in T cells