Reactive oxygen species-driven transcription in Arabidopsis under oxygen deprivation

Reactive oxygen species (ROS) play an important role as triggers of gene expression during biotic and abiotic stresses, among which is low oxygen (O2). Previous studies have shown that ROS regulation under low O2 is driven by a RHO-like GTPase that allows tight control of hydrogen peroxide (H2O2) production. H2O2 is thought to regulate the expression of heat shock proteins, in a mechanism that is common to both O2 deprivation and to heat stress. In this work, we used publicly available Arabidopsis (Arabidopsis thaliana) microarray datasets related to ROS and O2 deprivation to define transcriptome convergence pattern. Our results show that although Arabidopsis response to anoxic and hypoxic treatments share a common core of genes related to the anaerobic metabolism, they differ in terms of ROS-related gene response. We propose that H2O2 production under O2 deprivation is a trait present in a very early phase of anoxia, and that ROS are needed for the regulation of a set of genes belonging to the heat shock protein and ROS-mediated groups. This mechanism, likely not regulated via the N-end rule pathway for O2 sensing, is probably mediated by a NADPH oxidase and it is involved in plant tolerance to the stress

Dust attenuation law in JWST galaxies at z = 7-8

Attenuation curves in galaxies depend on dust chemical composition, content, and grain size distribution. Such parameters are related to intrinsic galaxy properties such as metallicity, star formation rate, and stellar age. Due to the lack of observational constraints at high redshift, dust empirical curves measured in the local Universe (e.g. Calzetti and SMC curves) have been employed to describe the dust attenuation at early epochs. We exploit the high sensitivity and spectral resolution of the JWST to constrain the dust attenuation curves in high-z galaxies. Our goals are to check whether dust attenuation curves evolve with redshift and quantify the dependence of the inferred galaxy properties on the assumed dust attenuation law. We develop a modified version of the SED fitting code BAGPIPES by including a detailed dust attenuation curve parametrization. Dust parameters are derived, along with galaxy properties, from the fit to the data from FUV to mm bands. Once applied to three star-forming galaxies at z = 7-8, we find that their attenuation curves differ from local templates. One out of three galaxies shows a characteristic MW bump, typically associated to the presence of small carbonaceous dust grains such as PAHs. This is one of the first evidences suggesting the presence of PAHs in early galaxies. Galaxy properties such as stellar mass and SFR inferred from SED fitting are strongly affected by the assumed attenuation curve, though the adopted star formation history also plays a major role. Our results highlight the importance of accounting for the potential diversity of dust attenuation laws when analyzing the properties of galaxies at the EoR, whose dust properties are still poorly understood. The application of our method to a larger sample of galaxies observed with JWST can provide us important insights into the properties of dust and galaxies in the early universe.Comment: 19 pages, 10 figure

Ultrastructural Characterization of the Lower Motor System in a Mouse Model of Krabbe Disease

Krabbe disease (KD) is a neurodegenerative disorder caused by the lack of β- galactosylceramidase enzymatic activity and by widespread accumulation of the cytotoxic galactosyl-sphingosine in neuronal, myelinating and endothelial cells. Despite the wide use of Twitcher mice as experimental model for KD, the ultrastructure of this model is partial and mainly addressing peripheral nerves. More details are requested to elucidate the basis of the motor defects, which are the first to appear during KD onset. Here we use transmission electron microscopy (TEM) to focus on the alterations produced by KD in the lower motor system at postnatal day 15 (P15), a nearly asymptomatic stage, and in the juvenile P30 mouse. We find mild effects on motorneuron soma, severe ones on sciatic nerves and very severe effects on nerve terminals and neuromuscular junctions at P30, with peripheral damage being already detectable at P15. Finally, we find that the gastrocnemius muscle undergoes atrophy and structural changes that are independent of denervation at P15. Our data further characterize the ultrastructural analysis of the KD mouse model, and support recent theories of a dying-back mechanism for neuronal degeneration, which is independent of demyelination

ALMA hints at the presence of turbulent disk galaxies at z > 5

High-redshift galaxies are expected to be more turbulent than local galaxies because of their smaller size and higher star formation and thus stronger feedback from star formation, frequent mergers events, and gravitational instabilities. However, this scenario has recently been questioned by the observational evidence of a few galaxies at z~4-5 with a gas velocity dispersion similar to what is observed in the local population. Our goal is to determine whether galaxies in the first Gyrs of the Universe have already formed a dynamically cold rotating disk similar to the local counterparts. We studied the gas kinematic of 22 main-sequence star-forming galaxies at z > 5 and determined their dynamical state by estimating the ratio of the rotational velocity and of the gas velocity dispersion. We mined the ALMA archive and exploited the [CII] and [OIII] observations to perform a kinematic analysis of the cold and warm gas of z>5 main-sequence galaxies. The gas kinematics of the high-z galaxies is consistent within the errors with rotating but turbulent disks. We infer a velocity dispersion that is systematically higher by 4 times than the local galaxy population and the z~5 dust-obscured galaxies reported in the literature. The difference between our results and those reported at similar redshift can be ascribed to the systematic difference in the galaxy properties in the two samples: the disks of massive dusty galaxies are dynamically colder than the disks of dust-poor galaxies. The comparison with the theoretical predictions suggests that the main driver of the velocity dispersion in high-z galaxies is the gravitational energy that is released by the transport of mass within the disk. Finally, we stress that future deeper ALMA high-angular resolution observations are crucial to constrain the kinematic properties of high-z galaxies and to distinguish rotating disks from kpc-scale mergers.Comment: 14 pages, 11 figures, 1 tables, accepted for publication in A&

Teoría de Grafos para la Identificación de Nodos Maliciosos en la Red

Se explora el reconocimiento de las botnets en una red a partir de su representación como grafo, extrayendo características a sus nodos y poniendo a prueba algoritmos de agrupamiento. Se logra la separación del 88% de las botnets junto al -0,14% de los nodos benignos.XI Workshop Seguridad Informática (WSI)Red de Universidades con Carreras en Informátic

Aquatic Organic Matter in the Seine Basin: Sources, Spatio-Temporal Variability, Impact of Urban Discharges and Influence on Micro-pollutant Speciation

International audienc

GA-NIFS: co-evolution within a highly star-forming galaxy group at z=3.7 witnessed by JWST/NIRSpec IFS

We present NIRSpec IFS observations of a galaxy group around the massive GS_4891 galaxy at z=3.7 in GOODS-South that includes two other two systems, GS_4891_n to the north and GS_28356 to the east. These observations, obtained as part of the GTO GA-NIFS program, allow for the first time to study the spatially resolved properties of the interstellar medium (ISM) and ionized gas kinematics of a galaxy at this redshift. Leveraging the wide wavelength range spanned with the high-dispersion grating (with resolving power R=2700) observations, covering from [OII]$\lambda$$\lambda$3726,29 to [SII]$\lambda$$\lambda$6716,31, we explore the spatial distribution of star-formation rate, nebular attenuation and gas metallicity, together with the mechanisms responsible for the excitation of the ionized gas. GS_4891 presents a clear gradient of gas metallicity (as traced by 12 + log(O/H)) by more than 0.2dex from the south-east (where a star-forming clump is identified) to the north-west. The gas metallicity in the less-massive northern system, GS_4891_n, is also higher by 0.2 dex than at the center of GS_4891, suggesting that inflows of lower-metallicity gas might be favoured in higher-mass systems. The kinematic analysis shows that GS_4891 presents velocity gradients in the ionized gas consistent with rotation. The region between GS_4891 and GS_4891_n does not present high gas turbulence which, together with the difference in gas metallicities, suggests that these two systems might be in a pre-merger stage. Finally, GS_4891 hosts an ionized outflow that extends out to r_out=1.2 kpc from the nucleus and reaches maximum velocities v_out of approximately 400 km/s. Despite entraining an outflowing mass rate of M_out$\sim$2Msun/yr, the low associated mass-loading factor, $\eta$=0.05, implies that the outflow does not have a significant impact on the star-formation activity of the galaxy.Comment: Submitted to Astronomy & Astrophysics on September 25th, 202

Removal of Uracil by Uracil DNA Glycosylase Limits Pemetrexed Cytotoxicity: Overriding the Limit with Methoxyamine to Inhibit Base Excision Repair

Uracil DNA glycosylase (UDG) specifically removes uracil bases from DNA, and its repair activity determines the sensitivity of the cell to anticancer agents that are capable of introducing uracil into DNA. In the present study, the participation of UDG in the response to pemetrexed-induced incorporation of uracil into DNA was studied using isogenic human tumor cell lines with or without UDG (UDG+/+/UDG−/−). UDG−/− cells were very sensitive to pemetrexed. Cell killing by pemetrexed was associated with genomic uracil accumulation, stalled DNA replication, and catastrophic DNA strand breaks. By contrast, UDG+/+ cells were \u3e10 times more resistant to pemetrexed due to the rapid removal of uracil from DNA by UDG and subsequent repair of the resultant AP sites (abasic sites) via the base excision repair (BER). The resistance to pemetrexed in UDG+/+ cells could be reversed by the addition of methoxyamine (MX), which binds to AP sites and interrupts BER pathway. Furthermore, MX-bound AP sites induced cell death was related to their cytotoxic effect of dual inactivation of UDG and topoisomerase IIα, two genes that are highly expressed in lung cancer cells in comparison with normal cells. Thus, targeting BER-based therapy exhibits more selective cytotoxicity on cancer cells through a synthetic lethal mechanism

Distinct mechanisms for aerenchyma formation in leaf sheaths of rice genotypes displaying a quiescence or escape strategy for flooding tolerance

Background and Aims Rice is one of the few crops able to withstand periods of partial or even complete submergence. One of the adaptive traits of rice is the constitutive presence and further development of aerenchyma which enables oxygen to be transported to submerged organs. The development of lysigenous aerenchyma is promoted by ethylene accumulating within the submerged plant tissues, although other signalling mechanisms may also co-exist. In this study, aerenchyma development was analysed in two rice (Oryza sativa) varieties, ‘FR13A’ and ‘Arborio Precoce’, which show opposite traits in flooding response in terms of internode elongation and survival. Methods The growth and survival of rice varieties under submergence was investigated in the leaf sheath of ‘FR13A’ and ‘Arborio Precoce’. The possible involvement of ethylene and reactive oxygen species (ROS) was evaluated in relation to aerenchyma formation. Cell viability and DNA fragmentation were determined by FDA/FM4-64 staining and TUNEL assay, respectively. Ethylene production was monitored by gas chromatography and by analysing ACO gene expression. ROS production was measured by using Amplex Red assay kit and the fluorescent dye DCFH2-DA. The expression of APX1 was also evaluated. AVG and DPI solutions were used to test the effect of inhibiting ethylene biosynthesis and ROS production, respectively. Key Results Both the varieties displayed constitutive lysigenous aerenchyma formation, which was further enhanced when submerged. ‘Arborio Precoce’, which is characterized by fast elongation when submerged, showed active ethylene biosynthetic machinery associated with increased aerenchymatous areas. ‘FR13A’, which harbours the Sub1A gene that limits growth during oxygen deprivation, did not show any increase in ethylene production after submersion but still displayed increased aerenchyma. Hydrogen peroxide levels increased in ‘FR13A’ but not in ‘Arborio Precoce’. Conclusions While ethylene controls aerenchyma formation in the fast-elongating ‘Arborio Precoce’ variety, in ‘FR13A’ ROS accumulation plays an important role